• Journal of Genetic Medicine
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• v.6 no.2
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• pp.146-154
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• 2009

Multiplex ligation dependent probe amplification (MLPA) is a PCR-based method to detect gene dosage. Since its introduction, MLPA has been used to test a large number of genes for major deletions or duplications. Genetic testing, as a diagnostic tool for genetic disease, has been used primarily to identify point mutations, including base substitutions and small insertions/deletions, using PCR and sequence analysis. However, it is difficult to identify large deletions or duplications using routine PCR- gel based assays, especially in heterozygotes. The MLPA is a more feasible method for identification of gene dosage than another routine PCR-based methods, and better able to detect deleterious deletions or duplications. In addition to detection of gene dosage, MLPA can be applied to identify methylation patterns of target genes, aneuploidy during prenatal diagnoses, and large deletions or duplications that may be associated with various cancers. The MLPA method offers numerous advantages, as it requires only a small amount of template DNA, is applicable to a wide variety of applications, and is high-throughput. On the other hand, this method suffers from disadvantages including the possibility of false positive results affected by template DNA quality, difficulties identifying SNPs located in probe sequences, and analytical complications in quantitative aspects.

• Journal of Korean Society of Steel Construction
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• v.18 no.5
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• pp.543-551
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• 2006

Cold-formed steel studs that are being used as load-bearing members of wall panels for steel houses have a problem with their insulation due to the heat bridging of their web. Some additional thermal insulating materials should be used. To solve this problem, the new-concept hybrid stud, which consists of a galvanized steel sheet (t = 1.0 m - 12.0 m) and a GFRP panel (t = 4.0-6.0 mm), has recently been developed. An investigation on the structural behavior and the strength capacity of this new hybrid stud has been conducted so that it can be used in load-bearing wall panels of residential buildings. This paper describes the axial compression-torsion test results of the hybrid studs under both axial compression and torsion using ATTM. The main factors of the test were the stud length, the magnitude of the initial compressive force, and the loading method of the monotonic or cyclic loading. The torsion was applied increasingly while the initial compression was kept constant to the failure of the hybrid section. The advanced analysis results obtained form the finite element procedure that considered the material properties of the high-strength galvanized steel and the GFRP were compared with the test results for verification.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5906-5914
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• 2013

Recently in Korea, the policy is being proceeded to build a intergenerational housing on artificial ground of railroad site for utilizing rental house. Due to narrow space of rail road site, suitable method have to be developed such as micropiles which is known as a method of a fast construction. However, If micropile is used as foundations for the super structure, construction cost is increases compared with other pile. Consequently, new concept micropile proposed to improve both bearing capacity and cost efficiency of general micropile. New concept micropile consists of waveform cement grout surrounding tread bar that formed by grouting the soil layer with jet grouting method as control the grout pressure and flow. The micropile with waveform is expected to decrease the construction cost by cut down pile length of general micropile. This paper examined the behavior of the new concept micropile with waveform subjected to axial load using two-dimensional axisymmetric numerical analyses method. According to the numerical result, there will cost effectiveness as the pile displacement decreased despite the length of waveform micropile is down about 5% from a general micropile under the same loading condition. Also, the effect of skin friction force which mobilized from the waveform of micropile appeared at relatively soft ground.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.11
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• pp.511-514
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• 1997

The objective of this study is to propose a finite element based design of the dental implant replacing unction and shape of natural teeth. For this, geometric actors were varied to investigate stress distribution of the alveolar bone around dental implant. In this study, the results were obtained based on the theory of linear elastic, with geometrically axisymmetric assumption. Geometric actors determining implant shape are ranged as 0.2mm-0.6mm, 0.04mm-0.1mm, 0.46mm-0.84mm or height of thread, radius of curvature of thread, and pitch, respectively. The stresses in the alveolar bone caused by biting force playa major role in determining implant stability. Especially, the stress concentration in the cortical bone causes bone resorption and finally makes the implant unstable. Therefore, the stress distributions were investigated on the side of the alveolar bone focusing on the area of cortical bone. The maximum von Mises stress was found to increase up to 6% as the height of thread increases, while its value was to decrease to 19% when the radius of curvature increase within the assigned ranges. For the variation of pitch, the larger size of pitch results in greater maximum von Mises stress when the length of the implant under consideration is fixed. The existence of the neck below the shoulder did not affect the stress distribution in the region of alveolar bone. However, the stresses on the side of the implant near the neck were found to be different by 20% approximately. Therefore, the neck can provide the stability of the implant against continuing biting movement. As a conclusion, the finite element based study shows a potential in designing the dental implant systematically.

• Journal of Korean Society of Steel Construction
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• v.9 no.4 s.33
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• pp.659-672
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• 1997

Thermal response induced from nonlinear temperature distribution in composite box gilder bridges depends on several variables(environmental conditions, physical and material properties, location and orientation of bridge, and cross-section geometry). In this paper, parametric study are conducted in order to find the effects of variations of seasons, location and orientation of bridge, sectional geometry and some material properties on the axial deformation, curvature and stresses in composite box girder bridge. A two-dimensional transient finite element model to conduct this parametric studies is briefly presented. Firstly, the effects of the parameters on the diurnal variation of curvature are considered, and for the time of maximum curvature, on the distribution of temperature and stresses of composite box girder sectional are considered. Finally, some considerations about the influence of the parameters on the daily maximum values of axial deformation, curvature and stresses are carried out. The influence of thermal effect on structures is important as much as the influence of live or dead load in some cases. In the design of steel composite bridges, the thermal stresses calculated on the supposition that the temperature difference between the concrete slab and steel girder is $10^{\circ}C$ and the temperature distributions are uniform in concrete slab and steel girder can be underestimated.

