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Effect of N-Methylacetamide Concentration on the Fertility and Hatchability of Cryopreserved Ogye Rooster Semen (N-Methylacetamide 동결 보호제의 농도가 오계 동결 정액의 수정 및 부화율에 미치는 영향)

  • Kim, Sung Woo;Choi, Jin Seok;Ko, Yeoung-Gyu;Do, Yoon-Jung;Byun, Mijeong;Park, Soo-Bong;Seong, Hwan-Hoo;Kim, Chong-Dae
    • Korean Journal of Poultry Science
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    • v.41 no.1
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    • pp.21-27
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    • 2014
  • To preserve chicken genetic materials like cryopreserved spermatozoa, various kinds of freezing agents like glycerol, dimethylsuloxide, dimethylformamide or dimethylacetamide have been used for rooster semen preparation. Recently, the usage of N-methylacetamide (MA) for Ogye rooster semen preservation resulted in hatched chicken successfully. In this study, we investigated the effects of 7, 9 and 11% of MA on the viability, fertility and hatchability of frozen-thawed rooster semen using artificial insemination. The results of viability, fertility and hatchability in frozen semen with 7%, 9% or 11% MA were $35.16{\pm}6.12%$, $67.83{\pm}15.3%$ and $66.2{\pm}16.3%$ of motile sperm rate, 21.5%, 34.7% and 25% of fertility rate, and 100%, 89.5% and 87.5% of hatchability rate. The results of control group with frozen semen were 96.0% of fertility rate and 92.2% of hatchability rate. With these results, the concentration range of MA as a freezing agent of rooster semen could be 7~9% of media. The higher concentration of 9 % MA could decrease the fertility rate of thawed semen not the rate of hatchability rate. So the use of MA without affecting fertility rate would be a key point of freezing method of rooster semen for poultry genetic resource preservation.

Hospice & Palliative Care Policy in Korea (한국의 호스피스완화의료정책)

  • Kim, Chang Gon
    • Journal of Hospice and Palliative Care
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    • v.20 no.1
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    • pp.8-17
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    • 2017
  • Globally, efforts are being made to develop and strengthen a palliative care policy to support a comprehensive healthcare system. Korea has implemented a hospice and palliative care (HPC) policy as part of a cancer policy under the 10 year plan to conquer cancer and a comprehensive measure for national cancer management. A legal ground for the HPC policy was laid by the Cancer Control Act passed in 2003. Currently in the process is legislation of a law on the decision for life-sustaining treatment for HPC and terminally-ill patients. The relevant law has expanded the policy-affected disease group from terminal cancer to cancer, human immunodeficiency virus/acquired immune deficiency syndrome, chronic obstructive pulmonary disease and chronic liver disease/liver cirrhosis. Since 2015, the National Health Insurance (NHI) scheme reimburses for HPC with a combination of the daily fixed sum and the fee for service systems. By the provision type, the HPC is classified into hospitalization, consultation, and home-based treatment. Also in place is the system that designates, evaluates and supports facilities specializing in HPC, and such facilities are funded by the NHI fund and government subsidy. Also needed along with the legal system are consensus reached by people affected by the policy and more realistic fee levels for HPC. The public and private domains should also cooperate to set HPC standards, train professional caregivers, control quality and establish an evaluation system. A stable funding system should be prepared by utilizing the long-term care insurance fund and hospice care fund.

Estimation of value of freeway traffic information using Contingent Valuation Method (조건부가치측정법을 이용한 고속도로 교통정보의 가치 산정에 관한 연구)

  • Lee Eui-Eun;Kim Jun-jung
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.3 no.2 s.5
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    • pp.55-68
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    • 2004
  • On this research, Contingent Valuation Method which is universally applicable to non market goods and being developed in a field of the environmental economics is used to estimate appropriate value of freeway traffic information. Imaginary scenario is designed to estimate appropriate value of freeway traffic information and major in traffic-related field is used & man-to-man interview carried out to enhance a reliance of questionnaire. Open-ended question and a way of payment card are in use each other. make use of Multiple Regression Model in Contingent Valuation Method of traffic information and find an effect on the value of the traffic information. As a result of research, intention to pay for usage per information is regarded as a somewhat higher but monthly cost is lower than they have to. It is concluded that value and usefulness of the individual information is nod in approval but a majority of user's recognition that publish information must be provided free of charge lowers intention of payment.

