• Korean Journal of Environmental Agriculture
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• v.26 no.4
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• pp.319-324
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• 2007

This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.26-34
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• 2015

This study was conducted to examine the morphological characteristics and variations of the 6 populations of Pourthiaea. villosa (Thunb.) Decne. var. villosa in Korea. The ANOVA results showed that the populations were significantly different in 18 of the 21 quantitative characteristics that were analyzed. In the results of principal component analysis, 6 principal components (PC) represented 68.28% for the total variations. 'Petiole length' (0.764), 'Leaf shape' (0.834), 'Leaf length' (0.753), 'Crown diameter' (0.663), 'Inflorescence width' (0.492), and 'Leaf base shape' (0.721) showed the highest contribution to PC1, PC2, PC3, PC4, PC5, and PC6, respectively. According to the results of cluster analysis, one cluster comprised only 'Goesan-gun Ssanggok Valley' population. 'Namhae-gun Mijori Evergreen Forest' population was grouped with 'Hamyang-gun Sang Forest' population, which was the nearest subgroup. Further, 'Namhae-gun Geum Mountain' population, 'Jindo-gun Cheomchal Mountain' population, and 'Jeju-si Barimae Parasitic volcano' population were placed in the same cluster.

• Korean Journal of Plant Tissue Culture
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• v.24 no.3
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• pp.153-160
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• 1997

Cucumber mosaic virus (CMV) leads to a cause of poor crop productivity and quality. To solve this problem, we attempted to develop a virus-resistance tobacco plants by using viral coat protein (CP) gene. Transgenic tobacco plants expressing CMV CP gene were analysed by the resistance upon CMV infection. The virus-resistance was measured in $\textrm{T}_{1}$, generation by the inhibition of plant growth and the expression of the mosaic symptoms infected with CMV. The transgenic lines were divided into four groups: highly resistant, resistant, moderate and susceptible based on their growth and symptom severity. Out of 39 transgenic lines, 16 lines showed significant virus-resistance. And of resistant lines, 2 lines were designated highly resistant based on the facts that they achieved similar plant height to that of non-infected tobacco plants and showed lower disease symptom than that of other lines. The steady state level of CP RNA and coat protein level were measured by northern blot and immunoblot analysis. The CP RNA was highly accumulated in most resistant and moderate lines but barely detected in susceptible lines. The coat protein was detected in most lines regardless of their resistance to CMV. from this result, virus-resistance appeared to correlate more with CP RNA level than the level of coat protein. However, in two highly resistant lines, CP RNA level was unexpectedly low. This unexpected phenomenon need to be further investigated.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.409-413
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• 2015

To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both $TgMTP_1$ (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that $TgMTP_1$-overexpressing plants may be useful for phytoremediation of soil.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.36-42
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• 2002

Sixteen Atractylodes japonica Koidz. accessions were collected in Jeonbuk, Kyeongnam, Kangwon and Kyeonggi province, and their quantitative characteristics were compared with each other. The relationship of variation between Atractylodes japonica Koidz. accessions was also investigated by means of multivariate analysis. Hamyang 1 accession had the most desirable quantitative characteristics among the accessions: plant height was 86.2cm, number of leaves, 117.2 and number of stems, 34.3. Sudo 1 accession had 12 stems, the most among the accessions. The length of leaf margin serras found in all of them was on average 1.6mm. Most accessions had simple or palmately compound leaf with oval or elliptical type of the leaf shape. The accessions could be classified into three groups by average linkage cluster method. The most remote relationship was found between Group 1 containing Jinan 1, Jinan 2 and Group 3 containing Jeongsu, and Geoje.

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.46-50
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• 2007

Transformed hairy roots were induced from in vitro grown plantlets of Trichosanthes kirilowii by infection with Agrobacterium rhizogenes strain ATCC15834. Transformed hairy roots exhibited active growth with high branching of roots on plant growth regulators-free medium. Cloned line (TR-03) of hairy root was tested for its growth and extracellular protein accumulation in medium under various culture conditions. Among the culture media tested, a full-strength MS medium had a pronounced effect on root biomass and extracelluar protein accumulation in medium. The maximum root biomass (2.4 g DRW/flask) and extracellular total protein contents $(28.3ug/m\ell)$ in medium was obtained at inoculum size of 2 g (FRW) and in MS medium supplemented with 4% sucrose. In addition, the optimal shaking speed for root growth and extracellular protein accumulation in medium were 100 rpm. The total extracellualr protein concentration reached a maximum of $28.3ug/m\ell$ at 4 weeks and decreased thereafter. Protein translation inhibitory activity was observed in culture broths and reached levels of 21.3 unit. These studies demonstrate that the transformed hairy roots can be utilized for the in vitro production of ribosome-inactivating proteins.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.324-327
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• 2009

