• Korean Journal of Animal Reproduction
• /
• v.11 no.1
• /
• pp.42-62
• /
• 1987

본 실험은 성숙가토의 정자와 정장의 항원성을 조사하여 면역적 불임원인 규명에 필요한 기초자료를 얻고자 시도하였다. 인공질로 채취한 정액을 원심분리하여 얻은 정자와 정장을 항원으로 사용하였으며 항원성의 측정방법은 크로마토그라프에 의한 단백질 분리, SDS-PAGE, HPLC, 한천확산법, 전기영동, 수동적혈구응집방법 및 부동화시험이었다. 얻어진 결과는 다음과 같다. 1. SDS-PAGE 전기영동시 정상가토 정장에서 약 23개의 단백질이 분리되었고, 그중 분자량이 약 20,000되는 단백질부분이 정관절제 수술한 정장에서 나타나지 않았다. 2. 정상가토 정장을 HPLC를 이용한 분석에서 3개의 peaks을 볼 수 있었고, 정관절제수술한 것에서는 peak 1에 해당하는 단백질이 소실되었다. 3. 한천확산시험에서 정상정장은 이종혈청과는 4개의 침강선을 나타냈고, 전기영동에서는 7개의 침강선을 나타냈다. 4. 이종면역에서 정자 및 정장은 항체가 상승이 용이하였지만, 동종면역시는 추가면역이 필요하였으며 개체간의 역가차이를 보였다. 정자면역한 자성가토에서는 수동적혈구응집반응을 나타냈지만, 한천확산 및 전기영동반응은 보이지 않았고, 같은 처치를 받은 웅성가토에서도 역시 같은 반응양상을 나타냈다. 5. 동종 및 이종항혈청을 이용한 교차전기영동방법으로 정장의 항원적 구성요소를 구별할 수 있었다. 사출된 정액에서 분리된 정장은 일부 항원을 포함하고 있었으며 이는 성숙한 정자에서 기인한 것으로 사료되었다.

• Journal of Veterinary Clinics
• /
• v.24 no.4
• /
• pp.486-492
• /
• 2007

Seminal plasma(SP) is usually removed from semen that is to be cryopreserved. However, some reports indicate that SP has beneficial effects on spermatozoa during chilling and freezing. The purpose of this study was to determine the effect of SP on sperm survival by adding SP to the extender before cooling and freezing canine spermatozoa. In replicate experiments, ejaculates obtained from four healthy dogs(1-4 years old) of various breeds were pooled, centrifuged at $300{\times}g$ for 10 min at $25^{\circ}C$, and the supernatant of seminal plasma was decanted. Spermatozoa were suspended in egg yolk-Tris(EYT) buffer. The study comprised two experiments: [Exp 1] Sperm were suspended in EYT extender containing either 0, 20, 40, 80 or 100% SP and were slowly cooled to $4^{\circ}C$ for 2h or held at $25^{\circ}C$ as controls. Sperm concentration was adjusted to $2{\times}10^8/ml$. [Exp II] Sperm samples, each of which contained $1{\times}10^8/ml$, were assigned to nine groups to be frozen. In the first four groups, sperm in EYT containing either 20, 40, 80 or 100% SP were cooled to $4^{\circ}C$, then diluted to contain final concentrations of EYT+0.6M glycerol and then were frozen. The final concentrations of SP were 10, 20, 40 or 50%. In the other four groups, sperm in EYT alone were first cooled slowly to $4^{\circ}C$, then diluted to contain final concentrations of EYT+0.6M glycerol plus 10, 20, 40 or 50% SP and then were frozen. Spermatozoa, which chilled in EYT alone and diluted to contain final concentrations of EYT+0.6M glycerol without seminal plasma, and then frozen, was regarded as control. Spermatozoa were frozen at $25^{\circ}C/min$ of cooling rate in plastic straws that were suspended above liquid nitrogen and thawed in water at $38^{\circ}C$ for 1 min. Sperm survival was assayed by determining progressive motility and integrity of plasma and acrosome membranes. Progressive motility was determined by microscopic examination at $200{\times}$ magnification. Membrane integrity was assessed by use of a double fluorescent dye, and acrosome integrity by staining sperm with Pisum sativum agglutinin. The results of the first experiment showed that adding SP did not improve motility of spermatozoa compared to those incubated without SP regardless of temperature. The results of the second experiment showed that spermatozoa suspended in EYT+0.6M glycerol containing SP exhibited the higher progressive motility before being frozen(P<0.05). However, frozen-thawed spermatozoa that had suspended in EYT+0.6M glycerol containing SP showed the similar or lower viability(P<0.05). In summary, although seminal plasma did not affect spermatozoa that were chilled in EYT without cryoprotectant(CPA), addition of seminal plasma to EYT containing CPA did significantly improved progressive motility of canine spermatozoa that were chilled.

