• Title/Summary/Keyword: 요소분해효소 생산 세균

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Effects of Culture Environments on Alkaline Protease Biosynthesis in Streptomyces sp. (Streptomyces속 세균에서 호염기성 단백질 분해효소 생합성에 미치는 배양환경의 영향)

  • 노용택;김종웅;이계준
    • Korean Journal of Microbiology
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    • v.28 no.2
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    • pp.162-168
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    • 1990
  • The aims of the present study were to evaluate the effects of culture conditions on the biosynthesis of extra-cellular alkaline protease in Streptomyces sp. The formation of aerial mycelia and spores were compared with the protease production in order to know the relations between the alkaline protease and the cell differentiation. As results, it was found that substrate concentration was very critical to regulate the formation of the protease, aerial mycelia, and spores, which were resulted from the changes of culture pH to acid. When the culture pH was adjusted with phosphate buffer from pH 6 to pH 9, the alkaline protease production was increased as the culture pH increased whereas aerial mycelia and spore formation were reversely related to the culture pH. Therefore, it was thought that the culture pH was an important factor to regulate the alkaline protease synthesis.

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A Case of Struvite Urolithiasis in a One-month-old Korean Sapsal Dog (1개월령 삽살개에서 발생한 Struvite요도결석 1례)

  • Oh, Won-Seok;Oh, Tae-Ho
    • Journal of Veterinary Clinics
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    • v.27 no.4
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    • pp.453-456
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    • 2010
  • A one-month-old, 0.9 kg BW, male Korean Sapsal dog with anorexia, anuria, ataxia and depression was referred. On physical examination, the dog was showed tachycardia, hypothermia and pale mucous membrane. Hematologic values showed leukocytosis and mild anemia. Serum chemistry profile results revealed increased BUN (57.3 mg/dl) and $NH_3$ ($584\;{\mu}g/dl$), decreased albumin, sodium and potassium. Urinalysis showed hematuria, proteinuria, glucosuria, bacteriuria (Staphylococcus spp.) and magnesium ammonium phosphate crystalluria. On radiographic finding, urinary bladder was enlarged and 2 mm diameter radiopaque urolith was showed between os penis and prescrotal region. Urolithiasis was surgically corrected by urethrostomy.

Effect of Non-Starch Carbohydrase Supplementation on Productivity, Blood Parameters, and Cecal Microorganisms in Broiler Chickens (비전분성 탄수화물 분해효소 혼합급여가 육계의 생산성, 혈중 대사물질, 및 장내미생물의 변화에 미치는 영향)

  • Sun, Sang Soo;Park, Cheol Ju
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.12
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    • pp.437-443
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    • 2019
  • This study examined the productivity, blood characteristics, and cecal microorganisms with the addition of mixed enzymes in broiler chickens. Three hundred chickens (Ross 308) were assigned randomly to five treatment groups (control, 0.5 MXG, 1.0 MXG, 2.0 MXG, and 1.0 G) with three replications. Based on the results, the weight, feed intake, feed efficiency, and daily gain increased slightly by the treatment assignment. Carcass, stomach, and heart weights increased slightly in all treatment groups compared to the control. On the other hand, liver weight was significantly low by enzyme addition compared to the control group (p<0.05). The total number of appendix bacteria decreased significantly in all treatment groups compared to the control group (p<0.05). The total protein, blood urea nitrogen (BUN), and glucose level did not differ after treatment. On the other hand, the IgG level was significantly higher in the 1.0 MXG and 2.0 MXG groups than in the control group (p<0.05). In conclusion, the addition of a mixed enzyme (MXG) will improve the feed efficiency and IgG, as well as reduce the liver weight and total bacteria in broiler chickens.