• Title/Summary/Keyword: 재생제

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Effects of mechanical stress and interleukin-$1{\beta}$ on collagenase and TIMP-1 expression in human periodontal ligament fibroblasts (기계적 자극과 interleukin-$1{\beta}$가 치주인대 섬유아세포의 collagenase와 TIMP-1의 발현에 미치는 영향)

  • Kim, Myung-Lip;Bae, Chang
    • The korean journal of orthodontics
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    • v.28 no.1 s.66
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    • pp.165-174
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    • 1998
  • The turnover of collagen is controlled by the balance between collagen synthesis and degradation. The production of collagenase (matrix metalloproteinase-1) and its inhibitor, tissue inhibitor of matrix metallopmteinase-1 (TIMP-1) are one of the substances which regulate this balance. The periodontal ligament fibroblast plays an important role in collagen metabolism during orthodontic treatment and is believed to be an origin of the osteoblast in the alveolar bone. The collagenase secreted by the periodontal ligament fibroblast and the osteoblast initiates the bone resorption by removing the osteoid layer in the alveloar bone. The interleukin-$1{\beta}$ is secreted by the macrophage during orthodontic treatment. The present study was undertaken to assess the effect of mechanical stress and interleukin-$1{\beta}$ on the expression of collagenase and TIMP-1 in the periodontal ligament fibroblasts using reverse transcription polymerase chain reaction and immunohistochemical staining. The periodontal ligament fibroblasts were stitched by placing the $Petriperm dish^{\circledR}$ dish on the top of spheroidal convex watch glass ($5\%$ surface increase) and tented with interleukin-$1{\beta}$ (1.0 ng/ml), or treated with both of them. Treatment with mechanical stress and/or interleukin-$1{\beta}$ resulted in increased collagenase mRNA expression. The mechanical stress treated group (1.61, 1.62, 1.37 fold increase), the interleukin-$1{\beta}$, tented group (1.68, 1.60, 3.78 fold increase), the mechanical stress and interleukin-$1{\beta}$ treated group (1.89, 1.72, 5.48 fold increase) induced increases in collagenase mRNA compared with the control group after 2, 4, 8 hours respectively. But TIMP-1 mRNA expressions at experimental groups were decreased after 2, 4 hours and increased after 8 hours. The mechanical stress treated group (0.16, 0.49 fold decrease and 3.77 fold increase), the interleukin-$1{\beta}$ treated group (0.15,0.44 fold decrease and 4.46 fold increase), the mechanical stress and interleukin-$1{\beta}$ tented group (0.15, 0.69 fold decrease and 4.81 fold increase) induced changes in TIMP-1 mRNA compared with the control group after 2, 4, 8 hours, respectively. Immunohistochemical stain showed that increased collagenase and TIMP-1 staining of the mechanical stress tented group, the interleukin-$1{\beta}$ treated group, and the mechanical stress and interleukin-$1{\beta}$ treated group compared with that of the control group after 8 hours. These findings suggest that mechanical stress and interleukin-$1{\beta}$ regulate expression of collagenase and TIMP-1.

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Studies on the Growth Characteristics, Forage Yields and Nutritive Values of Heading and Headless Types of Sorghum X Sudangrass Hybrids (출수 및 비출수형 Sorghum X Sudangrass 교잡종의 품종별 생육특성, 생산성 및 사료가치에 관한 연구)

