• Title/Summary/Keyword: 유전형질

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Adenosine Deaminase Gene: Possible Selectable Marker for Tobacco Transformation (연초의 형질전환을 위한 새로운 표지유전자로서 Mouse Adenosine Deaminase 유전자의 이용가능성)

  • 양덕춘;한성수;윤의수
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.4
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    • pp.235-240
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    • 1995
  • The development of selectable markers for transformation has been a major factor in the successful genetic manipulation of plant. We established a new selectable marker system for tobacco transformation using chimeric adenosine deaminase (ADA) gene, which confers resistance to cytotoxic adenosine analogues, 9-$\beta$-D-arabinofuranosyl adenine(Ara-A) and cordycepin. The transformants with the chimeric ADA gene in tobacco grew in the presence of normally lethal level of cytotoxic adenosine analogues, 100 $\mu$M Ara-A and 50 $\mu$M cordycepin. We successfully distinguished transformed shoot from non-transformed shoot on the same selectable media with cytotoxic adenosine analogues. In this selectable media, we were able to select seeds with/ without ADA gene from transgenic tobacco seeds. Theses results show that the mammalian ADA gene may serve as a new selectable marker for tobacco transformation.

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Estimation of Genetic Parameters for Linear Type and Conformation Traits in Hanwoo Cows (한우 암소의 선형 및 외모심사형질에 대한 유전모수 추정)

  • Lee, Ki-Hwan;Koo, Yang-Mo;Kim, Jung-Il;Song, Chi-Eun;Jeoung, Yeoung-Ho;Noh, Jae-Kwang;Ha, Yu-Na;Cha, Dae-Hyeop;Son, Ji-Hyun;Park, Byong-Ho;Lee, Jae-Gu;Lee, Jung-Gyu;Lee, Ji-Hong;Do, Chang-Hee;Choi, Tae-Jeong
    • Journal of agriculture & life science
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    • v.51 no.6
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    • pp.89-105
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    • 2017
  • This study utilized 32,312 records of 17 linear type and 10 conformation traits(including final scores) of Hanwoo cows in the KAIA(Korea Animal Improvement Association) ('09~'10), with 60,556 animals in the pedigree file. Traits included stature, body length, strength, body depth, angularity, shank thickness, rump angle, rump length, pin bone width, thigh thickness, udder volume, teat length, teat placement, foot angle, hock angle, rear leg back view, body balance, breed characteristic, head development, forequarter quality, back line, rump, thigh development, udder development, leg line, and final score. Genetic and residual(co) variances were estimated using bi-trait pairwise analyses with EM-REML algorithm. Herd-year-classifier, year at classification, and calving stage were considered as fixed effects with classification months as a covariate. The heritability estimates ranged from 0.03(teat placement) to 0.42(body length). Rump length had the highest positive genetic correlation with pin bone width(0.96). Moreover, stature, body length, strength, and body depth had the highest positive genetic correlations with rump length, pin bone width, and thigh thickness(0.81-0.94). Stature, body length, strength, body depth, rump length, pin bone width, and thigh thickness traits also had high positive genetic correlations.

Year Variations of Genetic Parameters Estimated on Maturity and Yield Characteristics (소맥의 숙기 및 수량관련형질에 대한 유전통계율의 년차간 변동)

  • Cho, C.H.;Sung, B.Y.;Ahn, W.S.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.25 no.3
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    • pp.15-20
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    • 1980
  • Heritability of heading date, maturing date and culm length were high and the year variation was relatively low. Yield components were considerably low in heritability due to large year variation. Direct effects of number of spikes/$m^2$ and 1, 000 grain weight to yield were high. For improved selection efficiency, at least three years continuous evaluation of experiments would be desired.

