• Title/Summary/Keyword: 전이유전자

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The CT values Comparisons for Antibiotic Resistant Bacteria and Resistant Genes by Chlorination (항생제 내성균 및 유전자제거를 위한 염소 CT 값 비교)

  • Oh, Jun-Sik;Kim, Sungpyo
    • Journal of Wetlands Research
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    • v.16 no.2
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    • pp.269-274
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    • 2014
  • The purpose of this study is to compare CT (disinfectant concentration * time) values in removing the antibiotic resistance bacteria, antibiotic resistance gene and transfer of antibiotic resistance genes. Different concentration of chlorine(C) and contact time(T) according to the removal of antibiotic resistance was calculated for each. As a result, for the 90% removal of antibiotic resistant bacteria, around 176~353 mg min/L CT values are needed. For the removal of the antibiotic resistance gene, 195~372 mg min/L CT values are required. For the 90% reduction of antibiotic resistance gene transfer by chlorine disinfection, 187~489 mg min/L CT values are needed. Based on our results, higher CT value was required for removing antibiotic resistant genes rather than antibiotic resistance bacteria.

Characterization of the RNA binding protein-1 gene promoter of the silkworm silk grands (누에 견사선에서 분리한 RNA binding protein-1 유전자 프로모터 분석)

  • Choi, Kwang-Ho;Kim, Seong-Ryul;Kim, Sung-Wan;Goo, Tae-Won;Kang, Seok-Woo;Park, Seoung-Won
    • Journal of Sericultural and Entomological Science
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    • v.52 no.1
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    • pp.39-44
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    • 2014
  • We isolated highly-expressed genes in the posterior silk glands of silkworm on a previously study, which one of these was identified as RNA binding protein-1 homologue (RBP-1) gene. In this study, we investigated gene expressional characteristics of the RBP-1 depending on silkworm development stages and several tissues of the larvae, respectively. Northern blot hybridization analysis showed that the RBP-1 gene was expressed high in larval and pupal periods, and highly expressed than endogenous internal control gene (BmA3) on all tested larval tissues. In addition, we isolated and analyzed a phage DNA having 1,660 bp-long promoter region of the RBP-1 gene from a genomic DNA library. To study the RBP-1 gene promoter activity, RBP-1 (-740/+ 30) was amplified by PCR and subcloned into a pGL3 basic vector to generate pGL-RBP1. A luciferase report vector carrying RBP-1 gene promoter (770 bp) was tested by luciferase assay in Sf9 cells. In the result, the RBP-1 gene promoter was more efficient than constitutive promoter (BmA3) by approximately ten percent.

Acquirement of transgenic rose plants from embryogenic calluses via Agrobacterium tumefaciens (배발생 캘러스를 이용한 아그로박테리움 매개형질전환 장미 식물체 획득)

  • Lee, Su-Young;Lee, Jung-Lim;Kim, Won-Hee;Kim, Seung-Tae;Lee, Eun-Kyung
    • Journal of Plant Biotechnology
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    • v.37 no.4
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    • pp.511-516
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    • 2010
  • The process to acquire intron-GUS gene-expressed transformants from somatic embryos (including embryogenic calli) of Rosa hybrida cv. 'Sweet Yellow' using Agrobacterium-meditated transformation method was reported in this study. Somatic embryos including embryogenic calluses were infected with Agrobacterium tumefaciens AGL1 strain (O.D = 0.7~1.6) including intron-GUS gene for 30 min, and were co-cultured for 3 days. After co-cultivation, they were cultured on embryo germination medium (EGM) supplemented with $250\;mg{\cdot}L^{-1}$ cefotaxim at $4^{\circ}C$ for 7 days. Then, transient GUS gene expression was observed. Shoots were regenerated from the shoot primodia induced from the intron-GUS gene-transferred either somatic embryos or embryogenic calli cultured on EGM supplemented with both cefotaxim $250\;mg{\cdot}L^{-1}$ and ppt $2\;mg{\cdot}L^{-1}$. Before induction of rooting from shoots cultured on shoot growing medium supplemented with both cefotaxim $250\;mg{\cdot}L^{-1}$ and ppt $2\;mg{\cdot}L^{-1}$, the shoots were cultured on multi-shoot induction medium supplemented with both cefotaxim $250\;mg{\cdot}L^{-1}$ and ppt $2\;mg{\cdot}L^{-1}$ to induce multi-shoots. When expression of the gene from a part of the multi-shoots was identified by GUS transient assay, the putative transgenic multishoots were transferred to rooting medium supplemented with cefotaxim $250\;mg{\cdot}L^{-1}$. After the formation of healthy roots, transgenic plantlets were transferred to the greenhouse after acclimatization. The expression rate of the intron-GUS gene in the multi-shoots was 100%.

