• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.87-94
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• 2002

This study was performed during the four seasons for the production of transgenic pigs containing the Cellulase Digest Gene. Purebred Landrace gilts and sows approximately 8∼15 months of age (n=126) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality fur zygote collection. Synchronization and superovulation procedures were used that cyclic gilts were synchronized with 20mg altrenogest (ALT) per day for 9 days after PG600 administration followed by superovulation with 1000 IU pregnant mares serum gonadotropin (PMSG) and 750IU human chorionic gonadotrophin (hCG). The cellulase digestion gene for microinjection is rat elasterase promoter (rEl) linked to CelD gene. After hormone treatment, 1,422 embryos were collected from 91 donors and 95.6% (1,359/1,422) embryos were in 1-cell stage which can be visualized the pronuclei for DNA microinjection. A total of 725 DNA microinjected embryos transferred into 35 recipients and produced 65 piglets from 13 litters. Pregnancy rate according to the number of transferred embryos to recipients was higher the group which received 21 to 24 embryos (50.0%) than other groups 20.0% in less and 33.3% in more. A tail tissue was collected from 65 piglets for biopsy. PCR screening was performed on each DNA sample using two separate sets of primers specific for the 5'- and 3'-flanking region of the rEl-CelD gene. Five of the 65 piglets (7.69%) were positive for the transgene. This study provide useful information regarding production of transgenic pig for bioreactor research.

• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.917-924
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• 1995

I suis cocciosis in piglets seems to occur in the majority of Korean sow herds. Cocci야osis is characterized by a pasty diarrhea in piglets appearing at 5 to 11 days old. Morbidity is variable, Mortality is usually low but piglets growth is always retarded. And, the principal source of infection is the environment because of the high resistance of oocysts. Control of coccidiosis is usually frustrated since the majority of treatment have not constant results. Mundt et al(1990) developed a new strategy by administrating toltrazuril as an oral suspension. This new anticoccidial drug reduced clinical signs and mortality. Therefore, we will try to medicate piglets with Baycox(Bay Vi 9142) suspension in a sow herd, having a problem of I suis coccidiosis. The incidences of coccidiosis in suckling piglets in C and D bleeding stock farm were from 28.6 to 42.3 and 21.1~57.9 % during 3 to 21 day-old. The body weights of suckling piglets in treated groups were higher than those of control groups at 11 and 18 day-old. Also, those of C breeding stock firm were higher than those of D farm. The body weight gains of suckling piglet of treated groups were higher than those of control groups. Also, those of C breeding stock firm were higher than those of D farm. There was no dead piglet in all experimental groups. Some atrophic piglets were done away with. In C breeding stock farm, the incidences of diarrhea in treatment groups were significantly lower an those of control groups. Also, the degrees of diarrhea in treated groups were significantly lower than those in control groups. In D breeding stock farm, the incidences of diarrhea in treated groups were a little lower than those in control groups. Also the degrees of diarrhea in treated groups were a little lower than those in control groups. In the treated groups, the incidences and degrees of diarrhea were reduced at the 4th day and increased at the 13th day after treatment. The number of litters excreted oocysts in treated groups wert lower than those in control groups after treatment. Also, the OPG in treated groups were lower than those in control groups. According to above results, the effect of Baycox suspension against swine coccidiosis was very good. If we will treat with Barcox suspension swine coccidiosis in swine stock farms, we can get good effects of the improvement of body weight gains and diarrhea and e reduce of excreted costs in faeces.

• Korean Journal of Organic Agriculture
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• v.26 no.1
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• pp.141-149
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• 2018

This experiment was conducted to investigate the effects of multi-enzyme on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multi-enzyme (Multi; mixture of ${\beta}-mannanase$, xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Measurements were growth performance, morphology of ileum, apparent ileal digestibility and apparent total tract digestibility of dry matter, crude protein, and energy of weaned pigs. There were no significant differences on growth performance during overall experimental period. No differences were found for the morphology of ileum and nutrient digestibility between CON and Multi groups. Therefore, the results in the current study indicated that multi-enzyme supplementation in diets had no effects on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

• Korean Journal of Organic Agriculture
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• v.26 no.1
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• pp.151-161
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• 2018

This study was designed to investigate the effects of multi-enzyme on diarrhea and immune responses of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multienzyme (Multi; mixture of ${\beta}-mannanase$, xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Frequency of diarrhea, levels of packed cell volume (PCV), white blood cells (WBC), immunoglobulins, cortisol, tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), transforming growth $factor-{\beta}$ ($TGF-{\beta}$), and C-reactive protein (CRP) were measured. Multi group tended to decrease (p<0.1) diarrhea frequency than CON group during 2 wk after weaning. Lower values of PCV on d 3 (p<0.05) and d 7 (p<0.1) were found in Multi group compared with CON group. There were no significant differences on WBC number and immunoglobulin (Ig) M and A between Multi and CON groups. However, Multi group tended to increase (p<0.1) Ig G on d 7 than CON group. Moreover, Multi group showed modulated immune responses, indicated by decreased levels of cortisol (p<0.05) on d 7 and 14, $TNF-{\alpha}$ on d 3 (p<0.05) and d 7 (p<0.10), $TGF-{\beta}$ on d 2 (p<0.05) and d 7 (p<0.10), and CRP (p<0.10) on d 3 and 7 after weaning compared with CON group. Consequently, inclusion of multi-enzyme in diets for weaned pigs improved gut health and modulated immune responses of weaned pigs.

