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Electrophoretic Patterns of Hemolymph Proteins of Varieties with Long and Short Life Span in the Silkworm Bombyx mori L. (${\cdot}$단명 누에 품종의 체액단백질의 전기영동상)

  • Kang, Pil-Don;Yoon, Hyung-Joo;Ryu, Kang-Sun;Sohn, Bong-Hee;Sohn, Hung-Dae
    • Journal of Sericultural and Entomological Science
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    • v.41 no.1
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    • pp.1-8
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    • 1999
  • Hemolymph protein patterns of silkworms in terms of short and long life span were analyzed by native- and SDS-polyacrylamide gel electrophoresis (PAGE) with the developmental stages. From the native-PAGE patterns of silkworm major hemolymph proteins there were varietal differences on the first day of the pupal stage and were classified into there groups MHP-a, b and c SA 10, JAM109 and J037 were grouped into MHP-Ⅰ, Hangang, Chungmun and Daizo(sdi) into MHP-Ⅱ and NTZN, Sulak, Qoichuk and PR varieties into MHP-Ⅲ group. It was found that the MHP in each group revealed similar patterns and changes with development of pupal stage. In the first day of adult, MHP-c was clearly detected in both female and male of the Daizo(sdi), but not in the J037, indicating that there was significantly varietal differences in electrophoretical protein pattern. In addition, the results of protein pattern of hemolymph by SDS-PAGE showed also varietal differences in the concentration of hemolymph protein.

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Multi-beam Echo Sounder Operations for ROV Hemire - Exploration of Mariana Hydrothermal Vent Site and Post-Processing (심해무인잠수정 해미래를 이용한 다중빔 음향측심기의 운용 - 마리아나 열수해역 탐사 결과 및 후처리 -)

  • Park, Jin-Yeong;Shim, Hyungwon;Lee, Pan-Mook;Jun, Bong-Huan;Baek, Hyuk;Kim, Banghyun;Yoo, Seong-Yeol;Jeong, Woo-Young
    • Journal of Ocean Engineering and Technology
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    • v.31 no.1
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    • pp.69-79
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    • 2017
  • This paper presents the operations of a multi-beam echo sounder (MBES) installed on the deep-sea remotely operated vehicle (ROV) Hemire. Hemire explored hydrothermal vents in the Forecast volcano located near the Mariana Trench in March of in 2006. During these explorations, we acquired profiling points on the routes of the vehicle using the MBES. Information on the position, depth, and attitude of the ROV are essential to obtain higher accuracy for the profiling quality. However, the MBES installed on Hemire does not have its own position and depth sensors. Although it has attitude sensors for roll, pitch, and heading, the specifications of these sensors were not clear. Therefore, we had to merge the high-performance sensor data for the motion and position obtained from Hemire into the profiling data of the MBES. Then, we could properly convert the profiling points with respect to the Earth-fixed coordinates. This paper describes the integration of the MBES with Hemire, as well as the coordinate conversion between them. Bathymetric maps near the summit of the Forecast volcano were successfully collected through these processes. A comparison between the bathymetric maps from the MBES and those from the Onnuri Research Vessel, the mother ship of the ROV Hemire for these explorations, is also presented.

Numerical Analysis on Liquefaction Countermeasure of Seabed under Submerged Breakwater Using Concrete Mat Cover (for Irregular Waves) (콘크리트매트 피복을 이용한 잠제하 해저지반에서의 액상화 대책공법에 관한 수치해석 (불규칙파 조건))

  • Lee, Kwang-Ho;Ryu, Heung-Won;Kim, Dong-Wook;Kim, Do-Sam;Kim, Tae-Hyung
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.29 no.1
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    • pp.20-35
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    • 2017
  • In the case of the seabed around and under gravity structures such as submerged breakwater is exposed to a large wave action long period, the excess pore pressure will be significantly generated due to pore volume change associated with rearrangement soil grains. This effect will lead a seabed liquefaction around and under structures as a result of the decrease in the effective stress, and eventually the possibility of structure failure will be increased. The study of liquefaction potential for regular waves had already done, and this study considered for irregular waves with the same numerical analysis method used for regular waves. Under the condition of the irregular wave field, the time and spatial series of the deformation of submerged breakwater, the pore water pressure (oscillatory and residual components) and pore water pressure ratio in the seabed were estimated and their results were compared with those of the regular wave field to evaluate the liquefaction potential on the seabed quantitatively. Although present results are based on a limited number of numerical simulations, one of the study's most important findings is that a safer design can be obtained when analyzing case with a regular wave condition corresponding to a significant wave of the irregular wave.

