• Title/Summary/Keyword: 자외선 조사

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Effects of Pleurotus eryngii extract against inflammation in activated RAW 264.7 cells and UV-induced skin damage in mice (활성 RAW 264.7 세포에서 항염증 및 자외선 유도 마우스 피부손상의 개선에 대한 새송이 추출물의 효과)

  • Cho, Byoung Ok;Yin, Hong Hua;Lee, Hyun-Seo;Chu, Jung Im;Jang, Seon Il
    • Korean Journal of Food Science and Technology
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    • v.49 no.1
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    • pp.90-96
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    • 2017
  • The aim of this study was to investigate the anti-inflammatory and ultraviolet (UV)-protective effect of Pleurotus eryngii extract (PEE) in activated RAW 264.7 cells and UV-induced mouse skin damage. The results showed that PEE strongly inhibited the production of inflammatory mediators such as nitric oxide, interleukin $(IL)-1{\beta}$, and IL-6 at high concentrations in LPS-stimulated RAW 264.7 macrophages. In addition, PEE treatment suppressed erythema, melanin index, and epidermal thickness to a greater degree than ascorbic acid (AA) treatment in UV-irradiated mice. Finally, PEE treatment inhibited the infiltration of mast cell to a similar degree of AA treatment. Therefore, these results indicate that PEE could improve inflammation and skin damage in immune cells and UV-irradiated mice. This study may provide positive insights into PEE as a functional food and cosmetic ingredient for treatment of inflammation and skin damage.

Cell Fusion of Cellulolytic Fungi, Aspergillus sp. HB1 (섬유소 분해균 Aspergillus sp. HB 1의 세포융합에 관한 연구)

  • Kim, Joo-Ho;Chang, Sung-Yeoul;Choi, Yong-Keel
    • The Korean Journal of Mycology
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    • v.15 no.2
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    • pp.80-86
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    • 1987
  • The author isolated high cellulolytic fungi from natural sources and determined optimal condition of protoplast formation and fusion as fundamental step for improvement of the isolated it's cellulolytic ability. Three different cellulolytic fungi, such as Aspergillus sp., Penicillium sp. and Trichoderma sp., were isolated from soil. Their cellulolytic activities were compared with that of Aspergillus niger which was useful industrially and had cellulase activity. It was Aspergillus sp. that showed the highest activity of all these four fungi. And then it was followed by Penicillium sp., Trichoderma sp., and Aspergillus niger in order. An auxotrophic mutant of Aspergillus sp. was obtained by UV mutagenesis method. Having try to produce protoplast from mycelia, the author found that ${\beta}-glucuronidase$, at pH 6.0, was effective cell-wall lytic enzyme. And the optimal concentration of this enzyme was 5,000 unit/ml. Regeneration rates of wild type, met. auxotroph and arg. auxotroph, in presence of osmotic stabilizer, were 7. 0%, 7. 5% and 5.2%, respectively. PEG with M.W. 6,000 was effective stimulator for protoplast fusion in the concentration of 30% (W IV). In such a condition, we obtained 1.2% cell fusion rate.

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Synthesis of Ti-SBA-15 Doped with Lanthanide Ions and Their Photocatalytic Activity (란탄족 이온이 도핑된 Ti-SBA-15의 합성 및 그들의 광촉매 활성)

  • Hong, Seong-Soo
    • Clean Technology
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    • v.26 no.1
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    • pp.7-12
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    • 2020
  • Ti-SBA-15 catalysts doped with lanthanide ions (Ln/Ti-SBA-15) were successfully synthesized using conventional hydrothermal method. In addition, they were characterized by XRD, FT-IR, DRS, BET, and PL. The activity of these materials on the photocatalytic decomposition of methylene blue under ultraviolet light irradiation was also examined. Ti-SBA-15 catalysts doped with various lanthanide ions maintained their mesoporous structure. The pore size and pore volume of Ln/Ti-SBA-15 materials decreased but their surface area increased upon the doping of lanthanide ion. Ln/Ti-SBA-15 materials exhibited the type IV nitrogen isotherm with desorption hysteresis loop type H2, which was characteristic of mesoporous materials. The size of hysteresis increased in the doping of lanthanide ions on Ti-SBA-15 material. There was no absorption in the visible region (> 400 nm) regardless of the doping of lanthanide ions to TiO2 particles, while the broad bands at 220 nm appeared at the Ln/Ti-SBA-15 samples, indicating the framework incorporation of titanium into SBA-15. 1 mol% Pr/ Ti-SBA-15 catalysts showed the highest photocatalytic activity on the decomposition of methylene blue but the Ti-SBA-15 catalysts doped with Eu, Er, and Nd ions showed lower activity compared to pure Ti-SBA-15 catalyst. The PL peaks appeared at about 410 nm at all catalysts while the excitonic PL signal was proportional to the photocatalytic activity for the decomposition of methylene blue.

