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Characterization of the Stretch-Activated Channel in the Hamster Oocyte (햄스터난자에서 신전에 의해 활성화되는 통로의 성상)

  • Kim, Y.-M.;Hong, S.-G.
    • Journal of Embryo Transfer
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    • v.19 no.2
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    • pp.89-99
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    • 2004
  • Stretch-activated channels (SACs) responds to membrane stress with changes in open probability (Po). They play essential roles in regulation of cell volume and differentiation, vascular tone, and in hormonal secretion. SACs highly present in Xenopus oocytes and Ascidian oocytes are suggested to be involved in the regulation of pH and fluid transport to balance the osmotic pressure, but remain unclear in mammanlian oocytes. This study was investigated to find the presence of SACs in hamster oocytes and to examine their electrophysiological properties. To infer a role of SAC in relation to the development of early stage, we followed up to the stage of two-cell zygote with patch clamp techniques. Single channels were elicited by negative pressure (lower than ­15 cm$H_2O$). Interestingly, SACs were dependent on permeable cations such as $Na^+$ or $K^+$. As permeable cation removed from both sides across the membrane, SAC activity completely disappeared. When permeable cations present only in intracellular compartment, outward currents appeared at positive potentials. In contrast to this, inward currents occurred only at the negative voltage when permeable cation absent in cell interior. These result suggests that SAC carry cations through the nonselective cation channel (NSC channel). Taken together, we found that stretch activated channels present in hamster oocyte and the channel may carry cations through NSC channels. This stretch activated-NSC channels may play physiological role(s) in oocyte growth, maturation, fertilization and embryogenesis in fertilized oocytes to two-cell zygotes of hamster.

A Study on Fast Iris Detection for Iris Recognition in Mobile Phone (휴대폰에서의 홍채인식을 위한 고속 홍채검출에 관한 연구)

  • Park Hyun-Ae;Park Kang-Ryoung
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.43 no.2 s.308
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    • pp.19-29
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    • 2006
  • As the security of personal information is becoming more important in mobile phones, we are starting to apply iris recognition technology to these devices. In conventional iris recognition, magnified iris images are required. For that, it has been necessary to use large magnified zoom & focus lens camera to capture images, but due to the requirement about low size and cost of mobile phones, the zoom & focus lens are difficult to be used. However, with rapid developments and multimedia convergence trends in mobile phones, more and more companies have built mega-pixel cameras into their mobile phones. These devices make it possible to capture a magnified iris image without zoom & focus lens. Although facial images are captured far away from the user using a mega-pixel camera, the captured iris region possesses sufficient pixel information for iris recognition. However, in this case, the eye region should be detected for accurate iris recognition in facial images. So, we propose a new fast iris detection method, which is appropriate for mobile phones based on corneal specular reflection. To detect specular reflection robustly, we propose the theoretical background of estimating the size and brightness of specular reflection based on eye, camera and illuminator models. In addition, we use the successive On/Off scheme of the illuminator to detect the optical/motion blurring and sunlight effect on input image. Experimental results show that total processing time(detecting iris region) is on average 65ms on a Samsung SCH-S2300 (with 150MHz ARM 9 CPU) mobile phone. The rate of correct iris detection is 99% (about indoor images) and 98.5% (about outdoor images).

Electrochemical Properties of Pyrolytic Carbon and Boron-doped Carbon for Anode Materials In Li-ion Secondary Batteries (리튬 이온 이차전지 부극용 열분해 탄소 및 붕소첨가 탄소의 전기화학적 특성)