A Case Study of GHG Reduction Based on Electricity Consumption Pattern of Individual Rooms : In case of Seoul National University (실별 전력 소비패턴에 의거한 온실가스 감축 잠재량 산정 - 서울대학교 관악 캠퍼스를 대상으로 -)

  • Kim, Seok-Young;Park, Moonseo;Lee, Hyun-Soo;Kim, Sooyoung;Jung, Hye-Jin
    • Korean Journal of Construction Engineering and Management
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    • v.14 no.4
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    • pp.55-64
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    • 2013
  • As GHG target management is introduced in Korea, designated establishment takes responsibilities to reduce more than 30% of expected GHG emission until 2020. Although decreasing GHG has been requested to universities which consume great amount of energy, there are difficulties to apply high cost countermeasures. Therefore, this research suggest a low cost, easily-applicable energy saving method, and derive potential GHG reduction amount in the case of SNU, Kwan-ak campus. First of all, 11 rooms of different use were chosen as the samples, and energy consumption in each room was measured. Standard models for each room were built through researching on the electric devices in each room. Moreover, energy consumption was computed for each devices through analyzing the pattern of electricity consumption. 32 GHG reduction technology and action program were chosen, and they were applied to the standard models for individual rooms. Through multiplying energy reduction rate of each program to energy consumption of each electric device, maximum energy reduction of each electric device is derived. Through that, Maximum GHG reduction for individual rooms and each month and the total GHG reduction capacity of Kwan-ak campus were computed. It was found out that approximately $5,311tCO_2$-eq can be reduced, when reduction technology and action program suggested by this research are applied. It appeared 24.48% of requested reduction amount to SNU can be reduced, till 2016.

Froth Flotation of Copper Ore from Jambi Deposit, Indonesia (인도네시아 잠비산 동광석의 부유선별 특성 연구)

  • Kim, Hak-Soon;Jeon, Ho-Seok;Kim, Byoung-Gon;Baek, Sang-Ho
    • Journal of the Mineralogical Society of Korea
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    • v.23 no.3
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    • pp.243-250
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    • 2010
  • Froth flotation of complex copper ore from Indonesia Jambi mine has been carried out to produce high-grade Cu concentrate. Since the ore contained minor Cu sulfides in addition to major Cu carbonate (malachite), copper concentrate was recovered by two-stage process of flotation, which consisted of copper sulfide flotation using xanthate followed by copper oxide flotation using oleic acid. The copper sulfide concentrate of 57.5% Cu grade with 9.5% recovery was obtained by copper sulfide flotation under conditions of 300 g/t collector (1 : 1 mixture of xanthate series Aero Promoter 211 and Aero Promoter 242) and pH 6.0 pulp. In subsequent copper oxide flotation on sink products, the concentrate of 30.8% Cu grade with 92.1% recovery was obtained under the conditions of oleic acid 300 g/t, AF65 50 g/t, pH 8.0 and 2 times cleaning. The flotation techniques which can achieve a Cu grade of 36.1% and a recovery of 92.1% have been developed from the two-stage process of flotation.

Effect of Korean Red Ginseng on Sleep : A Randomized, Placebo-Controlled Trial (고려 홍삼이 수면의 질에 미치는 영향 : 무작위 위약-대조군 연구)

  • Lee, Sun-Ah;Kang, Seung-Gul;Lee, Heon-Jeong;Jung, Ki-Young;Kim, Leen
    • Sleep Medicine and Psychophysiology
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    • v.17 no.2
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    • pp.85-90
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    • 2010
  • Objectives: Ginseng has a long history of being used in insomnia treatment and there is some evidence from animal studies of its sleep-enhancing property. From this, it can be assumed that ginseng has sleep-promoting effect in humans. The purpose of this study was to investigate the effect of Korean red ginseng on change of sleep architecture in humans. Methods: A total of 20 healthy young males with regular sleep and wake habits and without any psychiatric nor cognitive problems were selected based on review of sleep questionnaires and sleep diaries they completed followed by an interview with a board-certified psychiatrist. The subjects were randomly assigned to red ginseng or placebo for 2 weeks of trial. The total daily dose of ginseng was 4,500 mg. The polysomnographic recordings were made at baseline and at 2 weeks after. The effects of red ginseng and placebo on sleep were assessed by comparing the changes in polysomnographic variables between the two groups. Results: A total of 15 subjects, 8 from red ginseng group and 7 from placebo group, were included to undergo polysomnographic procedures. The red ginseng group showed tendencies to increase stage 3 sleep (p=0.087) and to decrease stage 2 sleep (p=0.071) from the baseline compared with the placebo group. Conclusion: Korean red ginseng tends to increase deep sleep and decrease shallow sleep. Our result is in line, at least in part, with previous findings that Korean red ginseng increased total and NREM sleep in rats. Further studies with higher ginseng dosage, larger sample size and longer trial duration should be conducted to confirm the sleep stabilizing and balancing effects of Korean red ginseng.

