• Journal of Ginseng Research
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• v.14 no.2
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• pp.191-199
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• 1990

It has been well known that extended exposure to reactive oxygens causes severe damage to susceptible biomolecules. In this study, the effects of flavonoids including trifolin and kaempferol from Ginseng leaves on singlet oxygen induced photohemolysis of erythrocytes and free radical scavenging activities were investigated. Each flavonoid aglycone (5-50$\mu$M) such as kaempferol, quercetin or baicalein exhibited a high protective effect against the photohemolysis. They protected the cells by scavenging $^1O_2$ and free radicals Although the free radical scavenging activities of the flavonoid glycosides were not much lower than those of their corresponding aglycones, their insolubility into lipid bilayers of membrane made them less effective in preventing the photohemolysis induced by $^1O_2$. The $^1O_2$ and free radical scavenging activities of flavonoids were estimated by the decomposition of the flavonoid by $^1O_2$ and the bleaching of free radicals by the flavonoid, respectively. The solubilization of the flavonoid into micells or erythrocytes was deduced from spectrophotometric and microscopic observations. The cooperation of L-ascorbic acid and a flavonoid, and a possible involvement of lipoxygenase or cyclooxygenase in the photohemolysis mechanism were discussed.

• Journal of Ginseng Research
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• v.11 no.1
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• pp.39-45
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• 1987

Panu ginseng (6 Year old) was grown $17^{\circ}C$/$15^{\circ}C$ and $27^{\circ}C$ day/$23^{\circ}C$ night in the light room of phytotron for 84 days. The composition of neutral lipid(NL), glycolipid(GL), phospholipid(PL) and fatty acids were investigated in leaves. The contents of NL and GL were higher in $25^{\circ}C$ while PL was lower. Similarity (simple correlation) of lipid composition between $16^{\circ}C$ and $25^{\circ}C$ was not significant for PL and GL but significant for NL(p = 0.001), indicating that PL and GL were important factors in the mostability. Similarity of fatty acid composition between growth temperatures was highly significant (p = 0.001) for all three lipids, while similarity between lipids was significant between NL and PL (p=0.01) and NL and GL (p=0.05), but nonsignificant between GL and PL at $16^{\circ}C$. .In NL digalactosyldiacylglycerol (3->$7^{\circ}$) increased but monogalactosyldiacylglycerol (10%) did not change at $25^{\circ}C$. In PL phosphatidic acid (22 -> 4%) and phosphatidylinositol (18 -> 5%) decreased but phosphatidyl ethanolamine (12->l6%) increased at $25^{\circ}C$. Percent unsaturated acid slightly decreased in NL and PL but greatly increased in GL at $25^{\circ}C$. Percent unsaturated bond slightly decreased in NL but did not change in PL and GL.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.386-391
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• 1987

Effect of growth light intensity (5% and 20%) and planting density (40, 56, 90/33㎡) on yield and red ginseng quality was investigated in a sandy loam field of ginseng plantation. High growth light intensity had no effect on yield and quality but decreased stem length, leaf area and chlorophyll content and increased specific leaf weight. The optimum planting density was 64/3.3㎡ for both yield and Heaven + Earth grade (HE grade) of red ginseng. HE-shape ginseng rate showed significant positive correlation (P=0.0l) with HE grade rate.

• Journal of Ginseng Research
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• v.19 no.3
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• pp.275-280
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• 1995

The formation of thylakoid membrane proteins and changes in the chloroplast ultrastructure of ginseng leaf were investigated as a function of time following the leaf emergence. The leaf chloroplast obtained just after the leaf emergence showed short rod-like thylakoids which were connected and arranged in 3~4 layers along the longitudinal axis of the chloroplast. The 10 DAE (days after emergence) chloroplast started to form grana structure. The typical grana structure was observed 17 DAE, and the grana was fully developed 28 DAE. The membrane proteins obtained from just after emerging leaf were separated into many minor bands indicating no CP-complex formation yet. LHC II was detected after 10 days. CP 47 and CP 43 were detected after 17 days. After 28 days, the PS I and PS II proteins were distinctly separated into CP 1, LHC II, CP 47, CP 43, CP 29, CP 27+24. Thus, the appearance of the light harvesting protein, LHC II, which was concentrated in grana stacks, was consis tent in time with the formation of grana stacks 17 DAE. Key words Chloroplast ultrastructure, grana, CP-complex, LHC II.

• Journal of Soil and Groundwater Environment
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• v.22 no.5
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• pp.128-134
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• 2017

This work presents the adsorption capability of green tea and ginseng leaves to adsorb heavy metals such as Cd(II), Cu(II), and Pb(II) in aqueous solution. FT-IR analysis indicates the presence of oxygen containing functional groups (carboxyl groups) in two kinds of leaves. High pH condition was favorable to the adsorption of heavy metal ions due to the enhanced electrostatic attraction and the precipitation reaction of metal ions. The adsorption of Cd(II), Cu(II), and Pb(II) reached equilibrium within 10 min, achieving high removal efficiencies of 80.3-97.5%. The adsorption kinetics data of heavy metal ions were fitted well with the pseudo-second-order kinetic model. The maximum adsorption amounts of Cd(II), Cu(II), and Pb(II) ions were 8, 3.5, and 15 mg/g, respectively, in the initial concentration range from 0.15 to 0.75 mM. Based on the fitting data obtained from isotherm models, heavy metal adsorption by green tea and ginseng leaves could occur via multi-layer sorption.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.3
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• pp.286-292
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• 1986