  • Kwon, Ik-Hyun;Song, Myoung-Youp;Bang, Eui-Yong;Han, Young-Soo;Kim, Ki-Tae;Lee, Jai-Young
    • Journal of the Korean Electrochemical Society
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    • v.5 no.1
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    • pp.30-38
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    • 2002
  • Disordered carbon and boron-substituted disordered carbons $C_{l-x}B_x(x=0.05,\;0.10,\;0.20)$ were synthesized by Pyrolysis of LPG(liquid Propane gas)and $BCl_3$. Their electrochemical properties as anode materials for Li-ion secondary batteries were then investigated. When PVDF is added to the sample in a weight ratio 5 : 95, the disordered carbon with x=0.00 had the first discharge capacity 374 mAh/g. Its cycling performance was relatively good from the second cycle and it had the discharge capacity 258 mAh/g at the 10th cycle. When PVDF is added to the sample in a weight ratio 5 : 95, the sample with x=0.05 among the samples $C_{l-x}B_x(x=0.05,\;0.10,\;0.20)$ exhibited the largest first discharge capacity 860 mAh/g and discharge capacity 181 mAh/g at the 10th cycle. All the samples had similar cycling performances from the second cycle. The sample $C_{0.90}B_{0.10}$ showed the best electrochemical properties as a anode materials fur Li-ion secondary battery from the view points of the first discharge capacity(853 mAh/g when $10w1.\%$ PVDF is used), cycling performance, discharge capacity(400mAh/g at the 10th cycle when $10wt.\%$ PVDF is used). All the samples showed generally larger charge and discharge capacities when $10wt.\%$ PVDF ratter than $5wt.\%$ PVDF is used. The plateau region in the range of voltage lower than 1.25V becomes larger probably since the structure becomes less disordered by the addition of boron. When boron is added, the charge and discharge capacities decreased suddenly at the second cycle. This may be become only a part of Li are reversibly deintercalated and intercalated and a part of Li which are strongly combined with B are not deintercalated. The increases in charge and discharge capacities are considered to be resulted from the increase in the potential of Li in the boron-added carbons, caused by the strengthening of the chemical bond between the intercalated Li and the boron-carbon host since the boron acts as electron acceptor.

Electrochemical Characteristics of LiNi0.5Mn1.5O4 Spinel as 5 V Class Cathode Material for Lithium Secondary Batteries (5V급 고전압 양극 LiNi0.5Mn1.5O4 Spinel의 제조와 전기화학적 특성에 관한 연구)

  • Jeon, Sang-Hoon;Oh, Si-Hyoung;Lee, Byung-Jo;Cho, Won-Il;Cho, Byung-Won
    • Journal of the Korean Electrochemical Society
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    • v.8 no.4
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    • pp.172-176
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    • 2005
  • Recently, many researches on the high-voltage 5 V class cathode material have focused on $LiNi_{0.5}Mn_{1.5}O_4$, where $Mn^{3+}$ in the existing $LiMn_2O_4 (Li[Mn^{3+}][Mn^{4+}]O_4)$ is replaced by $Ni^{2+}(Li[Ni^{2+}]_{0.5}[Mn^{4+}]_{1.5}O_4)$ in order to utilize $Ni^{2+}/Ni^{4+}$ redox reaction in the 5V region. The partial substitution of Mn in $LiMn_2O_4$ for other transition metal element, $LiM_yMn_{1-y}O_4$(M=Cr, Al, Ni, Fe, Co, Cu, Ga etc) is known as a good solution to overcome the problems associated with $LiMn_2O_4$ like the gradual capacity fading. In this study, we synthesized $LiNi_{0.5}Mn_{1.5}O_4$ through a mechanochemical process and investigated its morphological, crystallographic and electrochemical characteristics. The results showed that 4 V peaks had been found in the cyclic volammograms of the synthesized powders due to the existence of $Mn^{3+}$ from the incomplete substitution of $Ni^{2+}$ for $Mn^{3+}$ implying that the mechanochemical activation alone was not good enough to synthesize an exact stoichiometric compound of $LiNi_{0.5}Mn_{1.5}O_4$. The synthetic condition of mechanochemical process, such as type of starting materials, ball-mill and calcination condition was optimized for the best electrochemical performance.

