• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1975.12a
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• pp.180.3-180
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• 1975

당질로서 xylose 및 xylan을 기질로 하는 영양 배치에서 강력한 포도당 이성화 효소를 유도 생산하는 Streptomyces속 군주를 약 90종의 토양분리균에서 분리하였다. 이들 중에서 가장 강한 이성화 효소를 나타내는 6종의 Streptcmyces sp.를 분리하여 다음의 결과를 얻었다.(중략)

• KSBB Journal
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• v.10 no.1
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• pp.9-14
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• 1995

Continuous production of fructooligosaccharides from sucrose by a dual immobilized enzyme system of fructosyltransferase and glucose isomerase was studied in a column reactor. The optimal temperature and pH of the immobilized fructosyltransferase were $65^{\circ}C$ and 5.5, respectively. The activity of glucose isomerase was favorable as temperature and pH were increased within the ranges examined. However, both the immobilized enzymes were thermally unstable over $5^{\circ}C$, suggesting that long-term operation of the dual immobilized enzyme column should be carried out below $50^{\circ}C$. The optimum packing ratio of fructosyltransferase to glucose isomerase was found to be around 5/3. Under the optimized reaction conditions, the dual enzyme column was successfully operated for 40 days without any loss of initial enzyme activities, yielding 66% of fructooligosaccharides. Furthermore, the relative sweetness of fructooligosaccharides produced by a dual emzyme system was enhanced by 6% compared with that of fructosyltransferase alone.

• Food Industry And Nutrition
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• v.2 no.1
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• pp.7-9
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• 1997

내열설 미생물, Thermus caldophilus CK24에 대한 탄수화물 생합성을 연구하는 과정에서 다양한 탄수화물 관련효소를 탐색하고 그레 대한 생화학적 및 분자생물학적 연구를 수행하고 있다. 일차로 내열성 미생물내 1) 당핵산염 합성효소와 당전이 효소, 2) 탄수화물 대사효소. 3)탄수화물 분해 및 전환효소의 존재를 HPLC/Bio-LC분석을 통하여 확인하고 이들에 대한 연구를 진행하고 있다. 본 연구발표에서는 포도당을 과당으로 전환하는 이성화효소(xylose isomerase), 그리고 맥아당을 트레할로스로 전환하는 트레할로스 합성효소(trehalose synthase)를 소개하고저 한다. 이성화효소는 이미 산업적 과당 생산에서 대규모적으로 사용되고 있는 식품산업효소이다. 본 연구에서는 Thermus caldophilus GK24, Thermus thermophilus HB8, Thermus flavus AT62 3종의 내열성 미생물에 대한 이성화효소 유전자를 클로닝 하고, 각 재조합하고 이성화효소를 대량생산하였다. 이 내열성 이성화효소는 최적 반응 온도가 8$0^{\circ}C$이고, 포도당을 과당으로 전환하는 수유른 55%이었다. 이러한 과당전환률은 이미 산업적으로 사용되고 있는 이성화효소의 과당전환률(43%)보다 훨씬 높은 것으로 과당 생산공정의 단순화의 생산성 향상에 결정적인 요인이라 할 수 있다. 한편 본 이성화효소의 산업적 특성을 증대하기 위하여 구조-기능관계 연구를 착수하였다. 우선 내열성 이산화 효소의 입체 구조를 결정하였고, 구조조정에 따른 기능적 특성을 조사하기 위하여 특정 위치의 선택적 변이 연구를 진행하고 있다. 끝으로 포도당 전이 효소를 추적하던 과정에서 맥아당을 트레할로스로 전환하는 새로운 효소를 Thermus caldo-philus GK24에서 발견하였다. 그 트레할로스 합성효소는 분자량이 약 110kDa이고 최적 반응온도가 75$^{\circ}C$이면, 조효소없이 맥아당을 트레할로스로 80%이상 전환해 주는 가역효소이었다. 본 연구에서는 효소반응의 조건과 특성을 조사하였고, 효소 아미노-밀단의 서열결정정보를 통하여 효소의 유전자를 클로닝 하고 그 유전자의 구조와 발현연구를 진행하고 있다.

• Korean Journal of Food Science and Technology
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• v.11 no.3
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• pp.192-199
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• 1979

With cells of Streptomyces spp K-45 isolated from soil, the immobilization of glucose isomerase by a series of treatments ; heat, carefully manipulated drying, extrusion with a thickening agent, and glutaraldehyde-induced crosslinking, was presented. This was aimed to obtain a mechanically stable form of whole cell containing glucose isomerase. The resulted pellet form had a good mechanical strength, compared with a commercial product, and showed 26 % of the activity recovery. The specific activity was 48.1 units per g of the dry material. The immobilized glucose isomerase generally showed properties similar to those of the soluble enzyme ; optimal pH at $7.5{\sim}9.0$, optimal temperature at $80{\sim}85^{\circ}C$, activation energy of 10.9 kcal/mole, and $K_m$ for glucose of 10.9M. The immobilized enzyme was very thermostable and pH stable.

• Journal of the Korean Society of Propulsion Engineers
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• v.16 no.2
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• pp.17-24
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• 2012

Tetrahydrotricyclopentadiene(below THTCPD) isomer is a good candidate materials for the high performance liquid fuel component because of its high density and heat of combustion value. The object of this study was to find out the proper reaction condition to improve the fluidity of THTCPD which is solid state at room temperature. Therefore, we have carried out isomerization reactions using aluminum chloride in the varying reaction condition such as reaction temperature and solvents. The results showed that when using aluminum chloride catalyst, THTCPD isomerization reaction was more active in the polar halogenated reaction media such as dichloromethane(methylene chloride: MC), 1,2-dichloroethane(ethylene chloride: EC) and chloroform than in non-polar hydrocarbon media such as n-Hexnae and toluene and was effected by reaction temperature variation.

