• Title/Summary/Keyword: 정량 광 유도 형광법

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A Planar Imaging System for Spray Patternation (분무 패턴 측정을 위한 평면 이미지 기법)

  • 이경진;정기훈;윤영빈;정경석;정인석
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 1998.10a
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    • pp.7-7
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    • 1998
  • 액체 연료 분포의 균일성과 대칭성은 연소 효율을 높이고, 공해 물질을 줄이는데 있어서 필수적인 요소이기 때문에 분무 패턴을 정량적으로 해석하는 것은 매우 중요하다. 고전적으로 사용되고 있는 mechanical patternator는 ⅰ) 유동을 교란시키고, ⅱ) 공간 분해능이 떨어지며, ⅲ) 복잡한 재순환 유동이나 화학 반응이 있는 유동에서의 적용이 어렵다는 단점을 가지고 있다. 이러한 단점들을 극복하기 위하여 레이저를 이용한 optical patternator에 대한 연구가 진행되고 있다. Optical patternator는 유동을 교란시키지 않으면서 고해상도의 분무 패턴 측정을 할 수 있다. 형광 신호는 물질의 체적에 비례한다. 따라서, 레이저 평면광을 분무에 조사시켜 주어서 유도되는 형광 신호를 이용하는 평면액체 레이저 유도 형광법(PLLIF : Planar Liquid Laser Induced Fluorescence)은 분무패턴을 알아보는 매우 유용한 방법이다.

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The Determination of Rare Earth Oxides by X-Ray Fluorescence Spectrometry Using Empirical Coefficient Method (실험계수법을 이용한 희토류산화물의 X-선 형광분광분석)

  • Young Man Kim;Beom Suk Choi;Sun Tae Kim;Chong Wook Lee
    • Journal of the Korean Chemical Society
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    • v.31 no.1
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    • pp.64-70
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    • 1987
  • Rare earth elements including Y and Th in the monazite were separated and determined by X-ray fluorescence spectrometry. The matrix effects among the rare earth elements were simultaneously corrected by means of empirical coefficient method. The values of the coefficients were quite dependent on the number of the standards. However, the different set of coefficients led to the same results. The analytical results corrected by the present method agreed with those by the inductively coupled plasma spectrometry.

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Diagnosis of Early Dental Caries with Dye-Enhancing Quantitative Light-Induced Fluorescence (QLF) (정량 광유도 형광법(QLF)과 광활성제를 이용한 초기 치아우식증의 진단)

  • Kim, Mihee;Lee, Sangho;Lee, Nanyoung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.3
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    • pp.218-225
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    • 2015
  • This study used sodium fluorescein to improve imaging diagnostic ability by increasing the fluorescence difference between sound enamel and caries lesions. It also made it easier to discriminate between stain and caries lesions using quantitative light-induced fluorescence (QLF). Half of the specimen surface was covered with nail varnish as a control. Specimens were divided randomly in six decalcification groups and decalcified for different lengths of time. Then, ${\Delta}F$ was measured using QLF-D. After applying 0.075% sodium fluorescein, we measured ${\Delta}F$ again and compared it with the initial value. After cutting the central portion of the specimen, we measured the lesion depth using scanning electron microscopy. The lesion surfaces observed with QLF were darker than normal enamel, whereas they were lighter than normal enamel after applying fluorescein. Longer decalcification time was associated with greater fluorescent dye penetration. The ${\Delta}F$ measured after applying fluorescein was higher than the initial value (p < 0.05). Due to QLF measurement using fluorescein being more sensitive for diagnosing early decalcification, this approach will enable early diagnosis of dental caries before the cavity formation stage, allowing the treatment of early caries lesions. With QLF and sodium fluorescein, we can easily discriminate between stain and caries lesions.

Oil Fluorescence Spectrum Analysis for the Design of Fluorimeter (형광 광도계 설계인자 도출을 위한 기름의 형광 스펙트럼 분석)

  • Oh, Sangwoo;Seo, Dongmin;Ann, Kiyoung;Kim, Jaewoo;Lee, Moonjin;Chun, Taebyung;Seo, Sungkyu
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.18 no.4
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    • pp.304-309
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    • 2015
  • To evaluate the degree of contamination caused by oil spill accident in the sea, the in-situ sensors which are based on the scientific method are needed in the real site. The sensors which are based on the fluorescence detection theory can provide the useful data, such as the concentration of oil. However these kinds of sensors commonly are composed of the ultraviolet (UV) light source such as UV mercury lamp, the multiple excitation/emission filters and the optical sensor which is mainly photomultiplier tube (PMT) type. Therefore, the size of the total sensing platform is large not suitable to be handled in the oil spill field and also the total price of it is extremely expensive. To overcome these drawbacks, we designed the fluorimeter for the oil spill detection which has compact size and cost effectiveness. Before the detail design process, we conducted the experiments to measure the excitation and emission spectrum of oils using five different kinds of crude oils and three different kinds of processed oils. And the fluorescence spectrometer were used to analyze the excitation and emission spectrum of oil samples. We have compared the spectrum results and drawn the each common spectrum regions of excitation and emission. In the experiments, we can see that the average gap between maximum excitation and emission peak wavelengths is near 50 nm for the every case. In the experiment which were fixed by the excitation wavelength of 365 nm and 405 nm, we can find out that the intensity of emission was weaker than that of 280 nm and 325 nm. So, if the light sources having the wavelength of 365 nm or 405 nm are used in the design process of fluorimeter, the optical sensor needs to have the sensitivity which can cover the weak light intensity. Through the results which were derived by the experiment, we can define the important factors which can be useful to select the effective wavelengths of light source, photo detector and filters.