Low price type inspection and monitoring system of lithium ion batteries for hybrid vessels (하이브리드 선박용 리튬 배터리의 저가형 감시시스템 구현)

  • Kwon, Hyuk-joo;Kim, Min-kwon;Lee, Sung-geun
    • Journal of Advanced Marine Engineering and Technology
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    • v.40 no.1
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    • pp.28-33
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    • 2016
  • Batteries are used for main power engine in the fields such as mobiles, electric vehicles and unmanned submarines, for starter and lamp driver in general automotive, for emergency electric source in ship. These days, lead-acid and the lithium ion batteries are increasingly used in the fields of the secondary battery, and the lead-acid battery has a low price and safety comparatively, The lithium ion battery has a high energy density, excellent output characteristics and long life, whereas it has the risk of explosion by reacting with moisture in the air. But Recently, due to the development of waterproof, fireproof, dustproof technology, lithium batteries are widely used, particularly, because their usages are getting wider enough to be used as a power source for hybrid ship and electric propulsion ship, it is necessary to manage more strictly. Hybrid ship has power supply units connected to the packets to produce more than 500kWh large power source, and therefore, A number of the communication modules and wires need to implement the wire inspection and monitor system(WIIMS) that allows monitoring server to transmit detecting voltage, current and temperature data, which is required for the management of the batteries. This paper implements a low price type wireless inspection and monitoring system(WILIMS) of the lithium ion battery for hybrid vessels using BLE wireless communication modules and power line modem( PLM), which have the advantages of low price, no electric lines compared to serial communication inspection systems(SCIS). There are state of charge(SOC), state of health(SOH) in inspection parts of batteries, and proposed system will be able to prevent safety accidents because it allows us to predict life time and make a preventive maintenance by checking them at regular intervals.

The Evaluation of the Radiation Dose and Image Quality Through the Change of the Tube Voltage in Cerebral CT Angiography (전산화단층촬영장치를 이용한 뇌 혈관조영 검사에서 관전압 변화에 따른 방사선량과 영상의 질 평가)

  • LEE, Ji-Won;Jung, Kang-Kyo;Cho, Pyong-Kon
    • Journal of radiological science and technology
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    • v.38 no.2
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    • pp.121-126
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    • 2015
  • To image diagnosis in neurovascular diseases using Multi-Detector Computed Tomography(MDCT), injected the same contrast material when inspecting Brain Computed Tomography Angiography(BCTA) to examine radiation dose and Image quality on changing Cerebral Artery CT number by tube voltage. Executed an examination with same condition[Beam Collimation $128{\times}0.6mm$, Pitch 0.6, Rotation Time 0.5s, Slice Thickness 5.0mm, Increment 5.0mm, Delay Time 3.0sec, Care Dose 4D(Demension ; D)] except for tube voltage on 50 call patients for BCTA and divided them into two groups (25 people for a group, group A: 80, group B: 120kVp). From all the acquired images, set a ROI(Region of Interest) on four spots such as left cerebral artery, right cerebral artery, posterior cerebral artery and cerebral parenchyma to compare quantitative evaluation, qualitative evaluation and effective dose after measuring CT number value from Picture Archiving Communications System(PACS). Evaluating images with CT number acquired from BCTA examination, images with 80 kVp was 18% higher in Signal to Noise Ratio and 19% in Contrast to Noise Ratio than those with 120 kVp. It was seen that expose dose was decreased by over 50% with tube voltage 80 kVp than with 120 kVp. Group A (25 patients) was examination with tube voltage 80kVp while group B with 120 kVp to examine radiation dose and Image quality. It is considered effective to inspect with lower tube voltage than with conventional high kVp, which can reduce radiation dose without any affect on diagnosis.