• Fashion & Textile Research Journal
• /
• v.1 no.3
• /
• pp.264-274
• /
• 1999

The thermal resistance of 60 men's suits for summer and winter was measured to determine their thermal characteristics and physical properties, including air permeability, weight, and thickness of the jackets and trousers consisted of the ensembles were measured to predict the thermal resistance of garments and ensembles. In this study, general physical properties of the men' suit ensembles were determined. In general, thickness and weight of winter ensembles were greater than those of summer ensembles. A factor which could distinguish the difference between summer and winter ensembles was the air permeability. The air permeability of summer ensembles was 3~6 times greater than those of winter ensembles. For the thermal characteristics, the thermal resistance of winter ensembles were higher than those of summer ensembles. When the wind was involved, the thermal resistance of both ensembles decreased up to 30%. In addition, the equations were developed to predict the thermal resistance of the garments and ensembles when there was no air velocity and the thermal resistance of the ensembles with air velocity of 1.2 m/sec. Looking at the equations, thickness, weight, and size of the garments were the definite factors that affect the thermal resistance of the samples.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 1994.04a
• /
• pp.102-102
• /
• 1994

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1994.04a
• /
• pp.252-252
• /
• 1994

현재 시판되고 있는 정장용 생균 제제에 함유되어있는 정장균주의 하나인 Bifidohacterium bifidum은 항결핵제 중 rifampicin에 감수성으로 rifimpicin과 병용 투여시 본래의 정장 효과를 기대할 수 없다. 따라서, rifampicin에 내성인 돌연변이 균주를 얻기 위해 B. bifidum을 N-methyl-N'-nitro-N-nitroso- -guanidine(MNNG)로 처리하여 rifampicin에 내성인 30종의 균주를 선별 하였고, rifampicin에 대한 Minimal Inhibitory Concentration(MIC)를 측정해 본 결과 내성이 1,000배 이상 상승하였다. 또한 rifampicin에 내성인 균주 RFR61을 자연 돌연변이시켜 ofloxacin에도 내성인 돌연변이 균주 20종을 선별 하였고, MIC를 측정한 결과 내성이 4배 이상 증가하였다. 또, fructose-6-phosphate phosphoketolase test를 실시해 본 결과, 모두 Bifidobacterium임이 확인되었다. 유기산 생성량을 측정하여 모균주의 유기산 생성량과 가장 유사한 3균주, B. bifidum RFRll, RFR21, RFR61 그리고 OFR9을 선별하였다. 이 네 균주의 E. coli 생육 억제능을 측정한 결과 모두 모균주와 유사한 E. coli 생육 억제능을 가지고 있었다. Rifampicin 내성균주들에 대하여 내성 유지 시험을 한 결과 복귀 돌연변이에 의해 내성이 소실될 가능성은 없는 것으로 여겨진다. 마지막으로, 내성 균주에 의한 rifampicin 불활성화 여부를 알아 본 결과 rifampicin이 불활성 화되지 않음을 알 수 있었다. 이상의 결과를 통해 본 연구실에서 개발한 B. bifidum RFR11, RFR21 그리고 RFR61 균주들은 rifampicin에 내성이며, B.bifidum OFR9은 rifampicin과 ofloxacin에 이중 내성을 갖는 균주로서 모균주와 유사한 생화학적 특성을 갖는 우수한 정장 세균으로 여겨진다.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1993.04a
• /
• pp.143-143
• /
• 1993

현재 시판되고 있는 정장용 생균 제제에 함유되어있는 정장 균주의 하나인 Bifidobacterium bifidum은 항결핵제 중 rifampicin에 감수성으로 rifampicin과 병용 투여시 본래의 정장 효과를 기대할 수없다. 따라서, rifampicin에 내성인 돌연변이 균주를 얻기 위해 B. bifidum을 N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)로 처리하여 rifampicin에 내성인 30 종의 균주를 선별하였고, rifampicin에 대한 Minimal Inhibitory Concentration (MIC)를 측정해 본 결과 내성이 1,000 배 이상 상승하였다. 균주 동정을 위하여 fructose-6-phosphate phosphoketolase test를 실시해 본 결과 Bifidobacterium임이 확인되었다. 이들 내성 균주들의 유기산 생산량을 측정하여 그 생산량이 모균주와 가장 유사한 3 종의 균주를 선발하였다. 이들에 대하여 Escherichia coli 생육 억제능을 시험해 본 결과 E. coil 생육 억제능이 모균주와 유사하였다. 또, rifampicin을 함유한 배지에서 돌연변이 균주를 배양시킨 경우 rifampicin이 안정한 상태로 잔존한 것을 알 수 있었다. 이것으로 보아 돌연변이 균주들은 rifampicin을 분해 또는 변형시키는 효소를 생산하지 않는다고 볼 수 있다. 이상의 결과로 본 연구에서 개발한 돌연변이 균주들, 즉 B. bifidum RFRll, RFR21 그리고 RFR61은 rifampicin에 내성이면서 모균주와 동일한 생화학적 특성을 갖는 정장 균주로 여겨진다.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
• /
• v.18 no.2
• /
• pp.130-137
• /
• 1989