A Case Study for Deterioration due to Alkali-Silica Reaction in the Cement Concrete Pavement (알칼리-실리카 반응에 의한 시멘트 콘크리트 포장 파손 사례)

  • Hong, Seung-Ho;Han, Seung-Hwan;Yun, Kyong-Ku
    • Journal of the Korea Concrete Institute
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    • v.18 no.3 s.93
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    • pp.355-360
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    • 2006
  • The Alkali-Silica Reaction(ASR) may cause a serious failure in the concrete pavements and structures. Several researches in some nations have conducted the continuous studies to prevent failure of the concrete structures by the ASR distress as well as the studies to manifest the mechanism. The researches on the ASR have not been performed affluently in Korea because the distress due to ASR has seldom been reported literarily. In this study, we tried to set up the systematic scheme practically for verifying the cause of distress due to ASR by using the visual inspections in field, the chemical method, petrographic analysis, and Electron Dispersive X-ray Spectrometer(EDX) method of Scanning Electron Microscopy(SEM) in laboratory. The chemical method, petrographic method using SEM, and X-ray method were used to verify the cause of pattern crack on the surface and internal crack in the plain concrete pavement. It can be concluded that the distress of a specific site in plain concrete pavement was mainly due to ASR. The chemical method, the petrographic method and EDX method using SEM may be the effective tools for verifying the cause of AAR distresses.

Synthesis of PbMo1-xCrxO4 Oxides Prepared Using Hydrothermal Process and their Photocatalytic Activity (수열합성법에 의한 PbMo1-xCrxO4 산화물의 합성 및 광촉매 활성)

  • Song, Young In;Hong, Seong-Soo
    • Applied Chemistry for Engineering
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    • v.26 no.6
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    • pp.714-718
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    • 2015
  • Both lead molybdate ($PbMoO_4$) and chromium substituted lead molybdate ($PbMo_{1-x}Cr_xO_4$) were successfully synthesized using a conventional hydrothermal method and characterized by XRD, DRS, Raman, SEM and PL. We also investigated the photocatalytic activity of these materials for the decomposition of rhodamine B under UV-visible irradiation. The XRD and Raman results revealed the successful synthesis of well-crystallized $PbMoO_4$ crystals with the diameter of 51-59 nm, regardless of the addition of chromium ion. The DRS spectra of $PbMo_{1-x}Cr_xO_4$ catalysts showed new intensive absorption bands in the visible region. The $PbMoO_4$ catalysts showed the lowest photocatalytic activity and the activity increased with an increase of chromium substitution amounts under visible irradiation. PL peaks appeared at about 540-580 nm for all catalysts and excitonic PL signals were proportional to the photocatalytic activity for the decomposition of rhodamine B.

Ecological Studies on the Culture Bed and Production of Young Top Shell, Batillus cornutus in Cheju Island (제주도산 소라의 치패생산 및 서식생태에 관한 연구)

  • Pyen Choong Kyu;Youn Jeong Su
    • Journal of Aquaculture
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    • v.3 no.1
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    • pp.89-125
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    • 1990
  • In order to improve top shell seed production techniques spawning and larvae rearing were done in rearing tanks. Growth of young top shell in the nursing ground were also investigated. For induced spawning, top shells were maintained in still water during night time. Then they were treated with ultra violet iradiated sea water after dried up in air for 60 minutes. Spawning rate were 10 to $39.77\%$. It was found that young top shells moved in the growing grounds from nursing grounds when they reached approximately 30-40mm in shell heignt. Among main food algae for top shell in the natural growing grounds, sea mustard were melted away during June. Therefore, presence of another food algae such as Ecklonia cava or Sargassum spp. seems to be the main limiting factor for survival of top shell during summer. The tolerance of top shells ranging from 30mm to 60mm to low density of seawater for were tested at the temperature between 29.5 and $31.4^{\circ}C$. Hundred percent mortality occoured in 20, 55 and 90 hours after first stocking at the specific gravity of 1.010, 1.015, and 1.020, respectively.

