• Korean Journal of Poultry Science
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• v.30 no.2
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• pp.91-94
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• 2003

Artificial insemination of cryopreserved semen is not practiced in broiler breeder industry although its benefit has been recognized for years. Thus, the present study explored the possibility of artificial insemination with cryopreserved semen in Ross broiler breeders. Sixty Ross female broiler breeders were artificially inseminated either with fresh or with cryopreserved semen pooled from five male Ross broiler breeders. On.e hundred eighty eggs were collected from each semen treatment and hatched. Fertility and hatchability of fertilized eggs collected from hens artificially inseminated with cryopreserved semen were 68.0 and 47.1％, respectively, compared to 88.3 and 87.2％ with fresh semen. Both unfertilized eggs and dead embryos during hatch were significantly (P<0.05) higher in the cryopreserved semen­treated group by approximately 2.7­ and 3.2­fold than the fresh semen­treated counterparts. the moderately low fertility and markedly low hatchability of fertilized eggs obtained from cryopreserved semen observed in this study may be of practical interests in broiler breeder industry and deserves further study.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.401-406
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• 2008

This study was conducted to clarify the characteristics of estrus-related vocalization of sows after artificial insemination. Vocalization of sows in artificial insemination day, and 3 days and 50 days after artificial insemination, were recorded 3 hours per day from September 2006 to March 2007 using the MD Recorder(Marantz PMD-650) and microphone(RF Condesner MIC, MKH 416P48). The shapes of spectrum and spectrogram of vocalization were different in each period after artificial insemination. There were significant differences in frequency and intensity, but not in duration of vocalization. The fact that signal may give a reliable indication of the signaller's needs has suggested that in some circumstances they can provide information on animal welfare.

• Journal of Animal Science and Technology
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• v.49 no.4
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• pp.451-458
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• 2007

This study was to investigate the effectiveness of pre-weaning injection of gonadotropin and scheduled fixed-time insemination on sow fertility. Sows were randomly assigned to receive gonadotropin 2 days before weaning, 1 day after weaning or none as control. In gonadotropin treated groups, half of sows were inseminated twice at 24 and 36 hours after onset estrus and half of sows were inseminated twice at scheduled fixed-time. Weaning to onset of estrus was the shortest in lactating sows injected with gonadotropin prior to weaning. Liter size was significantly higher in AI groups after detection of estrus, compared to fixed-time AI group. Results of these experiments indicated that injection of gonadotropin in lactating sows could initiate the final follicular development, and has potential to enhance the reintegration of estrus. Further researches are needed to define the relationship between reduced interval of wean to onset estrus and enhanced fertility in lactating sows.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.10
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• pp.446-451
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• 2019

This study investigated the application of artificial insemination and pregnancy diagnosis kit for Korean native black goats. Semen was collected by electrical ejaculation, followed by semen analysis and artificial insemination in three goat strains (Dangjin, Jangsu and Tongyoung). Pregnancy was confirmed using a cow pregnancy test kit (IDEXX Rapid Visual Pregnancy Test kit) and ultrasound diagnosis. Analysis revealed that semen collected from male Korean native black goats by electrical ejaculation was about 1~1.5 ml in volume, $18{\sim}25{\times}10^8/ml$ concentration, and having > 97% motility. Furthermore, confirmation of pregnancy by pregnancy test kit and ultrasound diagnosis after artificial insemination were similar. In addition, the efficiency of pregnancy was 20~40% for all three strains: Tongyoung was the highest with 44%, followed by Dangjin (%), and Jangsu (20%). This study determines the artificial fertilization efficiency and the feasibility of using a cow pregnancy test kit for early pregnancy diagnosis in Korean native black goats. Although further research is required for validation, the results of the current study contribute to the breeding and improvement of Korean native black goat in research institutions as well as in general farms.

• Journal of the Korean Chemical Society
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• v.62 no.1
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• pp.30-35
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• 2018

The purpose of this study was to decrease the protein adsorption and improve the function of the hydrophobic acrylic Intraocular lens(IOL). Hydrophobic acrylic intraocular lenses were prepared by using ethyleneglycol phenyletheracrylate (EGPEA), styrene and 2-hydroxyethyl methacrylate (HEMA). Polyvinyl pyrrolidone (PVP) and 2-methacryloyloxyethyl phosphorylcholine (MPC) were used as additives. Water contents, wettability, light transmittance and protein adsorption amount were measured to evaluate the physical properties of the intraocular lens. The water content and wettability of all samples containing additives were increased and the amount of protein adsorption decreased. In particular, samples containing MPC showed a further decrease in protein adsorption. The hydrophobic acrylic intraocular lens with PVP and MPC was found to improve the function of the intraocular lens by reducing the protein adsorption while having basic physical properties.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.290-290
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• 2004

