• Title/Summary/Keyword: 온도(temperature)

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Production and Inhibition of Cellulolytic and Pectolytic Enzymes by Cylindrocarpon destructans(Zins.) Scholten Causing Root Rot of Ginseng (인삼뿌리썩음병균, Cylindrocarpon destructans에 의한 섬유소분해효소 및 펙틴질분해효소의 분필 및 억제)

  • Lee Jin Woo;Chung Hoo Sup
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.1-10
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    • 1974
  • The activities of pectolytic and cellulolytic enzymes produced from slices of ginseng root infected with Cylindrocarpon destructains(Zins.) Scholtern were proportional to each concentration and reaction time. Activities of cellulase(Cx), endo-polygalacturonase(endo-PG), endo-polymethylg-alacturonase(endo-PMG), exo-polygalacturonase(exe-PG), and exe-polymethylgalacturonase(exo-PMG) were maximum on the 4th day after inoculation. No endo-PG and endo-PMG were detected at the first and second days, while exo-PG exo-PMG were active. On the 6th day, all pectic enzymes were completely lost, whereas Cx remained at a high concentration. pH optima of Cx, endo-PG, endo-PMG, exo-PG, and exo-PMG were 6.0, 5.5, 8.0, 7.0 to 7.5, and 8.5, respectively. Temperature optima of Cx, endo-PG, endo-PMG exo-PG, and exo-PMG were $66^{\circ}C\;53^{\circ}C\;41^{\circ}C\;37^{\circ}C\;and\;40^{\circ}C$, respectively. Cx was only inhibited by $0.05M\; Hg^{++}$ among 16 ions tested. Inhibitory effects of ions on pectolytic enzymes varied, however$M Fe^{+++}\;and\;0.05M\;Al^{+++}$ were the best in general. Among 8 fungicides, none of them inhibited all the enzymes studied at $0.1\%$, active ingredients. Exo-PG were highly inhibited by all of the fungicides, of which difolatan was the most inhibitory to all the pectic enzymes. $Ca^{++}\; at\; 0.02M\; and\;Fe^{+++}\;at\;0.02M$ completely inhibited all the pectolytic enzymes, and Cx was inhibited $30\%$ and $70\%$ at the same concentration, respectively Formalin almost inhibited exo-PG and exe-PMG at $0.8\%$ but not the other enzymes especially Cx. Difolatan at $0.8\%$ inhibited all the enzymes concerned above $80\%$. The cellulolytic and pectolytic enzymes of C. destructans must be closely associated with the ginseng root rot and should be inhibited to control the disease effectively.

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Species Identification of Noctuid Potential Pests of Soybean and Maize, and Estimation of Their Annual Adult Emergence in Suwon, Korea (수원지방에서 콩과 옥수수 가해 밤나방과의 잠재해충에 대한 종 동정과 연중 성충 발생 추정)

  • Jung, Jin Kyo;Kim, Eun Young;Kim, I Hyeon;Seo, Bo Yoon
    • Korean journal of applied entomology
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    • v.59 no.2
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    • pp.93-107
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    • 2020
  • Adults of seven noctuid potential pests (Spodoptera frugiperda, S. litura, S. exigua, Ctenoplusia agnata, Mythimna loreyi, Athetis dissimilis, and A. lepigone) of soybean and maize in Suwon, Korea were identified by their morphological characteristics in the wing pattern and male genitalia and partial mitochondrial DNA sequences of cytochrome c oxidase subunit 1 gene. The generation number of adults that emerge annually in six species (except A. lepigone) was estimated from the data on density fluctuations of adults caught in sex pheromone traps in 2019 and the forecasted data using temperature-associated development and reproduction models for those species. S. frugiperda adults were caught from July 27th to October 31st in 2019, and hence were initially estimated to emerge three times per year. But, it was finally expected that S. frugiperda adults could possibly emerge a total of four times per year in Suwon, considering larval emergence observed during mid- and late June in other areas. Adult emergence of S. litura, S. exigua, C. agnata, and M. loreyi in 2019 was observed from May 29th to November 6th, from May 14th to November 6th, from May 26th to October 25th, and from May 31st to November 23rd, respectively. Annual adult emergence of these four species was estimated as at least four times. Adults of A. dissimilis were caught from May 26th to September 11th in 2019, and adult emergence was estimated at only twice per annum. It was postulated that the first adult populations of five species except the two Athetis species were probably migrated from other areas.

