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Insect Resistance of Tobacco Plant Expressing CpBV-ELP1 Derived from a Polydnavirus (폴리드나바이러스 유래 CpBV-ELP1 발현 담배의 내충성)

  • Kim, Eunseong;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.56 no.1
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    • pp.19-28
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    • 2017
  • Polydnaviruses (PDVs) are a group of double-stranded DNA viruses symbiotic to some endoparasitoid wasps. Cotesia plutellae bracovirus (CpBV) is a PDV symbiotic to an endoparasitoid wasp, C. plutellae, parasitizing young larvae of Plutella xylostella. An early expressed gene, CpBV-ELP1, plays an important role in the parasitism by suppressing host cellular immunity by its cytotoxic activity against hemocytes. This study aimed to test its oral toxicity against insect pest by expressing it in a recombinant tobacco plant. A recombinant CpBV-ELP1 protein was produced using a baculovirus expression system and secreted to cell culture medium. The cell cultured media were used to purify CpBV-ELP1 by a sequential array of purification steps: ammonium sulfate fractionation, size exclusion chromatography, and ion exchange chromatography. Purified rCpBV-ELP1 exhibited a significant cytotoxicity against Spodoptera exigua hemocytes. CpBV-ELP1 was highly toxic to the fifth instar larvae of S. exigua by injection to hemocoel. It also showed a significant oral toxicity to fifth instar larvae of S. exigua by a leaf-dipping assay. CpBV-ELP1 was cloned into pBI121 vector under CaMV 35S promoter with opaline synthase terminator. Resulting recombinant vector (pBI121-ELP1) was used to transform Agrobacterium tumefaciens LBA4404. The recombinant bacteria were then used to induce callus of a tobacco (Nicotiana tabacum Xanthi) leaves and subsequent generation (T1) plants were selected. T1 generation tobacco plants expressing CpBV-ELP1 gave significant insecticidal activities against S. exigua larvae. These results suggest that CpBV-ELP1 gene can be used to control insect pests by constructing transgenic crops.

Innovation and Future Skills Needs-Green Car (기술혁신과 미래숙련수요 대응-그린카 발전을 중심으로)

  • Hwang, Gyu-Hee;Lee, Joong-Man
    • Journal of Korea Technology Innovation Society
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    • v.13 no.3
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    • pp.399-422
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    • 2010
  • Global expansion of regulation for carbon emission has brought new industrial regulations and trade barriers while the global economy is promoting economic stimulus through a variety of green investments such as renewable energy development, energy efficiency increase, and environmental improvement simultaneously. Korean government is trying to seek a new opportunity to grow through green investment in this change of business environment and businesses are facing the transition to low-carbon and eco-friendly structure for their survival. It is promoting not only industry structure changes and conversion but also demanding changes of workforce composition and requirement skills. In response to convergence of green economy, manpower development for future skills needs is provided the main impetus for response as well as promotion green growth. This study will analyze human resource development plan due to future skills needs emphasizing on automative industry. Also the study will be researched on the problems of present curriculum of undergraduate and look for improvements tasks. Upon the result, promotion plans for human resource development in other sectors of green car in response to future skills needs in green growth can be found.

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Toxicity Reduction of VOCs by Catalytic Oxidation Mechanism (VOCs의 촉매산화 메커니즘에 의한 독성 저감효과)

  • 이재동
    • Journal of environmental and Sanitary engineering
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    • v.15 no.3
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    • pp.88-93
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    • 2000
  • The objective of this study was to depict the kinetic behavior of the platinum catalyst for the deep oxidation of VOCs and their mixture. The oxidation characteristics of VOCs, which were benzene, toluene, and styrene, was studies on a 0.5% $Pt/{\gamma}-Al_2O_3$ catalyst. The reactivity increases in order benzene>toluene>styrene. In mixtures, remarkable effects on reaction rate and selectivity have been evident ; the strongest inhibiting effect was shown by styrene and increases in a reverse order with respect to that of reactivity. The reaction model reveals that there is a competition between the two reactants for the oxidized catalyst. Thus, the nontoxic catalytic oxidation process was suggested as the new VOCs control technology.

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The Analysis on the Effect on the Competition Types of Switching Cost in Telecommunications (정보통신산업의 전환비용과 기업간 경쟁양상)

  • Park, Wung;Jung, Young-Sic;Min, Jae-Hong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2002.05a
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    • pp.400-402
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    • 2002
  • Recently, it is requested interoperability and inter-connection among technologies due to network externalities and the rapid innovation in the telecommunications industry. Firms are technically tied up with the others the survival in the keen competition age. The switching cost is caused when a consumer changes his existing service to new one in the telecommunications. The switching cost hinders consumers from making a choice of better services or goods, and obstruct the fair competition among firms. In this paper, we will examin the effects on the competition among companies of the switching cost, and suggest national policies to promote the competition in the telecommunication markets.

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The Effects of the Feuerstein's Cognitive Mediated Learning for Gifted Children on Attention Control and Attention Shift (Feuerstein의 인지적 중재학습이 영재아의 주의통제와 주의전환에 미치는 효과)

  • Yang, Yeon-Suk;Kil, Kyung-Suk
    • Journal of Gifted/Talented Education
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    • v.20 no.3
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    • pp.967-984
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    • 2010
  • This study purposed to examine the effects of the Feuerstein's cognitive mediated learning for gifted children on attention control and attention shift. For the study, 40 gifted students were chosen from the 3rd graders in elementary schools and divided into test and control groups using K-WISC-III and Torrance Tests of Creative Thinking. The mediated learning program that is targeted to improve the executive functions of gifted children has used the tools of Organization of Dots, Analytic Perception and Comparisons among Feuerstein's Instrumental Enrichment(FIE). According to the results of this study, a significant improvement has been observed in selective attention, self-control, sustained attention, and attention shift through cognitive mediated learning. Therefore, it has been proven that the cognitive mediated learning is effective in reducing gifted children's problematic behaviors that are caused by a lack of attention control and attention shift and improving their cognitive functions and potentials.