Extracellular $K^+$ Effects on the Mouse Aortic Endothelial Cell Contractility (쥐 대동맥 혈관 내피세포에서 세포 외 $K^+$에 의한 혈관 수축선 조절 기전)

  • 안재호;유지영
    • Journal of Chest Surgery
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    • v.36 no.12
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    • pp.887-893
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    • 2003
  • External stimuli increases intracellular (IC) $Ca^{2+}$, which increases extracellular (EC) $K^{+}$. To verify $K^{+}$ effects on the vascular contraction, we performed an experiment using mouse aortic endothelial cell. Meterial and Method: We examined the mouse aortic contractility changes as we measured the IC $Ca^{2+}$ change and ionic current by using the voltage clamp technique under different conditions such as: increasing EC $K^{+}$, removing endothelial cell, giving L-NAME (N-nitro-L-arginine methyl ester) which suppress nitric oxide formation, Ouabain which control N $a^{+}$ - $K^{+}$ pump and N $i^{2+}$ which repress N $a^{+}$-C $a^{2+}$ exchanger Result: When we increased EC $K^{+}$ from 6 to 12 mM, there was no change in aortic contractility. Aorta contracted with more than 12 mM of EC $K^{+}$. Ace-tylcholine (ACh) induced relaxation was inhibited with EC $K^{+}$ from 6 to 12 mM, but was not found after de-endothelialization or L-NAME treatment. ATP or ACh increased IC $Ca^{2+}$ in cultured endothelium. After maximal increase of IC $Ca^{2+}$, increasing EC $K^{+}$ from 6 to 12 mM made IC $Ca^{2+}$ decrease and re-decreasing EC $K^{+}$ to 6 mM made IC $Ca^{2+}$ increase. Ouabain and N $i^{2+}$ masked the inhibitory effect of endothelium dependent relaxation by increased EC $K^{+}$. Conclusion: These data indicate that increase in EC $K^{+}$ relaxes vascular smooth muscle and reduces $Ca^{2+}$ in the endothelial cells which inhibit endothelium dependent relaxation. This inhibitory mechanism may be due to the activation of N $a^{+}$- $K^{+}$ pump and N $a^{+}$-C $a^{2+}$ exchanger. $a^{+}$-C $a^{2+}$ exchanger.r.

Studies on Electrostatic Propensity of Fabrics (직물대전성에 관한 연구)

  • 최병희;배도규
    • Journal of Sericultural and Entomological Science
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    • v.27 no.2
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    • pp.54-63
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    • 1985
  • This studies has been carried out how to effect on electrostafic propensity of synthetic fabrics by coating with 0.5% acrylic polymer solution which was previously developed by the author to improve anticrease nature of silk. The work conditions are: (A) Applied synthetic polymer was acrylic polymer 525, developed by the author. (B) Electrostatic voltage for various fabrics were carried out by Korea standard abrasion partner with Korea standard (KS K 0905) cotton, nylon, polyester and the self sample fabric. (C) Applied fabrics for the investigations were carried out by using abrasion partner with Korea standard (KS K 0905) cotton, nylon, polyester and the self sample fabric. (D) Electrostatic propensity investigations were carried out by use of sample as silk, nylon, polyester and acrylic fabrics, seperating before finish or after finish. (E) Washing after the finish or the original fabric was carried out by Korea standard method, KS K 0465. Through the investigations, he happened to find many interesting matters and the obtained results are as followings. 1. Electrostatic voltage for the finished fabrics increased more than their original silk, nylon, acrylic fabrics except polyester fabric. (See Table 5) 2. Electrostatic voltage for the finished polyester against K.S. polyester decreased remarkably than the original fabric test. 3. In spite of no problem on electrostatic propensity of silk, it showed high electrostatic voltage between the same nature fabric abrasion, because silk is very weak against abrasion and because the test method had been developed to be useful for only synthetic fabrics. 4. Electrostatic voltage increased more in case of abrasion between different nature of fabrics than the same nature of fabrics. 5. Electrostatic voltage of each fabric increased by repeat of wash. 6. Many investigation data were followed with Contact Electrification Series Principle, another word, the farther each other located fabric on the series abrasion was, the higher electrostatic voltage. (See Fig. 6) 7. Such investigation gives warning of use on the mix fiber spinning service as far as concern with electrification. 8. It may also call attention for such increase of electrification in case any finishing of silk textile.