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The Development of Filter for Environmental Improvement in Land Based Seawater Fish Farm II. Purification efficacy of Inlet and Outlet Seawater by Filter (필터의 개발을 통한 해수 육상수조식 양식장의 환경개선에 관한 연구 II. 필터 운행에 따른 유입수 및 배출수의 정화효과)

  • KANG Ju-Chan;PARK Soo-Il;KIM Seoung-Gun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.6
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    • pp.914-919
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    • 1998
  • This study was conducted to evaluate the purification efficacy by filter and ultra violet (UV) irradiation against inlet and outlet seawater of the land based fish farm. Purification efficacies for inlet seawater (50$\ell$/min) has been examined with filter of 60$\mu$m pore size and UV irradiation at an average dose 0.5 mWS/$cm^2$ for 5 months. For outlet seawater filter of 90 $\mu$m pore size only was used. Temperature, salinity, pH, $NO_3-N,\;NO_2-N,\;NH_4-N,\;PO_4-P$, DO and COD of inlet and outlet seawater in the system were not influenced. However, the removing rate of suspended solid and turbidity of inlet seawater were appeared to be $48.7\~65.6\%$ (average $51.9\%$) and $33/3\~42/5\%$ (average $34.8\%$) after passing through screen filter respectively. Also, germicidal efficiency to the total bacteris and Vibrio species were $16.7\~20.2\%$ (average $19.2\%$) $20.0\~21.9\%$ (average $20.9\%$) respectively after passing through UV irradiation. After passing through drum filter of 90$\mu$m pore size, suspended solid and turbidity of outlet seawater were appeared to be $42.7\~52.6\%$ (average $46.9\%$), $27.7\~29.9\%$ (average $28.3\%$), respectively.

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Free Radical Scavenging Activities and Inhibitory Effect on Xanthine Oxidase by Acetone Extract from Buckwheat (수원 5호 메밀 채소 추출물의 유리라디칼 소거 및 Xanthine Oxidase 활성 저해)

  • Kim, Young-Soon;Cho, Jung-Soon;Suh, Hyung-Joo
    • Applied Biological Chemistry
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    • v.40 no.3
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    • pp.254-258
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    • 1997
  • To examine the characteristics of the antioxidative property of Buckwheat components, acetone extracts from a buckwheat, Suwon 5, was fractionated using five solvents. Hexane, ethylacetate, ether, butanol and water fractions were obtained. Butanol fraction showed the greatest electron donating ability and inhibitory effect on lipid peroxidation. It also showed the most excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. Spectrophotogram of butanol fraction was similar to that of rutin. Superoxide radical scavenging activity was related to the contents of rutin. Inhibitory effect of each fraction on xanthine oxidase was also measured. Butanol fraction had the strongest inhibitory effect on xanthine oxidase and $IC_{50}\;was\;3.1\;{\mu}g$. The inhibition type of butanol fraction on xanthine oxidase turned out to be a mixture of the uncompetitive and non-competitive modes.

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Spatio-temporal Moth Diversity (Insecta: Lepidoptera) of Mt. Baegun-san, Gwangyang, Jeonnam (전라남도 광양시 백운산 나방 (곤충강: 나비목)의 시공간적 다양성 연구)

  • Choi, Sei-Woong;Nho, Dong-Ho;Kim, Sang-Soo;Hong, Kee-Jeong
    • Korean Journal of Ecology and Environment
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    • v.49 no.1
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    • pp.62-66
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    • 2016
  • This study was aimed to investigate the elevational and seasonal variation of moth diversity of Mt. Baegun-san, Gwangyang-city, Jeonnam. Moth collecting was carried out using a UV light trap at three different elevational sites from September to October 2014 and from April to October 2015 (9 times) except winter. A total of 288 moth species and 1,444 individuals in 15 families were identified. Geometridae (97 species and 740 individuals) were the most dominant in the numbers of species and individuals, followed by Noctuidae (62 species, 193 individuals), Erebidae (52 species, 177 individuals) and Notodontidae (16 species, 111 individuals). Elevational change showed that the lower elevation, the higher number of species and individuals. Seasonal change of numbers of species and individuals showed the M-shaped pattern with June and August peaks. Compared to the previous records from historical literatures, 197 species were newly added and the total moth species richness of Mt. Baegun-san comprised 449 species in 20 families. The Chao 2 estimated number of species using the observed species including unpublished data set was 1,530 species that comprised about 43% of the Korean Lepidopteran fauna. This large number of estimated species requested the more extensive and long-term survey for better understanding of the total moth fauna in this area.

