• Title/Summary/Keyword: 장관 면역

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Role of Retinoic Acid in the Gut Mucosal Immunity (장관면역에 있어서 레티노인산의 역할)

  • Shon, Dong-Hwa
    • Bulletin of Food Technology
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    • v.23 no.4
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    • pp.535-543
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    • 2010
  • 장관은 우리 몸에서 가장 넓은 표면적을 가지고 있으며, 외부로부터 침입하는 이물에 대항하여 면역세포의 배치가 필수적이다. 항원과 만난 적이 없는 naive lymphocyte는 임파절 등 2차 임파기관의 수지세포(dendritic cell)가 임파구에 항원을 제시할 때 레티노인산(retinoic acid)을 생산하여 제공함으로써, 소장 조직에 특이적으로 임파구가 호밍(homing)하는 능력을 부여한다. 한편, 장에 있어서는 식품항원에 대한 면역반응을 억제할 필요가 있는데, RA는 T세포의 기능분화를 억제하여 면역관용의 성립에도 관여함이 밝혀졌다.

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Bone Marrow Cell Proliferation Activity through Intestinal Immune System by the Components of Atractylodes lancea DC. (창출 성분의 장관면역 자극을 통한 골수세포 증식활성)

  • Yu, Kwang-Won;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.33 no.1
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    • pp.135-141
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    • 2001
  • Of hot-water extracts prepared from 10 herbal components of Sip-Jeon-Dae-Bo-Tang, Atractylodes lancea DC. (ALR) and Panax ginseng C.A. Meyer (PG) showed the most potent bone marrow cell proliferation activity through intestinal immune system whereas other extracts did not have the activity except for Astragalus membranacues Bunge (ASR) and Angelica acutiloba Kitagawa (AR) having low activity. Especially, ALR had the potent activity irrespective of classes of ALR, a place of production and the condition of breeding. In addition, we found that hot-water extract from Atractylodes lancea DC rhizomes (ALR-0) contributed mainly to Peyer's patch cells mediated-hematopoietic response of Sip-Jeon-Dae-Bo-Tang. ALR-0 was further fractionated into MeOH-soluble fraction (ALR-1), MeOH-insoluble and EtOH-soluble fraction (ALR-2), and the crude polysaccharide fraction (ALR-3). Among these fractions, only ALR-3 showed potent stimulating activity for proliferation of bone marrow cells mediated by Peyer's patch cells, dose-dependently. In treatments of ALR-3 with $NaIO_4,\;NaClO_2$ and pronase, all significantly reduced the intestinal immune system modulating activity of ALR-3, and the activity of ALR-3 was much affected by $NaIO_4$ oxidation particularly. These results reveal that macromolecules, such as polysaccharide, rather than low-molecular-weight substances, are the potent intestinal immune system modulating compound of ALR.

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An Immunohistochemical study of the gastro-entero-pancreatic endocrine cells of the insectivorous Korean mole, Talpa micrura coreana (한국산 두더지의 위장췌내분비세포에 관한 면역조직학적 연구)

  • Lee, Hyeung-sik;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.36 no.4
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    • pp.747-755
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    • 1996
  • The regional distribution and relative frequencies of the gastro-entero-pancreatic endocrine cells were studied immunohistochemically in the GIT and pancreas of the Korean mole. Seven kinds of endocrine cells were identified in this study. Although 5-HT- and somatostatin-immunoreactive cells were seen throughout the GIT, they were most predominant in the pyloric gland region. Glucagon-immunoreactive cells were restricted on the large intestine. Bovine CG-immunoreactive cells were more frequent in the stomach than in the intestines which were not detected in the duodenum. Numerous Gas/CCK-immunoreactive cells were found in the pyloric gland region, but rarely in the jejunum and ileum. BPP-immunoreactive cells were observed to be rare in the stomach and ileum but were a few in number in the intestines. In the pancreas, four types, namely, glucagon-, somatostatin-, BPP- and insulin-immunoreactive cells were identified in the pancreatic islets and exocrine portion. These results suggest that although endocrine cells of the Korean mole is less abundant in the duodenum, the distribution pattern of its gastro-entero-pancreatic endocrine cells is similar to that reported for the Korean hedgehog.

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Modulation of the inflammatory process and interaction of THP-1 monocytes with intestinal epithelial cells by glasswort (Salicornia herbacea L.) extracts (인간 단핵구 THP-1의 염증반응 및 장관상피세포와의 상호작용에 미치는 퉁퉁마디 추출물 분획의 영향)

  • Choi, Yoo Mi;Kang, Smee;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.378-383
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    • 2016
  • The glasswort is an edible halophyte demonstrating various physiological effects including anti-inflammatory activity. In the present study, the effects of glasswort extracts on inflammatory events and interactions of THP-1 monocytes with intestinal epithelial cells were investigated. Five solvent fractions, including the ethylether fraction (Fr.E), were prepared from a 70% methanol extract of glasswort. THP-1 monocytes underwent differentiation by phorbol 12-myristate 13-acetate treatment and were then activated by lipopolysaccharide (LPS), which induced cyclooxygenase (COX)-2 expression. None of the glasswort fractions tested alone induced COX-2 in differentiated THP-1 cells. Fr.E, however, enhanced LPS-induced COX-2 expression in differentiated THP-1 cells. Culture media of THP-1 cells treated with each fraction stimulated the growth of normal intestinal INT-407 cells more prominently than that of HT-29 colon cancer cells. COX-2 expression in HT-29 cells was inhibited when the cells were exposed to the THP-1 culture medium treated with Fr.E. Thus, glasswort could modulate the interaction between immune cells and intestinal cells.