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Estimation of Genetic Parameters for Growth-Related Traits in 1-Year Old of Two Korean Abalone Subspecies, Haliotis discus hannai and H. discus discus, by Using Multiple Traits of Animal Model (다형질 Animal Model에 의한 12개월령 한국산 전북 2 아종의 성장관련형질에 대한 유전모수 추정)

  • Choe, Mi-Kyung;Han, Seock-Jung;Yang, Sang-Geun;Won, Seung-Hwan;Park, Choul-Ji;Yeo, In-Kyu
    • The Korean Journal of Malacology
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    • v.24 no.2
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    • pp.121-130
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    • 2008
  • In other aquaculture species, large improvements in growth have been achieved through selective breeding. Ezo abalone(Haliotis discus hannai) and disk abalone(H. discus discus) are major aquatic animals cultured in Asia, but selective breeding for the promotion of growth with these abalones has not been actively pursued. Recently significant efforts are being made to promote production of these species through selective breeding in Korea. The aims of this work were to estimate the general genetic parameters, heritabilities, and genetic and phenotypic correlations on growth-related traits at 1-year old in two Korean abalone subspecies, H. discus hannai and H. discus discus, by using multiple trait animal model. The data were collected from the records of 1,504 individuals produced from 22 sires and 26 dams in H. discus hannai and 297 individuals produced from 5 sires and 6 dams in H. discus discus, which evaluated by the Genetics and Breeding Research Center, National Fisheries Research & Development Institute(NFRDI). Genetic parameters were estimated for these abalone subspecies raised in Bukjeju branch, NFRDI, from May 20, 2004 to May 16, 2005, respectively. The heritability estimates obtained from restricted maximum likelihood(REML) were higher than expected, ranging from 0.40 to 0.43 for growth traits shell length, shell width and body weight in H. discus hannai and from 0.26 to 0.51 in H. discus discus, respectively. The heritabilities for shell shape and condition factor were lower than others of growth traits such as ranging from 0.09 to 0.19 in H. discus hannai and from 0.10 to 0.23 in H. discus discus, respectively. Genetic and phenotypic were > 0.93 between shell parameters and weight in two abalone species, respectively, indicating that breeding for weight gains could be successfully achieved by selecting for shell length.

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Estimation of Genetic Parameters for Ultrasound and Carcass Traits in Hanwoo (한우의 초음파 측정 형질과 도체 형질의 유전모수 추정)

  • Kim, Hyeong-Cheol;Lee, Seung-Hwan;Dang, Chang-Gwon;Jeon, Gi-Jun;Yeon, Seong-Heum;Cho, Young-Moo;Lee, Sang-Min;Yang, Boh-Suk;Kim, Jong-Bok
    • Journal of Animal Science and Technology
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    • v.54 no.5
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    • pp.331-336
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    • 2012
  • This study was conducted to estimate genetic parameters for ultrasound and carcass traits in Hanwoo. Heritabilities and genetic and phenotypic correlations were estimated for carcass and ultrasound measurements collected from Hanwoo cows (n=312) born at Hanwoo experiment station. Traits evaluated were eye muscle area (EMA), backfat thickness (BF), marbling score (MS) from carcass, and ultrasound eye muscle area (UEMA), ultrasound backfat (UBF), and ultrasound marbling score (UMS). Parameters were estimated using multi-trait animal models byderivative-free restricted maximum likelihood procedures. Estimated heritabilities for UBF, UEMA and UMS were 0.43, 0.23 and 0.32, while heritabilities for BF, EMA and MS were 0.33, 0.13 and 0.33 in fattened cows, respectively. Genetic correlations between ultrasound and carcass measurements were estimated to -0.19, -0.61, and -0.36 for UBF: UEMA, UBF: UMS, and UEMA: UMS in fattened cows, respectively. Phenotypic correlations between ultrasound and carcass measurements were 0.03, 0.13 and 0.26 for UBF: UEMA, UBF: UMS, and UEMA: UMS in fattened cows, respectively. As for the steer, genetic correlations between ultrasound and carcass measurements were 0.36, -0.80 and 0.27 for UBF: UEMA, UBF: UMS, and UEMA: UMS in steers, respectively. Phenotypic correlations between ultrasound and carcass measurements were 0.13, 0.07 and 0.41 for UBF: UEMA, UBF: UMS, and UEMA: UMS in steers, respectively. In conclusion, this finding would be very useful to implement into Hanwoo breeding program.

