• Korean Journal of Plant Tissue Culture
• /
• v.22 no.6
• /
• pp.329-334
• /
• 1995

Transformation system of rolC gene, dwarf gene in Lycium chinenese Mill. established by using system. Pin-punctured leaves induced numerous adventious buds in abaxial side when cultured on 3/2 MS medium containing 2.0 mg/L zeatin. Survival rate and shoot regeneration frequency of leaf explants decreased as kanamycin sulfate level increased. Shoot buds were not regenerated on 3/2 MS medium containing 10 mg/L kanamycin sulfate and 2.0 mg/L zeaein. Of the level tested, 10 mg/L of kanamycin sulfate was optimum in selection of kanamycin sulfate resistant plant. Co-culture time of bacteria and leaf explants was affected at the frequency of shoot regeneration and survival of leaf explants. Leaf explants co-cultivated during above 48hr severely decreased survival rate and shooting rate. Best result on survival rate and shooting rate were obtained when exposed for 24 h. 80 explants of 105 leaf explants survived on 3/2 MS medium containing 2.0 mg/L zeatin and 10 mg/L kanamycin sulfate, and 15 shoots was regenerated on the same medium. To select kanamycin sulfate resistant plant, regenerate as cultured on 3/2 MS medium containing 10 mg/L kanamycin sulfate, and obtained 5 kanamycin resistant plants. Southern blot analysis conformed that the rolC gene was incorporated into the genomic DNA of kanamycin resistant plants.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.15 no.6
• /
• pp.477-484
• /
• 2022

In this study, the cartridge part of the focused ultrasound stimulation system was used as a sample to conduct a skin irritation test and a skin sensitization test through animal experiments among the tests related to GLP (Good Laboratory Practice), a medical device safety evaluation standard. The test was conducted after IACUC approval using 6 female New Zeland White Rabbits. The polar and non-polar stimulation indices were all '0.0'. In addition, in the case of skin sensitization evaluation, 30 guinea pigs approved by IACUC were divided into control and experimental groups, and all induction and induction steps were applied using the eluate itself without separate dilution of the test sample and blank test solution. As a result of the experiment, the skin reaction grade of the control animal was 0 grade, and there was no animal showing more than 1 grade in the skin reaction of the test animal. As a result of both tests, no abnormal skin symptoms were observed, and when applied to the human body to treat patients, the test materials used in the tests will investigate the stability of whether any diseases that cause skin abnormal symptoms will occur.

• The Journal of the Korea Contents Association
• /
• v.15 no.8
• /
• pp.473-480
• /
• 2015

The purpose of this study is to analyze the influence of the cash flow of pharmaceutical companies on R&D investment. 143 pharmaceutical companies listed in the KOSDAQ market from 2009 to 2013. Financial statements and comments in general and internal transactions were extracted from TS-2000 of the Korea Listed Company Association (KLCA), and data related to stock price was extracted from KISVALUE-III of NICE Information Service Co., Ltd. STATA 12.0 was used as the statistical package for panel analysis. The summary of the findings and the interpretation of the significance of this are as follows: First, the current ratio (internal finance) had a positive influence on R&D investment. Second, the debt ratio (external finance) had a negative influence on R&D investment. The pharmaceutical company prefers internal funds to external funds due to the asymmetry of information in the loan markets. In other words, this shows why internal finances have a significant influence on R&D investment at pharmaceutical companies.

• The Journal of the Korea Contents Association
• /
• v.15 no.6
• /
• pp.445-454
• /
• 2015

The purpose of this study is to analyze the influence of the financial structure of pharmaceutical companies on R&D investment. 358 pharmaceutical firms listed in the KOSDAQ market from 2000 to 2012. Financial statements and comments in general and internal transactions were extracted from TS-2000 of the Korea Listed Company Association (KLCA), and data related to stock price was extracted from KISVALUE-III of NICE Information Service Co., Ltd. STATA 12.0 was used as the statistical package for panel analysis. The summary of the findings and the interpretation of the significance of this are as follows: First, the shareholding ratio of major shareholders and foreigners had a positive influence on R&D investment. Second, the ratio of outside directors had a negative influence on R&D investment. Third, the shareholding ratio of institutional investors did not have a significant influence on R&D investment.