유전자재조합 소 소마토트로핀(recombinant bovine somatotropin, rBST)은 인슐린양성장인자(IGF)-1의 합성과 분비량 조절에 의해 당과 아미노산의 흡수 및 단백질합성의 촉진을 통한 체내흡수 영양소 이용효율 증가, 산유량 증진 및 증체 효과, 세포증식의 촉진작용 등이 있는 것으로 보고(Bauman, 1982; Mears, 1995; 권 등, 1997)된 바 있으며, 또한 최근에 젖소 및 육우빈우의 인공수정시 rBST를 투여하였을 때 1회 수정수태율, 혈중 progesterone 농도 및 IGF-1 농도 등에 영향을 미치는 것으로 보고하였다(Lucy 등, 1994; Bilby 등, 1999; Morales-Roura 등, 2001). (중략)

• Clinical and Experimental Reproductive Medicine
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• v.37 no.2
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• pp.169-172
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• 2010

Retrograde ejaculation is a condition that causes male infertility. Infertiltiy treatment is usually based on assisted reproductive technology with the use of sperms recovered from the bladder after ejaculation. Many pregnancies have been tried by artificial intrauterine insemination with the husband's sperm recovered from voided urine. In this case, ovulation was induced by clomiphene citrate and human menopausal gonadotropin, pH and osmorality of urine was controlled by modified Ham's F-10 contained 10% serum substitute supplement and immediately semen collection, to improve sperm motility. We had experienced a successful pregnancy case by above method, and reported with brief review of literature as well.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.87-87
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• 2003

본 연구는 개의 수태율 향상을 위한 인공 수정적기를 구명하기 위하여 발정개시 후 초음파를 이용하여 난소내 난포발달 상태를 진단하였다. 자연적으로 발정이 개시된 Greyhound 2두에 대해서 초음파기(SA 600S, 메디슨, 한국)와 3.SMHz probe를 이용하여 난포발달에 대한 초음파화상을 얻었다. 외음부가 종창하고 출혈이 확인된 첫날을 발정개시일(Day 1)로 하였으며 발정개시 후 11일째, 13일째 및 15일째 난포발달 상태를 조사한 결과 좌우 난소에 각각 3개 및 4개의 난포가 발달중에 있었으며, 난포의 크기는 좌우 각각 11일째에 6, 9, 17mm(좌측)와 6, 9, 6, 5mm(우측)였으며, 13일째에는 각각 10, 11, 11mm 및 9, 14, 13, 12mm였다. 15일째에는 이미 배란이 일어나서 난포상을 관찰할 수 없었으나 배란흔적은 관찰할 수 있었다. 본 실험에서는 발정개시 후 11, 13, 15일째 0.25$m\ell$ 주입기를 이용하여 그레이하운드 동결정액으로 인공수정하였으며, 이때 동결정액(좋은유전자 Co.)의 융해 후 활력 있는 정자수는 3$\times$$10^{7}$ spermatozoa/dose(0.25$m\ell$)였으며, 자궁경관내 주입기선단의 관통 여부를 촉진한 후 자궁내 정액 주입을 확인할 수 있었다. 난포발달에 대한 초음파 진단결과 Greyhound에 있어서 1회 인공수정시에는 발정개시 후 15일째가 높은 수태를 위한 수정적기라고 사료되었으며 이러한 결과는 Concannon 등(1989)이 배란 후 2일째가 수정적기로 판단한 연구 결과와 유사한 경향을 나타내었다.

• Clinical and Experimental Pediatrics
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• v.50 no.8
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• pp.740-745
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• 2007

Purpose : There is a dominant opinion that in vitro fertilization (IVF) leads to an increased incidence of twins, low birth weight (LBW) infants, prematurity and mortality. On the other hand, technical development of IVF and improvement of neonatal intensive care have increased the survival rate of neonates. The purpose of this study was to verify the tendency by comparing the clinical aspects of IVF and spontaneously conceived twins, and to establish methods to increase the survival rate of neonates after IVF. Methods : Retrospective reviews were performed on all twin infants who were admitted to the nursery and NICU at Kangnam Sacred Heart Hospital, Hallym University from January 1, 2000 to December 31, 2006. Medical records of IVF twins (study group, n=92) and spontaneously conceived twins (control group, n=265) were analyzed and compared. Neonatal outcomes and complications, as well as obstetric outcomes, were analyzed and compared. Results : Mean gestational age and birth weight of the study group ($34.6{\pm}3.5$ weeks, $2,203.9{\pm}617.2g$) were considerably lower than those of the control group ($36.3{\pm}2.4$ weeks, $2,367.0{\pm}517.9g$). The frequency of prematurity less than 37 weeks (68.5% vs 51.3%) and extremely LBW (15.2% vs 6.4%) were also significantly higher in the study group. Other neonatal outcomes were all insignificant. The obstetric characteristics, maternal age ($32.6{\pm}3.3$ years vs $30.3{\pm}3.9$ years) and the frequency of cesarean delivery(95.7% vs 79.9%) were significantly higher in the study group. Other obstetric outcomes were insignificant except for the frequency of incompetent internal os of cervix (36.2% vs 3.6%) and cerclage operation (38.3% vs 4.3%). Conclusion : Based on the above results, clinical outcomes of twin infants will be further improved by careful attention and thorough antenatal care of the IVF twins.

• 한국가축인공수정사협회:학술대회논문집
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• v.1991 no.05
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• pp.24-27
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• 1991