• 대한한방내과학회지
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• 제19권2호
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• pp.208-218
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• 1998

To clarify the activating effects of Buthus martensi Karsch on immunological function, its effect on primary and secondary antibodies production in mice of various ages was investigated. Buthus martensi Karsch increased the number of both antibody producing cells(anti-IgM and anti-IgG producing plaque forming cells, PFC) and phagocytic activity of peritoneal macrophage. Futhermore, these phenomena were significantly increased with aging in mice. Buthus martensi Karsch also increased natural killer cell activity concerning to cancer immunology. These results suggest that Buthus martensi Karsch markedly increases the reduced activity in the elderly and activates the immune response in senescence mice.

• 제어로봇시스템학회논문지
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• 제18권10호
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• pp.947-954
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• 2012

Sand scorpions are nocturnal animals to mostly use tactile senses to detect their prey. It has been reported that sand scorpions have high vibration sensitivity for their prey-localizing behavior. We tested vibration experiments in the sand with microphone sensors to model the sand scorpion's behavior and a time-difference model was applied to find the direction of a vibration source. Using the information of the arrival time of the vibration signal to reach each leg position, we can find the location of the vibration source.

• 헤리티지:역사와 과학
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• 제20권
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• pp.239-278
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• 1987

보성군에서 수집한 민요들의 의식·노동·유희요(謠) 별(別)로 정리하였다. 의식요(儀式謠)로는 걸메당·상여·독경(讀經)소리가 실렸다. 전래(傳來) 혼인식(式)에 있어서의 가마소리인 걸메당소리는 전국의 몇 곳에서만이 가창자가 남아 있는 희요(稀謠)이다. 상여소리(처음에 운구 들어 울릴때의 긴소리, 길 갈 때의 가난보살과 어가리넘자, 비탈길 오를 때의 나무아미타불, 달구소리)는 전남지방에 널리 전파되어 있는 것과 동계(同系)이다. 노동요(謠)는 주로 농요(農謠)<광의(廣義)>가 수집되었다. 소동패전갈을 수록하였다. 모심기곡(曲)은 공(共)히 일반 농부가 쪼임에 비하여 모찌기와 논매기곡(曲)(<표2>참조)은 다양성을 띄웠다. 산아지로고나곡(曲)은 전(全) 면(面)에 두루 퍼져 있으며 논맬 때, 장원질소리, 소동패노래에도 쓰인다. 산떨이(올라간다)는 고흥군의 것과 같다. 터 다지는 노래로는 얼럴럴 상사도야를, 상량노래로는 어유아 상량이야를 부른다. 줄메고 나가는 노래의 받음구는 위야허허 또는 상사뒤여뒤여(회촌면)이다. 유희요(謠)는 남사당의 덜미 (꼭두각시극)노래인 떼루와 제나난실을 싣고 그 위에 치마·줌치·사위·강도령·이노래 등을 실었다.

• 천문학회보
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• 제41권2호
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• pp.47.3-48
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• 2016

프랑스 몽티냑에 위치한 라스코 동굴에는 오늘날까지 발견된 수많은 구석기시대 동굴벽화 가운데 대표적인 그림들을 보존하고 있다. 동굴 벽화는 대략 BC 15,000년 전 것으로 연구되어 왔으며 최근 몇몇 학자들에 의해서 그 가운데 몇몇 그림들이 별자리를 그린 것이라는 이론이 제기되었다. 본 논문에서는 동굴 그림 가운데 황소 전당에 그려진 벽화를 별자리로 동정해 보았다. 그 결과 이것들이 성좌화임을 발견했다. 그림은 흥미롭게도 별자리와 암흑 성간운을 구별했는데 별자리는 윤곽선으로 그린 반면 검은 성간운은 검은 바탕의 그림으로 나타냈다. 그림은 벽화들의 특징과 구도 그리고 배열순서로 볼 때 전천 성좌도를 그린 것으로 동정되었는데 이는 당시 밤하늘에 보이는 별자리들과 암흑 성간운들의 구도와 배열의 일치에서 신뢰할 수 있었다. 벽화에는 황소자리, 플레이아데스, 오리온삼성, 오리온자리-쌍둥이자리, 사자자리-처녀자리-뱀자리, 천칭자리-사수자리-전갈자리가 그려져 있으며, 특히 은하중심의 사수자리에서 고물자리에 이르는 길다란 은하평면상의 검은 암흑성간운들의 특징적 나열을 들판을 뛰어가는 검은색 동물로 나타냈다. 척도를 감안해서 볼 때, 그림의 구도와 배열순서가 밤하늘에 보이는 것과 거의 같다 할만큼 사실적으로 그려져 있어서 구석기인들이 지적능력이 오늘날 현대인들과 다를 바 없는 수준에 이르렀다고 추정된다.