The white flowered 'Eorayeon 1ho' is the first Kangwon National University cultivar of L. ${\times}$ formolonga, commonly known as seed propagating lily. Crosses were conducted from 1999 to 2003 among the selected selfing lines($S_3$) of L. longiflorum 'Lorina', L. ${\times}$ formolonga 'Augusta' and 'Raizan Herald'. A new cultivar was preliminarily selected as 'E-No.1' in 2003 for its stable upward flowering habit. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 1ho' blooms in early July at outside, with high resistance to Botrytis and having upward flowering habit. The plant height is 112.4 cm and the percentage of seed germination is 96.4%. This seed propagating cultivar shows good quality as a cut flower and low blasting rate.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.67-67
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• 2019

뽕나무과(Moraceae) 닥나무속(Broussonetia)의 품종에 대한 국내 연구동향으로는 잎의 성상이나 암꽃의 포길이, 엽신의 폭과 같은 수목학 분수학적 관점으로 특징을 파악하기 때문에 구분이 어려운 실정이다. 또한 닥나무(Broussonetia kazinoki Siebold)와 꾸지나무[Broussonetia papyrifera (L.) $L^{\prime}H{\acute{e}}r.$ ex Vent.]의 수목학적 특징이 유사하여 오동정의 사례가 발생하기도 한다. 닥나무의 향명은 다양하게 불리지만 그 분류기준은 명확하지 않다. 본 연구에서는 총 4개 마커(ITS, matK, trnL-F, ndhF)의 염기서열 분석과 분자계통학적 분류를 통해 닥나무와 꾸지나무의 교잡종으로 확인된 전라도지역 닥나무 8개체에 대하여 펄프제지 자원으로써의 특성을 확인하였다. 8개체 인피섬유의 형태학적 특징인 섬유장, 섬유폭, 섬유 내강(Lumen)폭을 통해 Runkel ratio, Slenderness ratio, Flexibility coefficient, Rigidity coefficient를 도출하고 종이의 기계적 강도, 내절도와의 상관관계, 섬유와 섬유간의 결합력 등을 예측하였다. 상기와 같은 연구결과는 유전형질이 동일한 전라도지역 닥나무의 형태학적 특성을 통해 펄프제지 공정에 영향을 미칠 수 있는 인자들을 분석 하였다. 이러한 특성에 따라 다양한 품질의 종이를 제조 할 수 있는 기초 자료로 제공될 것이다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.6
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• pp.539-542
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• 1990

This experiment was conducted to obtain some informations on the effects of number of leaf harvest and time to agronomic characters and grain yield in perilla. For the experiments perilla seedlings of 40days old were transplanted on June 20, July 20 and August 20 in 1988, and leaf harvested at 30. 40. 50 and 60 days after transplanting, respectively. The following results were obtained from the experiments. In the case of the fully developed leaves were harvested, culm length were decreased by more harvests and by the later harvests. However culm diameter, number of nodes and branches were little influenced by the leaf harvests. Number of clusters, weight of 1,000 grain and grain yield were significantly influenced by the number of leaf harvest and time. As the above results, considering the grain yield, leaf harvest would be available in the case of once or twice at the early growing stage.

• Korean Journal of Plant Taxonomy
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• v.44 no.3
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• pp.178-180
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• 2014

We report a new record of Jasminum albicalyx Kobuski in Vietnam. The plant differs from J. pedunculatum Gagnep., a closely related taxon, by foliar and floral characters such as less number of lateral veins, presence of domatia at vein axils on the abaxial surface of leaf, smaller bracts, white calyx having more number of lobes and less number of lobes in corolla. Morphological description, line drawing and color photographs are provided in addition to the species key of allied species of Jasminum albicalyx in Vietnam.