• Title/Summary/Keyword: 저온성미생물

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A Field Study on the Enhancement of Landfarming Performance Using Oil-degradable Microbes Adapted to Various Temperature Range (생장 온도 범위별 최적의 유류분해 미생물을 이용한 토양경작 정화기술의 효율성 제고에 관한 현장 적용성 연구)

  • Yu, Jae-Bong;Kim, Jeong-Ho;Kim, Guk-Jin;Oh, Seung-Taek;Lee, Cheol-Hyo;Park, I-Kyong;Chang, Yoon-Young
    • Journal of Soil and Groundwater Environment
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    • v.14 no.5
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    • pp.10-17
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    • 2009
  • Bioremediation has been applied as a proven technology in remediation of TPH contaminated soil. However, the efficiency of biodegradation is dependent on temperature as microbial activity is depressed at lower temperature ranges ($30^{\circ}C{\sim}80^{\circ}C$). The objective of this study was to develop microbes with enhanced activities at the stated temperature conditions and to evaluate the remediation effectiveness of these microbes in TPH contaminated soil. Experiments were conducted to isolate hydrocarbon degradable microbial consortia cultured under different temperature conditions. It was found that there were 5 strains of mesophilic ($30^{\circ}C$) and 3 strains of psychrophilic ($80^{\circ}C$) microbes. The TPH concentration was reduced from 4,044 mg/kg to 1,084 mg/kg, (73.2%) in 10 days by using mesophilic microbial consortia and from 5,427 mg/kg to 1,756 (67.6%) in 50 days with psychrophilic microbial consortia in laboratory cultures under controlled conditions. This rate determination excluded physical degradation such as venting and dilution. A field study was then performed to examine the feasibility of applying these microbes in the land-farming process. In this case, 87.1% of the 2,560 mg/kg TPH contaminated soil was degraded in 56 days. The biodegradation rate coefficient (k) was $0.0374\;day^{-1}$. Findings of this study provide viable options for applying microbes for bioremediation of TPH in lower temperature conditions.

Characterization of Extracellular Proteolytic Enzyme of Isolated Psychrotrophic Bacteria from Cheddar Cheese (체다치즈에서 분리한 내냉성미생물의 단백질분해효소의 특성)

  • Kim, Eun-Ah;Lee, Kyung-Wook;Boo, Won-Back;Lee, Hyung-Hoan;Kwak, Hae-Soo
    • Korean Journal of Food Science and Technology
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    • v.23 no.4
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    • pp.452-458
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    • 1991
  • Psychrotrophs producing protease were isolated during ripening periods of Cheddar cheese and one of them containing the highest protease activity was identified as Pseudomonas fluorescens 65. The extracelluar proteolytic enzyme was partially purified from P. fluorescens 65 through the Sephadex G-100 gel filtration. The protease was eluted between 190 ml and 230 ml of elution volume of sodium phosphate buffer. The purified protease showed a single band in SDS-PAGE and its molecular weight was 47,000. The composition of amino acid for the protease was determined and the most abundant amino acids were glutamic acid (14.96%) and serine (13.86%). The optimum temperature and pH for the activity was $45{\sim}50^{\circ}C$ and 6.0, respectively.

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