본 논문은 1. 초청정기술의 현황, 2. 초청정장치의 요소기술, 3. 초청정장치의 개요, 4. 국부청정실의 기본계획에 대해 기술하고 있다. 1. 초청정기술의 현황에서는, 본 연구수행의 방향설정을 위해 미국과 일본의 관련 학${\cdot}$협회의 동향 및 최근에 발표된 논문에 근거한 초청정기술의 연구경향과 과제 등을 파악 분석했다. 2. 초청정장치의 요소기술에서는, 초청정장치 개발에 필요한 미립자제거, 환경조건과 오염제어 기술을 확립하기 위해, Filter와 System Ceiling 방식, 온${\cdot}$습도, 진동, 정전기제어 기술에 관한 문제점과 대책에 대해 검토했다. 3. 초청정장치의 개요에서는, 초청정장치로써 대표적인 클린벤취의 관련규격, 설계요점 등을 검토하여 설계를 위한 기초자료로 삼았다. 4. 국부청정실의 기본계획에서는 상기의 요소기술과 기초자료에 근거하여 본 연구를 수행하기 위한 Line 형의 클린터널 유닛의 기본계획을 수립했다.

• Korean Journal of Animal Reproduction
• /
• v.11 no.1
• /
• pp.63-72
• /
• 1987

Effects of sperm and seminal plasma isoant ibodies upon the rate of superovulation and ferlil ization were slud ied in both normal and immunized rabbits. The results obstained were summarized as follows: 1. On examin.ltion of the superovulation in the immunized animals, the average number of ovulation points was 22.1 and 25.3 for spermtreated animals and for seminal plasma-treated animals, respec tively. As compared to the con¬trol group of 41.0 in number, the immunized showed statisfical significance in ovulation (P<0.05). 2. In ovary weight and follicle's size there were no significant differences among the three groups, whereas sperm-and seminal plasma¬treated groups had an average rate of fertiliza¬tion of 62.X% and 5X .0%, respectively, in re¬markable contrast to the control hTfOUP of 91.4'1'0 (P<0.05). 3. When the animals were inseminated with a mixture of semen plus sperm or seminal plasma antisera, a sharp reduction of fertilization was observed with 5.6% and 16.0% as compared to the control group (P<0.05). Consequently, immunization with either sperm or seminal plasma had a substantial effect on fertilization.

• The Research Journal of the Costume Culture
• /
• v.11 no.1
• /
• pp.1-10
• /
• 2003

The purpose of this study is to identify differences that married exist between married women´s buying motivations for imported and domestic formal two piece suits and to test the role of socioeconomic variables on those differences. Sample selection criteria included married women lit in8 in ,seoul. Korea, between ages 30∼59. Purposive sampling method was used to secure respondents It with experience with imported formal two-piece suits. Of 400 distributed. 246 usable questionnaires were returned. Statistical analysis of results included factor analysis, and paired t-tests. For both imported and domestic suits, the most important buying motivation was utilitarian, with qualify the most important consideration. Purchases of domestic suits were more influenced by factors such as advertisements, economics, impulse buying, approval of others, and current fashionability of style than were the purchases of imported suits. On the other hand, purchases of imported suits u·ere more influenced by factors such as prestige and utilitarian motivation than were purchases of domestic suits. For most socioeconomic variables, respondents indicated significant differences in their motivations for buying imported suits and domestic suits. The results of this study suggest the need for implementing different marketing strategies for imported suits and domestic suits.

• Journal of the Korea Society of Computer and Information
• /
• v.6 no.3
• /
• pp.131-136
• /
• 2001

Men's dress shoe was made from Steer-finished leather manufactured by this study. Men's dress shoe applied by the cementing method in KS G 3116 and used by the chloroprene adhesive has been evaluated. For the qualify test of adhesive for Oxford using chloroprene adhesive, duration for environmental exposure was tested. Also, the quality test result of chloroprene adhesive, which had been applied to men's dress shoe as well as ladies' dress shoe, was better than expected. In case of adhesion-strength measurement. the KS G 3116 method of peel strength at the end of toe was found to be resonable compared to our measurement method through toe to heel seat on feather edge.