• Journal of Life Science
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• v.14 no.5
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• pp.874-881
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• 2004

Angiogenesis has been implicated in progression of inflammation, arthritis, psoriasis, atherosclerosis as well as tumor growth and metastasis. Intensive studies have been carried out to develop a strategy for cancer treatment by blocking angiogenesis. During angiogenesis, endothelial proliferation and migration essentially occurs upon activation. In this study, we compared the expression profiles of human umbilical endothelial cells activated by incubating in vitro in the rich medium containing several growth factors, and non-activated ones. cDNA targets derived from total RNAs of HUVEC activated for 13 h in M199 medium containing endothelial cell growth supplement, 20% fetal bovine serum, and heparin, after reaching 70～80% confluency, or non-activated, were hybridized onto oligonucleotide microarrays containing 1,8864 genetic elements. Unsupervised hierarchical clustering analysis resulted in two subgroups on dendrogram exhibiting activated and non-activated HUVECs. We then extracted 122 outlier genes which were shown to be up-regulated or under-expressed by at least 2-folds in activated HUVECs. Among these, 32 annotated genes were up-regulated and 38 were down-regulated in activated HUVECs. Interestingly, genes involved in cell proliferation, motility, and inflammation/ immune response were up-regulated in activated HUVEC, whereas genes for cell adhesion or vessel morphogenesis/function were down-regulated. Unexpectedly, the expression of genes well-characterized as angiogenesis markers was not changed except Eph-B4, which was down-regulated about 4 folds. 52 unknown genes were also up- or down-regulated. Therefore, these results could provide an opportunity to targeting new vascular molecules for the development of anti-angiogenic molecules.

• Korean journal of applied entomology
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• v.53 no.3
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• pp.199-207
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• 2014

It is true that the proper environmental risk assessments for many GM (Genetically Modified) insects almost have not been executed in Korea. Therefore, we tested the environmental risk assessment about GM silkworms if there is any difference between GM silkworms and non-GM silkworms by the following three measurements. First, we measured their mobility in the breeding environment conditions with food and without food. Secondly, we measured their viability at the artificial extreme environmental conditions (low and high temperature and humidity, absent/present of foods,) after escaping from their breeding environments. Thirdly, we observed the number of laying eggs and their hatchability between GM silkworms and non-GM silkworms with four different pair experiments. The mobility of GM silkworms and non-GM silkworms statistically did not differ, and the egg productivity and hatchability were not also different. The hatchability by couple of GM female silkworms and non-GM male silkworms was lower than by non-GM male and female couple between the GM silkworms and non-GM silkworms, and there was statistically different. Relatively, the viability of GM silkworms was lower than non-GM silkworms. We could not exactly test for viability of silkworms in low temperature conditions because of their hibernating. Although there was any difference in viability and hatchability between GM silkworms and non-GM silkworms, all ability of GM silkworms was lower than non-GM silkworms. Conclusively, the environmental risk of GM silkworm was relatively lower than non-GM silkworm in this study.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.39-45
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• 2000

Two maize transposable elements, immobilized Ac (iAc) and Ds, have been introduced into the genome of a diploid potato clone (Solanum tuberosum Group Phureja clone 1.22). The iAc is a modified Ac that is supposed to be unable to transpose but is expected to trans-activate the transposition of a Ds that is unable to transpose by itself. When the leaf and stem explants of in vitro shoots of the clone 1.22 were inoculated with Agrobacterium tumefaciens strains harboring binary vectors containing the iAc and the Ds, calli were formed from the explants on media containing 50 mg/L of kanamycin, and shoots were regenerated from the calli. The regenerated shoots formed roots when cultured on media containing 100 mg/L of kanamycin, whereas untransformed shoots did not form roots on the same media. The PCR amplification of the DNA's from the transgenic plants confirmed that the iAc and the Ds elements were introduced into the potato genome of 1.22.

• Proceedings of the Korea Water Resources Association Conference
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• 2000.05a
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• pp.829-834
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• 2000

• Korean journal of applied entomology
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• v.39 no.1
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• pp.43-52
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• 2000

A portion of mitochondria1 COI gene (438 bp) was sequenced from the sampls of Plutella xylostella from four localities in Korea to investigate the population genetic structure and characteristics by measuring the magnitude of genetic diversity and the degree of gene flow among populations. Thirteen haplotypes ranging in nucleotide divergence 0.3% to 1.4%, were obtained from 21 individuals. The nucleotide divergence was similar to the other related studies, but haplotype diversity was substantially higher (mean h = 0.81). The genetic distance among geographically remote Cheju Island population and the two Kimhae populations, distant 1 lkm to each other, was not statistically significant (p<0.05). Instead, a substantial or high female gene flow was detected (Nm = 2-30). One Hawaiian haplotype of the diamondback moth obtained through GenBank search also was genetically similar to the ones obtained from this study. Collectively, the genetic population structure of the diamondback moth in Korea can be characterized into two aspects. First, the diamondback moths in Korea possesses overall moderate genetic divergence based on a high number of haplotypes. Second, a high haplotype diversity within each population due to the long distance dispersal with a substantial dispersal power and the resultant genetic similarity among geographic populations is characteristic.