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Study on Coagulant Application for Calcium Ammonium Nitrate Extraction of Denitrification Scrubber Waste Cleaning Solution (탈질 스크러버 폐 세정액에 포함된 질안석회 추출을 위한 응집제 적용 연구)

  • Lee, Hyun Suk;Song, Woon Ho
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.289-295
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    • 2017
  • The International Maritime Organization (IMO) in accordance with the regulations with respect to the combustion gases, such as NOx, SOx generated by the marine engine. The combustion gases must be equipped with a device to reduce emissions from all ships passing through the Baltic SECAs. In Korea, the International Maritime Organization (IMO) and the development of a device for NOx, SOx reduction. Scrubber is used in the ammonia water and the Urea solution in the waste water. The waste water containing ammonium nitrate and ammonium sulfate, react of the NOx and SOx gas. In this study, the recovery of by-product, which contains the waste water was used as an organic solvent extraction method of salting out. Ammonium nitrate and ammonium sulfate, the recovery process. A qualitative analysis of the collected by-product FT-IR analysis. Through the elemental analysis and SEM-EDS, characteristic evaluation was performed with an impurity.

고능력한우 유래의 수정란 생산 및 이식에 관한 연구

  • 한만희;최창용;정세환;김상희;신권희;권응기;최선호;손동수;이규승
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.144-144
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    • 2003
  • 본 연구는 수정란이식기술을 이용하여 혈통과 유전능력이 분석된 고능력한우로부터 수정란을 생산하고 이를 번식기반이 미약한 농가의 수란우에 이식함으로서 우수한우 유전자원을 조기확대 보급하여 농가단위의 개량 및 번식우 사육기반을 구축하기 위해 실시하였다. 공란우는 축산기술연구소 남원지소의 축군에서 유전능력이 평가된 우수암소를 선발하였고, 발정주기 9~11일째부터 Folltropin-V(Vetrepharm, Canada) 50mg씩을 4일간 12시간 간격으로 근육주사하고 투여 6회째에 dineprost (LutalyseTM, Upjohn, USA) 20mg을 근육주사하여 과배란을 유기하였다. 인공수정은 dinoprost 주사후 48시간 전후에 발정을 확인하고 12시간 간격으로 2straw씩의 정액으로 3회 실시하였으며, 2차 인공수정 후 100$\mu\textrm{g}$ GnRH를 근육주사하였다. 수정란 채란은 공란우의 발정확인후 7~8일째에 balloon catheter(FHK, Japan)를 이용하여 비외과적방법으로 수정란을 회수하였다. 수란우는 각기 다른 사육조건의 4개 농가(OB, BD, SH 및 SG농장)에서 양호한 번식성적을 가진 개체를 10두씩 선발하여 CIDR plus(EAZI-Breed, New-Zealand)를 질내 7일간 삽입하고, CIDR plus제거 1일전에 dinoprost 20mg을 근육주사하여 발정동기화를 유도하였다. 수란우의 발정상태가 정상이며 발정주기 7~8일째에 직장검사법으로 황체검사를 실시하여 황체가 존재하는 쪽의 자궁각에 수정란 1 개를 이식하였다. 수정란이식 후 13일째에 혈액을 채취하여 임신진단키트(제네디아프로테 트, 녹십자)를 이용하여 임신여부를 1차적으로 확인하였다. 과배란을 유기한 13두의 공란우중 9두(69.2%)가 과배란 반응을 나타내었으며, 회수된 수정란 51개중 이식가능수정란은 38개(74.5%) 였다. 발정동기화를 유도한 수란우 40두중에서 35두(87.5%)가 발정이 동기화되었으며, 그 중 황체검사를 통하여 30두의 수란우에 수정란을 이식하였다. 수정란이식후 13일(발정주기 21일)에 혈액을 이용한 임신진단에서 농가별 수태율은 각각 37.5%, 70.0%, 60.0% 및 71.4% 로서 평균 60.0%를 나타내었다.

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Analysis of Withdrawal Strategies in Retirement Assets Reflecting Risk Aversion Based on Programmed Withdrawal (위험회피성향을 반영한 퇴직자산 지급방식 분석에 관한 연구 - Programmed Withdrawal 중심으로)

  • Yeo, Jeong-Mi;Kang, Jung-Chul;Sung, Joo-Ho
    • Communications for Statistical Applications and Methods
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    • v.17 no.5
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    • pp.653-666
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    • 2010
  • Under the retirement pension plan enforced since December 2005, retirees can just choose the payout strategy either of a lump sum allowance or of an annuity in receiving the retirement benefit. Therefore, it is imperative to review and introduce the program withdrawal system enforced by countries with mature pension plan, and complement the limitations of the current payout strategy in the future. In this study, the appropriateness of each of the payout strategies related to the program withdrawal system is examined in terms of shortfall risk and bequest fund per each risk propensity through the expected utility model that reflects the age of the retiree.