Effects of 1 keV $Ar^+$ ion irradiation on Au films on glass (1 keV $Ar^+$ 이온의 조사가 유리기판위의 금 박막의 미치는 영향)

  • Jang, H. G.;Kim, H. S.;Han, S.;Choi, W. K.;Koh, S. K.;Jung, H. J.
    • Journal of the Korean Vacuum Society
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    • v.5 no.4
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    • pp.371-376
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    • 1996
  • Au films with a thickness around 1600 $\AA$ were deposited onto glass at room temperature by ion beam sputtering with a 5 cm cold-hollow ion gun at pressure $1\times 10^{-6}-1\times 10^{-5}$ Torr. Irradiation of the Au deposited samples was carried out at pressure of $7\times 10^{-6}$ Torr. For the sputter depositions, $Ar^+$ ion energy was 1 keV, and the current density at the substrate surface was 15 $\mu$A/$\textrm{cm}^2$. Effects of 1 keV $Ar^+$ ion dose($I_d$) between $1\times 10^{16}\; and\;2\times 10^{17}\;Ar^+\textrm{cm}^{-2}$on properties such as crystallinity, surface roughness and adhesion, etc. of the films have been investigated. The Au films sputtered by $Ar^+$ ion beam had only (111) plane and the X-ray intensity of the films decreased with increase of $I_d$. The thickness of Au films reduced with Id. $R_{ms}$ surface roughness of the films increased from 16 $\AA$ at as-deposited to 1118 $\AA$ at ion dose= $2\times 10^{17}\;Ar^+\textrm{cm}^{-2}$. Adhesion of Au film on sputtered at $I_d$= $2\times 10^{17}\;Ar^+\textrm{cm}^{-2}$ was 9 times greater than that of Au film with untreated, as determined by a scratch test.

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Effect of Irradiation, Packaging and Storage on the Oxidation of Cholesterol and Lipid in Pork Longissimus Meat (돈육 등심의 콜레스테롤 및 지질 산화에 전자선 조사, 포장 및 저장기간이 미치는 영향)

  • 신택순;이정일
    • Food Science of Animal Resources
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    • v.22 no.2
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    • pp.137-144
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    • 2002
  • Pork loins that retailed in market were used as experimental samples. Some pork samples in raw state were packaged with PVDC in either aerobic or vacuum condition. The other pork samples were cooked until core temperature reached at 70$\^{C}$ and then packaged immediately in the same way with the raw samples. After these samples were irradiated by electron beam 6 kGy, the samples were stored in a refrigerator (2∼4$\^{C}$). Identification and quantification of cholesterol oxides were performed at 0 and 7 days. The results were following. During the early stage of storage, cholesterol oxides were not produced from the raw meat samples, but with the passage of storage time,7 $\alpha$-hydroxycholesterol, 7$\beta$-hydroxycholesterol, 7-ketocholesterol, 20 $\alpha$-hydroxycholesterol, $\beta$-epoxide and $\alpha$-epoxide, which were not produced during the early stage of storage, were produced. The production of cholesterol and lipid oxidation products were significantly higher (P<0.05) in the meats with aerobic packaging than those with vacuum packaging, Cooked meat after irradiation showed 7 $\alpha$-hydroxycholesterol, 7 $\beta$-hydroxycholesterol, $\alpha$-epoxide and cholestanetriol on the 7th day of storage, although those chemicals were not produced during the early stage of storage. Production of cholesterol oxides was significantly increased (P<0.05) with the passage of storage time for all treatments, and showed significantly lower value (P<0.05) with the vacuum packaging than these for aerobic packaging. Species of cholesterol oxides from irradiated meat after cooking were similar to those from cooked meat after irradiation. Collectively, it was found that the production of cholesterol oxides was more easily affected by packaging condition than irradiation.

