• Journal of the Computational Structural Engineering Institute of Korea
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• v.21 no.3
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• pp.287-294
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• 2008

This study proposes a novel method for in service evaluation of tension force of a prestressed 7-wire strand which is frequently employed for retrofitting bridge superstructure. The smart strand is made by replacing the straight king wire of the strand with an instrumented steel tube in which the FBG sensor is embedded. Since the strain of the smart strand can easily be measured using the sensor, it is possible to monitor tension force of the strand during the service. For the sake of demonstrating effectiveness of the proposed strand, we came up with a 7.0m long prototype with 2 FBG sensors, and it is applied as an external tendon to a 6.4m long and 0.6 high RC T-shaped beam. A loading-unloading test has been carried out, and estimated tension forces using the smart strand are compared with measured forces by load cell. The comparison showed that the proposed smart tendon is useful and accurate for monitering tension force of the prestressed tendon.

• Elastomers and Composites
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• v.42 no.3
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• pp.168-176
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• 2007

When the rubber vulcanizates reinforced with carbon black or silica are subjected to cyclic loading from its virgin state, the stress required on reloading is less than that on the initial loading. This stress softening phenomenon is referred to as the Mullins effect. The strain energy function of rubber vulcanizates was investigated using theory of pseudo-elasticity incorporated damage parameter that Ogden and Roxburgh have proposed to describe the damage-induced stress softening effect in rubber-like solids. The quasi-static cyclic loading test was performed using the NR-SBR vulcanizates reinforced with carbon black, and then the effect of a damage parameter to stress-strain curve in reloading and subsequent reloading paths was studied. The strain energy function of the rubber vulcanizates with a different filler content was also evaluated.

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.9
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• pp.926-934
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• 2006

This paper proposes intelligent control of a virtual walking machine that can generate infinite floor for various surfaces and can provide proprioceptive feedback of walking to a user. This machine allows users to participate in a life-like walking experience in virtual environments with various terrains. The controller of the machine is implemented hierarchically, at low-level for robust actuator control, at mid-level fur platform control to compensate the external forces by foot contact, and at high-level control for generating walking trajectory. The high level controller is suggested to generate continuous walking on an infinite floor for various terrains. For the high level control, each independent platform follows a man foot during the swing phase, while the other platform moves back during single stance phase. During double limb support, two platforms manipulate neutral positions to compensate the offset errors generated by velocity changes. This control can, therefore, satisfy natural walking conditions in any direction. Transition phase between the swing and the stance phases is detected by using simple switch sensor system, while human foot motions are sensed by careful calibration with a magnetic motion tracker attached to the shoe. Experimental results of walking simulations at level ground, slope, and stairs, show that with the proposed machine, a general person can walk naturally on various terrains with safety and without any considerable disturbances. This interface can be applied to various areas such as VR navigations, rehabilitation, and gait analysis.

• Journal of the Korean Chemical Society
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• v.40 no.9
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• pp.623-629
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• 1996

In order to identify antibiotic substances in the extract of a lichen, Umbilicaria vellea, the extract was chromatographed and two compounds were isolated. Compound I which showed antifungal and antibacterial activities, melted around 129∼132$^{\circ}C$ and showed UV absorption at 217, 265 and 300 nm. It showed a molecular ion at m/z 196. Its molecular formular was confirmed to be $C_{10}H_{12}O_4$ from elemental analysis. From its IR and NMR data it was identified to be ethyl 2,4-dihydroxy-6-methyl benzoate. Compound II melted around 58∼59$^{\circ}C$ and showed UV absorption at 212, 276 and 282 nm. It showed a molecular ion at m/z 124 and molecular formular was confirmed to be $C_7H_8O_2.$ From the analysis of its IR and NMR spectra it was identified to be 5-methyl-1,3-benzenediol.

• Journal of Life Science
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• v.13 no.3
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• pp.308-313
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• 2003

The collagenase gene from Vibrio parahaemolyticus 04 was subcloned into an expression vector pET-29b. The recombinant collagenase was expressed in Escherichia coli BL2l(DE3) and partially purified by Hi-Trap affinity and Sephacryl S-100 size exclusion chromatographies. The recombinant enzyme was purified by 43.7-fold and the yield was 73%. SDS-PAGE revealed that the molecular weight of the enzyme was approximately 35 kDa. Substrate specificity study of the enzyme displayed that the enzyme showed the highest activity with the type I collagen and the synthetic peptide, Z-GPGGPA, indicating that the enzyme was indeed a collagenase. The enzyme showed broad pH optimum around pH 6-12 and was stable between pH 5.5 and 11.5. The optimum temperature for the type I collagen degradation was $35^{\circ}C$. The thermostability measurement of the enzyme indicated that the enzyme was stable up to $55^{\circ}C$, but the activity was diminished quickly above $60^{\circ}C.$

