• Research in Plant Disease
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• v.30 no.1
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• pp.66-77
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• 2024

Black wood ear mushroom (Auricularia auricula-judae) is one of the most economically important mushrooms in China, Japan, and Korea. The cultivation of wood ear mushrooms on artificial substrates is more efficient in terms of time and cost compared with their natural growth on trees. However, if the substrate cultivation is infected by fast-growing fungi, the relatively slow-growing ear mushroom will be outcompeted, leading to economic losses. In this study, we investigated the competitive fungal isolates from substrates infected with fast-growing fungi for the cultivation of ear mushrooms in Jangheung and Sunchon, Korea. We collected 54 isolates and identified them by sequencing their internal transcribed spacer region with morphological identification. Among the isolates, the dominant isolates were Trichoderma spp. (92.6%), Penicillium spp. (5.6%), and Talaromyces sp. (1.8%). To find an appropriate eco-friendly biocontrol agent, we used five Streptomyces spp. and Benomyl, as controls against Trichoderma spp. and Penicillium spp. Among the six Streptomyces spp., Streptomyces sp. JC203-3 effectively controlled the fungi Trichoderma spp. and Penicillium spp., which pose a significant problem for the substrates of black wood ear mushrooms. This result indicated that this Streptomyces sp. JC203-3 can be used as biocontrol agents to protect against Trichoderma and Penicillium spp.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.127-127
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• 2003

Haptophyta(이하 하프토조로 표기)는 그 일부종이 toxin을 가지며, 더욱이 이들 종들이 원인 생물로 발생하는 bloom으로 인한 경제적 피해 발생 등으로 인해 최근 여러 나라에서 활발한 연구가 이루어지고 있다. 남해안 일대의 몇몇 어ㆍ패류 양식장에서 이용하고 있는 식물먹이생물 및 동물먹이생물의 배양수조 내에서 배양을 목적으로 하는 먹이생물 이외의 혼재 생물의 종류를 조사하던 중 Haptophyta의 Prymnesium속 생물이 혼재하고 있는 것을 발견하였으며, 혼재된 Prymnesium은 양식 패류의 인공 종묘 생산 과정에서 사육 치패의 먹이로 이용되는 식물먹이생물 Isochrysis galbana의 증식을 크게 억제하였고, 더욱이 Prymnesium이 혼재된 Isochrysis galbana를 인공 종묘생산 중이던 치패에 투이한 결과 치패 모두가 폐사에 이르는 것을 관찰하였다. 이에 Isochrysis galbana의 배양 수조내에 Prymnesium이 혼입 하였을 경우 Isochrysis galbana의 증식양상을 알아보기 위해, Isochrysis galbana의 세포밀도를 5,440,000cells/ml로 하고 Prymnesium의 세포밀도를 10,000cells/ml로 하여 혼합 배양한 결과 배양개시 1일 후의 각 세포밀도는 Isochrysis galbana가 1,040,000cells/ml로 급격히 저하하였으며, 이와는 달리 Prymnesium은 50,000cells/ml로 증가하였다. 이들 종 각각의 증가 및 감소추세는 계속되어 배양개시 5일 후에는 Isochrysis galbana가 530,000cells/ml로 처음 배양당시 세포수의 90% 이상 감소하였고, Prymnesium은 43,333cells/ml로 4배 이상 증가하였다. 이 실험에서 Isochrysis galana의 세포수가 감소하는 이유는 정확히 알 수는 없으나, 하프토조의 일부 종에서는 광합성을 통한 유기물합성 이외에도 외부로부터의 DOC(dissolved organic carbon)를 직접 배양수로부터 취하거나 또는 영양염 제한 조건에서는 food particle을 섭취하는 경우가 있는 것으로 보고되고 있다. 한편, 이들 Haptopyta가 양식장에서 이용되어지는 해수를 통해 유입되는지를 알아보기 위해 모래여과조를 통과한 해수를 플랑크톤 배양용 배지의 첨가 없이 배양한 결과, Haptophyta의 Prymnesium, Chrysochromulina 및 Phaeocystis 둥의 수 종이 출현하였으며, 일부 종의 경우는 일정기간 지속적으로 배양되어짐을 알 수 있었다.