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The Heat Transfer Performance of a Heat Pipe for Medium-temperature Solar Thermal Storage System (중온 태양열 축열조용 히트파이프의 열이송 성능)

  • Park, Min Kyu;Lee, Jung Ryun;Boo, Joon Hong
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.69-69
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    • 2011
  • 태양열 발전 플랜트에 사용되는 중고온 범위의 축열조에 고체-액체간 상변화를 수행하는 용융염을 축열물질로 사용하면 액체상 또는 고체상만으로 된 열저장 매체에 비해 축열조의 규모를 축소함과 동시에 축열온도의 균일성 향상에 기여할 수 있다. 중온인 $250{\sim}400^{\circ}C$ 범위에서 이용 가능한 용융염으로는 질산칼륨($KNO_3$), 질산리튬($LiNO_3$)등이 있다. 그러나 이러한 용융염의 가장 큰 단점은 열전도율이 매우 낮다는 것이며, 이로 인해 요구되는 열전달률을 성취하기 위해서는 많은 열접촉면적이 필요하다는 것이다. 이러한 단점을 극복하는 방법을 도입하지 않고서는 축열시스템의 소규화를 성취하는데 큰 효과를 가져올 수 없다. 한편 열수송 성능이 탁월한 히트파이프를 사용하면 열원 및 열침과 축열물질 사이의 열전달 효율을 증가시켜 시스템의 성능 향상과 동시에 소규모화에 기여할 수 있다. 중온 범위 히트파이프의 작동유체로서 다우섬-A(Dowtherm-A)는 $150^{\circ}C$이상 $400^{\circ}C$까지의 범위에서 소수에 불과한 선택적 대안 중 하나이다. 따라서 본 연구에서는 용융염을 사용하는 중온 태양열축열조에 적용 가능한 다우섬-A 히트파이프의 성능을 파악하여 기술적 자료를 제시하고자 하였다. 열원으로는 고온 고압의 과열증기, 그리고 열침으로는 중온의 포화증기를 고려하였다. 용융염 축열조를 수직으로 관통하는 히트파이프는 하단부에서 열원 증기와 열교환 가능하며, 중앙부에서 축열물질과 열교환하고, 상단부에서는 중온 증기와 접촉할 수 있도록 배치하였다. 축열모드에서는 히트파이프의 하단부가 증발부로 작동하고, 중앙부가 응축부로 작동하여 용융염으로 열을 방출하면 용융염의 온도가 상승하고 용융점에 도달하면 액상으로의 상변화가 진행되면서 축열이 활성화된다. 축열모드에서 히트파이프의 상단부는 단열부로 작동한다. 방열과정에서는 히트파이프의 하단부가 단열된 상태이고, 중앙부는 용융염으로부터 열을 받아 증발부로 작동하며, 상단부는 중온 증기로 열을 방출하므로 응축부로 작동한다. 즉, 축열시스템의 작동모드에 따라 하나의 히트파이프에서 증발부, 응축부, 단열부의 위치가 변하게 된다. 특히, 히트파이프의 중앙 부분이 응축부에서 증발부로 전환될 때에도 작동이 보장되려면 내부 작동유체의 연속적인 재순환이 가능해야 하므로, 일반 히트파이프에서와는 달리 초기 작동액체의 충전량을 증발부 전체의 체적보다 더 많이 과충전해야 한다. 이러한 히트파이프의 성능 파악을 위한 실험에서 고려한 변수들은 열부하, 작동액체의 충전률, 작동온도 등이며, 열수송 성능의 지표로서는 유효열전도율과 열저항을 이용하였다. 중온범위에서 적정한 작동온도를 성취하기 위해 실험에서는 전압 조절기로 열부하를 조절하는 동시에 항온조로 응축부의 냉각수 입구 온도를 제어하였다. 하나의 히트파이프에 대해서 최대 1 kW까지의 열부하에서 냉각수 입구 온도를 $40^{\circ}C$에서 $80^{\circ}C$ 범위로 변화시키면 히트파이프 작동온도를 약 $250^{\circ}C$ 내외로 조절 가능하였다. 히트파이프 작동액체 충전률은 윅구조물의 공극 체적을 기준으로 372%에서 420%까지 변화 시켰다. 실험 결과를 토대로 열저항과 유효 열전도율을 각각 입력 열유속, 작동온도, 작동액체 충전률 등의 함수로 제시했다. 동일한 냉각수 온도에서는 충전률이 높을수록 히트파이프의 작동온도가 감소하였다. 열저항 값의 범위는 최소 $0.12^{\circ}C/W$에서 최대 $0.15^{\circ}C/W$까지로 나타났으며 유효 열전도율의 값은 최소 $7,703W/m{\cdot}K$에서 최대 $8,890W/m{\cdot}K$까지 변화했다. 최소 열저항은 충전률 420%인 경우에 나타났는데 이때의 작동온도는 약 $262^{\circ}C$이었다. 히트파이프의 작동한계로서 드라이아웃(dry-out)은 충전률 372%의 경우에 열부하 950 W에서 발생하였으나, 그 이상의 충전률에서는 열부하 1060 W까지 작동한계 발생이 관찰되지 않았다. 실험 결과 본 연구에서의 히트파이프는 중온 태양열 축열조에 적용되어 개당 약 1 kW의 열부하를 이송하면서 축열물질 및 축방열 대상 유동매체와 열교환을 하는데 사용하는데 충분할 것이라 판단된다.

