• Title/Summary/Keyword: 이용성 평가

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Characterization of Exolytic GH50A β-Agarase and GH117A α-NABH Involved in Agarose Saccharification of Cellvibrio sp. KY-GH-1 and Possible Application to Mass Production of NA2 and L-AHG (Cellvibrio sp. KY-GH-1의 아가로오스 당화 관련 엑소형 GH50A β-아가레이즈와 GH117A α-NABH의 특성 및 NA2와 L-AHG 양산에의 적용 가능성)

  • Jang, Won Young;Lee, Hee Kyoung;Kim, Young Ho
    • Journal of Life Science
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    • v.31 no.3
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    • pp.356-365
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    • 2021
  • Recently, we sequenced the entire genome of a freshwater agar-degrading bacterium Cellvibrio sp. KY-GH-1 (KCTC13629BP) to explore genetic information encoding agarases that hydrolyze agarose into monomers 3,6-anhydro-L-galactose (L-AHG) and D-galactose. The KY-GH-1 strain appeared to possess nine β-agarase genes and two α-neoagarobiose hydrolase (α-NABH) genes in a 77-kb agarase gene cluster. Based on these genetic information, the KY-GH-1 strain-caused agarose degradation into L-AHG and D-galactose was predicted to be initiated by both endolytic GH16 and GH86 β-agarases to generate NAOS (NA4/NA6/NA8), and further processed by exolytic GH50 β-agarases to generate NA2, and then terminated by GH117 α-NABHs which degrade NA2 into L-AHG and D-galactose. More recently, by employing E. coli expression system with pET-30a vector we obtained three recombinant His-tagged GH50 family β-agarases (GH50A, GH50B, and GH50C) derived from Cellvibrio sp. KY-GH-1 to compare their enzymatic properties. GH50A β-agarase turned out to have the highest exolytic β-agarase activity among the three GH50 isozymes, catalyzing efficient NA2 production from the substrate (agarose, NAOS or AOS). Additionally, we determined that GH117A α-NABH, but not GH117B α-NABH, could potently degrade NA2 into L-AHG and D-galactose. Sequentially, we examined the enzymatic characteristics of GH50A β-agarase and GH117A α-NABH, and assessed their efficiency for NA2 production from agarose and for production of L-AHG and D-galactose from NA2, respectively. In this review, we describe the benefits of recombinant GH50A β-agarase and GH117A α-NABH originated from Cellvibrio sp. KY-GH-1, which may be useful for the enzymatic hydrolysis of agarose for mass production of L-AHG and D-galactose.

Changes in Landscape Characteristics of Stream Habitats with the Construction and Operation of River-Crossing Structures in the Geum-gang River, South Korea (금강에서 횡단구조물의 설치와 운영에 따른 하천 서식처의 경관 특성 변화)

  • Kim, Dana;Lee, Cheolho;Kim, Hwirae;Ock, Giyoung;Cho, Kang-Hyun
    • Ecology and Resilient Infrastructure
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    • v.8 no.1
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    • pp.64-78
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    • 2021
  • This study was conducted to find out the effect of the construction and operation of river-crossing structures on the habitat landscape characteristics in the Geum-gang River, South Korea. A total of three study reaches were selected in the downstream of the Daecheong Dam: the Buyong-ri reach, which is a control that is not affected by the construction and operation of the weir of the Four Rivers Project and Sejong-bo Weir reach and Gongju-bo Weir reach of the upper and lower sections of each weir that are affected by the weir construction and operation. The habitat type was classified, and then the structural characteristics of the landscape were analyzed using aerial photographs taken before and after the construction of the Daecheong Dam, before and after the construction of the weir, and before and after the weir gate operation. After the construction of Daecheong Dam in Geum River, the area of the bare land greatly decreased, and the area of grassland and woodland increased in the downstream of the dam. In addition, the patch number in the river landscape increased, the patch size decreased, and the landscape shape index and the habitat diversity increased. Therefore, after the construction of the dam, the bare land habitat was changed to a vegetated habitat, and the habitat was fragmented and diversified in the downstream of the dam. After the construction of the weirs, the area of open water increased by 18% in the Sejong-bo reach and by 90% in the Gongju-bo reach, and the landscape shape index of the open water decreased by 32% in the Sejong-bo reach and by 35% in the Gongju-bo reach, and the habitat diversity index decreased to 25% in the Sejong-bo reach and to 24% in the Gongju-bo reach. Therefore, the open water habitat was expanded, the shape of the habitat was simplified, and the habitat diversity decreased according to the construction of the weirs. After water-gate opening of the weir, the bare land that disappeared after the construction of the weir reappeared, and the landscape shape index and habitat diversity index increased in both terrestrial and open water habitats. Therefore, it was found that the landscape characteristics of the river habitats were restored to the pre-construction of the weir by the operation of the weir gate. The effect of weir gate opening was delayed in the downstream than in the upstream of the weir. Although the characteristics of the landscape structure in the river habitat changed due to the construction of the river-crossing structures, it is thought that proper technology development for the ecological operation of the structures is necessary as the habitat environments can be restored by the operation of these structures.

