• Title/Summary/Keyword: 적조 발생

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Isolation of a Specific Antigen Protein on Cell Membrane of Cochlodinium polykrikoides, Red Bloom (적조생물 Cochlodinium polykrikoides의 세포표면 특이항원 단백질의 분리)

  • 김광현;한창희;이재훈;김병우;이복규
    • Microbiology and Biotechnology Letters
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    • v.30 no.4
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    • pp.320-324
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    • 2002
  • To establish a rapidly immunochemical identification on a dinoflagellate, Cochlodinium polykrikoides, a specific antigenic protein as a maker on the cell membrane was isolated. The cell membranes of C. polykrikoides and Gymnodinium sangineum were harvested by centrifugation after osmotic shock. The membrane proteins of both cells were solubilized in 50 mM Na-carbonate contained 1 mM DTT, and separated the proteins on SDS-PACE. Immune-blot on the solubilized membrane proteins of the both cells was performed with antiserum against the solubilized membrane proteins of C. polykrikoides. A 120 kDa membrane protein of C. polykrikoides had remarkablely different antigenicity from that of G. sangineum.

Modeling of Old Masonry Lining in Railroad Tunnels (철도터널내 조적식 라이닝의 모형화에 관한 연구)

  • Lee, J.S.;Shin, H.K.;Kim, M.I.
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.3 no.3
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    • pp.3-13
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    • 2001
  • The behavior of the masonry lining is studied to gain basic information on how to reinforce the masonry tunnels. Apart from the previous works on the masonry structures, the multi-course masonry structure, realistic in field condition, is considered and the constitutive relationship of the masonry is, therefore, established. The design charts of the orthotropic material properties are proposed according to the stiffness ratio and the crack initiation and subsequent propagation model is also considered to model the brittle nature of the masonry. A numerical analysis on the masonry panel is investigated to verify the proposed model and future works of the masonry lining are briefly explained.

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Estimation of Spatial Accumulation and transportation of Chl-$\alpha$ by the Numerical Modeling in Red Tide of Chinhae Bay (진해만 적조에 있어서 수치모델링에 의한 Chl-$\alpha$의 공간적 집적과 확산 평가)

  • Lee Dae-In
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.7 no.1
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    • pp.1-12
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    • 2004
  • The summer distribution of $Cha-{alpha}$ and physical processes for simulating outbreak region of red tide were estimated by the Eco-Hydrodynamic model in Chinhae Bay. As a result of simulation of surface residual currents, the southward flow come in contact with the northward flow at the inlet and western part of bay in case of windlessness and below wind velocity 2 m/sec. As wind velocity increases, the velocity and direction of currents were fairly shifted. The predicted concentration of $Cha-{alpha}$ exceeded 20 mg/㎥ in Masan and Haengam Bays, and most regions were over 10 mg/㎥, which meant the possibility of red tide outbreak. From the results of the contributed physical processes to $Cha-{alpha}$, accumulation sites were distributed at the northern part of Kadok channel, around the Chilcheon island, the western part of Kajo island and some area of Chindong Bay. On the other hand, inner parts of the study area such as Masan Bay were estimated as the sites of strong algal activities. Masan and Haengam Bay are considered as the initial outbreak region of red tide by the modeling and observed data, and then red tide expanded to other areas such as physical accumulation region and western inner bay, as depending on environmental variation. The increase of wind velocity led to decrease of $Cha-{alpha}$ and enlargement of accumulation region. The variation of intensity of radiation and sunshine duration caused to rapidly fluctuation of $Cha-{alpha}$: however, it was not largely affected by the variation of pollutant loads from the land only.

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Dynamic Response of Unreinforced Masonry Building (비보강 조적조의 동적 거동)

  • Kim, Nam-Hee;Kim, Jae-Kwan
    • Journal of the Earthquake Engineering Society of Korea
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    • v.8 no.5 s.39
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    • pp.1-14
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    • 2004
  • The seismic behavior of a 1/3-scale model of a two-story unreinforced masonry (URM) structure typically used in constructing low-rise residential buildings in Korea is studied through a shaking table test. The purposes of this study are to investigate seismic behavior and damage patterns of the URM structure that was not engineered against seismic loading and to provide its experimental test results. The test structure was symmetric about the transverse axis but asymmetric to some degrees about longitudinal axis and had a relatively strong diaphragm of concrete slab. The test structure was subjected to a series of differentlevels of earthquake shakings that were applied along the longitudinal direction. The measured dynamic response of the test structure was analyzed in terms of various global parameters (i.e., floor accelerations, base shear, floor displacements and storydrift, and torsional displacements) and correlated with the input table motion. Moreover, different levels of seismic performance were suggested for performance-based design approach. The results of the shaking table test revealed that the shear failure was dominant on a weak side of the 1stfloor while the upper part of the test model remained as a rigid body. Also, it was found that substantial strength and deformation capacity existed after cracking.