• Journal of the Korean Geotechnical Society
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• v.19 no.3
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• pp.93-104
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• 2003

The Disturbed State Concept (DSC) model, with simplified unloading/reloading formulation, is implemented in a nonlinear dynamic finite element program fur porous media named DSC_DYN2D. In this research, the DSC constitutive model is utilized using the HiSS model for relative intact (RI) part and the critical state model for the fully adjusted (FA) part in the material. The general formulation for implementation is developed. The cyclic loading tests from the field load test data on a pile segment were numerically simulated using the finite element program DSC_DYN2D and compared with field measurements and those from the previous analysis with the HiSS model. The DSC predictions show improved agreement with the field behavior of the pile compared to those from the HiSS model. Overall, the computer procedure with the DSC model allows improved and realistic simulation of the complex dynamic soil-structure interaction problems.

• KSBB Journal
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• v.15 no.5
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• pp.537-540
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• 2000

In developing a HPLC purification process, it was hoped that a single chromatographic system would be sufficient to abtain pure paclitaxel in high yield. However, no such system was found, due in part to the complex taxoid profile of crude paclitaxel and to the rigorous nature of the product specification. A two step HPLC purification was adopted using reverse-phase separation on C(sub)18 as a first step, and normal-phase separation on silica as the final polishing step. Impurity profiles were established and maintained for paclitaxel, which identified and quantified each impurity observed in purified paclitaxel from these two steps, all impurities at or above 0.1% were identified. Results provide information for improving the quality control of paclitaxel production.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.275-281
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• 1982

One strain of Streptomyces sp. (AS-707) isolated from soil was found to produce a biologically active substance that showed a strong inhibitory activity against proteolytic enzymes viz. trypsin, papain, $\alpha$-chymotrypsin, Azotobacter protease, and Bacillus pretense. The substance was separated from culture filtrate by ion exchange column chromatography using Amberlite IRC-50 and CM-cellulose column chromatography. It was found that the recovery yield was 26% as activity basis. The substance was stable in wide pH range from 2.0 to 12.0 at 37$^{\circ}C$, but it was unstable in alkaline pH values at 6$0^{\circ}C$. The activity was thermostable to give 90% activity compared to the intact sample when it was treated at pH5.6 at 10$0^{\circ}C$ for 2 hours.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.41 no.2
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• pp.229-237
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• 2016

The purpose of this paper is to empirically identify whether unit bias exists or not under the flat rate pricing in the Korean mobile telecommunication market and to give the desirable form of pricing plans for minimizing this irrational behaviors. Our results show that with the flat rate pricing consumers tends to make more voice or data traffic over their optimal consumption level, meaning the existence of unit bias in the Korean mobile market. These results imply that under the current pricing plans subscribers may pay higher monthly fee than their optimal cost which maximizes their utility, for using the telecommunications service. Thus, policy makers need to consider adopting mobile operators' segmentation of the flat rate pricing plans for the reduction of subscribers' telecommunications costs and the improvement of consumer welfare.

• KSBB Journal
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• v.10 no.3
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• pp.343-348
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• 1995

Human lipocorin-I(LCI) is a calcium ion-dependent and phospholipid-binding protein which exhibits an anti-inflammatory activity by inhibiting phospholipase A2 activity. In this study, the LCI gene containing its own terminator region was joined to GAL10 promoter-ppL (prepro-leader sequence of mating factor a). An ATG start codon of LCI gene was placed at downstream with KR endoprotease recognition site(Lys-Arg) of ppL. Recombinant S. cerevisiae harboring the LCI expression/secretion vector, pYGLPT5, was aerobicall grown on a liquid YPDG medium al $30^{\circ}C$ for 72hys. The whole cell and culture supernatant were separated after centrifugation, and the expressed LCI was analyzed by SDS-PAGE and western blotting methods. A majority fraction of the expressed LCI was found to be accumulated in the intracellular fraction, resulting in very low secretion efficiency of about 7.4%. About $500mg/\ell$ of LCI was extracellularly produced by the fed-batch culture employing the controlledfeeding of glucose and galactose. The secreted LCI was purified by ultrafiltration and hydroxylapatite column chromatography, and a purity of more than 99% was obtained.