Characteristics of ITO with surface treatment by N2, O2, Ar Plasma and UV (질소, 산소, 아르곤 플라즈마와 자외선에 의하여 표면 처리한 ITO의 특성)

  • Bae, Gyeong-Tae;Jeong, Seon-Yeong;Gang, Seong-Ho;Kim, Hyeon-Gi;Kim, Byeong-Jin;Ju, Seong-Hu
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.90-90
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    • 2018
  • 디스플레이는 다수의 가로 전극과 세로 전극으로 구성되고, 전극에 신호를 주어 동작하도록 하는 원리이다. 이 디스플레이에는 전기가 통하고 투명한 전극이 필수적으로 사용되고 있고, 대표적인 투명 전극으로 ITO (Indium Tin Oxide)가 있다. ITO 박막은 $In_2O_3$에 Sn을 첨가하여 $Sn^{4+}$ 이온이 $In^{3+}$ 이온을 치환하고 이 과정에서 잉여 전자가 전기전도에 기여하는 구조이다. ITO 박막은 표면 처리 방법에 따라 표면 상태가 크게 변화한다. 플라즈마를 이용한 표면 처리는 환경오염이 적으며 강도, 탄성률 등과 같은 재료의 기계적 특성을 변화시키지 않으면서 표면 특성만을 변화시킬 수 있는 방법으로 알려져 있다[1]. UV (Ultraviolet)를 조사한 표면처리는 ITO 표면의 탄소를 제거하고, 표면 쌍극자를 형성하며, 표면의 조성을 변화시킬 수 있으며, 페르미 에너지 준위를 이동시킬 수 있어 ITO의 일함수를 증가시킬 수 있다[2]. ITO에 대한 다양한 연구가 수행되었음에도 불구하고 보다 다양한 관점에서의 연구가 지속될 필요가 있다. 따라서 본 연구에서는 다양한 조건으로 표면 처리한 ITO 표면의 일함수, 면저항, 표면 형상, 평탄도, 접촉각 등에 대해 알아보고자 한다. 세정한 ITO, 세정 후 UV 처리한 ITO (UV 처리 시간 2분, 4분 6분, 8분), 세정 후 $N_2$, $O_2$, Ar의 공정 가스를 사용하여 Plasma 처리한 ITO로 표면 처리 조건을 변화하였다. 표면 처리한 ITO의 특성은 Kelvin Probe를 이용한 일함수, 물방울 형상의 각도를 측정한 접촉각, AFM (Atomic Force Microscope)을 이용한 평탄도, 가시광선 (380~780 nm) 파장에 대한 투과도와 면저항을 측정하였다. 접촉각은 세정한 ITO의 경우 $45.5^{\circ}$에서 세정 후 UV를 조사한 ITO의 경우 UV 8분 조사 시 $27.86^{\circ}$로 감소하였고, $N_2$, $O_2$, Ar 가스를 사용하여 Plasma 처리한 ITO는 모두 $10^{\circ}$ 미만을 나타내었다. 플라즈마 처리에 의하여 접촉각이 현저하게 개선되었다. ITO의 면저항은 표면 처리 조건에 따라 $9.620{\sim}9.903{\Omega}/{\square}$로 그 차이가 매우 적어 표면처리에 의하여 면저항의 변화는 없는 것으로 판단된다. 가시광선 영역에서의 투과도는 공정 조건에 따라 87.59 ~ 89.39%로 그 차이가 적어 표면처리에 의한 변화를 나타내지는 않은 것으로 판단된다. 표면 처리 조건에 따른 평탄도 $R_{rms}$는 세정한 ITO의 경우 4.501 nm로부터 UV 2, 4, 6, 8분 처리한 경우 2.797, 2.659, 2.538, 2.584 nm로 평탄도가 개선되었다. $N_2$, $O_2$, Ar 가스를 사용하여 플라즈마 처리한 ITO의 경우 평탄도 $R_{rms}$는 2.49, 4.715, 4.176 nm로 사용한 가스의 종류에 따라 다른 경향을 나타내었다. 표면 처리 조건에 따른 평탄도 Ra는 세정한 ITO의 경우 3.521 nm로부터 UV 2, 4, 6, 8분 처리한 경우 1.858, 1.967, 1.896, 1.942 nm를, $N_2$, $O_2$, Ar 가스를 사용하여 플라즈마 처리한 ITO의 경우는 1.744, 3.206, 3.251 nm로 평탄도 $R_{rms}$와 유사한 경향을 나타내었다.

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