• Journal of Acupuncture Research
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• v.24 no.4
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• pp.107-113
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• 2007

목적 : 본 연구는 계혈등 약침액이 자궁경부암 세포주 SNU-17에서 세포 사멸 효과가 있는지 알아보고자 하였다. 방법 : 자궁경부암 세포주 SNU-17에서 세포 사멸의 변화를 관찰하기 위해서 MTT cytotoxicity assay, DAPI staining, TUNEL assay, RT-PCR analysis 방법을 이용하였다. 결과 : 세포독성 검사에서 계혈등 약침액은 자궁경부암 세포주 SNU-17에 농도 의존적으로 세포독성을 나타내었다. 이러한 계혈등 약침액의 세포독성이 세포사별로 인한 것인지 다른 기전에 의한 것인지 알아본 결과 계혈등 약침액에 의한 세포독성은 DAPI staining 과 TUNEL assay에서 세포사멸의 특정적인 소견들을 나타내었다. 계혈등 약침액이 Bax, Caspase-3의 발현에 미치는 영향을 RT-PCR로 관찰한 결과 계혈등 약침액은 Bax, Caspase-3의 발현을 증가시켰다. 결론 : 이상의 결과 계혈등 약침액이 자궁경부암 세포주 SNU-17에서 세포 사멸을 야기하여 자궁경부암의 치료에 유용할 것으로 사료된다.

• Biomedical Science Letters
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• v.2 no.2
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• pp.153-158
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• 1996

Verapamil, a potent calcium channel blocker, has been proved to be one of the modulators to overcome drug resistance in cancer chemotherapy. In the present experiment, the possibility of verapamil as a MDR modulator was investigated by using MTT assay. Sole treatment of verapamil on the HeLa and Caski cervical cancer cell line revealed dose dependent cytotoxicity within a range of tested dose. Combined treatment of verapamil with 5-FU, DDP on two human cervical cancer cell line led to a significant synergistic cytotoxicity. Therefore , these studies showed that verapamil had a possibility to be applicable to cancer chemotherapy in gynecological oncology.

• The Science & Technology
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• v.29 no.11 s.330
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• pp.80-81
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• 1996

우리나라 여성들에게서 발병 사망률이 높은 자궁경부암의 세포주를 배양하여 암의 발생원인 특성을 밝혀낸 가톨릭대 의대 김진우교수. 김교수는 난소암 자궁내막암 세포배양에도 성공하여 앞으로 임상실험을 하기 위한 재료확보와 암의 항원 전체구조를 파악하는 연구를 계속할 것이라고 한다.

• 한국약용작물학회:학술대회논문집
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• 2008.05a
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• pp.188-189
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• 2008

• Proceedings of the Korean Information Science Society Conference
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• 2000.04b
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• pp.511-513
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• 2000

PaP Smear 테스트는 자궁 경부암 진단에 가장 효율적인 방법으로 알려져 있다. 그러나 이 방법은 높은 위 음성률(false negative error, 15~50%)을 나타내고 있다. 이런 큰 오류율은 주로 다량의 세포 검사에 기인하여, 자동화 시스템의 개발이 절실히 요구되고 있다. 본 논문은 자궁 경부암의 특징인 군집을 이루는 암세포를 인식할 수 있는 시스템을 제안한다. 시스템은 두 부분으로 나누어진다. 첫 단계에서는 저 배율(100배)에서 간단한 영상처리와 최소 근접 트리(Minimum Spanning Tree)를 통해 군집을 이루는 세포를 찾는다. 두 번째 단계서는 고 배율(400배)로 확대하여 군집 세포들로부터 여러 가지 특징을 추출한 후 KNN(k-Neighbor) 방법을 통해 인식하는 단계이다. 50개의 영상 (640X 480, RGB True Color 25 개의 100배 영상 , 25개의 400배 영상)이 실험에 사용되었다. 한 영상을 처리하는데 약 3초 (2.984초) 소요되었으며, 이는 region growing(20초)나 split and merge(58초) 방법 보다 덜 소요되었다. 100배 영상에서 정상과 비정상의 두 그룹으로 나누었을 경우에는 96%의 높은 인식율을 나타내었으나 비정상을 다시 5개의 그룹으로 나누었을 때는 45%로 나타내었다. 이는 영역 추출(segmentation) 단계에서 오류와 트레이닝 데이터의 비정확성에 기인한다. 400배 영상에서는 각각 92%와 30%로 나타내었다. 이는 영역추출 단계에서 사용한 Watershed 방법의 오류로 기인한 것으로 본다.

• Radiation Oncology Journal
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• v.18 no.4
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• pp.300-308
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• 2000

Purpose :The aim of this study is to analysis of suwival and recurrence rates of the uterine cervical carcinoma patients whom received the radiation therapy respectively. The prognostic factors, such as Papanicolaou (Pap) smear, carcinoembriogenic antigen (CEA) and squamous cell carcinoma (SCC) antigen has been studied. Methods and Materials : From January 1981 to December 1998, eight-hundred twenty-seven uterine carvical cancer patients were treat with radiation therapy. All of the patients were divided into two groups : the radiation therapy only (S2l patients) group and the postoperative radiation therapy (326 patients) group. The age, treatment modality, clinical stage, histopathology, recurrence, follow-up Pap smears, CEA and SCC antigen were used as parameters for the evaluation. The prognostic factors such as survival and recurrence rates were peformed with the Kaplan-Meier method and the Cox hazard model, respectively. Median rollow-up was 38.6 months. Results :On the radiation therapy only group, 314 patients (60$\%$) achieved complete response (CR), 47 patients (9$\%$) showed local recurrence (LR), 78 patients (15$\%$) developed distant metastasis (DM). On the Postoperative radiation therapy group, showed 276 Patients (85$\%$) CR, 8 Patients (2$\%$) LR, 37 Patients (11$\%$) DM. The 5-year survival and recurrence rates was evaluated for all parameters. The statistically significant factors for the survival rate in univariate analysis were clinical stage (p=0.0001), treatment modality (p=0.0010), recurrence (p=0.0001), Pap smear (p=0.0329), CEA (p=0.0001) and SCC antigen (p=0.0001). Conclusion: This study indicated that after treatment, the follow-up studies of Pap smear, CEA and SCC antigen were significant parameter and prediction factors for the survival and recurrence of the uterine cervical carcinoma.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.10
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• pp.1525-1530
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• 2005