• Journal of Animal Environmental Science
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• v.15 no.2
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• pp.131-138
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• 2009

This study was conducted to find ways to control environment with the difference between body temperature and background temperature based on swine activity, and to apply to the environment control system of swine barns based on the findings. Following are the results. 1. Swine activity related to background temperature was achieved as color images and swine activity status was categorized into cold, comfortable, and hot periods with visualization system (thermal image system). 2. Thermal image system consisted of an infrared CCD camera, an image processing board - DIF (TH3100), an main computer (400Hz, 128M, 586 Pentium model) with C++ program installed. 3. Thermal image system categorizing temperatures into cold, comfortable, and hot was applicable to the environment control system of swine barns 4. Feed intake was higher in cold temperature, and finishing weight and weight gain per day in cold temperature were lower than others (p<0.05).

• 사료
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• s.42
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• pp.20-25
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• 2010

2009년은 그야말로 새옹지마(塞翁之馬 : 인생에 있어서 길흉화복은 항상 바뀌어 미리 헤아릴 수 없다)와 같은 한 해로 기억에 오래 남을 듯 하다. 2008년 말에 2009년 양돈산업을 전망할 때 미국발 금융위기에도 불구하고 국내산 돈육 공급량 부족과 환율상승 및 급변으로 인한 돈육 수입량 부족, 그리고 수급 불균형으로 대부분의 전문가들이 역대 최고의 돈가를 예상했고, 실제로 3월에 지육가격이 5,000원/지육kg(전국 비육돈 평균시세)을 상회하면서 성수기에 돈가가 6,000원/지육 kg에 육박할 것이라 기대했었다. 그러나 4월 미국에서 발생한 신종인플루엔자가 초기에 돼지에서 유래된 인플루엔자라고 보도되면서 돈육소비량이 급감하였고 이로 인해 성수기에 돈가가 오히려 하락하는 기현상을 보였다. 다행히 신종플루가 돼지와 무관하다는 사실이 밝혀지면서 소비가 회복되었고 돈가 또한 회복되어 올해는 역대 최고의 돈가가 형성될 것으로 예상되고, 여기에 국제곡물시세가 안정되면서 사료가격도 여러 차례 인하되어 양돈 농장의 수익성은 상당부분 향상될 것으로 판단된다. 하지만 신종플루 사망자가 증가하면서 지금도 여전히 돈육소비가 위축되어 있어 막연히 호황을 기대하기 쉽지 않은 실정이다. 돈가의 급등과 급락, 외부요인에 의한 경제환경의 변화로 인한 환율의 변동 및 소비량의 변화는 2009년 양돈산업에 대한 불안감을 증폭시켜 높은 돈가와 수익성에도 불구하고 전체 산업의 규모가 커지지는 못했다. 여기에 국산 돈육에 대한 소비자들의 무한 신뢰로 인해, 2008년 12월 돈육 원산지 표시제 확대 실시로 국내산 돈육 수요가 증가 하여 여전히 75% 가량의 국산 돈육 자급율을 유지함에도 불구하고 아직까지 맛의 차별화 및 도축, 유통의 안전성에 대한 확고한 신뢰 구축을 위한 브랜드 돈육 유통의 확대가 소비자의 기대치만큼 자리 잡지 못하고 있는 실정이다. 또한 친환경적인 양돈산업 육성 및 분뇨 처리 관련하여도 뚜렷한 해답을 찾지 못하고 있어, 양돈 농가의 입장에서는 규모의 확장 및 신규 진입이 극도로 제한되어 있고 일부 지역에서는 사업의 존폐를 결정하는 주요 원인이 되고 있다. 그간 대한민국 양돈 산업의 경쟁력에 발목을 잡고 있던 낮은 생산성 문제와 소모성 질병에 의한 높은 폐사율 문제 또한 아직도 여전히 해결되지 못한 과제이다. <그림 1> 국가별 양돈 농장 생산성 현황에서 보는 바 와 우리나라 양돈 농장의 생산성은 여전히 PSY(모돈당 연간 이유자돈 두수)가 18두에 못 미치고 있어 국제 경쟁력을 논하기 자제 가 부끄러운 실정이다. 여러 가지 내 외부적인 어려운 환경에도 불구하고 올 2009년은 양돈농가들이 최고의 수익성을 기록한 한 해가 될 것이라는 사실에는 이의가 별로 없을 듯하다. 2년 여간의 고돈가로 인해 대한민국 양돈산업은 새로운 도약을 위한 밑천이 마련된 셈이라고 할 수 있다. 본고에서는 2009년 양돈산업 현황을 돌아보고 다가올 미래를 준비한다는 의미에서 우리나라 양돈산업이 집중해야 할 분야를 짚어보고자 한다.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.16-27
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• 2019

