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Dynamics of Phytoplankton and Zooplankton of a Shallow Eutrophic Lake (lake llgam) (수심이 얕은 부영양 인공호(일감호)의 동 ${\cdot}$ 식물플랑크톤 동태학)

  • Kim, Ho-Sub;Park, Je-Chul;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.36 no.3 s.104
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    • pp.286-294
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    • 2003
  • This study was attempted to understand seasonal dynamics of phyto- and zooplankton communities in shallow, eutrophic Lake llgam and to compare them with the PEG (Plankton Ecology Group) model. Seasonal succession pattern of phytoplankton community was similar to PEG model as Chlorophyceae and Baciliphyceae increase during spring and autumn fellowed by increase of Cyanophyceae. However, based on the cell density and biomass, a dominant phytoplankton community differed with PEG model: Cyanophyceae had been a dominant community throughout a year, except for ice-cover period during which Chlorophyceae was a dominant group. In spring, when ice melted and dissolved nutrients in water column increased, the increase of Chlorophyceae occurred: when nutrients (DIN and DIP) rapidly decreased, Cyanophyceae increase occurred. Microcystis, Oscillatoria, Lyngbya, Merismopedia were maior dominant species of Cyanophyceae and their cell density and/or biomass was the highest in October 2000 (12.9${\pm}$5.8${\times}10^5$ cells/ml, 3.5${\pm}$0.9${\times}10^3{\mu}gC/l$). Cyanophyceae biomass showed positive relationship with chlorophyll a ($r^2$ = 0.71,P< 0.001) and TP concentration ($r^2$ = 0.62, P< 0.001). Small-sized rotifers such as Keratella cochlearis, increased between March and May when Chlorophyceae increased. Both high standing crop of copepods and cladocerans, such as Diaphanosoma brachyrum and Bosmina longirostris occurred between June and September accompanied with the increase of Dinophyceae and Bacillariophyceae. There was no evidence that clear-water phase was caused by zooplankton grazing. The diversity and evenness index of phyto- and/or zooplankton increased with chlorophyll a concentration. These results suggest zooplankton grazing and limiting nutrient deficiency could lead to change of phytoplankton biomass, but not the phytoplankton community in Lake llgam.

Structure and Metallothionein Expression during Rat Liver Regeneration Induced by Partial Hepatectomy (흰쥐 부분 간 절제 후 재생 중인 간 조직의 구조와 metallothionein 분포)

  • Mun, Seung-Hoon;Jeong, Jin-Joo;Lee, Yong-Moon;Shin, Kil-Sang;Kim, Wan-Jong
    • Applied Microscopy
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    • v.38 no.3
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    • pp.175-183
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    • 2008
  • Liver regeneration is a result of highly coordinated proliferation of hepatocytes and non-parenchymal liver cells. At this process, induction of metallothionein (MT), which is low molecular and cysteine rich, has been reported. The present study was carried to find the ultrastructure of hepatocytes and determine the expression of MT in regenerating rat liver after partial hepatectomy. As a result, the remnant liver after PH grew fast from 1 day until 7 days. Various changes were morphologically observed. Disintegration of cell plates and liver lobule appeared shortly after PH. And hepatocytes showed the rapid proliferation, characterized by high nuclear cytoplasmic ratio, weak intercellular junctional complexes, chromatin condensation, increase of ribosomes and mitochondria, and temporary increase of lipid droplets. Finally, remodeling of the liver lobule was completed through the rearrangement of blood vessels and cell plates by 7 days after PH. On histochemistry, immunoreactivity indicating the presence of MT appeared moderately throughout the cytoplasm of control rat hepatocyte. After PH, positive reactions for MT increased at the cytoplasm and the nucleus. These results suggest that the remnant liver cells immediately entered cell proliferation and increase of MT expression after PH. It is thought that MT protein might be associated with transfer of some factors needed to cell division from the cytoplasm to the nucleus for regeneration of the liver after PH.