Evaluation of Remineralization Effects on Enamel Demineralization by Anti-cariogenic Agents using Quantitative Light-induced Fluorescence-digital (QLF-D) in vitro (유치 및 영구치에서 QLF를 이용한 항우식 제품의 재광화 효과 비교)

  • Lee, Kkotnim;Kim, Miae;Hwang, Inkyung;Park, Jihyun;Mah, Yonjoo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.43 no.4
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    • pp.391-400
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    • 2016
  • The purpose of this study was to evaluate the difference of remineralization effects of various anti-cariogenic toothpastes on artificial carious lesions in primary and permanent teeth using quantitative light-induced fluorescence-digital (QLF-D) system. Sound human primary (n = 48) and permanent teeth (n = 48) were randomly divided into following groups : control group (Group 1), fluoride toothpaste (Group 2), functionalized tricalcium phosphate (fTCP) + fluoride toothpaste (Group 3), and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) toothpaste (Group 4). Specimens were prepared by exposure in a demineralizing solution and then treated using the different toothpastes twice daily during 14 days. All specimens were analyzed with the QLF-D system. QLF data analysis indicated three different toothpastes showed significant remineralizing effects compared to Group 1 in both primary and permanent teeth. Also, the remineralizing effects in Group 3 and 4 were significantly higher than in Group 2. This study suggested that the toothpastes containing fTCP + fluoride and CPP-ACP have the significant anti-cariogenic effects on enamel demineralization in both primary and permanent teeth, and QLF-D is an useful device to assess the incipient carious lesion and remineralization effects of the anti-cariogenic materials quantitatively. Therefore, clinicians can consider the QLF-D system for the evaluation of demineralization and remineralization in primary and permanent teeth.

Anti-aging Effects of L-Carnitine on Human Skin (L-카르니틴의 사람피부에 대한 항노화 효과)

  • Lee Bum-Chun;Choe Tae-Boo;Sim Gwan-Sub;Lee Geun-Soo;Park Sung-Min;Lee Chun-Il;Pyo Hyeong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.3 s.47
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    • pp.393-397
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    • 2004
  • L-Carnitine $({\beta}-hydroxy-{\gamma}-trimethyl-ammoniumbutyric{\;}acid)$ is a small water-soluble molecule important in mammalian fat metabolism. It is essential for the normal oxidation of fatty acids by the mitochondria, and is involved in the trans-esterification and excretion of acyl-CoA esters. In this paper, to investigate the relationship between aging and L-carnitine, we investigated the effects of in vitro matrix-metalloproteinase (MMP) inhibition and activity and expression of UYA-induced MMPs in human skin fibroblasts. Also, we studied to develop as anti-aging cosmetics with L-carnitine. Fluorometric assays of the proteolytic activities of MMP-1 (collagenase) were performed using fluorescent collagen substrates. ELISA (enzyme linked immune sorbent assay), gelatin-substrate zymography, RT-PCR ELISA techniques were used for the effects of L-carnitine on MMP expression, activity, and MMP mRNA expression in UVA irradiated fibroblast $(5\;J/cm^2)$, respectively. In addition, we performed clinical study with L-carnitine cream. L-carnitine inhibited the activities of MMP-1 in a dose-dependent manner and the $IC_{50}$ values calculated from semi-log plots were 2.45 mM, and L-carnitine showed strong inhibition on MMP-2 (gelatinase) activity in UVA irradiated fibroblast by zymography. Also, UVA induced MMP-1, 2 expression was reduced $43\%,\;53\%$ by treated with L-carnitine at 1.25 mM, and MMP-1 mRNA expression was reduced dose-dependent manner. Therefore L-carnitine was able to significantly inhibit the MMP activity, and regulate MMP expression in protein and mRNA level. The results of clinical study showed that $1.0\%$ L-carnitine treated group reduced wrinkle significantly compared with placebo treated group (P<0.05). All these results suggest that L-carnitine may be useful as new anti-aging cosmetics for protection against UVA induced Mm expression and activity.