• Korean Journal of Environmental Agriculture
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• v.15 no.2
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• pp.217-222
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• 1996

A green-house experiment was conducted to know the performance of Chinese cabbage and radish applied with simulated acid rain(SAR) on the leaf and/or soil. The pHs of SAR and normal water were 2.7 and 6.0, respectively. The pHs of SAR and normal water applied on leaf/soil were 6.0/6.0, 6.0/2.7, 2.7/6.0, and 2.7/2.7 and they were applied fifteen times at the two- or three-day intervals with 10mm at a time. Leaf application of SAR caused brown spots in both crops, while no such symptoms were observed in soil application. SAR applied on the leaf or soil increased chlorophyll content significantly in Chinese cabbage, but slightly in radish. Leaf or soil application of SAR did not affect N, P, and K concentrations in both crops. The sulfur content in radish leaf increased by leaf or soil application of SAR, while in the root it increased by soil application only. Yield of Chinese cabbage was not affected by SAR, while both leaf and root yields of radish were significantly reduced by leaf application of SAR compared with soil application.

• Korean Journal of Weed Science
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• v.31 no.2
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• pp.152-159
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• 2011

The effects of simulated acid rain (SAR) on fatty acid composition and biochemical defense responses of plant was investigated. Garden balsam (Impatiens balsamina L.) was exposed to four pH levels (5.6, 4.0, 3.0, 2.0) of SAR and placed in the growth chambers for 2 weeks. SAR drastically inhibited chlorophyll content of garden balsam. The level of $H_2O_2$ was significantly increased by SAR. As pH levels decreased from 5.6 to 2.0, the ratio of unsaturated to saturated fatty acids of garden balsam was increased. Changes of three major polyamines (putrescine, spermidine and spermine) of garden balsam leaves were observed. All of the polyamine contents were increased with SAR. Catalase activities of the plant affected by SAR were increased as the pH decreased. The results indicate that the application of SAR generates oxidative stresses from the garden balsam and retards the plant growth significantly. A biochemical protect mechanism might be activated to neutralize the oxidative stresses generated through SAR.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.889-894
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• 2005

This experiment was carried out to investigate the effects of simulated acid rain (SAR) in the shoot growth and internal structure of Arabidopsis thaliana. In the shoot growth, the plants treated with SAR (pH 3.0) for 15 days showed no morphological change compared to the control plants. Some change was observed in the internal structure of the stems: the epidermis and cortex tissues of the stems treated with SAR were partly damaged. The plants treated with SAR showed no noticeable difference compared to the control plants, but morphological changes were observed in the leaf. The leaves of the plants treated with SAR showed many white necrotic spots on the part of upper epidermis. A light microscopic examination of the leaves with necrotic spots showed that the upper epidermis was severely compressed with the damaged cuticle layer and the mesophyll cells were also damaged and compressed. However, noticeable structural change of vascular bundle cells was not observed.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.335-340
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• 1996

A green house experiment was conducted to investigate the growth of Chinese cabbage, radish and soybean and change in the chemical properties of the soil after the 10mm application of the simulated acid rain(SAR) of pH 2.7 in the form of sprinkle and mist. It was applied 30 times for Chinese cabbage and radish and 62 times for soybean at the two-day intervals. The results obtained are summarized as follows: 1. Visual damages caused by SAR were dark-brown or red brown leaf spots in Chinese cabbage, and dark-brown and wrinkled leaf margins in radish and soybean. 2. The degree of visual damages became severer as the number of SAR applications increased and it was severer with mist than with sprinkle of SAR. 3. Chlorophyll content was reduced by SAR, but it was not affected by the form of SAR application(sprinkle or mist) in all crops, although it was slightly lower with mist than with sprinkle of SAR. 4. Fresh weight of Chinese cabbage heads and radish roots and grain yield of soybean were reduced by SAR, and were not affected by the form of SAR application. 5. Contents of K, Ca, and Mg in leaves were reduced, while S content increased by SAR in all crops. The forms of SAR application did not affect contents of mineral nutrients in all crops. 6. SAR decreased soil pH and the contents Ca, Mg, and K of soil, while increased $SO_4$ content. However, the contents of soil organic matter, N, and P were not affected by SAR. Forms of SAR application did not affect soil chemical properties either.

• Korean Journal of Environmental Agriculture
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• v.15 no.2
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• pp.207-216
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• 1996

Simulated acid rain(SAR) treatment caused a lethal effect on the newly germinated seedlings of Capsicum annum cv. Komyung even though the germination rate of the seeds was stimulated by the treatment of SAR. Young germinated seedlings were much more sensitive to SAR than the already-grown seedlings. The typical symptom caused by SAR was white spot or lesion on the leaf and appeared readily and severely at the low pH of SAR. Generally, Phytophthora rot was severer in SAR treatments than in control. However, the disease was less severe in lower pH of the SAR treatment than in higher pH. Pathogen inoculation following the SAR treatment increased the disease. The severest Phytophthora rot was observed in the SAR treatment of pH 4.0 or 4.5 regardless of the inoculation time.