Quality Characteristics of Doenjang by Aging Period (전통 된장의 숙성 기간에 따른 감각·화학적 품질특성)

  • Ku, Kyung-Hyung;Park, Kyungmin;Kim, Hyun Jung;Kim, Yoonsook;Koo, Minseon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.5
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    • pp.720-728
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    • 2014
  • In order to characterize the quality of Doenjang, fermented Korean soybean paste, subjected to long-term aging, this study performed physico-chemical analyses and sensory evaluation according to aging period (from 1 to 9 years). Regarding the proximate composition of Doenjang according to aging period, moisture, crude protein, crude lipid, crude ash, and salt contents showed little differences among Doenjang samples. Amino-type nitrogen content was 1,046.7 mg% in the 1 year-aged sample, 990.9~996.9 mg% in the 2~5 year-aged samples, and 1,214.1~1,304.8 mg% in the samples fermented more than 5 years. ${\Delta}$E value, reflecting total color differences between the samples, increased according to aging period. Ratios of linoleic and linolenic acids, which are essential fatty acids in soybeans, constituted 55% of total fatty acids, which was the most abundant among all fatty acids. The major free sugar in Doenjang was fructose at a content of 1.6~2.2% in 1~9 year-aged Doenjang. Glycoside form of isoflavones in Meju constituted 77.1%, and the aglycon form constituted 22.9%. However, the glycoside type of isoflavones in soybeans was converted to aglycon type in Doenjang through fermentation and aging. In the sensory evaluation of Doenjang samples, brown color, salt smell, soy sauce flavor, and viscosity all increased according to aging period, whereas sweet flavor, roast smell, beany flavor, salty taste, and acrid taste showed no significant differences. In cluster analysis of the sensory attributes of Doenjang according to aging period, 1 year-aged Doenjang was significantly different between 2 year- and 3~5 year-aged Doenjang.

The Study on the Seasonal Variation of Microbial Community in Kyeonggi Bay, Korea 1. Bacteria and Heterotrophic nanoflagellates (경기만 수역에서 미세생물 군집의 계절적 변동 연구 I. 박테리아와 종속영양 미소 편모류)

  • 양은진;최중기;현정호
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.1
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    • pp.44-57
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    • 2003
  • Seasonal variations of bacterial abundance and production, heterotrophic nanoflagellate (HNF) abundance and HNF ingestion rates on bacteria using FLB together with environmental variables were investigated at intervals of a month in Kyeonggi Bay from December 1991 to November 1998. Bacterial abundance and production ranged from 0.38$\times$10$^{9}$ ~ 3.25$\times$10$^{9}$ cells 1$^{-1}$ (average 1.19$\pm$0.69$\times$10$^{9}$ cells 1$^{-1}$ ) and from 1.51 to 20.4 cells 1$^{-1}$ h$^{-1}$ (average 6.04$\pm$ 1.88$\times$10$^{6}$ cells 1$^{-1}$ h$^{-1}$ ), respectively. Bacterial abundance and production showed no differences at the high tide and low tide, and bacterial abundances were not different with depth, but bacterial production decreased with depth. Seasonal variation of bacterial abundance showed almost similar fluctuation pattern to those of DOC (dissolved organic carbon). HNF abundances ranged from 388 to 4,374 cells ml$^{-1}$ (average 1,344$\pm$130 cells ml$^{-1}$ ), were high in March, April, July and August. HNF abundance showed no difference between the high tide and low tide, and was not different with depth. The ingestion rates of HNF on bacteria were 1.0 to 6.3$\pm$10$^{6}$ bacteria 1$^{-1}$ h$^{-1}$ (average 3.12$\pm$0.55$\times$10$^{6}$ bacteria 1$^{-1}$ h$^{-1}$ ), resulting ingestion rates of HNF removed 19.4 to 141.4 %(average 62.3$\pm$12.0%) of bacterial production. Ingestion rates and grazing pressure of HNF on bacteria showed high correlation with HNF abundance. Although we cannot exactly discussion about seasonal variation of bacteria community in this study area where physical and chemical parameters were very complex, the results indicate that bacterial abundance and production were mainly controlled by resources supply as dissolved organic carbon and chlorophyll-a(bottom-up) except March which bacterial abundance and production uncoupled chlorophyll-a because of low dissolved organic carbon and low temperature, and were controlled by HNF grazing pressure(top-down) in the warm seasons except the winter.