• Journal of Korean Society of Steel Construction
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• v.23 no.6
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• pp.747-761
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• 2011

This paper presents an investigation on the change in the statical behavior and the ultimate capacity of steel cable-stayed bridges after cable failure. Cable failure can occur due to fire, direct vehicle clash accidents, cable or anchorage fatigue, and so on. Moreover, the cable may be temporarily disconnected during cable replacement work. When cable failure occurs, the load, that was supported by the broken cable is first transferred to another cable. Then the structural state changes due to the interaction between the girder, mast, and cables. Moreover, it can be predicted that the ultimate capacity will decrease after cable failure, because of the loss of the support system. In this study, the analysis method is suggested to find the new equilibrium state after cable failure based on the theory of nonlinear finite element analysis. Moreover, the ultimate analysis method is also suggested to analyze the ultimate behavior of live loads after cable failure. For a more rational analysis, a three-step analysis procedure is suggested and used, which consisted of initial shape analysis, cable failure analysis, and live load analysis. Using this analysis method, an analytical study was performed to investigate the changes in the structural state and ultimate behavior of steel cable-stayed bridges.

• Journal of Korean Society of Steel Construction
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• v.21 no.6
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• pp.585-596
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• 2009

In the construction of end-plate connections, the end-plate is welded to the end of the beam in a factory and fastened by bolts in the field. This connection is widely used in advanced countries such as European countries and the U.S. Its design and connection details are prescribed in Eurocode 3, AISC LRFD, and FEMA 350. In Korea, the standards for seismic design in KBC 2005 have been reinforced based on IBC 2000 in the U.S., and it is expected that the connection details in the U.S. will be adopted for the establishment of beam-to-column design standards. In the U.S. thick end-plates are used for the connections to prevent beam rupture. The use of the connections in Korea, however, may lead toover-design. In this experimental study, the design standards for the end-plate connections provided by FEMA-350 were analyzed and structural tests for six specimens were conducted with the variables being the shim plate and the connection shape, to provide the best specifications for connections with plastic deformation in the end-plate for use in Korea.

• Journal of Korean Society of Steel Construction
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• v.26 no.4
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• pp.323-334
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• 2014

In this paper, experimental program and associated numerical study were carried out to evaluate the fire resistance of unprotected concrete-filled rectangular steel tubular (CFT) columns subjected to the standard fire. The key testing parameters included the length effect, the load ratio, and the sectional dimensions of the CFT columns. Temperature distribution and axial deformation of the CFT column specimens were measured and analyzed. Rather early local buckling of steel tubes was observed in all the specimens. This caused subsequent load transfer from steel tube to concrete, and eventually triggered concrete crushing, or complete loss of the load bearing capacity of the column. This implies that the limit state of local buckling as well as overall flexural buckling should be incorporated in fire design procedure. As expected, the fire resistance time of specimen with higher load ratio consistently lessened. The prediction of fire resistance time of unprotected CFT columns based on the limiting steel temperature in current design codes or the formula proposed by previous studies is slightly conservative compared to the fire test results available. To establish the finite element analysis model that can be used to predict the thermal and structural behaviour of unprotected CFT columns in fire, the fully coupled thermal-stress analysis was also tried by using the commercial code ABAQUS. The numerical results showed a reasonable global correlation with the experimental results.

• Journal of the Korea institute for structural maintenance and inspection
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• v.17 no.6
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• pp.21-30
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• 2013

As increasing number of large-size earthquake, the social interest of seismic retrofitting of RC structure is growing. Especially, the RC columns that are not reflected seismic design can not resist lateral loads by the earthquake. The brittle fracture of Non-seismic designed columns lead to full collapse of the building. Thus, the emergency columns reinforcement method is needed. That have a fast construction time, do not cause damage to the column. In the past, cross-sectional expansion method, a steel plate reinforcing method is applied mainly, but in recent years, carbon fiber sheet taking advantage of FRP (Fiber Reinforced Polymer) is widely used. In this study, retrofitting effect of seismic performance of FRP seismic reinforcement, which is possible to emergency construction, was examined. Reinforced concrete specimens were constructed to experimental study. The seismic performence of specimes retrifitted with FRP seismic reinforcement were evaluated. As a result, the seismic performance of specimen reinforced with FRP seismic reinforcement has been improved.

• Journal of the Korean Geotechnical Society
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• v.31 no.9
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• pp.69-78
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• 2015

If an active displacement occurs on a wall with finite size, the ground behind the wall forms shapes of 3-dimensional wedges and 3-dimensional active earth pressure are applied on the wall. In the previous studies, shapes of 3-dimensional wedges were measured and the resultant of 3-dimensional active earth pressure has been calculated. In this study, the magnitude and the distribution of 3-dimensional active earth pressure depending on the size of a rectangular wall, which was defined by the aspect ratio (h/w), that is, the ratio between the height and the width of wall, were measured and compared with previous 3-dimensional models. The result shows that, the horizontal displacement (S) of the wall is approx 0.12% of the height of wall (h). The resultant 3-dimensional active earth pressure is similar to that of Karstedt (1982). The distributions of earth pressures on the wall are parabolic shape. The peak earth pressure was measured at the 0.5~0.55 depth from the ground surface. The reduction factor of 3-dimensional active earth pressure against the 2-dimensional earth pressure (${\alpha}$) depending the aspect ratio (h/w) is presented by the diagram.