• Korean Journal of Weed Science
• /
• v.7 no.1
• /
• pp.19-28
• /
• 1987

These experiments were conducted to recognize the germination change in relation to storage period, using 25 kinds of field-collected weed seeds stored under the dry and low temperature ($4{\pm}1^{\circ}C$), and to establish a simple germination induction methods in some kinds of them. No or weak dormancy was observed in Lepidium virginicum, Rumex japonicus, Cirsium japonicum var. ussuriense, Aeschnomene indica and Ludwigia prostrate. The germination of Digltaria sanguinalis, Portulaca oleracea, Amaranthus spp., Echinochloa crux-galli, Polygonum persicaria, Cyperus iria, and Aeschynnmene indica was enhanced by $H_2SO_4$ treatment for 20, 2, 2, 50, 40, 3 or 5 minutes, respectively. Favorable germination in Polygonum persicaria and Echinochloa crus-galli was obtained by soaking in water for 25 days and 50 days, respectively. Moisture treatment for 21 days and 14 days caused the enhancement of germination in Cyperus difformis and Aneilema keisak, respectively. Combination of various treatments in Cyperus iria was most effective when the seeds treated with $H_2SO_4$ for 3 minutes incubated in alternating temp. after soaking in 0.2% $KNO_3$, but their effect on Digitaria sanguinnlis was little.

• Research in Plant Disease
• /
• v.13 no.3
• /
• pp.157-163
• /
• 2007

Hot water treatment that is the most appropriate seed disinfection method for organic vegetable farming was evaluated in this study. Among the leafy vegetable seeds lettuce that was the most sensitive to hot water was suitable to treat at $45^{\circ}C$ for 25 min, while Chinese cabbage and radish seeds were optimally treated at $50^{\circ}C$ for 25 min. The treatments resulted in similar or higher seed germination rate than non-treated seeds and promoted plant growth. In addition, fungi such as Alternaria, Aspergillus, Penicillium, or Mucor grown on the seeds were suppressed over 90% and the bacterial growth on lettuce seeds reduced 98.5% by the treatment. Among the fruit vegetable seeds pumpkin that was vulnerable to hot water was suitable to treat at $50^{\circ}C$ for 15 min, while cucumber and hot pepper seeds revealed optimum treatment at $50^{\circ}C$ for 25 min as chinese cabbage and radish. The treatment also showed similar or higher seed germination rate and growth than non-treated seeds. Furthermore, fungi such as Rhizopus, Aspergillus, Penicillium or Mucor grown on the seeds reduced from 72.0% to 95.4%. The bacterial growth on cucumber and red pepper seeds was suppressed from 65.5% to 86.0% by the treatment. Results indicated that the hot water treatment is practical for disinfection of organic vegetable seeds and the optimum temperature and soaking time varied among the seeds.

• Korean Journal of Organic Agriculture
• /
• v.15 no.1
• /
• pp.109-121
• /
• 2007

For developing standard method for diease, pest and weed control in environmental friendly 'Ssam' vegetable cultivation, this study was carried out to investigating agriculture material use in organic agriculture and no pesticide cultivation for lettuce, kale, leafy perilla and korean cabbage. The 28.6% of investigated farmer carried out seed sterilization by seed selection with salt solution and soaking in chitosan that not validated. For raising seedling periods, the 55.6% of farmer did not use environmental-friendly agriculture material for, diease control and the 50% of farmer used one time for. pest control. Therefore, the control of disease and pest could be achieved with one or two times use of environmental-friendly agriculture material. Seed sterilization was carried out by soil solar sterilization, one time per year in 71.4% of farmer. Weed was controled by black PE film for weed germination of furrow in many farmer, by man-power weeding for weed of ridge in 85% of farmer and by machine weeding and mulching in some farmer. During cultivation period, the major pest were Aphis gossypii in lettuce, Plutella xylostella in kale, Plutella xylostella and Phyllotreta striolata (Fabricius) in korean cabbage and Pyrausta panopealis (Walke) in feat perilla. The many farmers used environ-mental-friendly agriculture material for control of pest over 10 times for spring season, and more used sold materials in market than home-made materials. In result, it needs to develop standardized method and validate cultivation methods for control of disease and pest, and seed sterilization treatment environmental-friendly 'Ssam' vegetable.

