• Journal of Korean Society of Forest Science
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• v.113 no.1
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• pp.97-106
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• 2024

Evaluating carbon (C) and nitrogen (N) inputs from litterfall is important for soil nutrient management to enhance forest productivity and to understand the mechanisms of nutrient cycling in forest ecosystems. This study was conducted to compare C and N inputs from litterfall components of Cryptomeria japonica D. Don an d Chamaecyparis obtusa Endlicher planted in adjacent sites in the Jinju Research and Experimental Forests in Gyeongsangnam-do, South Korea. Litterfall into litter traps was collected at three-month intervals between December 2020 and December 2021, and the C and N concentrations of the litterfall components were measured. Litterfall amounts were not significantly different between the plantations, except for reproductive litterfall components. Litterfall accumulation peaked between December and March. The litterfall C concentration in the needle and seed litterfall was significantly higher for C. obtusa than for C. japonica. By contrast, the C concentrations in needle and flower litterfall differed seasonally. The mean N concentration of needle litterfall was significantly higher in C. japonica (0.96%) and C. obtusa collected between June and September (1.01%) than in the other seasons (C. japonica: 0.43%; C. obtusa: 0.53%). Carbon and N inputs in both plantations were highest in needle litterfall collected from December to March and lowest in needle litterfall collected from June to September. Annual C input by litterfall was similar between the plantations (C. japonica: 3,054 kg C ha^-1 yr^-1; C. obtusa: 3,129 kg C ha^-1 yr^-1), whereas total N input was higher for C. japonica (46.93 kg N ha^-1 yr^-1) than for C. obtusa (25.17 kg N ha^-1 yr^-1). The higher N input in the C. japonica plantation than in the C. obtusa plantation was associated with the input of reproductive components. These results could be applied to improve stand-scale models of C and N cycling by litterfall components in C. japonica an d C. obtusa plantations.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.3
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• pp.21-29
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• 2023

In this study, the stress characteristics of temporary fixed steel rods were analyzed in the "temporary fixing system using internal prestressing tension", which is mainly applied to the construction of superstructures by FCM. It was difficult to confirm the changes in initial tensile force in this system because the steel rod was internally connected to the pier and the PSC BOX. Therefore, measurement was performed before and after the completion of each segment using an FBG sensor to measure the change in the micro length of the steel rod. The results of the analysis showed that 75% to 90% of the maximum vertical contraction of the steel rod that occurred until the completion of the cantilever segment occurred in the fixing ~ 1segment, and the maximum loss of initial prestressing force was 39%. Such excessive loss of tension force to 1 segment means that tension is needed to improve the precision of construction during the fixation, and re-tension is needed to secure stability for conduction of cantilever segments after the completion of 1segment. In the 2 ~ last segment, the stress of the steel rod decreased gradually, and in the summer, the decrease in stress tended to partially recover due to the increase in the length of the steel rod corresponding to the increase in the vertical volume of PSC BOX. The dominant factor in the stress change in 2~ last segment in this phenomenon is judged to be the change in the length of the steel rod according to the temperature. Unlike the change in length, the relaxation was 1.2-2.7%, which was mostly offset by the opposite stress corresponding to the temperature stress. Therefore, a plan was proposed to improve the internal stress, such as adjusting the fixation time.

• Journal of Life Science
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• v.34 no.2
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• pp.86-93
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• 2024

Since replication of plasmids must be strictly controlled, plasmids that generally perform rolling circle replication generally maintain a constant copy number by strictly controlling the replication initiator Rep at the transcriptional and translational levels. Plasmid pJB01 contains three orfs (copA, repB, repC or repABC) consisting of a single operon. From analysis of amino acid sequence, pJB01 CopA was homologous to the Cops, as a copy number control protein, of other plasmids. When compared with a CopG of pMV158, CopA seems to form the RHH (ribbon-helix-helix) known as a motif of generalized repressor of plasmids. The result of gel mobility shift assay (EMSA) revealed that the purified fusion CopA protein binds to the operator region of the repABC operon. To examine the functional role of CopA on transcriptional level, 3 point mutants were constructed in coding frame of copA such as CopA R16M, K26R and E50V. The repABC mRNA levels of CopA R16M, K26R and E50V mutants increased 1.84, 1.78 and 2.86 folds more than that of CopA wt, respectively. Furthermore, copy numbers owing to mutations in three copA genes also increased 1.86, 1.68 and 2.89 folds more than that of copA wt, respectively. These results suggest that CopA is the transcriptional repressor, and lowers the copy number of pJB01 by reducing repABC mRNA and then RepB, as a replication initiator.