  • 임영철;성병렬;최기준;임용우;김기용;임근발;박근제
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.3
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    • pp.213-220
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    • 2002
  • The growth characteristics, forage yields and nutritive values of heading and headless types of sorghum $\times$ sudangrass(Sorghum bicolar L.) hybrids were examined to evaluate the adaptability of new cultivars at Suwon and Chunahn for two years from 2000 to 2001 in a randomized complete block design with three replications. Eleven cultivars of heading type and 4 cultivars of headless type were used in this study. The dry matter(DM) yield of AG 2501 was highest in the heading type cultivars, and PAC F8350 in the headless type cultivars, respectively. The DM yields of heading types were slightly higher than those of headless types but there were not significantly different. Early growth and regrowth characteristics of heading type was highest in AG 2501 and GW 9110G, respectively. GW 9110G and DMD were early heading types, and AG 2501 and GW 9161F were middle heading types. AG 2501 was longest and GW 9161F was shortest in plant height. Cultivars with long leaf length were appeared to be larger fur leaf width and stem diameter. Brix content was highest in DMD as 9.7%, and disease and insect resistances were not significantly different among the heading types. Early growth of headless type cultivar, PAC F8350 was slightly lower than that of Jumbo but stronger in regrowth, wider in leaf width and stem diameter, and stronger in lodging. Comparisons of growth characters between heading and headless types of cultivars were not easy in general but between them, brix contents of heading types ranging from 6.7 to 7.9% were greatly higher than those of headless types ranging from 3.6 to 3.9%. Nutritive values such as crude protein, ADF, NDF and digestibility of headless type cultivars were higher than those of heading types. In conclusion, of heading type cultivars, GW 9110G, AG 2501, DMD and GW 9161F, and of headless type cultivars, Jumbo and PAC F8350 were recommended as high yielding cultivars with good adaptability in domestic environment.

Recovery of N and P Resources from Animal Wastewater by Struvite Crystallization (Struvite 결정화에 의한 축산폐수로 부터 질소.인 자원의 재생)

  • Jo, W.S.;Yoon, S.J.;Ra, C.S.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.875-884
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    • 2003
  • Operational parameters for struvite crystallization, as a process to recover nitrogen and phosphorus resources from animal wastewater, were studied in this research. Crystallization distinctive of NH$_4$-N and PO$_4$$^{3-}$ in accordance to chemical sources, influent pH, aeration and stirring was examined using 2L of working volume of struvite reactor. Also, to find an effective treatment process combining with electrolysis method, removal characteristics of NH$_4$-N and PO$_4$$^{3-}$ in 6 different processes was tested. As chemical sources for the derivation of struvite formation, MgSO$_4$ and MgCl$_2$ were superior to CaCO$_3$ and CaCl$_2$. From experiment which was conducted to know the effects of aeration and stirring on struvite formation, it was revealed that aeration stimulated the crystallization reaction by inducing faster pH increase. While 90% of P removal was achieved within 1 hour under aeration, 14 hours was consumed under stirring condition. Struvite formation under aeration was affected by influent pH. No crystallization was observed at pH 5 level, but active crystallization reaction was induced over pH 6.0. 95% of P removal by struvite formation at pH 6, 7 and 9 was achieved within 3h, 2h and 10 min., respectively. However, over pH 10, operational problem due to excessive foam formation occurred, and blunting of crystallization reaction was observed at pH 11. When consider the pH range of animal wastewater, pH 7 to 9, efficient struvite formation could be achieved by simple aeration, without any chemical usage for pH adjustment. Among tested processes, the treatment process which electrolyzing the supernatant from struvite reactor, providing air to both reactors, showed best pollutant removal efficiencies. In this combined process, the removal efficiencies of NH$_4$-N and PO$_4$$^{3-}$ was 86% and 98%, respectively, and 92.4% of color removal was obtained.

Sensitivity of NOx Removal on Recycled TiO2 in Cement Mortar (재생 이산화티탄을 혼입한 모르타르의 NOx 저감률 민감도 분석)

  • Rhee, Inkyu;Kim, Jin-Hee;Kim, Jong-Ho;Roh, Young-Sook
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.4
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    • pp.388-395
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    • 2016
  • This paper explores the photocatalytic sensitivity of cement mortar incorporated with recycled $TiO_2$ from waste water sludge. Basically, $TiO_2$ cluster sank down slowly to the bottom of cement mortar specimen before setting and hardening process. This leads the mismatch of $TiO_2$ concentration on the top and the bottom faces of a specimen. This poorly dispersed $TiO_2$-cement mortar naturally exhibits poor NOx removal efficiency especially on the top of cementitious structure. In architectural engineering application such as building or housing structures, one can simply filp over from the bottom so that more $TiO_2$ concentrated surface can be placed outward into the air. However, in highway pavement case, this could not be applicable due to in-situ installation of concrete pavement. Hence, the dispersion of $TiO_2$ cluster inside the cementitous material is getting important issue onto road construction application. To elaborate this issue, according to our results, silica fume, high-ranged water reducer, viscosity agent, blast furnace slag were not enhanced much of dispersion characteristics of $TiO_2$ cluster. The combination of foaming agent and accelerator of hardening with viscosity agent and small grain size of fine aggregate may help the dispersion of $TiO_2$ inside cementitious materials. Even though the enhanced dispersion were applied to the specimen, NOx removal efficiency doest not change much for the top surface of the specimen. This concurrently affected by the presence of tiny air voids and the dispersion of $TiO_2$ in that these voids could easily adsorbed NOx gas with the aid of large surface area.