Annual Variations of Litterfall Production in a Broadleaved Deciduous Forest at the Mt. Keumsan LTER Site (금산 장기생태연구 조사지 낙엽활엽수림 낙엽낙지량의 연변동)

  • Kim, Choonsig;Lim, Jong Hwan;Lee, Im Kyun;Park, Byung Bae;Chun, Jung Hwa
    • Journal of Korean Society of Forest Science
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    • v.102 no.2
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    • pp.210-215
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    • 2013
  • Litterfall production represents a major contribution of carbon and nutrient cycling in forest ecosystems. This study was carried out to determine the litterfall production in a broadleaved deciduous forest at the Mt. Keumsan Long Term Ecological Research (LTER) site, Southern Korea. Littefall was collected monthly or bimonthly from the site for 7 years from 2004 to2010. Leaf and reproductive (catkins) litters showed a seasonal variation, but litters of needle, branch, and barks were not changed across the seasons. Annual leaf litter of Quercus serrata and Carpinus laxiflora were significantly different (p<0.05) but that of C. cordata, Chamaecyparis obtusa, and Pinus thunbergii was not significantly changed for 7 years (p>0.05). Annual average litterfall production was 5,223 kg/ha, but annual variations were very large with minimum of 4,110 kg/ha/yr in 2004 and maximum of 6,002 kg/ha/yr in 2007. Total litterfall comprised of 2,323 kg/ha/yr in Q. serrata, 442 kg/ha/yr in C. laxiflora, 157 kg/ha/yr in C. cordata, 131 kg/ha/yr in Acer pseudosieboldianum, 390 kg/ha/yr in other deciduous tree species, 74 kg/ha/yr in P. thunbergii, 37 kg/ha/yr in C. obtusa, 672 kg/ha/yr in branches, 515 kg/ha/yr in miscellaneous, 448 kg/ha/yr in reproductive parts, and 54 kg/ha/yr in barks. respectively. The results indicate that litterfall production of the Mt. Keumsan LTER site was yearly fructurated with the positive linear relationship between leaf or total litterfall and annual mean temperature if no disturbance such as a typoon, and was lower than that of other Korean LTER sites.

Changes in Quality of Boiled Neverita didyma as a Function of Autoclaving Conditions (큰구슬 우렁이 (Neverita didyma) 보일드 통조림의 가열살균(加熱殺菌) 조건(條件)에 따른 품질변화(品質變化))

  • Lee, Keun-Woo;Ryu, Hong-Soo;Joo, Hyen-Kyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.3
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    • pp.231-237
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    • 1984
  • Changes in available lysine and water soluble proteins were checked to obtain the desirable condition of processing for boiled Neverita didyma under the various autoclaving temperatures and times. It was also carried out to assess the affect of heat treatments upon the solubility of coagulable substances in boiled products. In order to evaluate the nutritional quality of processed Neverita didyma under the highest available lysine retention and without bacteria, it was studied the value of pepsin-pancreatin digest residue index in comparison with unprocessed (raw) material. The crude protein content of raw material was higher than that of the other mollusks, while the content of crude fat was showing lower value. It was noticed that the higher contents of coagulable substances and water soluble proteins in processed Neverita didyma were obtained under the conditions, retort temperature at $120^{\circ}C$ above, and 40-60 min. sterilization. After the pannel test, the coagulable substances were not found below the level of $0.40{\pm}0.03$ gram nitrogen/ml of juice. The remarkable retention of available lysine (FDNB-reactive lysine) was resulted in the conditions of autoclaving at $115^{\circ}C$ for 40 minutes in boiled Neverita didyma products, and that processing condition was coincide with the condition of commercial sterilization for boiled top shell. It was revealed that the boiled meat of Neverita didyma showed the higher PPDRI(65.85) than that of raw material (63.16).

