• Journal of Food Hygiene and Safety
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• v.27 no.2
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• pp.146-151
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• 2012

In this study, effective gene extraction methods were compared to identify raw materials of processed meat products through molecular biological methods. Species specific primers were used to identify ingredients of processed foods and, as a sample, 13 kinds of processed meat products including beef, pork and chicken. According to the type of sample, 13 kinds of samples were classified into liquid type, source type and powder type. The samples were pre-treated (centrifugation) and (or) performed Whole Gene Amplification (WGA) kit for amplification of the extracted DNA. As a result, it was possible to identify the raw material of products through the centrifugation of sample 1 ml for liquid type of processed meat products. For source type of products after gene extraction, it was required to perform WGA for the identification of ingredients. For powder type products did not required any further pre-treatment and WGA. In this study, it was an opportunity to confirm the possibility of identification of raw material from the gene extraction of processed meat products and this method could be used to examine the authenticity of raw material of products.

• Journal of Sericultural and Entomological Science
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• v.47 no.2
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• pp.51-55
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• 2005

Resveratrol is naturally occurring phytoalexin compounds produced by grape berries, peanuts, and their products in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to develope a reliable high performance liquid chromatographic (HPLC) method for the quantitative determination of trans-resveratrol in mulberry fruit. Samples were extracted in 80% MeOH and filtered with $0.45{\mu}m$ syringe filter. The transresveratrol was separated Waters $C_{18}$ column, using a mobile phase containing 0.025% trifluroacetic acid in 5% acetonitril and 0.035% trifluroacetic acid in 50% acetonitril, detected by photodiode array detector (PDA) at 254 nm and the flow rate was 1ml/min. Under this analytical condition, the mean content of mulberry fruits (fifty varieties) was $777.3{\pm}585.9ppm$. Among the tested samples, 'Mansaengbaekpinosang (II)' was the highest level in 3450.6 ppm. However four accessions including 'Gukbu', 'Sabangso (I)', 'Simseol' and yield mulberry fruit were not able to detected. Eight suitable varieties selected for the production of fruit were 'Jeolgokchosaeng (Chungbuk)' 777.8 ppm, 'Dangsang 7' 771.1 ppm, 'Jangsosang' 133.9 ppm, 'Susungppong' 31.1 ppm, 'Suwonnosang' 639.7 ppm, 'Palcheongsipyung' 1475.9 ppm, 'Kangsun' 864.0 ppm, and 'Jukcheonchosaeng' 1458.5 ppm. 'Daesungppong' which was the first authorized variety for the production of mulberry fruit was 1236.7 ppm. In conclusion, these results suggest that mulberry including fruit and leaf may a good new resource for resveratrol production.

• Korean Journal of Animal Reproduction
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• v.21 no.2
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• pp.167-176
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• 1997

This study was carried out to evaluate the potential for preselection of transgenic embryos prior to transfer into recipient animals. In these experiments, I used a 3.2 kb transgene which contained the neomycin resistance gene (neo) and lac Z gene driven by the $\beta$ actin promoter (iacZ Ineo). Oocytes were aspirated from abattoir ovaries, matured in TCM-199 supplemented with 10% fetal bovine serum (FBS), 5 ${\mu}\textrm{g}$/ml LH, 0.5 ${\mu}\textrm{g}$/ml FSH, 100 unit/ml penicillin, and 100 ${\mu}\textrm{g}$/ml streptomycin for 22 to 24 hrs then inseminated with a sperm suspension of 1 X 10$^6$ sperm/ml containing 5 ${\mu}\textrm{g}$/ml of heparin. At 18-20 hrs after insemination, cumulus cells were removed by vortexing and pronuclei of centrifuged zygotes microinjected with the lacZ/neo construct (3 ng/$\mu$l). All cultures were carried out in CR1aa with transfected BRL monolayers containing 3 mg/ml BSA, 20 $\mu$/ml NEM amino acids and 40 $\mu$I/ml BME amino acids. To identify the appropriate concentration of G418 for selection, non-microinjected zygotes were cultured in the presence of 0, 50, 100 and 200 $\mu$g/ml of G418. After 8 days of culture in these treatments, 44/145 (30.3%), 13/150 (8. 7%), 1/151 (0.7%) and 0/134 (0.0%) devel-oped to the blastocyst stage in 0, 50, 100 and 200 $\mu$g/ml of G418, respectively. A total of 1,127 zygotes were microinjected and placed into culture (without G418) and subsequently 710 (63.0%) cleaved. At 48 hrs post-injection, embryos ($\geq$2-cell) were randomly assigned to control (medium alone) or G418 (100 ${\mu}\textrm{g}$/ml) treatments. A control culture of 740 non-microinjected embryos from the same replicates of embryos were also placed into control medium. After 8 days in culture, 54/343 (15.7%) and 22/367 (6.0 %) of the microinjected embryos developed to the blastocyst stage in control and G418 media, respectively. A total of 151/740 (27.2%) of the non-microinjected embryos placed in the control medium developed to the blastocyst stage. The blastocysts in the control treatment had a mean of 70.7 ${\pm}$ 4.7 cells of which 23.1 ${\pm}$ 2.6 (32.7%) stained for $\beta$-Gal activity. B1astocysts in the G418 treatment had a mean of 48.8${\pm}$7.5 cells of which 40.3 ${\pm}$ 4.1 (82.6%) stained for $\beta$-Gal ac tivity (P<0.05). In the control treatment 26 of 30 (87.0%) blastocysts had some cells with $\beta$-Gal activity while all of the blastocysts in the G418 treatment had some cell with $\beta$-Gal ac tivity (P<0.05). However, ICM colonies in either control or G418 treatments were not expressed any epiblast cell except of trophetoderm celIs. The $\beta$-actin promoter/lacZ gene may not be e expression or silence expression in epiblast cells These results clearly show an enrichment of blastocysts expressing the transgene in the majority of their cells after culture in the presence of G418. The exogeneous gene was not express a and silence in ICM colonies especiallly epiblast cells except of trophectederm cells. Even though the higher rate cell number expressed of exogeneous gene in the G418 treatments, a total cell number was decrease significantly (P<0.05) of which might be also drop of the establishment of ES like-cell colonies and production of transgenic animals. However, futher studies need to determine the viability of these selected embryos and the avability of production of transgenic offspring.