Development of Potassium Impregnated Carbon Absorbents for Indoor CO2 Adsorption (K계열 함침 탄소계 흡착제의 실내 저농도 이산화탄소 흡착성능 강화)

  • Jeong, Se-Eun;Wang, Shuang;Lee, Yu-Ri;Won, Yooseob;Kim, Jae-Young;Jang, Jae Jun;Kim, Hana;Jo, Sung-ho;Park, Young Cheol;Nam, Hyungseok
    • Korean Chemical Engineering Research
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    • v.60 no.4
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    • pp.606-612
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    • 2022
  • Relatively high indoor CO2 concentration (>1,000 ppm) has a negative impact on human health. In this work, indoor CO2 adsorbent was developed by impregnating KOH or K2CO3 on commercial activated carbon, named as KOH/AC and K2CO3/AC. Commercial activated carbon (AC) showed relatively high BET surface area (929 m2/g) whereas KOH/AC and K2CO3/AC presented lower BET surface area of 13.6 m2/g and 289 m2/g. Two experimental methods of TGA (2,000 ppmCO2, weight basis) and chamber test (initial concentration: 2,000 ppmCO2, CO2 IR analyzer) were used to investigate the adsorption capacity. KOH/AC and K2CO3/AC exhibited similar adsorption capacities (145~150 mgCO2/g), higher than K2CO3/Al+Si supports adsorbent (84.1 mgCO2/gsample). Similarly, chamber test also showed similar trend. Both KOH/AC and K2CO3/AC represented higher adsorption capacities (KOH/AC: 93.5 mgCO2/g K2CO3/AC: 94.5 mgCO2/gsample) K2CO3/Al+Si supports. This is due to the KOH or K2CO3 impregnation increased alkaline active sites (chemical adsorption), which is beneficial for CO2 adsorption. In addition, the regeneration test results showed both K-based adsorbents pose a good regeneration and reusability. Finally, the current study suggested that both KOH/AC and K2CO3/AC have a great potential to be used as CO2 adsorbent for indoor CO2 adsorption.

The Effects of Platelet-Rich Fibrin on Osteoblast Proliferation and Differentiation: Effects of Platelet-Rich Fibrin on Osteoblasts (혈소판 농축 섬유소가 골모세포 증식과 분화에 미치는 영향)

  • Jung, Hae-Su;Bae, Hyun-Sook;Hong, Ki Seok
    • Journal of dental hygiene science
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    • v.13 no.2
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    • pp.158-164
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    • 2013
  • The most frequently encountered problems at fixture-implantation sites are lack of adequate bone and proximity to anatomic structures. It is generally accepted that growth factors play an essential role in the healing process and tissue formation, and they have become the focus of grafting materials research. The granules in platelets contain high concentrations of various growth factors. In particular, platelet-rich fibrin (PRF) is a second-generation platelet concentrate that allows the production of fibrin membranes enriched with platelets and growth factors from an anticoagulant-free blood harvest. This study investigated the in vitro effects of PRF on osteoblasts, in terms of the key cellular functions, and especially the effects on two growth factors, the homodimer of platelet-derived growth factor subunit B (BPDGF-BB) and transforming growth factor (TGF)-${\beta}1$, which are associated with wound healing and regeneration (i.e., proliferation and differentiation). The following parameters were investigated: PDGF-BB and TGF-${\beta}1$ levels in PRF, cell viability, alkaline phosphatase (ALP) activity, type 1 collagen synthesis, and the expressions of osteoblast differentiation markers (ALP and runt-related transcription factor 2) and bone matrix proteins (type 1 collagen). The release of autologous growth factors from PRF was maintained for a reasonable period of time, and exerted positive effects on the proliferation and differentiation of osteoblasts. The use of PRF thus appears to be a promising method for enhancing bone healing and remodeling.