The effect of reduced thickness in different regions on the fracture resistance of monolithic zirconia crowns (다양한 부위에서의 감소된 두께가 지르코니아 크라운의 파절 저항에 미치는 영향)

  • Abukabbos, Layla;Park, Je Uk;Lee, Wonsup
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.2
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    • pp.135-142
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    • 2022
  • Purpose. This study aims to evaluate the combined effect of reduced thickness in different regions on the fracture resistance of monolithic zirconia crowns. Materials and methods. Seven nickel-chromium dies were generated from a 3D model of mandibular first molar using the digital scanner with the following geometries: 1.5 mm occlusal reduction, 1.0 mm deep chamfer. Based on the abutment model, Zirconia blocks (Luxen Zirconia) were selected to fabricate Sixty-three zirconia crowns with occlusal thicknesses of 0.3 mm, 0.5 mm, and 1.5 mm, and different axial thicknesses of 0.3 mm, 0.5 mm, and 1.0 mm. All crowns were cemented by resin cement. Next, the crowns were subjected to load-to-fracture test until fracture using an electronic universal testing machine. In addition, fracture patterns were observed with a scanning electron microscope (SEM). Two-way ANOVA and the Tuckey HSD test for post hoc analysis were used for statistical analysis (P < .05). Results. The mean values of fracture resistancerecorded was higher than the average biting force in the posterior region. The two-way ANOVA showed that the occlusal and axial thickness affected the fracture resistance significantly (P < .05). However, the effect of axial thickness on fracture resistance did not show a statistical difference when thicker than 0.5 mm. The observed failure modes were partial or complete fracture depending on the severity of crack propagation. Conclusion. Within the limitations of the present study, the CAD-CAM monolithic zirconia crown with extremely reduced thickness showed adequate fracture resistance to withstand occlusal load in molar regions. In addition, both occlusal and axial thickness affected the fracture resistance of the zirconia crown and showed different results as combined.

Temperature change and performance of bur efficiency for two different drill combinations (두 가지 임플란트 드릴 조합에 따른 온도 변화 및 효율 비교)

  • Hwang-Bo, Heung;Park, Jae-Young;Lee, Sang-Youn;Son, Keunbada;Lee, Kyu-Bok
    • The Journal of Korean Academy of Prosthodontics
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    • v.60 no.2
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    • pp.143-151
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    • 2022
  • Purpose. The purpose of this study was to evaluate the performance efficiency of two different drill combinations according to the heat generated and drilling time. Materials and methods. In this study, cow ribs were used as research materials. To test the specimen, cow bones were rid of fascia and muscles, and a temperature sensor was mounted around the drilling area. The experimental group was divided into a group using a guide drill and a group using a Lindmann drill according to the drill used before the initial drilling. The drilling sequence of the guide drilling group is as follows; guide drill (ø 2.25), initial drill (ø 2.25), twist drill (ø 2.80), and twist drill (ø 3.20). The drilling sequence of the Lindmann drilling group is as follows; Lindmann drill (ø 2.10), initial drill (ø 2.25), twist drill (ø 2.80), and twist drill (ø 3.20). The temperature was measured after drilling. For statistical analysis, the difference between the groups was analyzed using the Mann-Whitney U test and the Friedman test was used (α = .05). Results. The average performance efficiency for each specimen of guide drilling group ranged from 0.3861 to 1.1385 mm3/s and that of Lindmann drilling group ranged from 0.1700 to 0.4199 mm3/s. The two drill combinations contained a guide drill and Lindmann drill as their first drills. The combination using the guide drill demonstrated excellent performance efficiency when calculated using the drilling time (P < .001). Conclusion. Since the guide drill group showed better performance efficiency than the Lindmann drill group, the use of the guide drill was more suitable for the primary drilling process.