Cryopreservation of Recipient Oocytes Collected from Korean Native Cattle: Incidence of the Zona Hardening of Recipient Oocytes Collected from Korean Native Cattle at the Different Stages of Cryopreservation (복제 한우 생산을 위한 수핵난자의 동결에 관한 연구 : 상이한 동결과정 중 한우 수핵난자에서 일어나는 투명대 경화)

  • 이병천;박종임;임정묵;이은송;노상호;황우석
    • Journal of Embryo Transfer
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    • v.14 no.2
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    • pp.99-106
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    • 1999
  • 핵 이식에 공여되는 수핵난자의 효과적인 동결보존을 위하여 한우 성숙난자를 1.0 M dimethylsulfoxide(DMSO) 또는 1.0 M glycerol이 함유된 동결보호제를 이용하여 처리하거나, 동결보호제 처리 후 완만동결법을 이용한 동결융핼르 시행하여 상기 실험처리로 야기되는 투명대 경화현상을 관찰하였다. 도축장 유래의 난소에서 미성숙난자를 채취한 후 10% 소 태아혈청을 함유한 TCM-199을 이용하여 22∼24 시간 동안 체외성숙배양을 이해하였다. 배양후 작출된 성숙난자를 각각의 동결보호제로 처리, 혹은 처리 후 동격융해한 후 protease를 이용하여 투명 대의 경화현상 발생의 빈도를 조사하였다. 또한 동격란을 동결정액을 이용한 체외수정에 공여한 후 정자 침입농도능을 조사하였다. 동결보호제로 처리한 난자에 있어서 보호제의 종류와 관계없이 투명대 경화현상이 유의적 (P<0.05) 으로 증가하였으나 이후의 동결융해 처리에 의한 추가적인 경화현상의 발생은 증가하지는 않았다. 또한 투명대 경화현상의 발생양상을 동결보호제 처리 후 10분 간격으로 측정한 결과 DMSO의 경우 처리후 10분, glycerol의 경우 처리 후 20분 후부터 유의적으로 차를 발견할 수 없었으며, 수정율 및 난자 1개당 침입한 정자의 수는 동결란에서 유의적으로 증가하지 않았다. 본 연구의 결과 동결난자의 투명대 경화현상은 동결보호제 처리과정에서 이미 일어나지만, 이러한 투명대 경화현상이 난자의 동결보존 후 수정능에는 현자한 영향을 미치지 않는다는 사실이 규명되었다.

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Optimal Method for Fertilization of Surf Clam, Spisula sachalinensis (북방대합, Spisula sachalinensis 수정을 위한 최적방법)

  • Lee Jeong Yong;Chang Yun Jeong;Chun Min Jee;Chang Hae Jin;Chang Young Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.2
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    • pp.135-139
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    • 2002
  • In order to obtain the basic information for the seedling production of surf clam, Spisula sachalinensis, sperm motility and optimal method for fertilization were investigated. Sperm concentration of S. sachalinensis milt was$ 2.02{\times}10^{10}\;cell/mL$ and approximately $96.0\%$ of sperm showed forward movement after exposure to seawater. When sperm and eggs obtained by incision method were fertilized in 1 hour and 4 hours, respectively, high fertilization and hatching rate were achieved. The optimal sperm concentrations and egg density for fertilization and hatching were 10$\~$100 inds./egg and 100$\~$200 inds./mL sea water, respectively.

