• 대한임상약리학회:학술대회논문집
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• 2006.11a
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• pp.91-91
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• 2006

• Journal of Mushroom
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• v.15 no.2
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• pp.78-83
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• 2017

Microsatellite SSR markers were developed and utilized to reveal the genetic diversity of 32 strains of Flammulina velutipes collected in Korea, China, and Japan. From the SSR-enriched library, 490 white colonies were randomly selected and sequenced. Among the 490 sequenced clones, 85 (17.35%) were redundant. Among the remaining 405 unique clones, 201 (49.6%) contained microsatellite sequences. We used 12 primer pairs that produced reproducible polymorphic bands for four diverse strains, and these selected markers were further characterized in 32 Flammulina velutipes strains. A total of 34 alleles were detected using the 12 markers, with an average of 3.42 alleles, and the number of alleles ranged from two to seven per locus. The major allele frequency ranged from 0.42 (GB-FV-127) to 0.98 (GB-FV-166), and values for observed ($H_O$) and expected ($H_E$) heterozygosity ranged from 0.00 to 0.94 (mean = 0.18) and from 0.03 to 0.67 (mean = 0.32), respectively. SSR loci amplified with GB-FV-127 markers gave the highest polymorphism information content (PIC) of 0.61 and mean allele number of five, whereas for loci amplified with GB-FV-166 markers these values were the lowest, namely 0.03 and two. The mean PIC value (0.29) observed in the present study with average number of alleles (3.42). The genetic relationships among the 32 Flammulina velutipes strains on the basis of SSR data were investigated by UPGMA cluster analysis. In conclusion, we succeeded in developing 12 polymorphic SSRs markers from an SSR-enriched library of Flammulina velutipes. These SSRs are presently being used for phylogenetic analysis and evaluation of genetic variations. In future, these SSR markers will be used in clarifying taxonomic relationships among the Flammulina velutipes.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.6 no.2
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• pp.112-119
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• 2003

Purpose: Helicobacter pylori infection is thought to be correlated with iron-deficiency anemia (IDA) at puberty. The H. pylori feoB gene, a high-affinity ferrous iron transporter, plays a central role in iron acquisition. This study aims to analyze the H. pylori feoB status according to the presence of antral gastritis with or without IDA. Methods: Fourteen H. pylori-positive patients aged from 10~18 years were categorized into subgroups based on the presence or absence of IDA. Eight patients had IDA, and the other six showed normal hematological findings. Genomic DNA was isolated from cultured H. pylori. Five sets of primers were used for PCR amplification of the feoB gene. The feoB region, 1.93 kb, was generated by linking of the PCR products and sequenced. The feoB gene sequences of H. pylori J99 and 26695 were used to compare with the clinical strains. Sequence comparisons of the feoB regions between the IDA (+) and (-) groups were performed. Results: Sequence analysis of the complete coding region of the feoB revealed 16 sites of polymorphism. Among these, 3 polymorphisms-Glu/Thr254Ala, Ile263Val, and Lys511Gln - were indigenous to Korean strains. Although statistically significant differences appear in 4 sites between IDA (+) and (-), the number of specimens are too low to assess the real differences. Conclusion: The 4 polymorphisms in the feoB gene seem to be related with IDA, but it is unclear yet because of small number of study strains. Further studies are required to prove the correlation of IDA and H. pylori infection.

• Journal of Nutrition and Health
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• v.41 no.8
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• pp.767-775
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• 2008

Uncoupling protein-1 (UCP-1) plays a major role in thermogenesis at brown adipose tissues and has been implicated in the pathogenesis of obesity and metabolic disorders. The purpose of this study was to estimate the effects of A-3826G polymorphism in 117 Korean prepubertal children aged 8-11 years olds. Anthropometry by bioelectrical impedance analysis method, plasma lipid profiles by auto-biochemical analyzer and UCP-1 genotyping by PCR-RFLP were done. The frequencies of UCP-1 genotypes were AA; 17.7%, AG; 57.8%, GG; 26.6%. The frequencies of each G allele (55.5%) was similar to Japanese's (49%) and higher than Caucacian's (25%). No correlation UCP-1 polymorphism and BMI (kg/$m^2$) or the degree of obesity described by the relative percentiles of the standard weight according to height in prepubertal children. However, plasma total- and LDL-cholesterol were significantly increased in G allele when sex, age and weight were adjusted. Our results suggested that G allele of UCP-1 gene was stronger risk factors in hyperLDLcholesterolemia than A allele. This impact might be progressed as the precaution against the revalence of obesity based-metabolic disease.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2003.10a
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• pp.201-205
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• 2003

유전자 감식법은 1980년대 후반에 미국에서 처음으로 법의학적으로 적용된 이래, 강력범죄, 친자확인 및 항공기 추락사고, 화재 등의 대형 참사의 신원 확인에 큰 공헌을 하였다. 유전자감식을 위해서는 높은 다형성을 보이는 STR (short tandem repeats) 좌위의 타이핑을 주로 이용한다. 한편, 최근 줄기세포 (stem cell)의 중요성이 인식되면서 폐기되던 제대혈의 보관 사업이 활성화되고 있다. 본 연구에서는 제대혈의 보관시 STR 타이핑의 유전 정보를 표기한 유전자 캐릭터를 동시에 제공하여 불의의 사고시 신원확인이 가능하게 하고, 아울러 이산가족에 대한 DNA정보 데이터베이스 구축 및 검색 프로그램을 개발하였다.

• 대한예방의학회:학술대회논문집
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• 2001.04a
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• pp.91-91
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• 2001

• 대한예방의학회:학술대회논문집
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• 1999.10a
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• pp.138-139
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• 1999

• Proceedings of the Korean Nutrition Society Conference
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• 2002.05a
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• pp.143-143
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• 2002

• Proceedings of the Korean Nutrition Society Conference
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• 2003.10a
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• pp.108-108
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• 2003

• Journal of Acupuncture Research
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• v.19 no.4
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• pp.183-189
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• 2002

목적 : 본 연구는 catalase 유전자 다형성이 중풍의 발병과 관련이 있는지 알아보기 위해 수행되었다. 대상 : 경산대학교부속구미 한방병원에 입원한 중풍환자 86 명과 종합건진센터에 내원한 중풍기왕력이 없는 건강인 165 명을 대상으로 하였다. 방법 : 각 그룹에서 개개인마다 DNA를 분리 정제한 후 Taq polymerase로 증폭하여 한천 겔에서 전기영동을 하여 잘려진 DNA fragment의 양상을 관찰하였다. 결과 : T/T, T/A, A/A의 세가지 유전자형이 검출되었으며 중풍군과 건강인 사이에 통계적인 유의성이 나타나지 않았다. 결론 : 이상의 결과를 통하여 catalase 유전자 다형성은 중풍의 발병과는 관련성이 없는 것으로 사려되며 더 많은 환자를 대상으로 다른 환경요인 또는 유전자와의 연관성에 대한 심도있는 연구가 필요하다고 하겠다.