Bluefin tuna (Thunnus thynnus L.) Aquaculture in Yokjido, Tongyeong : Fluctuation of Phytoplankton and Reasonable Sinking Depth in Floating Cage (참다랑어 양식 : 욕지도 식물플랑크톤 군집구조 및 부침식 가두리 적정 침강수심)

  • Cho, Eun-Seob;Hwang, Hyung-Kyu
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.16 no.4
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    • pp.381-386
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    • 2010
  • We have cultured the bluefin tuna in Yokjido, Tongyeong with floating cage which was vulnerable to harmful dinoflagellate, Cochiodinium polykrikoides. This study inspected a vertical migration of phytoplankton and Chl-a for reasonable sinking depth in floating cage. Furthermore, we analyzed the fluctuation of the phytoplankton including harmful dinoflagellates occurring in Yokgido for 6 years. Total cell density showed a significant monthly variation and the flora was predominated with diatoms. Gymnodinium sp. occurred 19 times, the greatest number of occurrence in all kinds of dinoflagellates during summer. In particular, the total number of occurrence at C. polykrikoides was 8, and harmful dinoflagellates such as Karenia brevis/Fibrcapsa japonica occurred. The relationship between Chl-a concentration/total cell number and sunset/sunrise was significant and reasonable sinking depth in floating cage was found to be at least 3m from the water surface, which was associated with massive fish kills caused by C. polykrikoides.

An Experimental Study on Seismic Capacity Improvement of Masonry Buildings by Glass Fiber Reinforced Methods (유리섬유보강에 의한 조적조 건축물의 내진 성능향상에 관한 실험 연구)

  • Cho, Sang-Min;Choi, Sung-Mo;Kwon, Ki-Hyuk;Lee, Su-Cheul
    • Journal of the Korean Society of Hazard Mitigation
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    • v.4 no.2 s.13
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    • pp.47-52
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    • 2004
  • Whereas The masonry buildings are safe under gravity loads, most of the masonry buildings in Korea have many structural defects under a lateral load due to an earthquake acceleration. But there is no earthquake resistant design code for the Masonry in Korea. Therefore it may be necessary to be set up an seismic code and be suggested for reinforcing methods for existing masonry buildings. The purpose of this paper is to investigate seismic capacity of reinforced masonry buildings subjected to earthquake load. The typical two models of the masonry building in Korea are selected through a site investigation. On the basis of test results, the fiber reinforcing effect of the two models was considerable. The maximum base shear force and deformation capacity for RM were remarkably increased. It was found that the pier rocking failure was a dominant mode for the RM buildings during a seismic excitation.

An Experimental Study on Strength and Ductility of Masonry Buildings Retrofitted by Metal Connectors (조적조 건물의 연결철물보강에 따른 내력 및 연성에 관한 실험적 연구)

  • Park, Byung-Tae;Kwon, Ki-Hyuk
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.19 no.3
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    • pp.113-121
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    • 2015
  • Building using masonry accounts for most of the smaller houses in Korea but due to brittle behavior and low ductility the frequency of usage has decreased in recent years. Despite this, this form of building has been gaining attention overseas for its low cost in construction and environment-friendliness of the materials. As such, many studies are being conducted to resolve the disadvantages in structure. This study produced an specimen for masonry-filled wall and the intersection to confirm the difference in structural movement depends on the existence or lack of expansion joint and verified the reinforcement effect from inserting a connecting steel item (steel plate, stainless steel twist bar). The experiment results show that the specimen with a steel plate inserted saw an increase in durability and an improvement in the strength of the specimens, while the specimen that had stainless steel twists bar inserted saw an increase in ductility that did not cause brittle failure, indicating that the reinforcement effects of inserting a connecting steel item are effective.