For the elucidation of saponin synthesis during ontogeny changes of ginsenosides and free sugars in seeds during stratification and seedlings in early growth stage were investigated with high performance liquid chrom-atography. Embryo plus endosperm at 40-day stratification showed 80% decrease of total saponin, disappear-ance of Rc, Rb$_2$ and Rb$_1$ and appearance of Rg$_3$ (probable) and 20-Glc-Rf (probable). Leaf ginsenoside F$_3$ was found not in fruit plup but seed and decreased during stratification. Both decomposition and synthesis of saponin seemed to occure during stratification. Ginsenosides in endosperm and embryo might be originated from fruit pulp by penetration. In seedling saponin appeared first in shoot and in root about one month later. Ginsenoside Rc, Rb$_2$, Rb$_1$ appeared in root at the last investigation (June 30) indicating normal saponin synthetic capacity of root. Saponin synthetic rate was twice in leaf than in root. Leaf ginsenoside F$_3$ was found in seedling root. Root saponin Rg$_3$ and 20-Glc-Rf were found in leaf and stem in seedling and decreased with growth suggesting that rate saponin is not such in certain growth stage. Total saponin content was negatively correlated with PT/PD in seeds and arial parts of seedling due to greater change of PD. than PT. Seed at 70days stratification showed high sucrose content. In seedling glucose was main sugar in stem all the while and sucrose in root at early stage while glucose, fructose and sucrose were found in leaf.

• Korean Journal of Soil Science and Fertilizer
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• v.12 no.2
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• pp.77-82
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• 1979

Wilting and transpiration charactistics of Panax ginseng leaves were investigated at two temperature levels. Water potential and water absorption characteristics of leaf segments were also observed. Soybean leaves were compared. 1. Ginseng leaves were thiner, higher in water content and lower in dehydration rate. But time required to reach permanent wilting point (pwp) was less than half of that of soybean leaves because water content at pwp was about two times higher (80% of initial water for ginseng and 50% for soybean leaves). The time was shorter under high air temperature. 2. Transpiration rate was about a quater of soybean leaves and lower at $33^{\circ}C$ than $23^{\circ}C$, indicating that ginseng leaves are less tolorant to high air temperature. 3. Ginseng leaf segment showed smaller water free space but greater water deficit and little difference in was absorption rate. 4. Water potential of leaves measured by liquid immersion method was lower than that of soybean leaves. 5. Above results strongly suggest that ginseng plants are more susceptible to water stress. Thus greater light intensity during leaf growing stage (April to June) is recommendable to increase stomate frequency resulting greater transpiration rate and high temperature tolerance during July and August. Abundant water around roots seems to be beneficial as long as oxygen is not limited in rhizosphere.

• Journal of the Korea Convergence Society
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• v.8 no.8
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• pp.191-196
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• 2017

This study was conducted to investigate the effect of fine bubble water treatment on the growth of ginseng seedlings. The growth of ginseng seedlings which were treated with general water and fine bubble water was investigated. The above ground part, the growth of leaf was increased by about 10% and the growth of the stem was about 6%, as the ginseng seedlings which were treated with fine bubble water comparing to the ginseng seedlings treated with general water. Root length was increased about 5%, root width was increased about 8%, roots weight was increased about 9%, and dry weight was increased about 7%. This is not because the whole root growth was increased, but the main root growth was increased about 7%. These results suggested that the physical properties of the fine bubble water stimulated the growth of ginseng seedlings. These results are initial study in the case of ginseng seedlings. Therefore, it can be applicable to the 3-5 years old ginseng plants. Further research will be needed to find out the optimal cultivation condition by controlling the dissolved oxygen amount according to the soil condition and the research applied to the ginseng seedlings.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.165-168
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• 1989

Ginseng plants in the 2nd year were grown under the shade (90-95%) of colored cellophane films at $15^{\circ}C$ in a natural light phytotron. Relative root grown (final/initial) was in decreasing order of blue, green, white, yellow and red. The growth of aerial part, especially stem length was poorest in blue light with relative root growth negatively correlated with stem length. In this relation each color showed specific domain. Stem length showed positive linear correlation with leaf area per plant in each and among light though it was not significant in red light. The content and ratio of chlorophyll a and b in leaf were in the decreasing order of White, Blue, Green, Red and Yellow. There is positive correlation between chlorophyll a and chlorophyll b, and between total chlorophyll and chl:a/b ratio. Blue color shade is expected to be beneficial for ginseng production.

• Journal of Mushroom
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• v.16 no.3
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• pp.170-174
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• 2018

This study aims to investigate the antifungal and protective effects of water- and 70% methyl alcohol-extracts from spent mushroom substrate (WESMS and MeOHSMS) of Lentinula edodes, on Botrytis cinerea- the causative agent for gray mold disease in ginseng. MeOHSMS inhibited mycelial growth and spore germination of Botrytis cinerea, by 75% and 95%, respectively. MeOHSMS could suppress gray mold disease of ginseng seedlings by 80% and effectively reduce the disease severity by 60%. Compared to the treatment of ginseng leaves with WESMS and DL-${\beta}$-aminobutyric acid (BABA), the MeOHSMS treatment increased the phenolic compounds in the leaves by 36% and 18%, respectively. These results suggest that the SMS extracts suppress gray mold disease in ginseng via dual functions: antifungal activity and increase in a plant defense factor-phenolic compounds.