Changes in Meteorological Variables by SO2 Emissions over East Asia using a Linux-based U.K. Earth System Model (리눅스 기반 U.K. 지구시스템모형을 이용한 동아시아 SO2 배출에 따른 기상장 변화)

  • Youn, Daeok;Song, Hyunggyu;Lee, Johan
    • Journal of the Korean earth science society
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    • v.43 no.1
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    • pp.60-76
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    • 2022
  • This study presents a software full setup and the following test execution times in a Linux cluster for the United Kingdom Earth System Model (UKESM) and then compares the model results from control and experimental simulations of the UKESM relative to various observations. Despite its low resolution, the latest version of the UKESM can simulate tropospheric chemistry-aerosol processes and the stratospheric ozone chemistry using the United Kingdom Chemistry and Aerosol (UKCA) module. The UKESM with UKCA (UKESM-UKCA) can treat atmospheric chemistryaerosol-cloud-radiation interactions throughout the whole atmosphere. In addition to the control UKESM run with the default CMIP5 SO2 emission dataset, an experimental run was conducted to evaluate the aerosol effects on meteorology by changing atmospheric SO2 loading with the newest REAS data over East Asia. The simulation period of the two model runs was 28 years, from January 1, 1982 to December 31, 2009. Spatial distributions of monthly mean aerosol optical depth, 2-m temperature, and precipitation intensity from model simulations and observations over East Asia were compared. The spatial patterns of surface temperature and precipitation from the two model simulations were generally in reasonable agreement with the observations. The simulated ozone concentration and total column ozone also agreed reasonably with the ERA5 reanalyzed one. Comparisons of spatial patterns and linear trends led to the conclusion that the model simulation with the newest SO2 emission dataset over East Asia showed better temporal changes in temperature and precipitation over the western Pacific and inland China. Our results are in line with previous finding that SO2 emissions over East Asia are an important factor for the atmospheric environment and climate change. This study confirms that the UKESM can be installed and operated in a Linux cluster-computing environment. Thus, researchers in various fields would have better access to the UKESM, which can handle the carbon cycle and atmospheric environment on Earth with interactions between the atmosphere, ocean, sea ice, and land.

Development and Performance Evaluation of Multi-sensor Module for Use in Disaster Sites of Mobile Robot (조사로봇의 재난현장 활용을 위한 다중센서모듈 개발 및 성능평가에 관한 연구)

  • Jung, Yonghan;Hong, Junwooh;Han, Soohee;Shin, Dongyoon;Lim, Eontaek;Kim, Seongsam
    • Korean Journal of Remote Sensing
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    • v.38 no.6_3
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    • pp.1827-1836
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    • 2022
  • Disasters that occur unexpectedly are difficult to predict. In addition, the scale and damage are increasing compared to the past. Sometimes one disaster can develop into another disaster. Among the four stages of disaster management, search and rescue are carried out in the response stage when an emergency occurs. Therefore, personnel such as firefighters who are put into the scene are put in at a lot of risk. In this respect, in the initial response process at the disaster site, robots are a technology with high potential to reduce damage to human life and property. In addition, Light Detection And Ranging (LiDAR) can acquire a relatively wide range of 3D information using a laser. Due to its high accuracy and precision, it is a very useful sensor when considering the characteristics of a disaster site. Therefore, in this study, development and experiments were conducted so that the robot could perform real-time monitoring at the disaster site. Multi-sensor module was developed by combining LiDAR, Inertial Measurement Unit (IMU) sensor, and computing board. Then, this module was mounted on the robot, and a customized Simultaneous Localization and Mapping (SLAM) algorithm was developed. A method for stably mounting a multi-sensor module to a robot to maintain optimal accuracy at disaster sites was studied. And to check the performance of the module, SLAM was tested inside the disaster building, and various SLAM algorithms and distance comparisons were performed. As a result, PackSLAM developed in this study showed lower error compared to other algorithms, showing the possibility of application in disaster sites. In the future, in order to further enhance usability at disaster sites, various experiments will be conducted by establishing a rough terrain environment with many obstacles.