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Factors Affecting Electrofusion of Plant Protoplasts (식물 Protoplast의 전기자극 융합에 관여하는 인자)

  • Han, Sung-Kyu;U, Zang-Kual;Kang, Soon-Suon;Riu, Key-Zung;Oh, Sung-Gug
    • Applied Biological Chemistry
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    • v.33 no.1
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    • pp.93-100
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    • 1990
  • The optimum conditions of electric stimulation for electrofusion of protoplasts of petunia, carrot and soybean, and the effects of calcium, magnesium, protease, trypsin, triton X-100, concanavalin A, dimethyl sulfoxide(DMSO), glycerol monooleate and spermine on fusion frequency and/or viability of petunia protoplast were investigated. The optimum frequencies(Hz)-amplitudes(V/cm) of AC Pulse for protoplast pearl-chain formation were 10 kHz-20 V/cm and 1 MHz-60 V/cm for petunia, 100 kHz-40 V/cm and $1\;MHz-40{\sim}60\;V/cm$ for carrot, and $1\;MHz-40{\sim}80\;V/cm$ for soybean, respectively. The optimum condition of DC pulse treatment at the 1 MHz-60 V/cm-15sec treatment of AC for electrofusion of petunia protoplasts was 2.5 kV/cm-40 sec, and under this condition the fusion frequency and viability of protoplasts were 45 % and 10 %, respectively, Both of the protoplasts of carrot and soybean were not fused under the AC and DC conditions tested in this experiment. The electrofusion of petunia protoplasts was stimulated by calcium, and the fusion frequency and the viability of the protoplasts were 43 % and 11 % , respectively at the calcium concentration of 140 mM. Although fusion frequency was not affected by magnesium only, magnesium stimulated fusion frequency in the presence of calcium, and the viability and fusion frequency of petunia protoplasts were 45 % and 13 %, respectively, at 140 mM of magnesium-140 mM of calcium. The relative fusion frequencies of petunia protoplasts to the controls were increased by 2.4, 2.1, 1.6, 1.4, 1.8, 1.5 and 2.2 folds, respectively, by the treatments of protease, trypsin, triton X-100, concanavalin A, DMSO, glycerol monooleate, and spermine. The viabilities of petunia protoplasts were decreased by these substances.

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