• Title/Summary/Keyword: 전체 유전체

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Complete genome sequence of biofilm-producing strain Staphylococcus xylosus S170 (생물막 생성 Staphylococcus xylosus S170 균주의 유전체 분석연구)

  • Hong, Jisoo;Roh, Eunjung
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.167-168
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    • 2018
  • Here we report the complete genome sequence of Staphylococcus xylosus S170, strong biofilm-producing strain, which comprised a single circular 2,910,005 bp chromosome and 32.97% G + C content. The genome included 2,674 protein-coding sequences, 22 rRNA genes, and 57 tRNA genes. Gene analysis of S. xylosus S170 could contribute to better understanding of biofilm-forming mechanisms.

The 3rd Generation Genome Map of the Korean Cattle (Hanwoo) (제3세대 한우유전체지도작성)

  • Lee, Yong-Seok;Choi, In-Ho
    • Journal of Animal Science and Technology
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    • v.51 no.2
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    • pp.123-128
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    • 2009
  • Recently, the $2^{nd}$ generation genome map of the Korean cattle (Hanwoo) has been constructed by comparison of the nucleotide sequence of the Korean cattle BAC clones with whole genome sequence of the bovine data-base (B_tau 2.1 build). The objective of this study was to update the $2^{nd}$ generation genome map of the Korean cattle using the similar approach. The nucleotide sequence of the Korean cattle BAC clones utilized in the construction of the $2^{nd}$ generation map was compared with the newly released bovine data-base (B_tau 3.1 build) to generate the $3^{rd}$ generation map. While, 5,105 BAC clones were localized on bovine chromosome in the $2^{nd}$ generation map, a total of 9,595 BAC clones, which spans about 37.27% of the bovine chromosome after eliminating the overlapping sequence among the clones, have been mapped on the bovine chromosome in the $3^{rd}$ generation map. Further analysis of the nucleotide sequence of the BAC clones will allow us to develop map and facilitate to pinpoint the genes that are important for the improvement of the performance in this cattle breed.

Human Genome Project (인간유전체 사업)

  • Kwon, Oh-Joo
    • Korean Journal of Biological Psychiatry
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    • v.8 no.2
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    • pp.196-202
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    • 2001
  • The completion of the rough draft of the human genome is a remarkable achievement. It provides the overall structures of huge DNA molecules that constitute the genome and an outline of the information needed to create a human being. This paper reviewed new ideas, projects, and scientific advances made by the Human Genome Project. We also discussed the future of medicine and biomedical research in postgenomic era.

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Analysis of Probabilistic Limits of Trait Identity in Inter-Strain Comparison of Genomic Fingerprints of Bacteria (균주간 유전체 지문 비교분석에서 유전형질 일치성의 확률적 한계 분석)

  • Zo, Young-Gun
    • Korean Journal of Microbiology
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    • v.47 no.3
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    • pp.263-267
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    • 2011
  • Genomic fingerprinting methods are useful in determining relatedness among bacterial strains. However, random coincidences in sizes of two DNA fragments in two different fingerprints may occur, resulting in erroneous interpretation of relatedness between two bacterial genomes. In this study, I estimated the probability of occurrence of DNA bands of identical size in fingerprints of two unrelated genomes, so that the significance of fingerprint-based estimation of genome relatedness could be analyzed. The probability could be estimated as outputs of a function formulated with the three parameters: the numbers of observed fragments, all possible sizes of fragments and observed fragments common in a given pair of fingerprints. The parameter most instrumental to significance of relatedness estimation was the number of all possible sizes of fragments. To keep the number of coincidentally-common size of fragments below 10, about 200 fragments should be distinguishable in the fingerprints.