Studies on the selection in soybean breeding. -II. Additional data on heritability, genotypic correlation and selection index- (대두육종에 있어서의 선발에 관한 실험적연구 -속보 : 유전력ㆍ유전상관, 그리고 선발지수의 재검토-)

  • Kwon-Yawl Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.3
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    • pp.89-98
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    • 1965
  • The experimental studies were intended to clarify the effects of selection, and also aimed at estimating the heritabilities, the genotypic correlations among some agronomic characters, and at calculating the selection index on some selective characters for the selection of desirable lines, under different climatic conditions. Finally practical implications of these studies, especially on the selection index, were discussed. Twenty-two varieties, determinate growing habit type, were selected at random from the 138 soybean varieties cultivated the year before, were grown in a randomized block design with three replicates at Chinju, Korea, under May and June sowing conditions. The method of estimating heritabilities for the eleven agronomic characters-flowering date, maturity date, stem length, branch numbers per plant, stem diameter, plant weight, pod numbers per plant, grain numbers per plant and 100 grain weight, shown in Table 3, was the variance components procedures in a replicated trial for the varieties. The analysis of covariance was used to obtain the genotypic correlations and phenotypic correlations among the eight characters, and the selection indexes for some agronomic characters were calculated by Robinson's method. The results are summarized as follows: Heritabilities : The experiment on the genotype-environment interaction revealed that in almost all of the characters investigated the interaction was too large to be neglected and materially affected the estimates of various genotypic parameters. The variation in heritability due to the change of environments was larger in the characters of low heritability than in those of high heritability. Heritability values of flowering date, fruiting period (days from flowering to maturity), stem length and 100 grain weight were the highest in both environments, those of yield(grain weight) and other characters were showed the lower values(Table 3). These heritability values showed a decreasing trend with the delayed sowing in the experiments. Further, all calculated heritability values were higher than anticipated. This was expected since these values, which were the broad sense heritability, contain the variance due to dominance and epistasisf in addition to the additive genetic variance. Genotypic correlations : Genotypic correlations were slightly higher than the corresponding phenotypic correlations in both environments, but the variation in values due to the change of environment appeared between grain weight and some other characters, especially an increase between grain weight and flowering date, and the total growing period(Table 6). Genotypic correlations between grain weight and other characters indicated that high seed yield was genetically correlated with late flowering, late maturity, and the other five characters namely branch numbers per plant, stem diameter, plant weight, pod numbers per plant and grain numbers per plant, but not with 100 grain weight of soybeans. Pod numbers and grain numbers per plant were more closely correlated with seed yields than with other characters. Selection index : For the comparison and the use of selection indexes in the selection, two kinds of selection indexes were calculated, the former was called selection index A and the later selection index B as shown in Table 7. Selection index A was calculated by the values of grain weight per plant as the character of yield(character Y), but the other, selection index B, was calculated by the values of pod numbers per plant, instead of grain weight per plant, as the character of yield'(character Y'). These results suggest that selection index technique is useful in soybean breeding. In reality, however, as the selection index varies with population and environment, it must be calculated in each population to which selection is applied and in each environment in which the population is located. In spite of the expected usefulness of selection index technique in soybean breeding, unsolved problems such as the expense, time and labor involved in calculating the selection index remain. For these reasons and from these experimental studies, it was recognized that in the breeding of self-fertilized soybean plants the selection for yield should be based on a more simple selection index such as selection index B of these experiments rather than on the complex selection index such as selection index A. Furthermore, it was realized that the selection index for the selection should be calculated on the basis of the data of some 3-4 agronomic characters-maturity date(X$_1$), branch numbers per plant(X$_2$), stem diameter(X$_3$) and pod numbers per plant etc. It must be noted that it should be successful in selection to select for maturity date(X$_1$) which has high heritability, and the selection index should be calculated easily on the basis of the data of branch numbers per plant(X$_2$), stem diameter(X$_3$) and pod numbers per plant, directly after the harvest before drying and threshing. These characters should be very useful agronomic characters in the selection of Korean soybeans, determinate growing habit type, as they could be measured or counted easily thus saving time and expense in the duration from harvest to drying and threshing, and are affected more in soybean yields than the other agronomic characters.