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Influences of Environmental Gradients on the Patterns of Vegetation Structure and Tree Age Distribution in the East Side of Cascade Range, Washington, USA (워싱턴주(州) 케스케이드산맥(山脈) 동(東)쪽 산림(山林)에서 환경구배(環境勾配)가 식생구조(植生構造)와 연령분포(年齡分布)에 미치는 영향(影響))

  • Woo, Su Young;Lee, Kyung Joon;Lee, Sang Don
    • Journal of Korean Society of Forest Science
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    • v.85 no.1
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    • pp.107-119
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    • 1996
  • To understand vegetation changes along environmental gradients in the natural forests in the east side of the Cascade Range in Washington state, USA, line transects were used to sample six different forest environments in the Wenatchee National Forest in the north-facing and south-facing sites at 975, 1280 and 1700m elevation. Data were analyzed using ordination by detranded correspondence analysis. Pseudotsuga menziesii was found as one of the dominant species on all the six sites regardless of elevation or aspect, while Pinus ponderosa was dominant on south slopes only. Abies grandis and A. lasiocarpa were dominant species on north slopes at elevations of 1280 and 1700m, respectively. Moisture, as it related to aspect, was identified as one of the most important environmental gradients for explaining the variation of vegetation types. On north-facing slopes, compared to south-facing slopes, where moisture was not as limiting and canopies could grow denser, probably, elevation or competitive interaction was more important. Species diversity tended to decrease with increasing environmental severity, with south slopes having less diversity than north slopes due to extended water stress and harsher temperature extremes on south slopes. The age structure on north-facing and south-facing slopes was different. Light intensity, moisture and climate were different between these two slopes. Large scale disturbances(e.g., big fire or insects) were major causes in changing age structure. Younger trees showed a closer relationship between size and age than adult trees. DBH values of shade intolerant species in south-facing slope were bigger than those of north-facing slope, which suggested that aspect of stands be the most important factor for age and size.

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Catalytic Spectrophotometry for the Determination of Manganese at Trace Levels by a Novel Indicator Reaction (새로운 지시약 반응에 의해 극미량 수준의 망간 측정을 위한 촉매 반응의 분광 광도 측정법)

  • Gurkan, Ramazan;Caylak, Osman
    • Journal of the Korean Chemical Society
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    • v.54 no.5
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    • pp.556-566
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    • 2010
  • A new kinetic spectrophotometric method is developed for the measurement of Mn(II) in natural water samples. The method is based on the catalytic effect of Mn(II) with the oxidation of Gallocyanin by $KIO_4$ using nitrilotriacetic acid (NTA) as an activation reagent at 620 nm. The optimum conditions obtained are $4.00{\times}1^{-5}\;M$ Gallocyanin, $KIO_4$, $1.00{\times}10^{-4}\;M$ NTA, 0.1 M HAc/NaAc buffer of pH = 3.50, the reaction time of 5 min and the temperature of $30^{\circ}C$. Under the optimum conditions, the proposed method allows the measurement of Mn(II) in a range of $0.1\;-\;4.0\;ng\;mL^{-1}$ and with a detection limit of down to $0.025\;ng\;mL^{-1}$. The recovery efficiency in measuring the standard Mn(II) solution is in a range of 98.5 - 102%, and the RSD is in a range of 0.76 - 1.25%. The newly developed kinetic method has been successfully applied to the measurement of Mn(II) in both some environmental water samples and certified standard reference river water sample, JAC-0031 with satisfying results. Moreover, few cations and anions interfere with the measurement of Mn(II). Compared with the other catalytic-kinetic methods and instrumental methods, the proposed kinetic method shows fairly good selectivity and sensitivity, low cost, cheapness, low detection limit and rapidity. It can easily and successfully be applied to the real water samples with relatively low salt content and complex matrices such as bottled drinking water, cold and hot spring waters, lake water, river water samples.