• Korean Journal of Organic Agriculture
• /
• v.18 no.1
• /
• pp.93-104
• /
• 2010

This study was conducted screening of the population densities of fungal pathogens and insect and observed the disease symptoms on the organic sweet corn seed producing field from 2008 to 2009. The dissemination spores, Alternaria sp., Cladosporium sp., Helminthosporium sp., Pyricularia sp., Collectotrichum sp., and Bipolaris sp., were detected and the three fungal spores from the front were observed for whole growth stage. Seed and seedling diseases were shown as the rotted seed and damping-off seedling caused by Penicillium sp. and Rhizoctonia sp.. The larva of Black cutworm cut down the root crown of seedlings. The damaged plants were ranged from 14% to 16%. On the Oriental corn borer, the population densities and the percentage of damaged plants were showed a low difference between two sweet corn varieties. The population densities of Oriental corn borer were scored as from 3.5 to 20.5 in 2008 and from 0.5 to 6 in 2009. Also the percentage of damaged plants were significantly increased until harvesting stage and was recorded from 7.5% to 21% in 2008 and from 1% to 46% in 2009. On the Corn leaf blight, the percentage of diseased plant were scored from 7% to 34% in 2008. The first occurrence of date was after June 18, and the percentage of diseased plant was continuously increased after August 21 and the values of diseased plants was ranged from 56% to 69% in 2009. On common smut, the percentage of diseased plant was recorded from 5% to 15% in 2008, and the first occurrence date were delayed as 17 days (July 17) and were showed less than 8% of diseased plants in 2009. Corn Southern Leaf spot was scored as average 11% at early stage and showed high score as 62% at September 19 in 2008. In 2009, the first occurrence date were advanced about 20 days (after June 8), and continuously increased up to 86% in 2009.

• Korean Journal of Environmental Agriculture
• /
• v.26 no.3
• /
• pp.217-222
• /
• 2007

To investigate effects of inorganic $(GeO_2)$ and organic (Ge-132) germanium (Ge) on seed germination and on early stage growth of plane and the uptake characteristics, various concentrations (0, 10, 25, 50, 100 mg $L^{-1}$) of Ge to popular vegetables such as leaf mustard, chinese cabbage and pak-choi, respectively, were treated. On seed germination, no significant effect was observed in both inorganic and organic Ge treatments except 100 mg $L^{-1}$ treatment of inorganic Ge. Exogenous inorganic Ge ($10{\sim}100$ mg $L^{-1}$ treatments significantly inhibited the early root elongation growth of all plants. However, slight enhancement of early shoot elongation was detected in low concentrations (10 and 25 mg $L^{-1}$) of Ge in the leaf mustard and chinese cabbage plants. Organic Ge treatments significantly stimulated the 개ot and shoot growth at the 10, 25 and 50 mg $L^{-1}$ treatments. Ge was accumulated linearly in the vegetables as both inorganic and organic Ge concentrations were increased. Interestingly, total contents of Ge in plants with Ge-132 treatments were $2\sim4.5$ times more than those with inorganic Ge treatments in all concentrations. At 25 mg $L^{-1}$ treatment of Ge, contents of Ge in vegetables are following: in leaf mustard, inorganic Ge: 0.37 mg $g^{-1}dw$ and organic Ge: 1.47 mg $g^{-1}dw;$ in the chinese cabbage, inorganic Ge: 0.4 mg $g^{-1}dw$ and organic Ge: 0.86 mg $g^{-1}dw;$ in the pak-choi, inorganic Ge: 0.33 mg $g^{-1}dw$ and organic Ge: 0.70 mg $g^{-1}dw$, respectively. These results showed organic Ge is much better on early stage seedling growth and on germanium accumulation of vegetables than inorganic Ge.

• 한국약용작물학회:학술대회논문집
• /
• 2011.04a
• /
• pp.154-155
• /
• 2011

• Weed & Turfgrass Science
• /
• v.1 no.4
• /
• pp.1-5
• /
• 2012

A simple monitoring method was designed to evaluate seed bank in a upper soil (0 to 30 cm depth), which was observed for the pattern of vertical distribution of weed in the soil under tillage or no-tillage condition. The field experiment was established at an organic corn field located in Hwacheon in Kangwon-do from 2010 to 2011. Undistributed linear soil samples were taken using non-destructive soil sampler from 0 to 30 cm depth at the tillage or no-tillage soils. Weed seed distribution in the linear soil samples was estimated by counting the number of weed germinated according to the soil depth. Under tillage condition, the weed seeds were more evenly distributed from 0 to 30 cm depth, with being 75% of weed seeds located in 0 to 15 cm depth compared to the no-tillage condition. Soil samples taken by no-tillage condition had 85% of weed seeds within 15 cm of soil depth, with being 93% of weed seeds from 0 to 20 cm depth. The number of weeds or the number of weed species were three times higher for tillage soil compared to no-tillage soil, and the major dominant weed species were observed for annual plants, such as Echinochloa crus-gall, Mollugo pentaphylla, and Digitaria ciliaris.