Change of the Vegetation Due to Soyanggang Dam Construction (소양강댐 건설에 따른 주변 식생의 변화)

  • Choi, Ho;Park, Pil-Sun;Kim, Jae-Geun;Suh, Sim-Eun
    • Journal of Wetlands Research
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    • v.12 no.3
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    • pp.1-13
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    • 2010
  • Most of investigations about the effects of dam construction on the surrounding environments have focused mainly on the change of climate conditions and crop production. In order to research the effect of dam construction on the surrounding vegetation, we chose the Soyanggang dam whose storage capacity is the largest in Korea, and was built 33 years ago. We surveyed and analyzed the surrounding vegetation by using quadrat method and measured the soil moisture content among floodplain (FP), 5m above the flood plain (AFP) and control group (CG) which is 3km far from the lake through ridge. The largest value of mean importance percentage of the canopy~understory layer at FP was Salix koreensis (87.9%) and those of AFP and CG was Quercus mongolica (38.9% and 40.4% respectively) and the largest important percentage of the herb layer at FP was Artemisia capillaris (34.2%) and those of AFP and CG was Oplismenus undulatifolius var. undulatifolius (9.4% and 24.6% respectively). The Shannon-Wiener diversity index of shrub~canopy layer at FP (0.26) was lower than AFP (2.34) and CG (2.23) and there was not any significant difference in the herb layer among three groups. The S${\o}$rensen similarity index between FP and AFP, FP and CG was 0, and that of AFP and CG was relatively high. The highest density of tree and subtree with the DBH level of FP was S. koreensis of 5~10cm (240/ha), and that of AFP and CG was Quercus spp. of 15~20cm (400/ha and 466/ha respectively). And the highest density of seedlings of FP was Pinus densiflora (7,040/ha), and that of AFP and CG was Quercus spp. (720/ha and 400/ha respectively). The soil water content of FP (6.28%) was relatively lower than AFP and CG (11.13% and 10.14% respectively; p<.01). These results indicated that construction of Soyanggang dam changed the vegetation of the floodplain, without showing a change in its upland areas.

Analysis of Genetic Characteristics and Probability of Individual Discrimination in Korean Indigenous Chicken Brands by Microsatellite Marker (MS 마커를 이용한 토종닭 브랜드의 유전적 특성 및 개체 식별력 분석)

  • Suh, Sangwon;Cho, Chang-Yeon;Kim, Jae-Hwan;Choi, Seong-Bok;Kim, Young-Sin;Kim, Hyun;Seong, Hwan-Hoo;Lim, Hyun-Tae;Cho, Jae-Hyeon;Ko, Yeoung-Gyu
    • Journal of Animal Science and Technology
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    • v.55 no.3
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    • pp.185-194
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    • 2013
  • Microsatellite markers have been a useful genetic tool in determining diversity, relationships and individual discrimination studies of livestock. The level of genetic diversity, relationships among two Korean indigenous chicken brand populations (Woorimatdag: WR, Hanhyup3: HH) as well as two pure populations (White Leghorn: WL, Rhode Island Red: RIR) were analyzed, based on 26 MS markers. A total of 191 distinct alleles were observed across the four chicken populations, and 47 (24.6%) of these alleles were unique to only one population. The mean $H_{Exp}$ and PIC were estimated as 0.667 and 0.630. Nei's $D_A$ genetic distance and factorial correspondence analysis (FCA) showed that the four populations represented four distinct groups. However, the genetic distance between each Korean indigenous chicken brand (WR, HH) and the pure population (WL, RIR) were threefold that among the WR and HH. For the STRUCTURE analyses, the most appropriate number of clusters for modeling the data was determined to be three. The expected probabilities of identity among genotypes of random individuals (PI) were calculated as $1.17{\times}10^{-49}$ (All 26 markers) and $1.14{\times}10^{-15}$, $7.33{\times}10^{-20}$ (9, 12 with the highest PI value, respectively). The results indicated that the brand chicken breed traceability system employing the own highest PI value 9 to 12 markers, and might be applicable to individual identification of Korean indigenous chicken brand.