Using virtual reality technology, users can learn and experience many interactions in virtual space like the actual physical space. This study was conducted to develop air flow simulator that allows farmers and consultants to consult air flow through VR devices by creating a greenhouse or pigpen model. It can help educate farmers about the importance of ventilation effects for agricultural facilities. We proposed CFD visualization system by building a virtual reality environment and constructing database of CFD and structure of agricultural facilities. After consultants can set up situations according to environmental conditions, the users experience the visualized air flow of agricultural facility according to the ventilation effects. Also it can provide a quantified environmental distribution in the agricultural facility. Currently, the CFD data in agricultural facilities are established during winter and summer. In order to experience various environmental conditions in the developed system, The experts need to run CFD data under various environmental conditions and register them in the system requirements.

• Korean Journal of Veterinary Service
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• v.42 no.1
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• pp.1-7
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• 2019

Porcine reproductive and respiratory syndrome (PRRS) is economically the most important and challenging disease in swine industries worldwide and caused by PRRS virus (PRRSV). Previous studies reported that pigs with heterozygous genotypes in the guanylate-binding proteins (GBP1 and GBP5) exhibited increased resistance against PRRSV infection. The present study was conducted to produce higher numbers of the heterozygous pigs based on the PRRS resistant polymorphisms found in GBP1 (GBP1E2 and WUR) and GBP5, and evaluate the resistance of heterozygous pigs against challenge with a type 2 PRRSV (JA142) in comparison with homozygous pigs. In the challenge study, 12, 4 week-old PRRSV-negative piglets were selected based on the genotypes of the 3 polymorphisms (GBP1E2, WUR and GBP5). Among them, 8 piglets [homozygous (n=4) and heterozygous (n=4)] were challenged with JA142 and kept in the same room, and the remaining 4 piglets were kept separately as a negative control. In results, the sperms collected from the boars of GBP1E2-GG genotype produced approximately 28~41% higher numbers of heterozygous piglets as compared with those from the boars of GBP1E2-AG genotype. In the challenge study, we found that heterozygous piglets showed the significantly lower levels of viremia than homozygous piglets at 14, 21 and 28 dpc. Consistently, these heterozygous piglets also exhibited significantly higher ADWG than homozygous piglets. Therefore, in the current study, selection of boars based on SNP markers could increase the production of PRRS resistant piglets and the PRRS resistant pigs were found to be more resistant to PRRSV infection.

• Journal of the Korea Organic Resources Recycling Association
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• v.25 no.3
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• pp.91-98
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• 2017

The purpose of this study is to provide a design and operation technical guideline for meeting the appropriate design criteria to bio-gasification facilities treating organic wastes. 9 anaerobic digestion facilities which is normally operated during the field survey and 14 livestock manure farms were selected for precision investigation. the physicochemical analysis was performed on the moisture and organic contents, nutrients composition (carbohydrate, fat, protein), volatile fatty acids (VFAs), and nitrogen, etc. Volatile solids (VS) of organic wastes brought into the bio-gasification facilities were 2.81 % (animal manure only) and 5.92 % (animal manure+food waste), respectively. Total solids (TS) reults of samples from livestock farms were 5.6 % in piglets and 11~13 % in other kinds of breeding pigs. The actual methane yield based on nutrients contents was estimated to $0.36Sm^3CH_4/kgVS$ which is equivalent to 72 % of theoretical methane yield value. The optimum mixing ratio depending on the effect of the combined bio-gasification was obtained through the continuous stirred-tank reactor (CSTR) which is operated at different mixing ratio of swine manure and food waste leachate. The range of swine manure and food waste leachate from 60:40 to 40:60 were adequate to the appropriate conditions of anaerobic digestion; less than 100 gTS/, more than alkalinity of 1 gCaCO3/L, C/N ratio 12.0~30.0, etc.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.155-163
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• 2004

The aims of this study are 1) to test oocytes and embryos collected from in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to Funahashi et al (1994). Glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at $39^{\circ}C$, and 10% fetal bovine serum albumin was added to the culture medium thereafter. Embryos were treated with 7.5 ${\mu}g/ml$ cytochalasin-B for 30 min, centrifuged at 13,000 rpm for 13 min and then exposed sequentially to an ethylene glycol(EG) vitrification solution, aspirated into OPS, and plunged/thawed into/from liquid nitrogen. In vivo embryos were surgically collected from three dornors after AI for control group. Forty-nine embryos were washed 3 times in mPBS + 10% FBS, followed treatments : cultured, centrifuged, vitrified, recovered and transferred to recipients as in vitro prepared embryos. Three recipients were transferred individually with 100, 100 frozen embryos derived from abattoir and 34 fresh embryos by surgically, and another three recipients were transferred individually with 150, 150 frozen embryos and 100 fresh embryos by nonsurgically, respectively. all recipient sows exhibited delayed returns to estrus. To our knowledge, theses results suggest that required an improved techniques, more vigorous embryos preparation and substitute to gilt with cleaner uterous condition.