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Major genotype identification affecting economic traits in FABP4, SCD, FASN and SREBPs genes of Korean cattle (한우의 FABP4, SCD, FASN, SREBPs 유전자에서 경제형질에 영향을 미치는 우수 유전자형 선별)

  • Lee, Jea-Young;Park, Jae-Cheol
    • The Korean Journal of Applied Statistics
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    • v.29 no.7
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    • pp.1247-1255
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    • 2016
  • Kim and Lee (2015) identified a superior FABP4 gene that improves the grade and fatty acid of Korean cattle. This study selects a superior genotype by expanding genes that influence the economic traits of Korean cattle. Expanded genes are FABP4, SCD, FASN and SREBPs that are related to grade and fatty acid (Oh, 2014). We use the adjusted economic-trait values with environmental factors excluded. We also applied multifactor dimensionality reduction(MDR) method to data of the adjusted economic-trait values. As a result, we identified superior genes and genotypes which improved the grade and fatty acid of Korean cattle.

Stable Transmission and Expression of TPO Transgene up to 10 Generation in the Transgenic Mice (형질전환 생쥐에서 제10세대까지 TPO 유전자의 안정적 전이와 지속적인 발현)

  • 정진우;오건봉;한용만;이경광
    • Korean Journal of Animal Reproduction
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    • v.27 no.1
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    • pp.9-14
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    • 2003
  • The pBT-L transgenic mice carrying human TPO gene in conjunction with bovine $\beta$-casein promoter express human TPO in milk during lactation. In this study, stability of germ line transmission and expression of pBT-L transgene integrated into host chromosome were monitored up to generation F10 of transgenic pBT-L/15 line. When male mouse of generation F8 was crossbred with normal females, approximately half of offsprings (51.3$\pm$18.98%) were identified as transgenic mice. Generation F9 and F 10 mice also showed similar transmission rates (43.8$\pm$18.98% and 71.4$\pm$26.98%, respectively), implying that pBT-L transgene can be transmitted stably up to long term generation in the transgenic mice. Expression levels of human TPO from milk of generation F9 and F10 mice were 1.1$\pm$0.33 mg/ml and 1.1 $\pm$0.45 mg/ml, respectively, which are similar to expression level of generation F2 mice. In conclusion, our results suggest that transgenic animals once established will continuously pass their transgenes to the progeny through the breeding program with the same productivity of human protein in their milk.

Local Variation of Genetic Parameters of Arachis Hypogaea, L. (땅콩(Arachis Hypogaea, L.) 품종들의 주요형질에 대한 유전통계량의 지역간 변동)

  • Jung-Il Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.240-247
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    • 1983
  • Heritability of flowering date, length of main stem, weight of 100 grains and number of shells per square meter in peanut were high with low local variation. There was positive genotypic correlation between length of main stem and yield, No. of shells per square meter and matured seed ratio, No of shells per square meter and yield 100 grain weight and yield but was negative genotypic correlation between flowering date and yield. With the view of path-coefficients, length of main stem, number of shells per square meter showed highly direct effects at.. all locations.

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Studies on Heterosis Selective Index, Genetic Advance and Selective Efficiency for some Characters in Silkworm Breeding (누에 육종에 있어서 잡종강세, 선발지수, 유전진전, 선발효율에 관한 연구)

  • 정원복
    • Journal of Sericultural and Entomological Science
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    • v.32 no.1
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    • pp.8-16
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    • 1990
  • The experiment was conducted to obtain estimates of heterosis, selective index, genetic advance and selective efficiency in cross among silkworm varieties. Seven parents, 21 F$_1$ hybrids, and 21 F$_2$ populaltions from diallel crosses among them were evaluated. The results obtained are summarized as follows: In male and female of F$_1$ generation, the negative heterosis was shown in two characters of fifth instar period and boiling on ratio, and the positive heterosis with the value of 20.90-37.60% in the other characters. In those of F$_2$, the nagative heterosis was shown in two characters of cocoon layer ratio and boiling off ratio, and the positive heterosis in the other characters. The selection weight of cocoon weight for bave weight was the highest of those of all the characters, and that of the combination of the fifth instar period and cocoon weight for it was the highest of those of all the combination. The genetic advance of cocoon weight toward bave weight was the highest of all the characters. The highest genetic advance and selective efficiency were shown in the combination of all the characters.

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