The Algorithm of implementation for genome analysis ecosystems : Mitochondria's case (유전체 생태계 분석을 위한 알고리즘 구현: 미토콘드리아 사례)

  • Choi, Sung-Ja;Cho, Han-Wook
    • Journal of Digital Convergence
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    • v.14 no.4
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    • pp.349-353
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    • 2016
  • The studies on the human environment and ecosystem analysis is being actively researched. In recent years, The service of genome analysis has been offering the customized service to prevent the disease as reading an individual's genome information. The genome information by analyzing technology is being required accurate and fast analyses of ecosystem-dielectrics due to the spread of the disease, the use of genetically modified organism and the influx of exotic. In this paper the algorithm of K-Mean clustering for a new classification system was utilized. It will provide new dielectrics information as quickly and accurately for many biologists.

Design and Implementation of the genome-level fragment assembly system, Mater (Fragment Assembly를 위한 시스템의 설계 및 구현)

  • 김명선;정철희;박현석
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.04a
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    • pp.751-753
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    • 2001
  • 지금까지 인간이나 다른 생물체의 전체 유전체 염기서열을 밝혀내는 작업은 크게 세가지 방법으로 진행되었다. Clone-by-clone approach, sequence tagged connector approach, random shotgun approach[1]가 그것인데 마지막의 random shotgun approach는 fragment assembly problem을 비롯한 여러 가지 전산학적인 문제들을 수반한다. 미생물체의 전체 염기서열을 random shotgun approach를 이용하여 밝혀낼 때 몇 가지 전산학적인 문제가 테크닉이 필요하며 그 중에서도 서열간의 forward, reverse의 mating 정보를 이용하는 것이 중요하다. 본 논문은 이러한 mating 작업을 한 눈에 볼 수 있게 하는 소프트웨어 페키지 “Mater”에 대해 소개하고자 하며 그 의미에 대해 논하고자 한다.

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Development of PCR-based markers for selecting plastid genotypes of Solanum hjertingii (Solanum hjertingii 색소체 유전자형 선발을 위한 PCR 기반 분자마커 개발)

  • Tae-Ho Park
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.34-44
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    • 2023
  • The tetraploid Solanum hjertingii, a wild tuber-bearing species from Mexico is a relative of potato, S. tuberosum. The species has been identified as a potential source of resistance to blackening for potato breeding. It does not exhibit enzymatic browning nor blackspot which are physiological disorders. However, due to their sexual incompatibility, somatic hybridization between S. hjertingii and S. tuberosum must be used to introduce various traits from this wild species into potato. After somatic hybridization, molecular markers are essential for selecting fusion products. In this study, the chloroplast genome of S. hjertingii was sequenced by next-generation sequencing technology and compared with those of other Solanum species to develop specific markers for S. hjertingii. The chloroplast genome has a total sequence length of 155,545 bp, and its size, gene content, order and orientation are similar to those of the other Solanum species. Phylogenic analysis including 15 other Solanaceae species grouped S. hjertingii with S. demissum, S. hougasii, and S. stoloniferum. After detailed comparisons of the chloroplast genome sequence with eight other Solanum species, we identified one InDel and seven SNPs specific to S. hjertingii. Based on these, five PCR-based markers were developed for discriminating S. hjertingii from other Solanum species. The results obtained in this study will aid in exploring the evolutionary aspects of Solanum species and accelerating breeding using S. hjertingii.

Development of microarrayer for manufacturing DNA chip used in genome project (II) - The performance test of developed robot system (유전자 검색을 위한 DNA chip 제작용 로봇 시스템의 개발(II) - 로봇 시스템의 성능실험)

  • 이현동;김기대;김찬수;김성환;나건영;임용표
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2002.07a
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    • pp.333-338
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    • 2002
  • 인간 게놈 프로젝트가 지속적으로 진행됨에 따라 계속적으로 대량의 유전체 정보가 밝혀지고 있으며, 이미 밝혀진 유전체의 염기서열을 바탕으로 다양한 생물의 전체 유전자의 기능을 효율적으로 해석하는 기술의 개발이 요구되고 있다. 식물 게놈 프로젝트 또한 식량확보라는 단순하면서도 전략적인 차원에서 가장 절실히 요구되는 기본 과학기술 연구분야이다. (중략)

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