• Journal of Biosystems Engineering
• /
• v.7 no.2
• /
• pp.57-71
• /
• 1983

마찰식 정미기의 성능에 영향을 미치는 기계설계 및 작동상의 인자로는 스크린의 형태, 롤러의 형태, 롤러회전속도, 출구 저항 등 여러가지가 있다. 이러한 요인들에 대한 연구가 본 논문의 저자에 의해서 시리즈로 연구되어 왔으며, 본 연구는 그의 일환으로 스크린의 단면형상(6각, 8각, 12각 그리고 원통형), 스크린에 뚫려있는 슬랏의 기울기($15^{\circ}$, $30^{\circ}$, $45^{\circ}$ 그리고 $-45^{\circ}$), 그리고 출구 저항이 정백미의 질과 양, 정백효율 그리고 정백실내의 정백압력 등에 미치는 영향을 규명하기 위해 수행되었다. 이 실험에 사용된 공시 정미기는 임도정 공장에서 널리 사용되고 있는 흡입마찰식이었며, 사용된 시료는 밀양 23호인 통일계품종이었다. 각 처리마다 동력 소모량과 정백실내의 정백압력을 측정하기 위해서 "KYOWA" 스트레인 게이지 시스템(strain gauge system), 토오크 변환기, 압력변환기 등이 사용되었다. 본 실험 결과를 요약하면 다음과 같다. 1) 정백과정중에 정백실내의 정백압력은 입구에서 가장 높았고 출구쪽으로 갈수록 점차 감소하였다. 평균 정백압력도 순환 횟수에 따라서 변화 하였는데 첫번째 순환에서 가장 높았고 순환횟수가 증가함에 따라 감소하였다. 2) 본 실험에서 사용한 출구저항 수준은 출구저항 수준 1에서 요구되는 정백도의 백미를 얻기 위한 순환 횟수는 2회 이었고 출구저항 수준 2에서는 4회 이었는데, 이를 출구저항 수준이 정백압력, 정백율, 완전미수율 및 도정 효율에 가장 큰 영향을 주었다. 3) 스크린에 뚫려있는 슬랏 각도가 $15^{\circ}$에서 $45^{\circ}$로 증가함에 따라서 정백실내의 평균 정백압력은 감소하였고, 완전미수율과 도정 효율은 증가하였으나, 일반적으로 도정수율은 감소하는 경향을 보였다. $-45^{\circ}$의 슬랏 각도를 가진 스크린에서는 슬랏 각도 $15^{\circ}$인 스크린에서와 거의 비슷한 정백압력 수준을 나타냈으나 완전미와 정백미의 생산은 매우 낮았다. 4) 스크린 표면에 기다란 강편 (보통 "띠"라고 부름)을 붙인 원통과 12각형의 스크린에서는 강편을 붙이지 않은 6각형과 8각형 스크린에서 보다는 비교적 높은 정백압력을 나타냈다. 전자는 후자에 비해서 정백효율은 높았으나 완전미수율은 떨어졌다. 5) 정백실내에서 가장 낮은 정백압력을 나타낸 8각형 스크린은 높은 도정수율과 완전미수율을 가져왔으나 상대적으로 정백효율은 낮게 나타났다. 6) 정백실내의 반경 방향의 평균 정백압력($P_R$)과 완전미수율($Y_h$)은 다음과 같은 1차적인 역비례 관계가 있었으며(평균 정백압력 범위는 0.5-0.9kg/$cm^2$), $Y_h=-28.661P_R+84.860$ ($r^2=0.858$) 정백효율($Y_e$)과 정백압력($P_R$) 사이에는 다음과 같은 2차적인 관계가 존재함을 알 수 있었다. $Y_e=-597.5P_{R^2}+929.96P_R-210.15$ ($r^2=0.759$) 정백효율은 정백압력이 0.7-0.8kg/$cm^2$일 때 가장 높았으며, 이때 변이도 가장 심한 것으로 나타났다.

• Journal of Life Science
• /
• v.17 no.12
• /
• pp.1660-1668
• /
• 2007

The non-ionic surfactant (NIS) Tween 80 was evaluated for its ability to influence invitro cumulative gas production, dry matter digestibility, cellulolytic enzyme activities, anaerobic microbial growth rates, and adhesion to substrates by mixed rumen microorganisms on rice straw, alfalfa hay, cellulose filter paper and tall fescue hay. The addition of NIS Tween 80 at a level of 0.05% increased significantly (P<0.05) in vitro DM digestibility, cumulative gas production, microbial growth rate and cellulolytic enzyme activity from all of substrates used in this study. In vitro cumulative gas production from the NIS-treated substrates; rice straw, alfalfa hay, filter paper and tall fescue hay was significantly (P<0.05) improved by 274.8, 235.2, 231.1 and 719.5% compared with the control, when substrates were incubated for 48 hr in vitro. The addition of 0.05% NIS Tween 80 to cultures growing on alfalfa hay resulted in a significant increase in CMCase (38.1%), xylanase (121.4%), Avicelase (not changed) and amylase (38.2%) activities after 36 h incubation. These results indicated that the addition of 0.05% Tween 80 could greatly stimulate the release of some kinds of cellulolytic enzymes without decreasing cell growth rate in contrast to trends reported with aerobic microorganism. Our SEM observation showed that NIS Tween. 80 did not influence the microbial adhesion to substrates used in the study. Present data clearly show that improved gas production, DM digestibility and cellulolytic enzyme activity by Tween 80 is not due to increased bacterial adhesion on the substrates.

• Journal of Korea Port Economic Association
• /
• v.40 no.2
• /
• pp.65-78
• /
• 2024

This study investigates the correlation between financial constraints and investment activities in Korean logistics firms. A sample of 340 companies engaged in the transportation sector, as per the 2021 KSIC, was selected for analysis. Financial data obtained from the DART were used to compile a panel dataset spanning from 1996 to 2021, totaling 6,155 observations. The research model was validated, and tests for heteroscedasticity and autocorrelation in the error terms were conducted considering the panel data structure. The relationship between investment activities in the previous period and current investment activities was analyzed using panel Generalized Method of Moments(GMM). The validation results of the research indicate that Korean logistics firms tend to increase investment activities as their level of financial constraints improves. Specifically, a positive relationship between the level of financial constraints and investment activities was consistently observed across all models. These findings suggest that investment decision-making varies based on the financial constraints faced by companies, aligning with previous research indicating that investment activities of constrained firms are subdued. Moreover, while the results from the model examining whether investment activities in the previous period affect current investment activities indicated an influence of investment activities from the previous period on current investment activities, the investment activities from two periods ago did not show a significant relationship with current investment activities. Among the control variables, firm size and cash flow variables exhibited positive relationships, while debt size and asset diversification variables showed negative relationships. Thus, larger firm size and smoother cash flows were associated with more proactive investment activities, while high debt levels and extensive asset diversification appeared to constrain investment activities in logistics companies. These results interpret that under financial constraints, internal funding sources such as cash flows exhibit positive relationships, whereas external capital sources such as debt demonstrate negative relationships, consistent with empirical findings from previous research.