• Research in Plant Disease
• /
• v.29 no.3
• /
• pp.268-275
• /
• 2023

This study was conducted to confirm the utilization of Pleurospermum camtschaticum root extract as an organic agricultural material. Antioxidant activity of P. camtschaticum root extract, closely related to antibacterial activity, increased in a dose-dependent manner. In mycelial growth inhibitory activity, 100% P. camtschaticum root extract supressed over 70% for Colletotrichum coccodes and over 68% for Colletotrichum dematium. In the pepper fruit anthracnose development test, the size of the lesion decreased in a dose-dependent manner, which showed the same tendency as the previous results in inhibitory activity on mycelial growth. In the pepper seed germination and red pepper growth promotion test of P. camtschaticum root extract, oposite results was confirmed. The lower the concentration, the more the seed germination and growth promotion effects were shown. The phenol content of pepper leaves was also measured after pepper growth promotion test have been completed. The phenol content related to antibacterial activity increased in all treated groups compared to the untreated group. Therefore, the results of this study showed the possibility of development as an organic material.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.38 no.5
• /
• pp.442-448
• /
• 1993

The aims of the work were to examme the variability induced by EMS (ethyl methanesulfonate) mutagenesis of soybean plants, and to isolate mutants altered in nodulation and other growth characters. Seeds of two soybean cultivars, ‘Hwanggeumkong’ and ‘Baegunkong’ were treated with 30 and 50mM EMS(pH 7.0) for 6 hours and were planted directly in the field. Field emergency of$M_1 seed was averaged to be 61.0%, and frequency of plants with chlorophyll-deficient sectors of the first trifoliolate is about 0.7%. Regardless of varieties and does of EMS, $M_1 plant injury at harvest was present in plant height, pod and seed number per plant when compared to those of original-type soybean plants. The $M_2 variability of nodulation process induced by EMS treatment was found to be narrower than that of shoot dry weight. On the basis of the occurrence of chlorophyll-deficient plants, mutated cell frequency within $M_1 seed ranged from 5.3% to 84.2%, suggesting that mutation frequency on the $M_1 seed induced by EMS occurred partly and randomly regardless of varieties and doses of EMS. The putative mutant, which had more nodulation than original-type plant, was short in plant height. Sparse-nodulating soybean mutant was lower in leaf chlorophyll content and showed reduced growth.

• Korean Journal of Weed Science
• /
• v.16 no.2
• /
• pp.114-121
• /
• 1996

This study was conducted to investigate seed germination, seedling growth, and responses to herbicides of Bidens tripartita L. When the field-collected seeds were stored under a dry-room temperature, dry-low temperature, wet-low temperature, or dry-high temperature condition, no seeds were germinated in a growth chamber with 14 hr photoperiod up to 35 days after the storage. Exceptionally, however, some seeds stored under a wet-room temperature condition were germinated after 25 days of the storage. This might be due to the fact that the seed coats were damaged by fungi which developed during the storage. Seeds stored under a wet-low temperature condition (stratification) began to be germinated after 3 months of the storage and the germination rate increased with a prolonged stratification. Almost all seeds were germinated after 9 months of the stratification. These results suggest that the dormancy of B. tripartita L. seeds relate to the seed coat and thus several attempts were made to induce seed germination through damaging or weakening the seed coat. Freezing($-20^{\circ}C$), drying($100^{\circ}C$), or swelling($40^{\circ}C$) of the seeds was not effective to induce the germination. Treatments of concentrated sulfuric acid, $KNO_3$, or gibberellin to the seeds had no effect on inducing the germination. However, ethrel had a stimulatory effect on the germination of the seeds with an optimum concentration of 250ppm. A seed cutting was also effective to induce the germination, but seedlings from the seeds had cutted cotyledons. Germination of the stratified seeds varied with the temperature condition to which they were subjected, but not with light. The germination rate was the highest at 35 - $40^{\circ}C$. Although the seeds were not able to germinate under a submerged condition, seedlings after 2-leaf-stage exhibited better growth under a submerged or a subirrigated condition than under an upland condition. Among the herbicides tested, pyrazosulfuton-ethyl, linuron, and bentazone were found to be effective for controlling B. tripartita L., having more herbicidal effect with an earlier application.