• Journal of Korean Society of Forest Science
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• v.47 no.1
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• pp.37-43
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• 1980

This study have done to get the basical information that would be useful to make the ecological planting, selection of suitable species and weeding plan by the relation between the leading shoot elongation of several species and the climatic factors in the plantation. Sampling measurement have been done in the trial forest of Korean German Forest Management Project located in Joil-ri, Samnam-myeon and Ichcon-ri, Sangbug-myeon, Ulju-gun. The former is in lowland at 100m latidude and the latter is in highland of 600 m latitude. The elongation of leading shoot has been measured in the plantation with 10 days interval from the beginning of March in 1979 and the climatic datas has gotten in the weather station closed to the plantation. 1. The change of air temperature and rainfall in each measuring site is like Fig 1. and 2. The similar temperature in 600 m high latitude is coming about 10 days latter than 100 m latitude. 2. Genus pine as Pinus thunbergii, P. rigida, P. rigitaeda. P. koraiensis and P. taeda begin their leading shoot growth during March and air temperature in that time is around $6^{\circ}C$. In highland their beginning of leading shoot elongation has been found out 10 days latter than lowland. However Abies, Larix and Picea has shown to open their leading shoot during May, 40 days late in comparing with genus pine, and then temperature is making around $15^{\circ}C$. But Cryptomeria, Chamaecyparis and Cedrus deodora has shown their leading shoot opening in March in lowland and May in high land. The reason of late opening, specially in highland, seems to be the influence of winter frost. 3. Most of leading shoot elongation of genus pine has finished during the end 10 days of April and May under range of air temperate $10^{\circ}C$ and $20^{\circ}C$ and other species has finished most of their elongation during the end 10 days of May and June with air temperature range of $18^{\circ}C$ to $20^{\circ}C$. So the suitable season of weeding works show to genus pine in May and other species in June. 4. The leading shoot growth of genus pine has started earlier and closed earlier too than other species and, when over than $20^{\circ}C$ air temperature, their growth is decreasing quickly. Pices abies as well show to be decreased suddenly in over than $20^{\circ}C$ temperature. Other species show the similar trend when over than $22^{\circ}C$. This reason is considered as high temperature of summer season. 5. Annual elongated days of leading shoot of Picea abies is 50 days, Abies hollophylla 70 days, and more than 85 percentage of shoot growth of Pinus koraiensis and Larix leptolepsis are growing during 70 dys as well. The shoot growing days of Chamaecyparis, P. rigida, P. rigitaeda, P. taeda and P. shunbergii show longer period as over than 120 days. 6. The shoot elongation times per year of Abies and Picea has closed as one times and Genus pine is continuring their elongation more than two times. But Cryptomeria, Chamaecyparis, Cedrus deodora and Larix show one or two times elongation depending on the measuring site. The reason of continuring elongation more than than two times seems to be influenced by the temperature in summer season except the genetical reason. 7. Depending on the above results, as the high temperature in summer season could give the influence to grow the leading shoot in the plantation, this would be the considering point on the ecological planting and selection of the suitable species to the slope aspect. The elongation pattern by the season show to be the considering point too to decide the the weeding and fertilizer dressing time by the species.