Anterolateral Ligament of the Knee: Anatomy, Biomechanics, Techniques, and Clinical Outcome (슬관절 전외측인대의 해부학, 생역학, 수술법 및 임상적 결과)

  • Kim, Seong Hwan;Lee, Tae-Hyub;Park, Yong-Beom
    • Journal of the Korean Orthopaedic Association
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    • v.55 no.4
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    • pp.281-293
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    • 2020
  • An anterior cruciate ligament (ACL) reconstruction is one of the most frequent surgical procedures in the knee joint, but despite the better understanding of anatomy and biomechanics, surgical reconstruction procedures still fail to restore rotational stability in 7%-16% of patients. Hence, many studies have attempted to identify the factors for rotational laxity, including the anterolateral ligament (ALL), but still showed controversies. Descriptions of the ALL anatomy are also confused by overlapping nomenclature, but it is usually known as a distinctive fiber running in an anteroinferior and oblique direction from the lateral epicondyle of the femur to the proximal anterolateral tibia, between the fibular head and Gerdy's tubercle. The importance of the ALL as a secondary restraint in the knee has been emphasized for successful ACL reconstructions that can restore rotational stability, but there is still some controversy. Some studies reported that the ALL could be a restraint to the tibial rotation, but not to anterior tibial translation. On the other hand, some studies reported that the role of ALL in rotational stability would be limited as a secondary structure because it bears loads only beyond normal biomechanical motion. The diagnosis of an ALL injury can be performed by a physical examination, radiology examination, and magnetic resonance imaging, but it should be assessed using a multimodal approach. Recently, ALL was considered one of the anterolateral complex structures, as well as the Kaplan fiber in the iliotibial band. Many studies have introduced many indications and treatment options, but there is still some debate. The treatment methods are introduced mainly as ALL reconstructions or lateral extra-articular tenodesis, which can achieve additional benefit to the knee stability. Further studies will be needed on the indications and proper surgical methods of ALL treatment.

Initial Analysis of the Underground Air Among Jeju Lava Forest(Sumgol) and its Healing Effect on the Human Body (제주 현무암 '숲' 지하 공기(숨골: Sumgol)의 분석과 인체에 미치는 치유 효과)

  • Sin, SBangsik;Kim, Hyek Nyeon;Lee, Deok Hee;Kim, Tae Seung;Kim, Yong Hwan;Kang, Chang Hee;Song, Kyu Jin;Lee, Hyung H.
    • Journal of Naturopathy
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    • v.11 no.1
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    • pp.18-30
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    • 2022
  • Background: It was to develop an air purification system (APS) using an underground air purification layer to verify the effect of basalt forest's underground air (sumgol) on a volcanic Jeju. Finally, it is necessary to analyze these purified air components and their usefulness to the human body in an air experience center. Purpose: It was to collect basalt forest air, analyze its composition, and explore its effect on the human body. Methods: We APS devices installed at four points in the Papaville area of Jeju. The air discharged from the APS was collected and analyzed the recycling components. An installed experience room filled with negative ions is about 5,000 ions/m3. After allowing the participants to stay for 60 to 120 minutes, we investigated the state of blood vessels. Results: In the analysis of the underground air, the O2 concentration was 21.18%, which was higher than the average oxygen concentration of 20.94% in the atmosphere. However, Formaldehyde was not detected, and the CO2 was 419 ppm, which was lower than that of indoor air. The PM2.5 concentration was less than 24 ㎍/m3 and detected anions over 5.000 /m3. The experiencer's vascular states improved, and the increase in pulse rate and stress relief were high. Conclusions: The valuable ingredients identified by analyzing the air were precious for natural healing. The experience results showed that it effectively improved the pulse rate, blood vessels, and stress. These conditions may be highly beneficial as a new area for expanding the basalt lava forest in the Jeju area into the natural healing and wellness industry.

