• Korean Journal of Environmental Agriculture
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• v.37 no.2
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• pp.117-124
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• 2018

BACKGROUND: Oxycarboxin(5,6-dihydro-2-methyl-N-phenyl-1,4-oxathiin-3-carboxamide-4,4-dioxide) as oxanthiin is a systemic fungicide commonly used for control of various pathogens in agronomic and horticultural crops. In an effort to develop an analytical method to trace the fungicide, a method using HPLC equipped with UVD/MS was studied. METHODS AND RESULTS: Oxycarboxin was extracted with acetone from hulled rice, soybean, Kimchi cabbage, green pepper, and apple samples. The extract was diluted with saline water, followed by liquid-liquid extraction with methylene chloride. Florisil column chromatography was employed for the purification of the extracts. Oxycarboxin was determined on a Zorbax SB-AQ $C_{18}$ column by HPLC with UVD. Accuracy of the proposed method was validated by the recovery tests from crop samples fortified with oxycarboxin at 3 levels per crop. CONCLUSION: Mean recoveries ranged from 78.3% to 96.1% in five representative agricultural commodities. The coefficients of variation were less than 10%, and limit of quantitation of oxycarboxin was 0.04 mg/kg. A confirmatory technique using LC/MS with selected-ion monitoring was also provided to clearly identify the suspected residue. The method was reproducible and sensitive to determine the residue of oxycarboxin in agricultural commodities.

• Proceedings of the Korean Nuclear Society Conference
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• 1997.10a
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• pp.765-771
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• 1997

인간신뢰도분석 절차인 SHARP(Systematic Human Action Reliability Procedure)와 인간행위 정량화 방법인 THERP(Technique for Human Error Rate Prediction)를 토대로 하고 원자력발전소의 저출력/정지 운전의 특징적인 상황을 반영하여 가압 경수로의 저출력/정지운전의 PSA를 위한 인간신뢰도분석 절차서를 개발하였다. 개발된 인간신뢰도분석 절차서의 주요사항은 다음과 같다; 1) 원자력발전소의 이상사태에 대응하는 운전원 행위는 두 개의 기본사건인 진단실패와 수행실패 사건으로 모델링 한다. 2) 절차서에 없는 행위이라도 일부 운전원이 그 행위에 대한 절차와 조건을 알고 있으면 그 행위에 대해 성공가능성을 고려한다. 3) 인간신뢰도분석시 본 연구에서 개발된 표(work sheet)의 사용으로 인간행위 정량화 과정에 대한 타당성 및 신뢰성을 제고시키고 정량화과정을 쉽게 추적할 수 있다. 4) 인간신뢰도분석자의 판단이 필요한 부분에 결정수목을 사용하기 때문에 인간신뢰도 분석 시 개입될 수 있는 분석자의 주관성을 일정부분 배제할 수 있고 일관된 인간신뢰도분석을 수행 할 수 있다.

• Journal of Applied Biological Chemistry
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• v.63 no.2
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• pp.153-159
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• 2020

The analytical method was established for simultaneous determination of fungicide prochloraz and its metabolites in several animal commodities using gas chromatography (GC) coupled with electron capture detector (ECD). Samples including beef meat, pork meat, chicken meat, milk, and egg were hydrolyzed with pyridine hydrochloride which converts prochloraz and its metabolites to 2,4,6-trichlorophenol (2,4,6-TCP) because residue definition for prochloraz was 'sum of prochloraz and its metabolites containing the 2,4,6-trichlorophenol moiety, expressed as prochloraz', for compliance with MRLs from animal commodities. Therefore, residual prochloraz was extracted with acetone, decomposed to 2,4,6-TCP, partitioned with dichloromethane, purified with aminopropyl SPE and quantified as 2,4,6-TCP with GC-ECD. The instrumental limit of quantitation and method LOQ (MLOQ) was 0.01 ㎍/mL and 0.02 mg/kg for prochloraz and 0.005 ㎍/mL and 0.01 mg/kg for 2,4,6-TCP, respectively. The linearity of the calibration curve was good with R² >0.995 in the range of 0.005-0.2 ㎍/mL. Fortification levels of prochloraz were 0.02 mg/kg (MLOQ) and 0.2 mg/kg (10MLOQ) for recovery tests. Overall recoveries of prochloraz were >90% with <10% of coefficient variation (C.V.). This established analytical method was fully validated and could be useful for quantification of prochloraz and its metabolites in animal commodities as official analytical method.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.6
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• pp.631-638
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• 2015