DNA 메틸화 Code와 발생의 Epigenetics - DNA 메틸화 전이효소(Dnmt1) 발현의 Epigenetics 제어 -

  • 고응규
    • Proceedings of the KSAR Conference
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    • 2004.06a
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    • pp.176-182
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    • 2004
  • DNA 메틸화는 chromatin의 remodeling에 관여하고 유전자의 silencing 안정화 등, epigenetics 조절기구로서 중요하다. 포유류의 몸을 구성하는 다양한 조직과 세포는 각각 고유의 DNA 메틸화 패턴을 형성하고 있는 것이 최근의 연구에서 밝혀지고 있다. 개체발생 세포의 분화에 DNA 메틸화가 중요한 역할을 하고 있는 것이다. 본 강연에서는 DNA 메틸화 시스템에 대하여 개설하고, DNA 메틸화 전이효소(Dnmt1) 발현의 epigenetics 제어에 대하여 설명한다. Dnmtl은 제lexon의 구분에 따라 만들어지는 3종류의 iso-form이 존재한다. (중략)

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State Transition Analysis Using Multiple-Valued Logic Automata and Genetic Algorithm (다치오토마타와 유전자 알고리즘에 의한 상태 전이 해석)

  • 고현정;손창식;정환묵
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2004.04a
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    • pp.506-509
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    • 2004
  • 생물과 같이 외부 환경의 변화에 적응하는 능력을 갖도록 하기 위한 시스템을 다치오토마타를 사용하여 모델화하고 이들에 대하여 도태, 교배, 돌연변이 둥의 유전적 조작을 반복함 적용에 의해 유한 상태 전이 과정을 해석하고 응용할 수 있는 방법을 제안한다. 이러한 해석과 방법에 대한 모델을 기초로 자기 갱신할 수 있는 자율 오토마타와 환경에 적응할 수 있는 적응 오토마타를 실현하는 기초 단계로 적용할 수 있는 가능성을 제안한다.

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Regulation of GFP Expression Using the Tetracycline Inducible Retroviral Vector System (Tetracycline Inducible Retrovirus Vector System에 의한 GFP 유전자의 발현 조절)

  • Koo Bon Chul;Kwon Mo Sun;Kim Teoan
    • Reproductive and Developmental Biology
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    • v.29 no.1
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    • pp.57-62
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    • 2005
  • One of the critical problems to be solved in transgenic animal production is uncontrollable constitutive expression of foreign genes, which usually results in serious physiological disturbances in the transgenic animal. To circumvent this problem, we constructed and tested two retrovirus vectors designed to express the GFP(green fluorescent protein) gene under the control of the tetracycline-inducible promoters. To maximize the GFP gene expression at turn-on state, WPRE(woodchuck hepatitis virus posttranscriptional regulatory element) sequence was introduced into the retrovirus vectors at downstream region of either the GFP gene or the sequence encoding rtTA(reverse tetracycline-controlled transactivator). Transformed cells were cultured in the medium supplemented with or without doxycycline(tetracycline derivative) for 48 hours, and induction efficiency was measured by comparing the GFP gene expression level using fluorometry and western blotting. Higher GFP expression was observed from the vector carrying the WPRE sequence at 3' side of the GFP gene, while tighter expression control(up to 20 fold) was obtained from the vector in which the WPRE sequence was placed at 3' side of rtTA sequence. The resulting tetracycline inducible vector system may be used in transgenic animal production and gene therapy.

Expression of DNA Methyltransferase Transcripts in The Oocytes and Preimplantation Embryos in Mouse (생쥐 난자와 착상전 초기배아에서 DNA 메틸전이효소 전사물의 발현)

  • 김종월;이양한;강승호;한성원;전일경;김성례;김문규
    • Development and Reproduction
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    • v.2 no.2
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    • pp.197-203
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    • 1998
  • DNA methylation seems to play an important regulatory role in gene expression and cell differentiation during postimplantation embryonic development. However, the significance of DNA methylation which is maintained by the DNA MTase during preimplantation embryonic development, is not fully understood. In order to study the role of DNA methylation in the preimplantation embryos, the expression of DNA MTase transcripts was monitored in the oocytes and preimplantation embryos. The mRNA of DNA MTase was detected in the oocytes and pleimplantation embryos. The relative mRNA levels of DNA MTase were high from the stages of GV-oocytes and pronuclear embryos, and thereafter decreased gradually. By the treatment of $\alpha$-amanitin, it was confirmed that the transcripts presented in pronuclear embryos was derived from the maternal genome. The presence of transcripts of DNA MTase in the oocytes and pronuclear embryos suggests that the maintenance of DNA methylation may be necessary and seems to play an important role in gene expression and cell differentiation during preimplantation embryonic develop-ment in mouse.

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