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Irregular Waves-Induced Seabed Dynamic Responses around Submerged Breakwater (불규칙파동장하 잠제 주변지반의 동적거동에 관한 수치해석)

  • Lee, Kwang-Ho;Ryu, Heung-Won;Kim, Dong-Wook;Kim, Do-Sam;Kim, Tae-Hyung
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.28 no.4
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    • pp.177-190
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    • 2016
  • In case of the seabed around and under gravity structures such as submerged breakwater is exposed to a large wave action long period, the excess pore pressure will be generated significantly due to pore volume change associated with rearrangement soil grains. This effect will lead a seabed liquefaction around and under structures as a result from decrease in the effective stress. Under the seabed liquefaction occurred and developed, the possibility of structure failure will be increased eventually. Lee et al.(2016) studied for regular waves, and this study considered for irregular waves with the same numerical analysis method used for regular waves. Under the condition of the irregular wave field, the time and spatial series of the deformation of submerged breakwater, the pore water pressure (oscillatory and residual components) and pore water pressure ratio in the seabed were estimated and their results were compared with those of the regular wave field to evaluate the liquefaction potential on the seabed quantitatively. Although present results are based on a limited number of numerical simulations, one of the study's most important findings is that a more safe design can be obtainable when analyzing case with a regular wave condition corresponding to a significant wave of irregular wave.

Variation Characteristics of Irregular Wave Fields around 3-Dimensional Low-Crested-Breakwater (3차원 저마루구조물(LCS) 주변에서 불규칙파동장의 변동특성)

  • Lee, Kwang-Ho;Lee, Jun Hyeong;Jung, Uk Jin;Kim, Do-Sam
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.32 no.2
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    • pp.122-134
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    • 2020
  • On the many coasts of South Korea, including the eastern side, it has been recently increasing the coastal disaster such as the severe coastal erosion and road damage swept away by the wave. As one of the alternatives to prevent the coastal disaster, it has been widely studied the coastal disaster reduction method by the Low-Crested Structure (LCS) in the many countries including several European countries. In this study, the olaFLow model is used to simulate the permeable LCS and wave field of the LCS through the three-dimensional irregular waves numerical analysis on the basis of the previous research. From the numerical analysis, it is evaluated the Hrms, nearshore current and time-averaged turbulent kinetic energy. In addition, the pattern of nearshore current and spatial distribution of time-averaged turbulent kinetic energy are compared with the case of submerged breakwater under the irregular wave fields. As one of significant results, it is confirmed that the pattern of nearshore current is different with the case of submerged breakwater.

Relatedness of Naturalized Bradyrhizobium japonicum Populations with Soil Physico-Chemical Characteristics as Affected by Paddy-Upland Rotation (답전윤환에 따른 토착 Bradyrhizobium japonicum의 서식밀도와 토양 이화학성과의 관계)

  • Park, Chang-Young;Youn, Moon-Tae;Choi, Sang-Uk;Ha, Ho-Sung;Kang, Ui-Gum
    • Applied Biological Chemistry
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    • v.40 no.5
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    • pp.438-441
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    • 1997
  • The relatedness of naturalized Bradyhizobium japonitum populations with soil physico-chemical characteristics as affected by paddy rice-upland soybean rotation cropping with conventional and none fertilization in Chilgog clay loam soils were determined as follows. The populations of B. japonicum in soils were increased from about $10^1$ in continuous paddy upto $10^1cells/g.soil$ only in one-year rotation of upland use with soybean cropping. Compared to the densities in plots of conventional fertilization, those in none fertilization were high ranging from 1.9 to 10 fold in 2-year upland use rotation and both in 3-year upland use rotation and 4-year upland use, respectively. The populations were positively correlated with soil organic matter $contents(r=0.83^*),\;Ca/K(r=0.74^*),\;and(Ca+Mg)/K(r=0.72^*)$ and were negatively correlated with soil $hardness(r=-0.73^*)$. And the soil populations increased by paddy-upland rotation resulted in superior symbiotic potentials to those in continuous paddy use in terms of nodule mass, nitrogenase activity, and soy-bean shoot dry weight.

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A Study on the Lipid Components of Silkworn Pupae (잠용의 지질성분(脂質成分)에 관(關)한 연구(硏究))

  • Kim, Jung-Ho;Park, Kwan-Hwa;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.26 no.2
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    • pp.90-96
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    • 1983
  • To study the composition of silkworm pupae oil, lipid of silkworm pupae was separated into two fractions, ether extractable and 85% methanol extractable, and the lipid components of each fraction were analyzed by using silicic acid column, thin layer chromatography and gas chromatography. Silkworm pupae contains 35.4% crude fat (dry-basis) of which consists 34.4% diethyl ether-extract and 0.9% of 85% methanolextract. The diethyl ether-extract contained 96.1% of neutral lipid, 2.9% of glycolipid and 1.0% of phospholipid while methanol-extract was consisted of 47.4% of neutral lipid, 14.6% of glycolipid and 38.1% of phospholipid. The major components of phospholipid were phosphatidyl glycol(41.0%), and phosphatidyl choline(28.2%) and phosphatidyl ethanolamine(21.2%) in the diethyl ether-extract and phosphatidyl glycol(48.4%), phosphatidyl inositol(22.8%) and phosphatidyl choline(17.9%) in the methanol-extract. The major fatty acids of the total lipid were oleic acid(33.5%), linolenic acid(31.0%) and palmitic acid(23.1%).

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