In this study, semiquantitative failure mode, effects, and criticality analysis (FMECA) for the reliability analysis of a solid rocket propulsion system is performed. The semiquantitative FMECA is composed of failure mode and effects analysis (FMEA) and criticality analysis (CA). To perform FMECA, the structure of the solid rocket propulsion system is divided into 43 parts down to the component level, and FMEA is conducted at the design stage considering 137 potential failure modes. CA is then conducted for each failure mode, during which the criticality number is estimated using the failure rate databases. The results demonstrate the relationship between potential failure modes, causes, and effects, and their risk priorities are evaluated qualitatively. Additionally, several failure modes with higher criticality and severity values are selected for high-priority improvement.

• Korean Journal of Construction Engineering and Management
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• v.8 no.4
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• pp.99-109
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• 2007

Risk elements of highway safety facilities are affected by complex environments. Thus, risk-based approach for traffic safety facilities is needed. For this, in this study, qualitative and quantitative risk assessment methodology and procedure for highway safety facilities is proposed, which can be used as risk-based approach incorporating VE process. Also, for the quantitative risk assessment, event tree using EPDO(Equivalent Property Damage Only) with respect to frequency and magnitude of risk events is introduced. As a result, risk index of alternative 1(140cm) and 2(127(cm) which can be used as performance factor in VE approach are estimated.

• Journal of Ginseng Research
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• v.1 no.1
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• pp.79-88
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• 1976

A determinatien of the saponins in ginseng tea and extract was carried out by using the quantitative TLC autodetector equipped with a hydrogen flame ionization detector. In order to apply to the Quality central of the ginseng tea and extract. the optimum condition and recovery percentage for the quantitative determination of saponins in these products duo studied. The results obtained were as follows: The method was adequate to estimate whether the ginseng extract used for the Products and She raw ginseng extract were the same quality or net. Most of the individual peak area was increased with the concentration of the total saponin. But some of the peak areas were net increased quantitatively in the ease of the sample containing high concentration ginseng extract. To deternine the saponins in ginseng tea correctly high volume low concentration was better than the low volume high concentration. Optimum concentration of ginseng extract in sample to determine the individual saponins was in the range of 0.5∼1.5g. The recovery percentage of the total saponin was 99.5% on the average.

• Journal of Society of Preventive Korean Medicine
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• v.2 no.1
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• pp.13-30
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• 1998

Geraniol (1), olivetol (2), cannabinoids (3 and 4) and 5-fluorou.a.il (5). were tested for their growth inhibitory effects against SK-MEL-3 cell lines using two different 3-(4,5-dimethylthiazol- 2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and sulforhodamine B protein (SRB) assay. These compounds showed inhibitory activity in vitro in the micromolar range against SK-MEL-3 cell lines. In general, the antitumor activity of these compounds (1, 2, 3, 4 and 5) was in a dose-dependent over the micromolar concentration range $1\;to\;100{\mu}M$. The comparison of $IC_{50}$ values of these compounds in tumor cell lines shows that their susceptibility to these compounds decrease in the following order : OLVTL > CBG > CBD > 5-FU > CRNL in MTT assay, CBG > OLVTL > CBD > GRNL > 5-FU in SRB assay. Cannabinoids (3 and 4) and 5-fluorouracil (5) were tested for their cytotoxic effects on NIH 373 fibroblasts using two different MTT assay and SRB assay. These compounds exhibited potent cytotoxic activities in vitro in the micromolar range against NIH 373 fibroblasts. In general, the cytotoxic activities of these compounds (3, 4 and 5) were in a dose-dependent over the micromolar concentration range $1\;to\;100{\mu}M$. The comparison of $CD_{50}$ values of these compounds on NIH 373 fibroblasts shows that their susceptibility to these compounds decrease on the following order ; CBD > 5-FU > CBG in MTT assay and SRB assay. Cannabigerol (3) was shown the least cytotoxic activity on NIH 373 fibroblasts. Cannabigerol (3) exhibited the most growth-inhibitory activity against SK-MEL-3 cell lines.