Characterization and Control of Vascellum curtisii (Berkeley)Kreisel Causing the Fairy Ring Arcs in the Golf Course in Korea (골프코스에서 페어리링의 원인이되는 Vascellum curtisii의 특징과 방계)

  • Choi, Dae-Hong;Lee, Jung-Han;Kim, Hee-Kyu
    • Asian Journal of Turfgrass Science
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    • v.22 no.2
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    • pp.171-178
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    • 2008
  • We have found the clusters of tiny spiny puffball-like mushrooms growing gregariously in fairy ring (arcs) rimmed by a zone of darker green grass in the golf courses. Macroscopic as well as microscopic characters were examined for the morphology of fruiting body. Exoperidium is thin and densely spiny with minute fibrillae at early stage. The connivent spines were soft and quite persistent. In age, the fibrillae scrumble away with a powdery coating, which leaves white endoperidium becoming pale brown. It's interior was white and fleshy at first, but turns into an olive-colored dust as the gleba, the spore-producing tissue, develops to maturity and loaded with olive-brown spore mass. Then, distinct apical pore developed on the endoperidium. Rudimentary subgleba(sterile base) was narrow, chambered, delineated from the gleba by a membrane in young material. These characters suggested this fungus is a Vascellum, a member of the family Lycoperdaceae. The shapes of the spores were globose, echinulate, $3{\sim}3.5{\mu}m$ in diameter, thick-walled, and olive brown. Capillitial threads were $8-9{\mu}m$ wide, mostly colorless in KOH solution and thin-walled, which designated as "paracapillitium". This is an another character that distinguishes this mushroom from Lycoperdon spp. The spines developed on exoperidium were characteristically connivent; their apices joined together in a point, leaving a space below, which gives the appearance of vault to each group of usually 5 to 6 fibrillae. Based on the above characters, this fungus is identified as Vascellum curtisii (Berkeley). The characters distinguishable this from Lycoperdon pulcherrimum, and Vascellum pretense are discussed in detail. Control trial was also attempted. Strong vertical raking(SVR) followed by applying 500x detergent solution (Spark, Aekyung Co. Seoul) resulted in excellent control over any other treatments. In this plot, fruiting body was not developed throughout the end of mushroom growing season.

Comparison of Plant Community Structures in Cut and Uncut Areas at Burned Area of Mt. Gumo-san (금오산(金烏山)의 산화지(山火地)에서 벌목지(伐木地)와 비벌목지(非伐木地)의 식물(植物) 군집구조(群集構造) 비교(比較))

  • Che, Sang-Hoon;Kim, Woen
    • Journal of Korean Society of Forest Science
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    • v.86 no.4
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    • pp.509-520
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    • 1997
  • This is a report on the early vegetation, plant community structure, and secondary succession of cut and uncut sites of burned areas in Mt. Gumo-sun. The forest fire occurred on April, 1994 and the pine forest and its floor vegetation were burned down. The investigation was carried out from April, 1995 to October, 1996. The results are summarized as follows : The floristic composition of cut and uncut sites of burned area and unburned area were composed of 32, 36, and 34 kinds of vascular plants respectively. The biological spectra showed the $H(G)-D_1-R_5-e$ type, $H(M)-D_1-R_5-e$ and $M(N)-D_1-R_5-e$ in cut, uncut, and unburned site respectively. The dominant species based on $SDR_3$ of the cut site were Miscanthus sinensis var. purpurascens(100.00). Caret humilis(52.27), Quercus serrata(51.19) and Lysimachia clethroides(39.40), however, in the uncut sites the dominant species were Quercus acutissima(56.91), Pinus densiflora(26.83) in the tree layer, Quercus serrata(50.43), Lindera glauca(40.51), Lespedeza bicolor(37.85) in the shrub layer, and Miscanthus sinensis var. purpurascens(72.27), Pteridium aquilium var. latiusculum(60.92), Carex humilis(63.63) in the herb layer. Pinus densiflora(99.88), Miscanthus sinensis var. purpurascens(82.74), Quercus serrata(77.47) and Carex humilis(74.02) were dominant in the unburned site. The species diversity(H) and evenness index(e) were 1.05, 0.70 and 1.32, 0.85 in the cut and uncut site, respectively and 0.22, 0.63 in the unburned site. Dominance index(C) was 0.15, 0.06 and 0.96 in the cut, uncut site and unburned site, respectively. Degree of succession(DS) was 345.19, 747.47 and 674.34 in cut, uncut and unburned site, respectively. The index of similarity(CCs) was 0.66 between cut and uncut sites, 0.50 between unburned and cut sites and 0.61 between unburned and uncut sites. The amount of exchangeable sodium, calcium, magnesium and soil pH were increased, but the amount of organic matter, available phosphous, total nitrogen, total carbon and exchangeable potassium were decreased in cut site after fire.

