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Isolation and Characterization of Lactobacillus brevis AML15 Producing γ-Aminobutyric acid ((γ-Aminobutyric acid를 생산하는 Lactobacillus brevis AML15의 분리 및 특성)

  • Shin, Ji-Won;Kim, Dong-Geol;Lee, Yong-Woo;Lee, Hyoung-Seok;Shin, Kee-Sun;Choi, Chung-Sig;Kwon, Gi-Seok
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.970-975
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    • 2007
  • For the screening of ${\gamma}-aminobutyric$ acid (CABA)-producing bacteria, 86 bacterial strains which produce GABA were isolated from Kimchi and Salted fisk .Among these, three strains designated AML15, AML45-1, AML72 with relatively high GABA productivity were selecled by thin layer chromatography (TLC). To elucidate the relationship between isolated strains and the genus Lactobacillus, their 16S rDNA sequence were examined. The result of their DNA sequences showed 99% similarity with Lactobacillus brevis ATCC 367. On the basis of the these results, isolated strains were identified as Lactobacillus brevis and designated L. brevis AML15. In order to determine the optimum conditions for GABA production, the isolated strains were cultivated in pyridoxal phosphate (PLP) and monosodium glutami. acid (MSG). Results showed that L. brevis AML15 had the highest CABA productivity with 10,424 $nM/{\mu}l$ concentration in MRS broth containing 5% (w/v) MSG and 10 ${\mu}M$ PLP at pH 5.0. The results imply that L. brevis AML15 has the potential to be developed as a strain for GABA hyper-production.

Na-binding Capacity of Alginate and Development of Sea Tangle Added Kimchi (Alginate의 Na 흡착효과와 다시마 첨가 김치의 개발)

  • 하정옥;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.6
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    • pp.995-1002
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    • 2000
  • In order to Develop a low Na functional kimchi using sea tangle, the Na-binding capacity of alginate in sea tangle along with other dietary fibers was evaluated in vitro. The adding type and amount of the sea tangle that contains alginate in kimhi and characteristics of the sea tangle added kimchi were also studied. Na-binding capacity of various dietary fibers such as cellulose, pectin, gun gum, carageenan, alginates (sodium alginate, alginate, alginate from sea tangle) was measured by equilibrium dialysis method in pH 2 and pH 7 in vitro. Gua gum, carageenan and a group of alginates effectively bound to Na+ Espacially sodium alginate showed high Na-binding capacity of 29.2% in pH of stomach (pH 2.0) and 33.8% in pH of small intestine (pH 7.0), however, the alginate extracted from sea tangle could not bind Na in PH of stomach (pH 2.0), but 27.4% in pH of small intestine (pH 7.0) condition. The content of alginate in sea tangles (dried sea tangle, salted sea tangle and washed salted sea tangle) was 19.8 ~ 22.2% on dry matter basis. The sea tangle added kimchi was prepared with the addition of the flake type (0.5$\times$3 cm) of sea tangle with a quantity of 30% in kimchi from the data of the sensory analysis. The addition of the sea tangle to the kimchi increased the content of soluble dietary fiber, suggesting the Na-binding capacity increased. The sea tangle added kimchi (SK) and sea tangle and fermented anchovy added kimchi (SAK) showed higher levels of reducing sugar and acidity than the control kimchi (CK). In quantitative descriptive analysis (QDA) SK and SAK showed higher score in overall acceptance, and lower score in acidic order than CK, however, SK showed less moldy taste and more fresh acidic taste than SAK.

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Studies on the Lipid Content and Fatty Acid Composition of Anchovy Sauce Prepared by Heating Fermentation (멸치액젓의 가온숙성중 지질함량 및 지방산 조성의 변화)

  • KIM Dong-Soo;KOIZUMI Chiaki;JEONG Bo-Young;JO Kil-Suk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.5
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    • pp.469-475
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    • 1994
  • Lipid content and fatty acids composition of anchovy sauce were investigated during fermentation at $20^{\circ}C\;and\;37^{\circ}C$ for 120 days. Total lipid(TL) of raw anchovy was composed of $80.8\%$(1.98g/100g) of non-polar lipid(NL), $19.2\%$ (0.47g/100g) of phospholipid(PL) The contents of TL, NL and PL in the anchovy sauce decreased during fermentation, but the ratio of NL/TL increased from $81.1\%\;to\;90.0\%$ while that of PL/TL decreased from $18.9\%\;to\;10.0\%$. The major fatty acids in TL and NL fractions of fermented anchovy sauce were 16:0, 18:0, 18:1n-9, 16:1n-7, 18:1n-7, 20:5n-3 and 22:6n-3, 22:6n-3 was highest in the PL fraction regardless of temperature or fermentation time.