• Korean Journal of Weed Science
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• v.32 no.1
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• pp.10-16
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• 2012

This study was carried out to investigate the agronomic traits, comparison of weed characteristics and possibility of gene flow in 'vitamin A enforced GM rice' and the donor plant, 'Nagdong'. The GM rice was not significantly different agronomic traits compared to the donor plant, Nagdong. Weed population changes were investigated in the cultivation of the GM rice and the donor plant, Nagdong. Dominant weed species and their dry matter did not show the difference between GM rice and the donor plant, Nagdong in macro-GM crop field. Dominant weed species with the GM rice and the donor plant, Nagdong were Monochoria vaginalis, followed by Eleocharis kuroguwai, Echinochloa crus-galli and Lindernia procumbens. The detection of gene from the GM rice was done using PCR, gene flow can't be detected by weed species. Results of this study on the agronomic traits, weed characteristics and possibility of gene flow has elucidated that GM rice might not be different from the donor plant, Nagdong.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2008.04a
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• pp.60-63
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• 2008

본 논문에서는 신경망 모델(neural network model)과 유전자 알고리즘(genetic algorithm)을 이용한 실시간 경로 계획(real-time path-planning)과 퍼지 룰(fuzzy rule)을 이용한 효율적인 다중경로계획(multiple path-planning)을 제안한다. 실시간 경로 계획은 빠른 시간 내에 최적 경로의 생성이 반드시 수행되어야 하므로, 본 논문에서는 경로 계획 중 장애물 지역과 비장애물 지역을 빠르게 확인하기 위해 신경망 모델을 이용하여, 이동 방향 및 최적경로 탐색을 위하여 유전자 알고리즘을 이용하였다. 또한 충돌 구역에서의 효율적인 다중 경로 계획을 위해, 퍼지를 이용하여 경로를 재계획 하였다. 퍼지의 경우, 현재 위치에서 목표 지점으로의 방향을 계산하기 위한 퍼지 소속 함수와 현재 위치와 충돌 구역까지의 거리 값을 가중치로 세우고 퍼지 룰을 결정하여 경로계획을 수행하였다. 시뮬레이션을 통해 실험해본 결과, 퍼지 룰을 사용했을 때 사용하지 않았을 때 보다 좋은 성능을 나타남을 확인할 수 있었다.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.4
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• pp.642-648
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• 2000

We introduce a new application of the genetic algorithm for design of the coaxial fed circularly polarized rectangular microstrip antenna. The GA shows excellent results for the design of the microstrip antenna which have a very complex objective function including the input impedance, the effective loss tangent and the axial ratio. The objective function is derived from the cavity model, and the size and the feeding point are optimized by the genetic algorithm. The experimental results show good agreement with the simulated results.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2005.04a
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• pp.305-308
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• 2005

본 논문은 유전자 알고리즘 기반 퍼지 다항식 뉴럴네트워크(Genetic Algorithm-based Fuzzy Polynomial Neural Networks ; GAs-based FPNN)를 이용하여 비선형 데이터의 최적화 추론 알고리즘을 제안한다. FPNN의 각 노드는 GMDH와 퍼지규칙을 기초로 만들었다. FPNN의 각 노드는 퍼지 다항식 뉴론(Fuzzy Polynomial Neuron : FPN)이라고 표현하다. 제안된 모델은 구조 선택에 있어서 유전자 알고리즘(Genetic Algorithms : GAs)을 이용하였다. 유전자 알고리즘을 사용하여 입력의 차수와 입력의 개수 그리고 후반부 추론의 형태를 최적 선택하였다. 비선형 데이터에 대한 모델 설계를 위해 최적화 알고리즘인 유전자 알고리즘 기반 FPNN 모델 설계가 유용하고 효과적임을 보인다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.805-807
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• 2017

유전자 알고리즘을 이용하여 스스로 테트리스 게임을 플레이하는 AI 기법을 제안한다. 테트리스에 필요한 요소들을 고려하여 각 요소마다 가중치를 곱한 값을 통해 블록을 이동시킬 자리를 정한다. 해당 알고리즘은 8가지의 고려 요소를 가지며, 각 요소별 최적의 가중치를 구하기 위해 유전자 알고리즘을 적용하였다. 본 연구의 성능을 분석하기 위하여 직접 설계 제작한 테트리스로 게임을 정확하게 진행해 나가는가를 실험하였다. 실험 결과, 제안 기법에 따라 테트리스를 진행하는 것을 확인하였다.