Activation of Intestinal Immune System by an Orally Administered Methanol Extract from Pine Needles (솔잎 메탄올추출물의 마우스 경구투여에 의한 장관면역 활성)

  • Yoon, Jin-A;Yu, Kwang-Won;Shin, Shun-Hyuck;Cho, Hong-Yeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.3
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    • pp.356-362
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    • 2010
  • When pine (Pinus densiflora) needles were fractionated into cold water (PD-CW), hot water (PD-HW) and methanol extract (PD-M), PD-M showed potent simulating activity (1.19-fold of the saline control) for proliferation of bone marrow cells mediated by Peyer's patch cells in vitro. MeOH extracts were prepared by homogenization, stirring or reflux to identify the method of methanol extraction, and MeOH extract by reflux method showed significantly highest intestinal immune system modulating activity (1.30-fold) in vitro. The intestinal immune system modulating effect of orally administered PD-M fractionated from pine needles also were studied in mice. Analyzing intestinal immune system modulating activity mediated Peyer's patch cells from C3H/He mice which had been fed with PD-M at different doses for 7 days, 1.0 g/kg of BW/day indicated that the bone marrow cells had proliferated (3.65-fold of 3% EtOH administered group). In addition, the amounts of IL-6 in the culture supernatant of Peyer's patch cells at 1.0 g/kg of BW/day were increased (1.13-fold) whereas the production of GM-CSF was not dose dependent. These results indicate that oral administration of PD-M enhances the secretion of hematopoietic growth factors such as GM-CSF and IL-6 from Peyer's patch cells, and these cytokines also act on modulator of bone marrow cell proliferation.

Immunohistochemical study on the gastro-entero-pancreatic(GEP) endocrine cells of the blue fox, Alopex lagopus (북극여우의 위장췌 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.33 no.3
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    • pp.369-379
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    • 1993
  • The regional distribution and the relative frequencies of endocrine cells were studied in nine portions of the blue fox GI tract, and the distribution pattern and cell types of the pancreatic endocrine cells were also studied in the pancreas by immunohistochemical method. Six kinds of immunoreactive cells were identified in the GI tract, and four kinds of immunoreactive cells were also identified in the pancreas. Although numerous 5-HT- and somatostatin-immunoreactive cells were seen throughtout the GI tract, somatostatin-immunoreactive cells were a few in the intestine. Very numerous Gas/CCK-immunoreactive cells were restricted generally in the pyloric region and duodenum. Numerous glucagon-immunoreactive cells were found in the stomach except the pyloric region, and generally a few in the intestine. Moderate number of BPP-immunoreactive cells were found in the stomach except the pyloric region, and a few in the large intestine. Numerous porcine CG-immunoreactive cells were restricted to the cardiac and fundic region. In the pancreas, four types of pancreatic endocrine cells-somatostatin-, glucagon-, BPP- and insuline-immunoreactive-were identified in the pancreatic islet and exocrine portion. These results suggest that the regional distribution, the relative frequencies and cell types of the GEP endocrine cells in the GI tract and pancreas varies considerably among the species.

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Immunohistochemical study on the gastro-entero-pancreatic(GEP) endocrine cells of the blue fox, Alopex lagopus (북극여우의 위장췌 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.579-589
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    • 1993
  • The regional distribution and the relative frequencies of endocrine cells were studied in nine portions of the blue fox GI tract, and the distribution pattern and cell types of the pancreativc endocrine cells were also studied in the pancreas by immunohistochemical method. Six kinds of immunoreactive cells were identified in the GI tract, and four kinds of immunoreactive cells were also identified in the pancreas. Although numerous 5-HT- and somatostatin-immunoreactive cells were seen throughout the GI tract, somatostatin- immunoreactive cells were a few in the intestine. Very numerous Gas/CCK-immunoreactive cells were restricted generally in the pyloric region and duodenum. Numerous glucagon-immunoreactive cells were found in the stomach except the pyloric region, and generally a few in the intestine. Moderate number of BPP-immunoreactive cells were found in the stomach except the pyloric region, and a few in the large intestine. Numerous porcine CG-immunoreactive cells were restricted to the cardiac and fundic region. In the pancreas, four types of pancreatic endocrine cells- somatostatin-, glucagon-, BPP- and insulin-immunoreactive- were identified in the pancreatic islet and exocrine portion. These results suggest that the regional distribution, the relative frequencies and cell types of the GEP endocrine cells in the GI tract and pancreas varies considerably among the species.

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Tissue origin of soluble component proteins in salin extract of adult Paragonimus westermani (폐흡충 성충 수용성 단백질의 성분별 생성위치)

  • Yoon Kong;Chi- Yong Park;Shin-Yong Kang;Seung-Yull Cho
    • Parasites, Hosts and Diseases
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    • v.30 no.2
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    • pp.91-100
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    • 1992
  • Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf values, strips of gel containing each band protein was cut out. Each band protein was eluted by electrophoresis. Monospecific antibodies were prepared by immunizing rabbits with each band protein. When peroxidase-antiperoxidase (PAP) staining was done, antiserum to Band 1 reacted to content of eggs both in the worm and in the infected lung tissue. Antiserum to Band 2 reacted to parenchymal tissue of the worm. Antiserum to Band 4 showed the positive reaction at intestinal content while that to Band 5 reacted to the intestinal epithelial border. Antiserum to combined proteins of Bands 617 and that to Band 8 reacted to parenchymal tissue of the worm respectively. From the results, the origin of individual proteins in crude extract of adult P. westermani could be differentiated.

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