• 동의생리병리학회지
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• 제16권3호
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• pp.553-556
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• 2002

To study the effects of Scorpio on oxygen free radical-mediated damage by xanthine oxidase/hypoxanthine (XO/HX) on cultured spinal sensory neurons, in vitro assays such as MTT assay were used in cultured spinal sensory neurons derived from mice. Spinal sensory neurons were cultured in media containing various concentrations of XO/HX for 6 hours, after which the neurotoxic effect of XO/HX was measured by in vitro assay. The protective effect of the herb extract, Scorpio water extract against XO/HX-induced neurotoxicity was also examined. The results are as follows : In MTT assay, XO/HX significantly decreased the cell viability of cultured mouse spinal sensory neurons according to exposure concentration and time in these cultures. The effect of Scorpio water extract on XO/HX-induced neurotoxicity showed a quantitative increase in neurdfilament. These results suggest that XO/HX has a neurotoxic effect on cultured spinal sensory neurons from mice and that the herb extract, Scorpio water extract, was very effective in protecting XO/HX-induced neurotoxicity.

• Journal of Acupuncture Research
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• 제17권3호
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• pp.151-167
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• 2000

Objective : The aim of this study is to determine the antimutagenic effect and genetic safety of Buthus martensi Karsch aqua-acupuncture solution(BMKAS) against various chemical carcinogens. Method : Ames(Salmonella typhimurium) test and Rec assay(Bacillus subtilis) were used as indicators for DNA damage and antimutagenesis. Furthermore, the levels of umu operon expression by measuring the ${\beta}$-galactosidase activity wete monitored with the SOS umu test using S. typhimurium 1535 containing plasmid pSK1002. And the host-mediated assay was used to investigate the mutagenicity and antimutagenicity of BMKAS inducing various chemical carcinogens after the activation with in vivo metabolic systems. Results : From the results, BMKAS did not atfect DNA of S. typhimurium and B. subtilis strains and showed no mutagenicity at the all concentrations of tested solution. Furthermore BMKAS dose-dependently protected the mutagenecity by AF-2, 2-AA and B[a]P. These phenomena was also similar to that after metabolic activation of BMKAS in in vivo system. Conclusion : These results suggested that BMKAS did not show the mutagenicity and protected the mutagenesis against various chemical carcinogens by four different methods used in this study.

• 생약학회지
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• 제29권4호
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• pp.293-299
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• 1998

The effects of Buthus martensi Karsch (BMK) on natural killer (NK) cell activity in mouse spleen were studied. Water extracted solution of BMK was orally administrated to Balb/c mice for 2 weeks. Among splenic cells, T cell fractions were separated by Nylon wool column. Furthermore, NK cell purification was performed 4.5% percoll gradients methods. The cytotoxcity of NK cell to K562 cell was determined by lactic acid dehydrogenase and $[^3H]-thymidine $ incorporation methods. And the cytotoxicity of effector cell was most effectively induced in a ration of 50:1 (effector/target cell). As a result, cytotoxicity of NK cells was significantly increased compared with control group both in vivo and in vitro systems. The similar cytotoxic effect was shown in $[^3H]-thymidine $ incorporation methods. This suggests that when BMK is administrated to mice with malignant tumors, an increase in NK cell activity may occur and affect K562 tumor cells.