The purpose of this study is to investigate anti-tumor activities, general composition, elemental composition and mineral contents of fermented liquid stem, root and fruit of Opuntia humifusa. In the general composition, the energy, crude protein, crude lipid and crude carbohydrate contents of fermented liquid stem were 86.21 Kcal, 0.92$\%$, 0.12$\%$, and 20.34$\%$, respectively. Fermented liquid fruit showed 65.32 Kcal, 1.04$\%$, 0.08$\%$, and 15.15$\%$. In mineral analysis, fermented liquid stem and fruit showed 1,800 and 388 mg of calcium per 100g. The ferrous concentrations of fermented liquid stem and fruit were 21 and 10 mg per 100 g, respectively. Methanol, ethanol and water extracts of nonfermented liquid stem and fruit did not inhibit the proliferation in human cervical cancer cells (Caski, SiHa and HaCaT), but the fermented liquid fruit showed the inhibition of Proliferation with dose-response manner in Caski and SiHa cells, but not HaCaT. Therefore, it suggests that fermented cactus may be used as one of potential adjuvant for the treatment of cervical carcinomas.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1389-1395
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• 2000

Cervical cancer has been one of the leading causes of female death from cancer worldwide with about 500,000 deaths per year. A strong association between certain human papillomaviruses (HPV types 16 and 18) and cervical cancer has been well known. An extract of natural products, Rhus, has been used to investigate whether this agent has the ability of inhibiting the oncogenes E6 and E7 of HPV type 16. This Rhus inhibited the proliferation of human cervical cancer cell lines (C-33A, SiHa, Caski) and HaCaT keratinocytes in a dose response manner. In vitro binding assay and ELISA showed that Rhus inhibited the in vitro binding of E6 and E6AP which are essential for the binding and degradation of the tumor suppressor p53. In addition, Rhus inhibited the in vitro binding of E7 and Rb which essential tumor suppressor for the control of cell cycle. The level of mRNA for E6 was also decreased by Rhus while that of E7 mRNA was not changed. Our data suggested that Rhus inhibited the oncogenecity of E6 and E7 of HPV 16 type, thus can be used as a putative anti-HPV agent for the treatment of cervical carcinomas by HPV.

• Radiation Oncology Journal
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• v.24 no.1
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• pp.37-43
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• 2006

Purpose: We wanted to determine the clinical characteristics and prognosis according to the VEGF expression in stage II cervical carcinoma patients treated with definitive radiotherapy. Materials and Methods: We enrolled 31 patients who were diagnosed with cervical cancer from 1995 to 2003 at Samsung Medical Center and their paraffin block tissue samples were available for study. The median age of the patients was 65 years. The mean tumor size was 4.1 cm $(range:\;1.2{\sim}8.2cm)$. Seven patients (22.6%) were suspected of having pelvic lymph node metastasis. An external beam irradiation dose of 45-56.4 Gy was administered to the whole pelvis with a 15 MV linear accelerator, and an additional 24 Gy was given to point A by HDR intracavitary brachytherapy. VEGF staining was defined as positive when more than 10% of the tumor cells were stained. The median follow-up duration was 58 months. Results: A positive VEGF expression was observed in 21 patients (67.7%), There was no significant correlation between the VEGF expression and pelvic lymph node metastasis, tumor size and the response of radiotherapy. During follow-up, 7 patients had recurrence. The complete response rate was not significant between the VEGF(-) and VEGF(+) tumors. However, the VEGF(+) tumors showed a significantly higher recurrence rate in comparison with the VEGF(-) tumors (p=0.040), The three year disease-free survival rates were 100% and 66.7%, respectively, for patients with VEGF(-) or VEGF(+) tumor (p=0.047), Conclusion: The VEGF expression was a significant factor for recurrence and disease-free survival. However, the significance of the VEGF expression is still controversial because of the various definitions of VEGF expression and the mismatches of the clinical data in the previous studies.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04c
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• pp.401-403
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• 2003

인유두종 바이러스는 표피세포에 감염되는 DNA 바이러스로 자궁경부암을 일으키는 가장 큰 요인이다. 현재까지 100 여개의 종류가 알려져 있고 악성종양 유발 가능성에 따라 위험군을 나누는데. 여기서 중요한 것은 고위험군을 저위험군으로 잘못 분류하는 것을 최소화하는 것이다. 본 논문에서는 분류를 위한 데이터로 인유두종 바이러스에 관한 문서 자료들을, 기계 학습 방법으로 분류 비용을 고려해 줄 수 있는 비용 의존 학습을 이용하였다. 실험결과. 비용을 고려해 주는 것이 고려하지 않았을 때보다 더 종은 성능을 나타내었다.