Structural Transformation of Exports in A Product Space Model: The Case of Daegu-Gyeongbuk Province, Korea (생산물공간 분석에 의한 대구경북 수출산업의 구조전환에 대한 연구)

  • Lee, Byeong-Wan;Park, Jin-Ho
    • Korea Trade Review
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    • v.42 no.1
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    • pp.47-67
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    • 2017
  • This paper analyzes industries of the Daegu-Gyeongbuk province of the Republic of Korea using the product space model that was developed mid 2000s on the basis of a network analysis framework. The study examines the structure of the product space for the 421 export items at HS 4-digit level after grouping them into 4 categories; classics, emerging champions, marginals and the disappearing products. The results suggest a significant structural transformation in the product space for the local industries and the scope and magnitude of such transformation was rather large. We were also able to confirm that the structural transformation of the product space differs from industry to industry and from group to group. While the approach used is not without shortcomings, our findings also indicate that information extracted from the world merchandise trade by way of product space indicators can be quite useful in identifying structural transformation of industries.

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Fast Scene Change Detection Using Macro Block Information and Spatio-temporal Histogram (매크로 블록 정보와 시공간 히스토그램을 이용한 빠른 장면전환검출)

  • Jin, Ju-Kyong;Cho, Ju-Hee;Jeong, Jae-Hyup;Jeong, Dong-Suk
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.48 no.1
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    • pp.141-148
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    • 2011
  • Most of the previous works on scene change detection algorithm focus on the detection of abrupt rather than gradual changes. In general, gradual scene change detection algorithms require heavy computation. Some of those approaches don't consider the error factors such as flashlights, camera or object movements, and special effects. Many scenes change detection algorithms based on the histogram show better performances than other approaches, but they have computation load problem. In this paper, we proposed a scene change detection algorithm with fast and accurate performance using the vertical and horizontal blocked slice images and their macro block informations. We apply graph cut partitioning algorithm for clustering and partitioning of video sequence using generated spatio-temporal histogram. When making spatio-temporal histogram, we only use the central block on vertical and horizontal direction for performance improvement. To detect camera and object movement as well as various special effects accurately, we utilize the motion vector and type information of the macro block.

The Effect on the Switching Intention to the Blockchain-based Supply Chain Management Information System (블록체인 기반 공급망관리 정보시스템으로의 전환의도에 영향을 미치는 요인)

  • Kyoung Sang Oh;Dong Myung Lee
    • Journal of Industrial Convergence
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    • v.20 no.12
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    • pp.11-25
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    • 2022
  • In this study, we want to verify the factors that affect the intention to switch to a supply chain management information system applied with blockchain. To this end, variable selection and research model were constructed through the review of previous studies, and empirical analysis was conducted using the TOE framework and PPM model. The effects of Push and Pull factors on the intention to switch to the block chain system and the moderating effect through the switching cost which is a Mooring factor, were verified. The hypothesis was verified using a structural equation model using a sample of 320 response data by conducting a questionnaire survey on small and medium-sized enterprises located in Korea. As a result of the study, social influence, which is a push factor, and management's will to innovate, which is a Pull factor, had a significant effect on switching intention. And the moderating effect between the groups with high and low switching cost recognition was confirmed. This study is significant in that it presents the concept and research direction of SCBM (supply chain & blockchain management) that can enhance the competitiveness of a company through the implementation of a blockchain-based supply chain management information system.

회원사 탐방-캐논코리아비즈니스솔루션

  • 박지연
    • The Optical Journal
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    • s.118
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    • pp.40-42
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    • 2008
  • 하드웨어사업에서 통합문서관리서비스 사업으로 전환한 캐논코리아비즈니스솔루션(대표 . 김천주, www.canon-bs.co.kr)은 문서보안, 통합관리, 전자문서관리 등 기업의 문서를 종합적으로 아우를 수 있는 솔루션과 전문 인력의 '컨설턴트'의 경험을 바탕으로 확실한 경쟁우위를 보이겠다는 각오이다. 캐논코리아측은 이 같은 의지를 반영하듯 지난 10월 본사 비즈니스 플라자 내에 캐논코리아의 통합문서관리시스템의 기술력을 집약해 놓은 '솔루션룸'을 새롭게 단장하고 첨단 기업 비즈니스 환경의 새로운 대안을 제시하고 있다. 캐논코리아비즈니스솔루션 솔루션지원팀의 박정우 과장의 안내를 받아 솔루션룸의 면면을 살펴보았다.

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방위산업의 구조조정 방안 고찰

  • O, Won-Seok
    • Defense and Technology
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    • no.12 s.286
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    • pp.66-73
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    • 2002
  • 점점 첨단/복합화.고가화 되어 가고 있는 무기체계 요구에 부응할 수 있또록 국내 방위산업의 공급능력/수준을 향상시키기 위해서는 방위산업 및 기술기반을 군사력의 일부로 인식하는 사고의 전환이 시급하며, 국방연구개발 투자의 확대 및 효율적 집행과 더불어 세계시장 지향적인 방위산업으로의 구조조정이 요망된다. 미국과 유럽국가들의 거대 방산업체들이 세계 방산시장을 과점적으로 지배하고 있는 방산현실에서 이들 기업들과 경쟁할 수 있는 분야와 틈새시장을 발견하여 우리의 독자적인 방산능력을 제고시키는 노력과 더불어 방산업체 차원에서의 국제협력을 통한 생존전략의 모색이 시급하다.

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