• 대한한방내과학회지
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• 제19권1호
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• pp.431-441
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• 1998

This study was undertaken to determine whether Buthus exract(BTE) affects Na^+-K^+-ATPase$ activity of nervous tissues. The enzym activity was measured in synaptosomal fraction prepared from rabbit brain cortex. Na^+-K^+-ATPase$ activity was inhibited by BTE over concentration range of 0.05-0.5% in a dose-dependent manner. The enzyme activity was increased by an increase in $Na^+$ concentration from 5 to 100mM, $K^+$ concentration from 0.5 to 10mM, and $Mg^{2+}$ concentration from 0.2 to 5mM. These changes in ion concentrations did not produce any effect on the inhibitory effect of BTE on $Na^+-K^+-ATPase$ activity. An increase in ATP concentration from 0.1 to 3mM caused an increase in the enzyme activity. The inhibition of the enzyme activity by BTE were not different between two ATP concentrations. A sulfhydryl group protector DTT prevented PCMB-induced inhibition of $Na^+-K^+-ATPase$ activity, but the BTE-induced inhibition was not altered by DTT. The inhibition of enzyme activity by combination of ouabain and BTE was not different from that by Buthus alone. These results suggest that Buthus exerts inhibitory effect on $Na^+-K^+-ATPase$ activity in cerebral synaptosomes, and the action mechansim is similar to that of ouabain.

• Journal of Acupuncture Research
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• 제19권2호
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• pp.177-188
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• 2002

Objective : The purpose of this study is to investigate the immunohistological effect of Buthus martensi Karsch Herbal-acupuncture in treating the arthritis, performed several experimental items : those are paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$. Methods : All the male Sprague Dawley rats used in this study were bred and maintaned in our pathogen-free rat colony and were 8 weeks of age at the start of to experiment. The experimental model of arthritis was induced by injection of $50{\mu}g/{\mu}{\ell}$ adjuvant(mineral oil mixed Mycobacterium butyricum). Buthus martensi Karsch Herbal-acupuncture was injected into ST36(足三里) of rats daily for 21 days. Immunohistological analysis was carried out to assess paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$ expression in synovial membrane and sera Buthus martensi Karsch Herbal-acupuncture injected. Results : Buthus martensi Karsch Herbal-acupuncture group showed a decrease with statistical significance, in paw edema, $IL-1{\beta}$, IL-6, IL-8, $TNF-{\alpha}$ and $PGE_2$ in synovial membrane and sera compared with control group. Conclusion : Buthus martensi Karsch Herbal-acupuncture stimulation inhibited the development of immunity to adjuvant induced arthritis in rats. Thus, Buthus martensi Karsch Herbal-acupuncture may have preventive effects on autoimmune inflammatory joint diseases as arthritis. The effect of Buthus martensi Karsch Herbal-acupuncture on the immune function and the disease activity in patients with arthritis warrants further investigation.

• 동의생리병리학회지
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• 제21권3호
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• pp.721-727
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• 2007

Scorpion (SCP) has been clinically used for the treatment of endogenous wind to relieve convulsion, clearing away toxins, resolving hard masses and removing obstruction in the collaterals to relieve pain. Recent studies showed that scorpion toxins that affect the activating mechanism of sodium channels and indian black scorpion venom induced anti-proliferative and apoptogenic activity against human leukemic cell lines U937 and K562. There is lack of studies regarding the effects of SCP on the immunological activities. The present study was conducted to evaluate the effect of SCP on the regulatory effects of cytokines and nitric oxide (NO) for the immunological activities in Raw 264.7 cells. After the treatment of SCP MeOH extract dissolved in media for 1 h prior to the addition of lipopolysaccharide (LPS: 1 ${\mu}$g/ml), cell viability was measured by MTT assay, NO production was monitored by measuring the nitrite content in culture medium. Inducible nitric oxide synthase (iNOS) was determined by immunoblot analysis, and levels of cytokine were analyzed by sandwich immunoassays. As results, SCP inhibited the production of nitrite and nitrate (0.3 and 1.0 mg/ml), iNOS and p-$I_KB_{\alpha}$ protein, tumor necrosis factor-${\alpha}$ (0.3 and 1.0 mg/ml), interleukin-1${\beta}$ (0.3 and 1.0 mg/ml) and interleukin-6 (1.0mg/ml) in Raw 264.7 cells activated with LPS. These findings suggest that SCP can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.