• Korean Journal of Food Science and Technology
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• v.10 no.4
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• pp.380-386
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• 1978

Enzymatic characteristics of glucose isomerase from Streptomyces spp. K-14 were studied. The optimum pH and temperature of the enzyme reaction are $pH\;7.5{\sim}8.0$ and $70^{\circ}{\sim}75^{\circ}C$, respectively, in the presence of 5 mM $MgSO_4{\cdot}7H_2O$ and 2 mM $CoCl_2{\cdot}6H_2O$. The enzyme activity was activated by both $Mg^{++}$ and $Co.^{++}\;Mg^{++}$ is required for the initial activation of the isomerization reaction, whereas $Co^{++}$ was essential for the increased stability of the enzyme protein. Glucose concentration up to 60% did not affect the reaction velocity as well as the equilibrium conversion of the enzyme.

• Korean Chemical Engineering Research
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• v.46 no.4
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• pp.692-696
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• 2008

There is not much method of using C4 Raffinate III, despite having high olefin contents. The majority of the C4 Raffinate III have been converted into n-butane through hydrogenation, and sold as LPG. The C4 Raffinate III is rich 2-butenes with very low isobutene and isobutene contents. The 2-butenes are converted into 1-butene in the vicinity of thermodynamic equilibrium yield through positional isomerization with n-almumina catalyst calcinated at $400{\sim}600^{\circ}C$. The overall process is composed of isomerization-reactor, de-1-buteneizer to prepare the reactants and to enrich reactive products, and 1-butene column to product a high purity 1-butene. The production of 1-butene increases by 40~60 wt% with the selective positional isomerization from the existing separation method.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.11a
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• pp.800-803
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• 2010

Tetrahydrotricyclopentadiene(below THTCPD) isomer is a good candidate for the high performance liquid fuel component because of high density and heat of combustion value. But it has bad fluidity. The object of this study was to find out the optimal reaction condition to improve the fluidity of THTCPD because initial reactant is solid state, So we have carried out isomerization reactions using aluminum chloride catalyst under varying the reaction condition such as reaction temperature and reaction solvents. The results showed that when using aluminum chloride catalyst, isomerization reaction was more active in dichloromethane(methylene chloride: MC), 1,2-dichloroethane(ethylene chloride: EC) and chloroform than n-hexnae and toluene and was effected by reaction temperature.

• Korean Journal of Food Science and Technology
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• v.11 no.4
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• pp.249-257
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• 1979

Using the whole cell immobilized glucose isomerase which was prepared in the previous work (Korean J. Food Sci. & Technol., 11(3), 192 (1979), the specific activity of the immobilized enzyme was 48.1 units in the batch reaction system and 114 units in the continuous reaction system per g of matrix, respectively. In the continuous reactor the voidity was 0.36, which was suitable for the packed bed reactor. This immobilized enzyme showed a good operational stability of 115 days of half life which was sufficient for the continuous operation. The experimental result showed that 55 % of the substrate was converted to the product in the packed bed reactor. The productivity was dependent on the flow rate, column geometry, enzyme loading, and substrate concentration. An intrapaticle diffusion was observed by the effectiveness factor of 0.75 and interparticle diffusion by the decrease of Km' with increasing the superficial velocity.

• Applied Biological Chemistry
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• v.26 no.4
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• pp.222-230
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• 1983

This study was designed to develop a process for the immobilization of xylose isomerase(D-xylose ketol isomerase, EC 5.3.1.5) from Streptomyces griseolus previously isolated by the authors and its application on a pilot plant scale for the production of high fructose corn syrup. The biomass which has endo-excreted xylose isomerase was homogenized under a pressure of $500kg/cm^2$ and 90.8% of the enzyme recovery of the native activity was obtained as compared to 54.7% recovery by the lysozyme treatment. Ionic bonding method was adopted for the enzyme immobilization due to its many reported merits. It was found that the porous resins such as Diaion HP 20, Duolite A-7, Amberlite IRA 93 and 94 were effective in immobilizing the enzyme. In addition, it was disclosed that the regeneration form of $BO_4--$ is effective for Amberlite IRA 93 and $HCO_3-$ for Diaion HP 20. Optimal immobilization condition for Amberlite IRA 93 was pH 8.0 and $55^{\circ}C$ yielding 80.6% of immobilization. Activity decay test showed half life of the immobilized enzyme with Amberlite IRA 93 was more than 24 days at $65^{\circ}C$. The carrier was evaluated to be resuable and its result showed the relative immobilization yields were 98.2, 93.3, 90.7 and 87.5%, respectively at second, third, forth and fifth rebinding test of the enzyme on Amberlite IRA 93. Optimal temperature of the immobilized enzyme was slightly lowered and the range widened to $60\sim70^{\circ}C$, while optimal pH moved toward $8.0\sim8.3$ in its isomerization reaction. The trial production result of high fructose corn syrup in pilot scale immobilization showed that one liter of immobilized xylose isomerase (350 IXIU/ml-R) is capable producing about 293l high fructose corn syrup(75% dry substance) in 30 days.