The Vegetation Structure and Management Solution of the Natural Monument (No.374) Pyeongdae-ri Torreya nucifera forest of Jeju (천연기념물 제374호 제주 평대리 비자나무 숲의 식생구조 및 관리방안)

  • Lee, Won-Ho;Oh, Hae-Sung
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.31 no.3
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    • pp.54-60
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    • 2013
  • This study carried out a phytosociological research by Z.-M. school's method describing vegetation structure and management solution about nutmeg tree's(Torreya nucifera ) forests. The nutmeg tree's forests have developed intervale and hills in warm-temperate evergreen broad-leaved forests zone. The nutmeg tree's forests composed 50 familly, 80 genus, 88 species divided two syntaxa(typical sub-community, Arachniodes aristata sub-community) by species composition and correspond to habitat condition. The nutmeg tree's forests show a uniform forest's structure which composed 3~4 layer of sub-tree and shrub. And it was worthy of special mention which characterized diagnostic species of Camellietea japonicae in the warm-temperate evergreen broad-leaved forests. In case of forests floor, were a high rate in order of Ophiopogon japonicus, Polystichum tripteron, Arisaema ringens. And Oplismenus undulatifolius which represent mantle communities as well. This indicates that the nutmeg tree's forests are exposed by humman effects sustainedly. The nutmeg tree's young trees appeared a high rate at half shade and shade. The vigorous tree species have to spacing cutting instead of the heavy thinning that consist in with a shout of declining astructur and function of the forest ecosystem for maintaining a nutmeg tree's forests. And we suggest for the density regulation of the sub-tree and shrub. In conclusion, need to the continuous monitoring about the vegetation structure and management solution for the sustainable nutmeg tree's forests.

The Research for effect of lubricant oil aging on environmental performance (자동차 윤활유의 성상 및 열화가 환경성에 미치는 영향 연구)

  • Kim, Jeong-Hwan;Kim, Ki-Ho;Ha, Jong-Han;Jin, Dong-Young;Myung, Cha-Lee;Jang, Jin-Young
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.1
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    • pp.12-24
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    • 2017
  • The main purpose of this research is for the investigation on the impact of engine oil aging on PM and DPF. It is widely known that lubricant specifications and consumption from an ICE have significantly influenced on the regulated and unregulated harmful emissions as the engine operating conditions. Considering DPF clogging phenomena with lubricant-derived soot/ash components, simulated aging mode for the DPF was newly designed for engine dynamometer testing. PM/ash accumulation cycle were developed in reflecting real-world engine operating conditions for the increment of engine oil consumption and natural DPF regeneration for the ash accumulation. The test duration for DPF aging reached around 100hrs with high- and low-SAPS engine oils, respectively. Using high SAPs engine oil made more PM/ash accumulation compared with low SAPs engine oils and it could accelerate fouling of EGR in engine. Fouling of EGR made effects on more harmful exhaust gases emissions. The test results on engine lubricant under engines operating conditions will deliver for the establishment of regulated and unregulated toxic emissions policy, lubricant quality standard.

Development of Oxy-fuel Combustor for the Underwater SMV(Sub-Merged Vaporizer) (수중연소식 천연가스기화기(SMV)용 순산소 연소기 개발)