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The Shelf-Life Extension of Low-Salted Myungran-Jeot 1. The Effects of pH Control on the Shelf-life of Low-Salted Myungran-Jeot (저염 명란젓의 Shelf-Life 연장 방안 1. pH 조정에 의한 연장 효과)

  • KIM Sang-Moo;LEE Keun-Tai
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.459-465
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    • 1997
  • In order to extend the shelf-life of the low-salted Myungran-Jeot, before immersing alaska pollack roe, the salt solutions was adjusted to pH 7.0 and 4.7, respectively. pH during ripening was somewhat constant, whereas the content of lactic acid was slightly increased, and especially, that of pH 4.7 Myungran-Jeot was increased rapidly in the begining of fermentation period. $NH_2-N$ content of pH 4.7 Myungran-Jeot was increased with increasing fermentation period, whereas those of control and pH 7.0 decreased except the begining of fermentation period. The contents of TMA, TBA, and VBN of pH 4.7 were lower than those of control and pH 7.0. In addition, the mirrobial growth was significantly inhibited in pH 4.7 Myungran-Jeot. The estimated shelflives of control, pH 7.0, and pH 4.7 Myungran-Jeots were about 12, 12, and 16 days, respectively.

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Preparation and Quality Characteristics of Enzymatic Salt-fermented Pearl Oyster, Pinctada fucata martensii (효소분해 진주조개(Pinctada fucata martensii) 젓갈의 제조 및 품질특성)

  • Kim, In-Soo;Kim, Hye-Suk;Han, Byoung-Wook;Kang, Kyung-Tae;Park, Jeong-Min;Oh, Hyeun-Seok;Han, Gang-Uk;Kim, Jin-Soo;Heu, Min-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.39 no.1
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    • pp.9-15
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    • 2006
  • As a part of the investigation for utilizing pearl oyster by-products, a rapid salt-fermented pearl oyster using commercial enzyme was prepared and also examined on the characteristics. The salt-fermented pearl oyster prepared by optimal condition, which was prepared by mixing of minced pearl oyster, 15% salt, and 1% $Protamex^\circledR$ and fermented for 4 weeks, was superior in hydrolysis degree (28.7%) and ACE inhibitory activity (92.6%) to salt-fermented pearl oyster prepared by other conditions, such as the use of whole tissue, different enzymes $(Alcalase^\circledR,\;Neutrase^\circledR\;and\;Flavourzyme^\circledR)$, different salt concentrations (20 and 25%), and different fermentation periods (2, 6 and 8 weeks). There were, however, some shortcomings with this product. It showed a dark green color and an unfavorable bitter taste. These shortcomings were improved by the addition of seasoning paste. The calcium and phosphorus contents of the seasoned salt-fermented pearl oyster were 64.2 mg/100 g and 71.6 mg/100 g, respectively, and the calcium content based on phosphorus was a good ratio for absorbing calcium. The total amino acid content of the seasoned and salt-fermented pearl oyster was 7,054 mg/100 g and the major amino acids ware aspartic acid (555.1 mg/100 g), glutamic acid (1,131.2 mg/100 g), alanine (658.2 mg/100 g), and lysine (695.5 mg/100 g). The seasoned salt-fermented pearl oyster, along with angiotensin I converting enzyme (ACE) inhibitory activity (98.3%), also showed a recognizable level (87.5%) of anti-oxidative activity.

Degradation of Nucleotides and Their Related Compounds During the Fermentation of Oyster (굴젓 숙성중(熟成中) 핵산(核酸) 관련물질(關聯物質)의 변화(變化))

  • Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.7 no.2
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    • pp.1-6
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    • 1978
  • Changes of nucleotides and their related compounds during the fermentation of oyster were analyzed by high speed liquid chromatography. In raw oyster, dominant 5'-UMP was $26.1{\mu}mole/g$ and the content of uracil, hypoxanthine, 5'-GMP were 5.2, 3.8, 2.8 and $2.7{\mu}mole/g$ on moisture and salt free base, respectively. The content of cytosine, 2',3'-CMP, 5'-AMP and 2',3'-GMP were lower than $1.0{\mu}mole/g$ and guanine were detected in trace amount. 5'-UMP, uracil, hypoxanthine, 5'-IMP and 5'-GMP were abundant in both raw sample and fermented products. 5'-UMP and 5'-IMP were decreased slowly while 5'-AMP, 2'3'-CMP, cytosine and guanine were increased during the fermentation, and the increase of 5'-GMP and uracil were fluctuated. The content of hypoxanthine in raw oyster was increased to 3.1, 4.2 and 7.7 times of raw sample after 19, 36 and 68 days of fermentation, respectively.