A Study on the Unified Operation and Maintenance System of Irrigation Facilities in Korea - With Reference to the Survey Results on the Activities of Irrigation Fraternities in Chungnam Province - (농업용수리시설의 유지관리체제일원화에 관한 연구 - 충남지역 수리계 운영실태조사결과를 중심으로 -)

  • Kim, Sung-Min;Lim, Jae-Hwan
    • Korean Journal of Agricultural Science
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    • v.31 no.1
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    • pp.53-75
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    • 2004
  • This study is aimed at identifying the national economic value of the irrigation facilities by reviewing the existing papers on economic values of the irrigation facilities and presenting current status of dual O & M problems of the irrigation facilities. This study suggested the unified O & M system rather than continuing the existing dual O & M system of irrigation facilities based on the surveyed results of the activities of irrigation fraternities in Chungnam Province. The findings and proposals for the successful unified and mono O & M system of the irrigation facilities are as follows: (1) Total number of irrigation facilities in the nation accounts for 67,582, while the total length of irrigation and drainage canals amounted to about 174,259km. On account of the total length of structural canals was estimated at 31%, much losses of water and much O & M costs have been inevitable for the full irrigation rice culture. In spite of the past heavy investment for irrigation facilities, the ratio of rain-fed and partially irrigated paddy fields accounts for 23% in 2003. Both Korea Agricultural and Rural Infrastructure Corporation (KARICO) and the city and Gun Governments have managed the irrigation facilities separately by irrigation fraternities. The KARICO have commanded 59% of irrigation paddy area with 18% of the total irrigation facilities, while the city and Gun governments covered 41% of irrigation paddy area with 82% of the existing number of irrigation facilities representing small and medium scale. (2) The 1999 demand prices of irrigation water per ton expressed in 2000 constant market price was estimated at 388 won, the supply price was amounted to 184 won per ton. Considering the supply and demand curve of the irrigation water, the existing irrigation facilities could not satisfy the demand of irrigation water. (3) In 1999, total present added value of the irrigation facilities during the economic life accounted for 48 trillion won, while total supply cost was 44.7 trillion won. The marginal benefit and cost ratio of irrigation water was 1.08. (4) The total O & M cost per year amounting to 681.1 billion won have been required to maintain and repair the existing irrigation facilities in Korea. For the successful unified O & M of irrigation facilities covering whole irrigated paddy field in Korea, 950 billion won of O & M costs are required to keep up the marginal benefit of irrigation water as 2,800 billion won per year. The total O & M cost as 950 billion won should be allocated 40%, 380 billion won for O & M costs of irrigation facilities and 60%, 570 billion won for improvement of irrigation facilities. (5) The study investigated and reviewed the present O & M status of the irrigation facilities by small and medium irrigation fraternities. Most of the farmers belong to the irrigation fraternities preferred not only unified O & M but also KARICO take-over of the whole O & M activities of the irrigation facilities. The prevailing O & M cost per 10a expended by the Corporation was amounted to 104,890 won, while that of city and Gun governments was only amounted to 4,600 won per 10a. regarding the small amount of O & M cost expended by city and Gun governments, it is evident that the existing irrigation system have been managed ineffectively and deteriorated the facilities comparing that of KARICO. In conclusion, the Government could not satisfied the demand of irrigation water by suppling water with existing irrigation facilities. Therefore new additional investment and financial support for irrigation water development should be made to convert rain-fed and partially irrigated paddy fields into fully irrigated ones. The operation and maintenance cost should be supported to keep the marginal values of rice production of existing irrigation facilities in the national economy and to modernize the obsolete irrigation facilities. By unifying the existing dual O & M systems, all the farmers belong to the irrigated paddy fields have to be equally benefited and could be increased their farm income and be stabilized their rural lives.

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A Practical Approach to Implement QoS in Broadband Access Networks (광대역 접속망에서 QoS 구현을 위한 현실적 접근법)

  • Park Seung-Chul
    • Journal of KIISE:Information Networking
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    • v.33 no.3
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    • pp.277-287
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    • 2006
  • Most of the existing broadband access networks based on DSL, cable modem, and Ethernet support the best-effort internet access service, and adopt the flat rate pricing mechanism. It is almost impossible to provide the differentiated communication services, in current broadband access networks, for the different users and/or the different application services. Currently, however, the advances in multimedia, communication, and security technologies push the interactive and/or streaming multimedia services and VPN services to be widely deployed over Internet, and they require more QoS-sensitive services than the best-effort service. Though various QoS technologies such as RSVP-based IntServ and DiffSern were already developed and under standardization in Internet world, it is impractical to replace the existing QoS-unaware access networks with the QoS-enabled ones at a time to deploy QoS-sensitive services. In this paper, after analyzing current broadband access network architectures and the status of QoS support, we propose a practical approach to support multimedia QoS in the broadband access networks. The approach will be based on the integration of the differentiated pricing and the DiffServ technology. And it will be a step-wise approach to support backward compatibility with the legacy broadband access networks as much as possible.