  • Sohn, Whaseung;Kim, Hoyeon;Jeong, Youngsik
    • 한국연소학회:학술대회논문집
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    • 2014.11a
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    • pp.253-254
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    • 2014
  • 지구온난화 문제는 한국가의 문제가 아니라 인류의 문제로 대두되어 많은 이에대한 많은 연구가 이루어 지고 있다. 지구온난화의 주 대상물질인 화석연료로부터 연소시 발생하는 이산화탄소를 감축하기위한 많은 규제와 노력이 요구된다. CCS(Carbon Capture & Storage)란 화석연료로 부터 연소시 대기 중으로 배출되는 온실가스($CO_2$)를 포집하여 재생 또는 지중, 해양에 저장하는 기술로서 국가녹색성장 핵심기술중의 하나로 분류되며, $CO_2$ 회수방안, 저장, 처리관련 연구를 비롯하여 국내외 적으로 활발한 연구가 이루어 지고 있다. 또한 순산소 연소기술을 통한 $CO_2$ 회수, 처리기술은 연료의 산화제를 공기대신 순도 95% 이상의 고농도 산소를 이용하여 순산소연소를 하며, 이때 발생하는 배가스의 대부분은 $CO_2$와 수증기로 구성되어 있다. 발생된 배가스의 약 70~80%를 다시 연소실로 재순환시켜 연소기의 열적 특성에 적절한 연소가 가능하도록 최적화함과 동시에 배가스의 $CO_2$ 농도를 80% 이상으로 농축시켜 회수를 용이하게 하며, 동시에 공해물질은 NOx 발생량을 10ppM 이하로 줄일 수 있는 기술이다. 천연가스를 생산하는 LNG기지에서 연소에 의한 이산화탄소를 발생시키는 기기로는 수중연소식기화기(SMV ; Submerged Combustion Vaporizer)를 들 수 있다. SMV는 버너를 이용하여 $-162^{\circ}C$ LNG를 $10^{\circ}C$의 LN로 기화시키는 설비로서 특히 동절기에 작동시키며 $CO_2$를 배출시키는 연소기다. 본 연구에서는 수중연소식 SMV에 순산소 연소방식을 적용하여 천연가스와 산소를 연소시키므로서 발생되는 $CO_2$를 LNG냉열을 이용 액체화 시켜 회수하는 연구를 수행하고 있다. 내용중에 수중연 소식 SMV에 대한 순산소 연소기를 개발하는 연구를 수행하였으며, 실제 SMV의 1/10크기, 열량기준 1/900로 모형을 제작하여 실험하였다. 연소기 노즐 은 직경 0.6mm, 배가스가 수조내에서 48개의 노즐을 제작하였다. 실험결과 일정량 이상의 $CO_2$ EGR율이 일정 값 이상이 되면 화염의 길이가 공기/NG 화염 길이와 큰 차이가 없었으며 $CO_2$ EGR율이 100%이상에서는 $CO_2$ EGR율 증가에 따른 화염길이 변화는 크게 나타나지 않았다. CO 배출 농도는 공기/NG 연소의 경우보다 높게 나타났으며, ${\lambda}$가 1.4보다 높은 조건에서는 측정되지 않았다. NOx의 배출 농도는 약 1~8ppm으로 나타났다.

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Study on free and bond glycerines in Biodiesel from PKO(Palm Kernel Oil) and coconut oil (PKO 및 코코넛유래 바이오디젤 중 글리세린함량 분석 방법 개선 연구)

  • Lee, Don-Min;Park, Chun-Kyu;Ha, Jong-Han;Lee, Bong-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.2
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    • pp.348-361
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    • 2015
  • To reduce the effects of greenhouse gas (GHG) emissions, the government has announced the special platform of technologies as parts of an effort to minimize global climate change, and the government distributed biodiesel since 2006 as the further efforts. Although there are some debates about some quality specifications and unbalanced of source (44% from palm oil), more than 400kton/year of biodiesel was produced in 2013. Moreover the amounts will be increased when the RFS is activated. To solve the unbalanced situation and to achieve the diversity of feeds, it is essential that many researches should be considered. Especially, free and bond glycerines are one of the important properties seriously affected to the combustion system in vehicle & cold properties. Previous method (KS M 2412) couldn't cover the biodiesel derived from lauric oil($C_{12:0}$) such as PKO (Palm Kernel Oil), Coconut oil because those compositions are lighter than other conventional biodiesel sources. In this study, we review the existed method and figure out the factors should improve to analysis the glycerine from PKO and Coconut oil biodiesel. Modifying the analysis conditions to enhance the resolution and change the internal standards to avoid the overlapped- peaks between Capric acid ME ($C_{10:0}$) and standard#1(1,2,4-butantriol). From this revised method, we could solve the restrictions of previous methods. And check the possibility of new method to analyze the glycerine in biodiesel regardless of sources.