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Study on the Influence of Waterbirds Distribution According to the Restoration of Intertidal Zone (조간대 복원이 수조류의 분포에 미치는 영향)

  • Park, Chi-Young;Shin, Man-Seok;Kim, Ho-Joon;Paek, Woon-Kee;Lee, Joon-Woo
    • Korean Journal of Environment and Ecology
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    • v.30 no.5
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    • pp.837-847
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    • 2016
  • In this study, the bird fauna of Sihwa Lake in Gyeonggi Province were investigated from 2009 to 2014 to identify the effect of the restored intertidal zone on bird community. Based upon the analysis of the habitat status and distribution change of water birds in the intertidal zone, the following conclusions were obtained. The number of water bird individuals increased by approx. 40 thousand after the restoration. Most of water bird species increased, but diving ducks decreased. Analysis of the number of individuals of water birds before and after the intertidal zone restoration showed that there was a significant increase in the number of shorebird individuals. There is a habitat use pattern change in the southern tideland intertidal zone that includes the largest area of widened intertidal zone and the inland waters of the intertidal zone. This is related to the change of benthic ecosystem which is the food source of birds. The benthic ecosystem in restoring Sihwa Lake has been stabilized with a decrease in opportunistic species and consistent increase in equilibrium species that are sensitive to pollution. As a result, the environmental conditions for shorebirds have been improved. The restoration of intertidal zone affected the habitat use and distribution patterns of water birds in a short period of time. Compared to before restoration, all the water birds now use the wide area of the intertidal zone with no partiality and it is confirmed that the restoration greatly affected the distribution of shorebirds and swan & geese. This study was conduced to identify the effects of an artificial restoration of intertidal zone due to tidal power generation of bird species. In terms of maintaining and improving biodiversity, the intertidal zone restoration was recognized to be important and still shows positive results. This study is expected to provide a direction when an alternative is required to maintain and improve biodiversity in a similar situation in the future.

Effects of the Aquatic Vascular Plants on the Lake Ecosystem in the Upper Stream Wetlands of the Namgang-Dam (남강댐 상류 습지에서 수생관속식물이 호소생태계에 미치는 영향)

  • Oh, Kyung-hwan;Lee, Jeong-Hwan;Kim, Cheol-Soo;Son, Sung-Gon;Lee, Pal-Hong
    • Journal of Wetlands Research
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    • v.1 no.1
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    • pp.29-44
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    • 1999
  • Vegetation structure and distribution of the vascular hydrophytes and hygrophytes, and the growth pattern, standing crop and amounts of nutrient uptake by Salix species were investigated in the upper stream wetlands of the Namgang-Dam, Chinju-city, Gyeongsangnam-do, Korea from April to November in 1997. The flora was composed of 43 hydrophytes and 241 hygrophytes, or total 284 vascular plants. The life forms of the hydrophytes were classified as 27 kinds of emergent plants, 4 floating-leaved plants, 3 free-floating plants, and 9 submersed plants. In the herb layer, the dominant species was Persicaria hyciropiper, and the ranges of the species diversity indices (H'), equitabilities, (J') and community similarity indices (CCs) were 1.59~1.89, 0.87~0.96, and 0.35~0.83, respectively. In the shrub and subtree layers, 17 kinds of Salix species were supposed to the pioneer plants at the early stage of the succession. The number of branches per main stem of Salix species was 5.0. The DBH class-frequency histograms of Salix species were the reverse J type, and the natural regeneration of the Salix community was expected. Basal area of Salix species per square meter was $24.87cm^2$. Volume of Salix species per square meter was $12,008cm^3$ and total phytomass of the Salix species was estimated as 12,894 ton. Biomass distribution of Salix species in the stem, the branch and twig, and the leaf was 64.1%, 28.1%, and 7.8%, respectively. The amounts of nitrogen and phosphorus absorbed by Salix species were 68,022 and 19,823 kg. It was recommended that application and conservation of the wetland and other counterplans are indispensable to reduce the adverse effects of water pollution and to preserve the wetland ecosystem.

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