• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.640-652
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• 2009

We have performed mPW1PW91 calculations to investigate the conformational characteristics and hydrogen bonds of p-tert-butylcalix[4]arene (1), p-tert-butylcalix[5]arene (2), calix[6]arene (3) and p-tertbutylcalix[6]arene (4). The structures of the different conformers of 1-3 were optimized by using mPW1PW91/6-31+G(d,p) method. The relative stability of the four conformers of 1 is in the following order: cone (most stable) > partial-cone > 1,2-alternate > 1,3-alternate. The relative stability of the conformers of 2 is in the following order: cone (most stable) > 1,2-alternate > partial-cone > 1,3-alternate. The relative stability of the various conformers of 3 is in the following order: cone (pinched: most stable) > partial-cone > cone (winged) - 1,2-alternate - 1,2,3-alternate > 1,4-alternate > 1,3-alternate > 1,3,5-alternate. The structures of the various conformers of 4 were optimized by using the mPW1PW91/6-31G(d,p) method followed by single point calculation of mPW1PW91/6-31+G(d,p). The relative stability of the conformers of 4 is in the following order: cone (pinched) > 1,2-alternate > cone (winged) > 1,4-alternate - partial-cone > 1,2,3-alternate > 1,3,5-alternate > 1,3-alternate. The primary factor affecting the relative stabilities of the various conformers of the 1-4 are the number and strength of the intramolecular hydrogen bonds. The hydrogen-bond distances are discussed based on two different calculation methods (B3LYP and mPW1PW91).

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.5
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• pp.75-82
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• 2015

An experimental study has been carried out to examine development length effects for high strength headed deformed bars. Current design codes limit the specified yield strength of headed bars to 400 MPa. Such the limit is due to the lack of experimental studies on headed bars made of high strength materials. Thus a test program was planed with headed bars with the yield strength of 600 MPa. The threaded head type with head shapes of round plate and circular cone was selected in this study. The experimental variables were development length, number of bars, and head shape. Specimens were classified into L-type and S-type depending on the development length. The development length of L-type was computed according to the design code without considering the limit. S-type specimens had shorter development lengths than the L-type. Further classification was made depending on the shape of heads. A-types have the head shape of round plate and B-types have the shape of circular cone. Three L-type specimens were fabricated with the variable of number of bars (1, 2, and 3). Four specimens for each of SA and SB types were made with development lengths of 50%, 45%, 40%, and 35% compared with L-type. Pullout tests was carried out with 11 specimens. The test results were compared with computed strengths with the design code equations (Appendix II). Based the current studies, it can be said that high strength headed deformed bars used in this study be able to provide such strengths computed with the current design code without considering the yield strength limit.

• Journal of Life Science
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• v.18 no.10
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• pp.1348-1354
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• 2008

mi transcription factor (MITF) is important in regulating the differentiation of mast cells. In particular, MITF regulates the transcription of the mouse mast cell-specific serine protease (mMCP)-6 gene, which is generally expressed by the connective tissue-type of mast cells. In this study, we investigated alternative isoforms of MITF that regulate transcription of the mMCP-6 gene in bone marrow-derived cultured mast cells in mice. The expression of MITF isoforms was examined by RT-PCR. We observed that MITF-A, -E, -H and -Mc were expressed by mucosal-type mast cells cultured in the presence of IL-3, whereas the connective tissue-type mast cells cultured in the presence of stem cell factor (SCF) expressed MITF-A. Overexpression of MITF isoforms increased luciferase activity through the mMCP-6 promoter in NIH-3T3 cells and elevated the level of mMCP-6 expression in the MC/9 mast cell line. Moreover, mMCP-6 expression in mast cells was significantly inhibited by the depletion of MITF. The transcriptional activity and DNA binding of MITF-A was comparable to that of MITF isoforms, including MITF-E, -H, and -Mc. Our results therefore suggest that MITF-A may be an important isoform of MITF in regulating the transcription of mMCP-6 in mouse connective tissue mast cells.

• Journal of the Korean Chemical Society
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• v.53 no.5
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• pp.521-529
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• 2009

Molecular structures of the various conformers for the 1,3-dimethoxy-p-tert-butylthiacalix[4] crown-5-ether (3) were optimized by using DFT B3LYP/6 - 31 + G(d,p) and mPW1PW91/6 - 31 + G(d,p) (hybrid HF-DF) calculation methods. We have analyzed the energy differences and structures of eight in/out orientations (cone_oo, cone_oi, pc_oo, pc_io, pc_oi, pc_ii, 13a_oo, 13a_io) of two methoxy groups in three major conformations (cone, partial-cone and 1,3-alternate). The 13a_oo (out-out orientation of the 1,3-alternate conformer) is calculated to be the most stable among eight different conformations of 3, and in accord with the experimental result. The ordering of relative stability resulted from the mPW1PW91/6 - 31 + G(d,p) calculation method is following: 13a_oo > 13a_io$\sim$pc_io$\sim$cone_oo > cone_oi$\sim$pc_oo$\sim$pc_oi > pc_ii.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.928-934
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• 2005

ATF2 is a cellular transcription factor which belongs to the CREB/ATF class and it is leucine zipper protein which generally binds to DNA as dimers. This paper presents the procedure for subcloning the ATF2 gene and the results of experiment used the expressed ATF2. The pET expression vector was used since it produced 6xHis fusion protein for easy purification using affinity column. The Nickel chelating chromatography was used for Purifying the expressed ATE2 from E- codi BL2l. Subsequen시y In vitro binding pull-down assay showed the binding specificity of ATF2 with AP-1 family factors such as BATF, c-Fos, c-Jun and ATF2 itselgf. ATF2 forms homodimer as well as strong heterodimer with BATF. It also forms stable dimer with c-Jun but barely binds with c-Fos.

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.1
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• pp.62-71
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• 2015

KCI 2012 and ACI318-11 contains development length provisions for the use of headed deformed bars in tension and does not allow their tension lap splices. In ACI318-11, the confinement factor, such as transverse reinforcement factor, is not used to calculate the development length of headed bars. The purpose of this experimental study is to evaluate the effect of confinement details to the lap splice performance of headed deformed reinforcing bars in grade SD400 and SD500. The confinement details are stirrups and tie-down bars in lap zone. Test results showed that specimens with only stirrups had the brittle failure and could not increase lap strengths, and that specimens with composite confinements by stirrups and tie-down bars had the flexural strengths over than nominal flexural strengths. Stirrups with tie-down bars can have an effect on improvement in lap splice of headed bars in grade SD400 and SD500.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.1093-1096
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• 2008

This study investigated the bond behavior between recycled coarse aggregate (RCA) concrete and deformed reinforcing bars. The position (i.e., vertical, horizontal) and the location (i.e., 375mm, 225mm and 75 mm) of deformed bar were considered as a main test parameter in this paper. From the test results, it was found that maximum bond strength of top reinforcement was decreased compared with that of bottom reinforcement. Also bar embedded horizontally 225mm above from base could not satisfy bond strength requirement provided in CEB-FIP code. It was caused by the fact that bonded area at the bottom of horizontal reinforcement was significantly reduced by the poring water and laitance. In this specimen, the bond strength provided by bearing stress and wedging action of concrete was not fully observed.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.263.2-263
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• 1994

• Electrical & Electronic Materials
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• v.11 no.5
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• pp.394-399
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• 1998

• Electrical & Electronic Materials
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• v.11 no.5
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• pp.412-416
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• 1998