Analysis of Growth Characteristics and Yield Pattern of 'Cupra' and 'Fiesta' Paprika for Yield Prediction (수량예측을 위한 'Cupra', 'Fiesta' 파프리카의 생육특성 및 수확량 패턴 분석)

  • Joung, Kyong Hee;Jin, Hy Jeong;An, Jae Uk;Yoon, Hae Suk;Oh, Sang Suk;Lim, Chae Shin;Um, Yeong Cheol;Kim, Hee Dae;Hong, Kwang Pyo;Park, Seong Min
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.349-355
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    • 2018
  • This study was aimed at predicting the yield of paprika (Capsicum annuum L.) through analyzing the growth characteristics, yield pattern and greenhouse environment. In the greenhouse of the Gyeongnam area (667 m above sea level), the red paprika 'Cupra' and the yellow paprika 'Fiesta' were grown from July 5, 2016 to July 15, 2017. The planting density was $3.66plants/m^2$ and attracted 2 stems. During the cultivation period, the average external radiation of the glasshouse was $14.36MJ/m^2/day$ and the internal average temperature was controlled as $20.1^{\circ}C$. After 42 weeks of planting, the growth rate of 'Cupra' was 7.3 cm/week and that of 'Fiesta' was 6.9 cm/week. The first fruit setting of 'Cupra' appeared at 1.0th node and 'Fiesta' at 2.7th node. The first harvest of 'Fiesta' was 11 weeks after planting and 'Fiesta' was 14 weeks. Comparing the yield per 10 a until the end of the cultivation in July, 'Fiesta' was 19,307 kg, which was 2.4% higher than that of 'Cupra'. And the fruit weight ratio of over 200 g of 'Cupra' was 27.7% which was 7.7% higher than that of 'Fiesta'. The average required days to harvest after fruit setting of 'Cupra' was 72.6 days and 'Fiesta' was 63.8 days. According to the relationship between the average required days to harvest and the cumulative radiation (during from fruit setting to harvest), the more radiation increases the less required days to harvest increases after February. In terms of yield, 'Cupra' increased in yield as the cumulative radiation increased, while 'Fiesta' showed an irregular pattern. Cumulative radiation from fruit setting to harvest was negatively correlated with required days to harvest after February in both cultivars. But in relation to yield, there were difference between 'Cupra' and 'Fiesta'.

An Approach to Determine the Good Seedling Quality of Grafted Tomatoes (Solanum Lycopersicum) Grown in Cylindrical Paper Pot Through the Relation Analysis between DQI and Short-Term Relative Growth Rate (DQI와 단기 상대생장률 분석을 이용한 원통형 종이포트 토마토 접목묘의 우량묘 기준 설정)

  • Seo, Tae Cheol;An, Se Woong;Jang, Hyun Woo;Nam, Chun Woo;Chun, Hee;Kim, Young chul;Kang, Tae Kyung;Lee, Sang Hee
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.302-311
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    • 2018
  • Using cylindrical paper pot nursery method, three kinds of commercial tomatoes 'Dafnis', 'DOTAERANG DIA' and 'Maescala' were grafted onto a commercial rootstock 'B blocking'. From 10 to 40 days after graft-take, growth traits of seedlings were investigated by 0.5, 1.0 and 2.0S treatments of standard nutrient solution(S) for seedling growth, and top to root ratio(TRR), compactness(CP) and Dickson Quality Index(DQI) were calculated. Two weeks after transplanting of the seedlings under three different night temperature targeting to 10, 15, and $25^{\circ}C$, which were not precisely controlled, the relative growth rate (RGR) was investigated. The quantitative growth traits of grafted seedlings increased with increasing fertilizer concentration, and various range of seedling size could be produced. Compactness and DQI were significantly regressed (Adj $R^2=0.9480$). Short-term RGR after transplanting was higher at 1.0S treatment of standard nutrient solution at the seedling age of 30 days and 40 days after graft-take(DAGT). DQI and RGR were significantly regressed linearly at respective fertigation strength. Specially the diminishing slope of RGR was lower at 1.0S fertigation strength with the increase of DQI than others. The results indicate that DQI could be applied as a quality index of grafted tomato seedlings and the relation analysis between DQI and short-term RGR also could be used to determine the good quality seedlings of grafted tomato grown in cylindrical paper pot.