• Applied Chemistry for Engineering
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• v.21 no.6
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• pp.659-663
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• 2010

Microalgae has been seen all over the seawater and several species are used for human food. Specially, Nannochloropsis oculata, a photosynthetic microalgae, has been focused for a vast array of valuable nutritious compounds. In order to find high mass Nannochloropsis oculata culture conditions, some of important growth factors of pH, temperature, culture media, and $CO_2$ effect were tested. The optimal growth condition was found to be as follows : 3% artificial seawater, initial pH 8.5, and temperature $25^{\circ}C$. The alga mass and chlorophyll content were dramatically increased by applying 5% flue $CO_2$ gas (1.50 g/L algae in a continuous $CO_2$ flue; 0.76 g/L alga without $CO_2$). It was shown that the chlorophyll biosynthesis was also closely associated with alga growth.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.2
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• pp.102-112
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• 2003

This study was carried out to research and develop a shallow green rooftop system which would require low maintenance and therefore could be used for existing rooftops. To achieve these goals, the conceptual model was induced by past studies and the experimental systems were deduced from the conceptual model. On the growth of Sedum sarmentosum grown in these rooftop systems, the effects of artificial substrate type, soil depth, and drainage type were investigated from 3 April to 11 October 2002. Artificial substrates were an alone type and a blending type. The alone type was an artificial substrate formulated by blending crushed porous glass with bark(v/v, 6:4). The blending type was formulated by blending the alone type with loam(v/v, 1:1). Soil depths were 5cm, loom, and 15cm. Drainage types were a reservoir-drainage type and a drainage type. The reservoir-drainage type could keep water and drain excessive water at the same time. The drainage type could drain excessive water but could not keep water. Covering area, total fresh and dry weight, visual quality, and water content per 1g dry matter were measured. All the variables were analyzed by correlation analysis and factor analysis. The results of the study are summarized as follows. The growth increment was higher in the blending type than in the alone type, the highest in loom soil depth and higher in the reservoir-drainage type than in the drainage type. The growth quality was higher in the blending type than in the alone type, the highest in l0cm soil depth, and higher in the drainage type than in the reservoir-drainage type. In consideration of the permissible load on the existing rooftops and the effects of the treatments on the growth increment and quality, the system should adopt the blending type in artificial substrate types, 5~10cm in soil depths, and the drainage type in drainage types. This system will be well-suited to the growth of Sedum sarmentosum, and when the artificial substrate was in field capacity, the weight will be 75~115kg/$m^2$.

• The Korean Journal of Mycology
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• v.11 no.2
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• pp.91-95
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• 1983

To investigate antitumor constituents of Hydnum repandum, which belongs to edible basidiomycetes, the mycelia separated from the carpophore were shake-cultured and subjected to hot water extraction. The extract was concentrated under vacuum and mixed with a three-fold volume of 95% ethanol to yield precipitates. The water soluble fraction of the precipitates was dialyzed and then lyophilized to yield a water soluble protein-polysaccharide fraction (= WPPF). This exerted antitumor activity against sarcoma 180 implanted in ICR mice. When administered i.p. at the dose level of 20mg/kg once daily for ten consecutive days, it showed an inhibition ratio of 54.3%. WPPF was found to be composed of a polysaccharide moiety (42% of WPPF) and a protein moiety (28% of WPPF) when determined by colorimetric method using anthrone reagent and Folin's phenol reagent. The polysaccharide moiety of WPPF was found to contain glucose (57.4%), mannose(19.3%), galactose (10.8%), xylose (6.8%), and fucose (5.7%), when the methanolysate of WPPF was analysed by GLC method.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.4
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• pp.361-367
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• 2015

This paper presents the design concept of an intrinsic continuum robot for safe man-machine interface and characteristic behaviors of its end-effector based on real experiments. Since pneumatic artificial muscles having similar antagonistic actuation to human muscles are used for main backbones of the proposed robot as well as in the role of the actuating devices, variable stiffness of robotic joints can be available in the actual environment. In order to solve the inherent shortcoming of an intrinsic continuum robot due to bending motion of the backbone materials, a Kalman filter scheme based on a triaxial accelerometer and a triaxial gyroscope was proposed to conduct an attitude estimation of the end-effector of the robot. The experimental results verified that the proposed method was effective in estimating the attitude of the end-effector of the intrinsic continuum robot.