• Journal of Plant Biology
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• v.37 no.1
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• pp.93-99
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• 1994

은행나무와 곰솔의 유식물의 산성비에 대한 생장에 미치는 영향을 알아보기 위하여 pH 5.6을 대조구로 하여 pH 4.0, 3.2와 2.4의 인공산성비를 3개월간 처리하여 생장량, 엽록소 함량과 탄수화물의 함량을 조사하였다. 은행나무와 곰솔의 유식물은 pH2.4에서 생장이 가장 억제되었으며 반면에 pH 3.2에서는 생장이 촉진되었다. 전체적인 생장량은 뿌리에 비해 지상부의 생장에서 산성비의 영향을 더 받았다. 엽록소 함량은 산성비의 산도에 따라 중요한 차이가 없었으며 은행나무 잎의 탄수화물의 함량은 포도당과 설탕의 함량이 pH 4.0에서 가장 낮았고 전분의 함량은 pH 3.2에서 가장 낮았다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.1
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• pp.56-60
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• 1997

In order to investigate the relationships between the responses of four crops (rice, soybeans, potatoes, and red pepper) to acid rain, the simulated acid rain(SAR) of pH 1.8, 2.3, and 2.8 and normal rain of pH 6.0 were applied from 30 days after emergence to harvest at the two-week interval for rice, soybeans and red pepper and at a week interval for potatoes. SAR of pH 1.8 and 2.3 caused brown spots in the leaves of rice, soybeans, and red pepper and the damages were severer as the pH of SAR lowered, while no visual damages were observed in potatoes. The SARs did not affect chlorophyll content of rice and potatoes, while chlorophyll content of soybeans and red pepper decreased as the pH of SAR lowered. Photosynthetic activity was not significantly different among the pHs of SAR in rice, while decreased as pHs of SAR lowered from pH 2.8 to 1.8 in soybeans, potatoes, and red pepper. Yield of rice, soybeans, and potatoes was not affected by the pHs of SAR, but in red pepper the length, diameter, and weight of a fruit, the number of fruits per plant, and total matured fruit yield decreased as the pHs of SAR lowered. In rice and potatoes visual damages caused by SAR did not correlated with other observed traits. However, visual damages were negatively correlated with chlorophyll content and photosynthetic activity of soybeans and photosynthetic activity and yield of red pepper.

• Korean Journal of Environmental Agriculture
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• v.16 no.2
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• pp.175-180
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• 1997

This experiment was conducted to find out the effects of lime materials application on reducing injury of simulated acid rain(SAR) in soybean grown in pot contained with sandy loam. Six treatments including control, slaked lime(SL), 1% and 2% lime water(LW) and composite treatments with SL+LW were applied. Slaked lime was applied to soil in pot before planting, and lime water was applied to leaves a day prior to the spray of SAR(pH 2.7) and normal rain(pH 6.0), and these were sprayed at 2-day intervals. Growth, yield and yield components, foliar injury rate, chrolophyll content and photosynthetic activity in leaves, content of mineral nutrients in plant and soil chemical properties were analyzed and investigated. These results obtained are summarized as follows : Seed yield of all lime treatment was reduced by SAR compared with control. But seed yield of all lime treatment was increased with treatment of lime material in soil and on leaves. After 15 and 45 times spray of SAR, all lime treatments were effective in injury reducing visible injury of leaves compared with none treatment. Chlorophyll content in leaves was highest in plants treated with slaked lime+ 1% lime water and photosynthetic activity was highest with treatment of slaked lime. Concentration of total nitrogen, phosphate, and sulfur in soybean plant were increased by the spray of SAR. Concentration of total nitrogen, potassium and calcium in soybean plant were increased with treatment of slaked lime into soil. By treatments of SAR, soil pH was decreased, and total nitrogen and sulfur concentration in soil were increased. However, available phosphate and exchangeable cations in soil such as calcium, magnesium and potassium were reduced. Soil pH, calcium and silicate concentration were increased with treatment of slaked lime into soil.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.05a
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• pp.257-258
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• 2003

애기장대를 실험재료로 사용하여 인공산성비의 주요 성분인 황산과 질산 중 어느 성분이 식물 생장과 종자 발아에 영향을 미치는가를 조사하였다. 질산과 황산성분이 모두 첨가된 인공산성비를 처리한 애기장대는 정상식물에 비하여 줄기와 뿌리 생장이 각각 28%, 30% 정도 감소되었고, 잎 표면에는 많은 괴사반점이 관찰되었다. 인공질산비를 처리한 식물은 정상식물에 비하여 줄기와 뿌리 생장이 각각 14%, 17% 정도 감소된 반면 인공황산비를 처리한 식물은 각각 24%, 25% 정도 감소되었다. 종자의 발아율은 인공산성비 배지에서는 80%, 인공황산비 배지에서는 88%, 인공질산비 배지에서는 93%로 나타났다. 이와 같이 성분이 다른 3 종류의 인공산성비를 애기장대에 처리하였을 때 식물 생장과 종자발아율은 질산보다 황산성분에 의해서 더 큰 영향을 받는 것을 확인하였다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.556-563
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• 1994

To compare the tolerance of crops to acid rain of simulated acid rains(SAR) of three pH levels (3.0, 4.5, and 6.0) were applied to rice, corn, soybean, adzuki bean, hot pepper, tomato, sesame, barley, wheat, Chinese cabbage, radish, lettuce, and spinach for 30 days from 20 days after emergence at two-day interval with 10mm at a time. No visual damages were observed. Also, no alteration of ultrastructure was observed although some lightly stained lipid granules were observed in the chloroplasts of sesame, soybean, and adzuki bean at the SAR treatment of pH 3.0. As pH of SAR decreased, chlorophyll content increased in adzuki bean, decreased in rice, tomato, and spinach, and similar in the other crops. Photosynthetic activity of adzuki bean increased, while decreased in Chinese cabbage and barley as pH of SAR decreased, and similar in other crops. Concentration and uptake of N were not affected by SAR treatments in all crops except tomato and barley. When a strong SAR of pH 2.0 was applied, rice, corn, sesame, tomato, barley, and wheat were relatively tolerant, while adzuki bean, hot pepper, soybean, Chinese cabbage, lettuce, radish, and spinach were susceptible in terms of visual damages.