• Analytical Science and Technology
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• v.29 no.5
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• pp.234-241
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• 2016

The approach presented in this article refers to the bioanalytical method validation for the detection and quantitative determination of arsenic species including arsenite (As(III)), arsenate (As(V)), dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) in dog plasma by high-performance liquid chromatography inductively coupled plasma mass spectrometry (HPLC-ICP/MS). The arsenic species were separated using an agilent As speciation column by a mobile phase of 2 mM sodium phosphate monobasic, 0.2 mM ethylenediaminetetraacetic acid disodium salt dehydrate, 10 mM sodium acetate, 3 mM sodium nitrate and 1 % ethyl alcohol at pH 11 (adjusted with 1M NaOH). The method validation experiment was obtained selectivity, linearity, accuracy, precision, matrix effect, recovery, system suitability, dilution integrity and various stabilities. All calibration curves showed good linearity (R²>0.999) within test ranges. The lower limit of quantitation (LLOQ) was 5 ng/mL for As(III), As(V) and DMA, and 20 ng/mL for MMA. The system suitability and dilution values were within 6.5 % and 7.7 %. Subsequently, the developed and validated HPLC-ICP/MS method was also successfully applied to determine the arsenic speciation in dog plasma samples, and the recoveries for the spiked samples were in the range of 91.5–102.2 %. Therefore, this method could be applied to the evaluation of arsenic exposure, health effect assessment and other bio-monitoring studies in biological samples.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.451-458
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• 2009

Purpose: Optical molecular luminescence imaging is widely used for detection and imaging of bio-photons emitted by luminescent luciferase activation. The measured photons in this method provide the degree of molecular alteration or cell numbers with the advantage of high signal-to-noise ratio. To extract useful information from the measured results, the analysis based on a proper quantification method is necessary. In this research, we propose a quantification method presenting linear response of measured light signal to measurement time. Materials and Methods: We detected the luminescence signal by using lab-made optical imaging equipment of animal light imaging system (ALIS) and different two kinds of light sources. One is three bacterial light-emitting sources containing different number of bacteria. The other is three different non-bacterial light sources emitting very weak light. By using the concept of the candela and the flux, we could derive simplified linear quantification formula. After experimentally measuring light intensity, the data was processed with the proposed quantification function. Results: We could obtain linear response of photon counts to measurement time by applying the pre-determined quantification function. The ratio of the re-calculated photon counts and measurement time present a constant value although different light source was applied. Conclusion: The quantification function for linear response could be applicable to the standard quantification process. The proposed method could be used for the exact quantitative analysis in various light imaging equipments with presenting linear response behavior of constant light emitting sources to measurement time.

• Proceedings of the Korean Society for Rock Mechanics Conference
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• 2002.10a
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• pp.31-42
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• 2002

최근 지하철 터널은 사용자 편의성, 도심지 접근성 및 원활한 교통처리 등을 고려하여 지반조건이 불리한 상황에서도 터널로 계획되는 경우가 많아지고 있다 따라서 시공중의 터널안정성확보, 굴착에 따른 인접구조물의 침하영향, 발파진동영향 등을 종합적으로 고려한 지보패턴 및 보조·보강공법의 결정이 매우 중요하나 정량적인 판단기준의 부재로 인하여 주로 경험적인 설계에 의존하는 경우가 많다. 본 연구에서는 도심지 지하철 터널의 복합적인 거동특성을 고려하기 위하여 여러 가지 예상위험요소의 정량화 방안을 제안하고, 다변량 통계분석기법을 활용하여 여러 가지 위험 요소들의 특성을 함축적으로 나타내는 소수의 총합적인 지표(안정성인자, 환경성인자)로 대표화 할 수 있음을 검증하였다. 안정성 인자 및 환경성 인자를 이용한 서울시 지하철 00공구 설계사례를 통해 정량화지표(Multiple Index)의 터널설계에의 적용성을 평가하고 이의 설계시 활용방안을 제안하고자 하였다.