• Korean Journal of Agricultural Science
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• v.2 no.2
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• pp.375-380
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• 1975

Effects of light on mycelial growth. pigmentation and sclerotial production of Corticium sasakii were investigated on rice polish agar at $29^{\circ}C=1$. 1) In both isolates. D-3 and D-15. light had no affect on the mycelial growth of C. sasakii. Isolate D-15 did not produce a brown pigment within its mycelium and culture medium in all treatments. but isolate D-3 were more intensively pigmented in light than in darkness. 2) Effect of light on sclerotial production of C. sasakii was different between two isolates. Isolate D-3 produced the most sclerotia in constant light, intermediate in alternating light and darkness and the least in constant darkness. 3) Isolate D-15 produced a little more sclerotia in light than in darkness, but the differences were not statistically significant. However, this isolate produced a larger number of big sclerotia in constant darkness than in constant light and in alternating light and darkness.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.146-152
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• 2005

For the application of ectomycorrhizal seedlings on damaged slope lands, studies on cultural characteristics of Lyophyllum shimeji and ectomycorrhizal associations of Lespedeza cyrtobotrya seedlings were carried out by artificial inoculation of L. shimeji. Mycelial growth of L. shimeji was best on MP (1% malt extract, 0.1% peptone, 1% glucose and 1.5% agar) medium. An optimum temperature and pH for the mycelial growth were $25^{\circ}C$ and pH6, respectively. Mycorrhizal root of L. cyrtobotrya seedlings inoculated with L. shimeji showed characteristics of ectomycorrhizas with Hartig net. Growth rate of the mycorrhizal seedlings's roots was higher than that of non-mycorrhizal seedlings. When the mycorrhizal seedlings were transplanted in slope land, survival rate and dry weight were 62% and 850 mg/seedling, respectively. On the other hand, survival rate and dry weight of non-mycorrhizal seedlings were 11% and 430 mg/seedling, respectively.

• The Korean Journal of Mycology
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• v.12 no.4
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• pp.129-132
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• 1984

To find antitumor constituents in Korean basidiomycetes, the mycelia of Lentinus edodes DMC7, which had shown a good mycelial growth in shakeflasks, were cultured at $27^{\circ}C$ on an orbital shaking incubator at 180 rev/min for 12 days. The medium was composed of glucose (50g/l), yeast extract (9g/l), peptone (9g/l), and seven inorganic salts. A water soluble macromolecular fraction, LF-3, was obtained from the culure filtrate by fractionation with ethanol and dialysis using a Visking tube. When LF-3 was administered i.p. at 50mg/kg/day once daily for 10 consecutive days to female ICR mice which were implanted s.c. with sarcoma 180 $(10^6\;cells/mouse)$, it exerted a highly significant antitumor activity, with the tumor inhibition ratio of 53. 1%.

• Journal of Sericultural and Entomological Science
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• v.45 no.2
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• pp.103-107
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• 2003

Entomopathogenic nematodes were isolated through the investigation in soils collected from cultivated and non-cultivated fields using silkworms (Bombyx mori) and Galleria mellonella trap. The detectable rate of entomopathogenic nematodes of silkworms trap was higher than the G. mellonella trap. This study indicates the detection of entomopathogenic nematodes from soils that silkworms are sensitive superior to the G. mellonella to entomopathogenic nematodes. The steinernema, rhabditidae, and diplogatroidae strains successfully cultured on the silkworms host as well as on artificial media. Reproductivity in the living silkworm larva and pupa was 1.5 to 3.5${\times}$ 10$\^$5/ nematodes per host However, G. mellonella could be multiplied less than 5${\times}$l0$\^$5/ nematodes. The dried pupa of the silkworm following mositurize was cultured 0.5 to 2${\times}$10$\^$5/) nematodes per host. The culture methods of the steinernema, rhabditidae, and diplogatroidae strains, using silkworm powder, extracted chicken intestine, and food waste fertilizer could be applicative, but rate of reproduction was low.