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Effect of Garlic on Qua lily of Low Salted Anchovy - 1. Changes of general composition, titrable acidity and sensory evaluation - (마늘의 첨가가 저염 멸치젓의 품질에 미치는 영향 - 제1보 관능적 변화 및 일반성분, 산도 및 관능검사 -)

  • 진양호;권오천;성낙주;신정혜;강민정
    • Culinary science and hospitality research
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    • v.7 no.2
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    • pp.49-70
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    • 2001
  • The anchovy, Engraulis japonica, were prepared with two different saltconcentration of 20%, 10% which was added 2, 5, 8 and 10 % of grind garlic(LSA 1, 2, 3, 4) and garlic juice(LSB 1, 2, 3, 4), respectively. The changes of such factor during fermentation of anchovy as general composition, salt concentration, titrable acidity and sensory evolution were analyzed. In addition, chewiness, taste, garlic flavor and off-flavor wastested it's results were belived that the more garlic amounts added the more rotten smell has been disappeared. In the view of sensory perception. sample of 90 days fermentation is supposed as the best period of fermentation. The contents of moisture and crude fat decreased while, those of ash and crude protein were changes little during fermentation of low salted anchovy. Titrable acidity decreased in all experiment groups during anchovy fermentation, its contents at 30 days fermentation were ranged between 1.4~ 1.8g/100g, but it dramatically decreased to 0.6~l.1g/100g at 90 days fermentation.

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Changes of S-Adenosyl-L-Methionine (SAM) in Kimchi Using Different Raw Materials (기능성 김치 제조를 위한 김치 원 부재료에 따른 S-adenosyl-L-methionine(SAM) 함량의 변화)

  • Lee, Myung-Ki;Lee, Hyun-Jung;Park, Wan-Soo;Koo, Kyung-Hyung;Kim, Young-Jin;Jang, Dai-Ja;Suh, Joo-Won
    • Korean Journal of Food Science and Technology
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    • v.41 no.4
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    • pp.417-422
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    • 2009
  • The purpose of this study was to measure the changes in S-adenosyl-L-methionine (SAM) content and to find the best condition for SAM Kimchi during fermentation with the different kinds of raw materials of Kimchi and the diverse ways of making Kimchi. As fermentation was processing, pH of all Kimchi groups dramatically decreases at the beginning stage of experimentation. However, pH value was 4.2-4.3 in the last stage. Titratable acidity tends toward the similar results in pH value. At the first, the SAM content went down time substantially and then increases. Kimchi (A), which was made of the most basic raw materials, resulted in the lowest content of SAM. The most abundant SAM content of Kimchi was the Kimchi made with certain materials. Kimchi (I) had the most has SAM content, overall. The best time of fermentation was when pH was between 4.3 and 5.3, and titratable acidity was 0.5-1.0%. As the results of this study, the highest SAM content in Kimchi could be made when Kimchi was fermented for 9-12 days and titratable acidity showed 0.5-1.0% This study proved that the ratio of raw materials such as red pepper, fermented fished sauces, and other materials improved the levels of SAM in the Kimchi.

Quality and sensory characteristics of commercial kimchi according to sodium contents (나트륨 함량에 따른 시판 배추김치의 품질과 관능적 특성)

  • Hwang, Eun-Sun;Kim, Hyo Sung;Kim, Soo Hyun;Ko, Hyun Joo;Lee, Mi Young;Yoon, Eun-Kyung
    • Korean Journal of Food Science and Technology
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    • v.48 no.5
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    • pp.413-417
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    • 2016
  • This study was conducted to compare the quality and sensory characteristics of commercial kimchi containing different sodium contents. The salinity at day 1 post-manufacture in regular kimchi was 1.99%, while it was 1.56% in lowsodium kimchi, thus showing a 21.6% reduction in sodium content. The pH of low-sodium kimchi was much lower than that of regular kimchi and the pH was dramatically decreased in both samples after 5 days of storage. The total acidity of low-sodium kimchi was higher than that of the regular kimchi, and increased during the storage period. The number of lactic acid bacteria was maximum at day 5 but slightly decreased after 10 days of storage. The sensory evaluation panels preferred low-sodium kimchi and realized that the saltiness of low-sodium kimchi was less than that of the regular kind. Based on these results, the quality characteristics of low-sodium kimchi were competitive to those of regular kimchi.

Quality Changes during Storage of Low Salt Fermented Anchovy treated with High Hydrostatic Pressure (초고압 처리한 멸치젓의 저장 중 품질 변화)

  • Lim, Sang-Bin;Jwa, Mi-Kyung;Mok, Chul-Kyoon;Woo, Gun-Jo
    • Korean Journal of Food Science and Technology
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    • v.32 no.2
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    • pp.373-379
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    • 2000
  • Low salt fermented anchovy was stored at $25^{\circ}C$ for a period of 20 days from the time of ultra-high pressure treatment under different operating conditions, such as magnitude of pressure($(200{\sim}500\;MPa)$, temperature$(20{\sim}50^{\circ}C)$ and treatment time$(5{\sim}20\;min)$ with viable cell count(VCC) and quality assessments conducted at regular intervals. VCC decreased logarithmically during storage. Lower values of VCC in the treated samples were observed compared to the untreated. A gradual increase in peroxide value was noticed during storage, compared to those of the untreated which showed a sudden rise. Thiobarbituric acid value decreased initially and remained at that level before rising almost exponentially between 12 and 20 days. Volatile basic nitrogen increased gradually during storage. Amino nitrogen remained almost constant up to 20 days, regardless of any conditions investigated. High pressure treatment maintained better quality during storage at $25^{\circ}C$ by reducing the viable cell count in low salt fermented anchovy.

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