The Types of Warm Temperate Forest and the Degraded Levels in the Island Area of the West and South Coast (서남해안 도서지역의 난온대 식생유형화 및 훼손등급)

  • Park, Seok-Gon;Sung, Chan-Yong;Kang, Hyun-Mi
    • Korean Journal of Environment and Ecology
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    • v.35 no.6
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    • pp.579-593
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    • 2021
  • In order to understand the types of vegetation in warm temperate-climate zones, vegetation was investigated in several island areas in Jeollanam-do (Jindo, Wando, Gangjin, Goheung, and Yeosu). The evaluation standard for degraded level of warm temperate forests were proposed based on the importance percentage (IP) in canopy layer of the evergreen broad-leaf forests and the number of arboreal evergreen broad-leaf species. Through these measurements, the restoration types and techniques for each degraded level were estimated, and it is intended to be used in establishing restoration plans for the southwest coast island area. The vegetation was analyzed using the two-way indicator species analysis (TWINSPAN) method using survey data of 307 plots. As a result, it was divided into 8 communities, and the appearance characteristics of evergreen broad-leaf species were identified in each community. Community I was located on the lower slope at an altitude of 86.6 m, and Neolitsea sericea and Castanopsis sieboldii were dominant. Communities II and III were the vegetation types that appear on the coast below an altitude of 10.5 to 22.5 m, and Machilus thunbergii, Cinnamomum japonicum, N. sericea, and C. sieboldii were dominant. Communities IV and V were vegetation types that appeared in the lower and middle slops between the altitudes of 71.9 to 153.4m, and C. sieboldii was dominant. In community VI, the N. sericea was dominant in the lower and middle slops at an altitude of 166.9 m. The last communities VII and VIII were the vegetation types that appeared on the middle slop at an altitude of 187.8 to 246.2 m. Also, Quercus acuta and Q. salicina were present. In summary, the evergreen broad-leaf forests dominated by M. thunbergii, C. japonicum, and N. sericea appeared mainly in the coastal areas of the lowlands. The community of C. sieboldii was distributed higher inland than this community. The communities that appeared mainly in the inland highlands at levels above these two communities were Q. acuta and Q. salicina. The degraded level was classified as 0 to V, according to the IP of arboreal evergreen broad-leaf species and the number of arboreal evergreen broad-leaf species. According to the degraded level, the restoration types (preservation, induction, improvement, creation) and the restoration techniques were determined.

Grapevine Growth and Berry Development under the Agrivoltaic Solar Panels in the Vineyards (영농형 태양광 시설 설치에 따른 포도나무 생육 및 과실 특성 변화 비교)

  • Ahn, Soon Young;Lee, Dan Bi;Lee, Hae In;Myint, Zar Le;Min, Sang Yoon;Kim, Bo Myung;Oh, Wook;Jung, Jae Hak;Yun, Hae Keun
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.356-365
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    • 2022
  • Agrivoltaic systems, also called solar sharing, stated from an idea that utilizes sunlight above the light saturation point of crops for power generation using solar panels. The agrivoltaic systems are expected to reduce the incident solar radiation, the consequent surface cooling effect, and evapotranspiration, and bring additional income to farms through solar power generation by combining crops with solar photovoltaics. In this study, to evaluate if agrivoltaic systems are suitable for viticulture, we investigated the microclimatic change, the growth of vines and the characteristics of grape grown under solar panels set by planting lines compared with ones in open vineyards. There was high reduction of wind speed during over-wintering season, and low soil temperature under solar panel compared to those in the open field. There was not significant difference in total carbohydrates and bud burst in bearing mother branches between plots. Despite high content of chlorophyll in vines grown under panels, there is no significant difference in shoot growth of vines, berry weight, cluster weight, total soluble solid content and acidity of berries, and anthocyanin content of berry skins in harvested grapes in vineyards under panels and open vineyards. It was observed that harvesting season was delayed by 7-10 days due to late skin coloration in grapes grown in vineyards under panels compared to ones grown in open vineyards. The results from this study would be used as data required in development of viticulture system under panel in the future and further study for evaluating the influence of agrivoltaic system on production of crops including grapes.