• Journal of Aquaculture
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• v.6 no.2
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• pp.89-105
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• 1993

In order to investigate the effects of dietary n-3 highly unsaturated fatty acids (n-3HUF A) levels on growth and body composition of the Korean rockfish, six experimental diets with various levels of n-3HUFA, which were adjusted by adding squid liver oil and/or soybean oil at $8\%$ dietary lipid level, were fed to the Korean rockfish (6.2g in mean body weight) for 10 weeks. Daily weight gain, feed efficiency and nutrient retention efficiency were the lowest in the fish fed a diet containing 0% n-3HUFA. These parameters were effectively improved by supplementation with n-3HUFA, and showed linear increase up to $1.2\%$ dietary n-3HUFA level (P<0.01). There was no additional response above this level. A higher concentration of nonpolar lipids in the liver was observed for the fish fed insufficient levels of n-3HUFA in the diets. However the liver glycogen content and hepatosomatic index were slightly deareased. The lipid contents of the whole body and vicera showed significantly higher in the fish fed sufficient levels of n-3HUFA in the diets (P<0.05). The fatty acid compositions of polar lipids in the whole body and liver were affected by dietary fatty acid compositions. The contents of n-3HUFA and 18:1 in the liver increased with increasing the n-3HUFA level in the diets, while the 18:2n-6 and 18: 3n-3 decreased. These results suggest that n-3HUFA plays an essential role for normal growth of the Korean rockfish, and the requirement of n-3HUF A is around $1.2\%$ of the diet.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.5
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• pp.598-605
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• 2005

Effects of chitosan-ascorbate(CA) and calcium lactate(CL) on fermentation and quality of squid sikhae were investigated CA and LA were added at $0.5\%$ (designated CA1 and CL1) and $1.0\%$(CA2 and CL2) concentrations, respectively and fermented for 12 days at $10^{\circ}C$. pH of CA-added sikhae was higher than that of control, while acidity of the former was lower than that of the latter. During 12 days of fermentation, CA-added sikhae showed higher protease activity than control by $2.3\~2.6$ times and CL-added sikhae by $2.8\~3.6$ times. At 12 days of fermentation, CA-added sikhae revealed higher protease activity than control by $1.2\~1.4$ times and CL-added sikhae by $1.5\~1.9$ times. CA-added sikhae also showed higher amino-nitrogen content than control by $1.4\~1.7$ times and CL-added sikhae by $1.9\~3.5$ times. In comparison of CA1 with CA2, CA2 showed all higher pH, protease and amylase activity, and amino-nitrogen content than CA1. In analysis of electrophoresis, molecular weights of major proteins in ~w squid were $116.9\~119.0$, 96.5 and 59.3kDa. However, after fermentation for 12 days, a protein band of 119.0kDa disappeared but a new protein band with below 14 kDa appeared in sikhae, especially CA-added sikhae. In sensory evaluation, the intensity of sour taste was the highest for control and the lowest for CA2. Softness of squid was the highest for control and the lowest for CA2. Overall acceptability was the best for CA2. In conclusion, these results suggest use of $1\%$ CA in sikhae preparation as addition of CA(CA2) increased the protease and amylase activity, nitrogen content of amino form, sensory acceptability as well as shelf-life of sikhae.

• Food Science of Animal Resources
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• v.23 no.3
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• pp.227-235
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• 2003

The results were obtained from pigs which had been fed finishing pig diets containing 5% beef tallow(C) as control and 2% perilla seed oil(Tl), 3% beef tallow and 2% squid viscera oil(T2), 3% beef tallow and 2% CLA(conjugated linoleic acid, T3). All porks were stored at 1$^{\circ}C$ for 28 days. pH value of control group was higher than other treatments. Water holding capacity(WHC) did not show any significant difference among treatments, however, WHC of C and T3 was increased as storage days increased. Protein solubility of T3 was higher than the other treatments, but that of all groups increased up to 14 days of storage and then decreased. The a* value of C was higher than the others, but b* value was low on 28 days of storage. Volatile base nitrogen(VBN) value of T3 showed the highest level, but that of Tl was the lowest. Thiobarbituric acid reactive substances(TBARS) of T2 and T3 were' higher than those of C and Tl. In sensory analysis, meat color and overall acceptability of C were higher than those of the other treatments in raw meat, and meat appearance was higher than level in Tl.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.149-154
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• 1999

A 16-week feeding trial was conducted to determine the amount of fish meal analogue (FMA) that can be replacing white fish meal (WFM) protein in Korean rockfish. Five experimental diets were formulated on isonitrogenous and isocaloric basis of $50\%$ crude protein and 15.6 KJ/g diet. The graded level of replacement of PM by Em on the basis of crude protein were as follows : Diet 1($100\%$ WFM), Diet 2($87.5\%$ WFM : $12.5\%$ FMA), Diet 3 ($75\%$ WFM : $25\%$ FMA), Diet 4($75\%$ WFM : $25\%$ FMA+2 EAAs), Diet 5 ($50\%$ WFM : $50\%$ FMA+2 EAAs). Methionine and Iysine were selected for 2 essential amino acids (EAA) to study the effect of adding EAA to the diets 4 and 5. Weight gain (WG), feed fed efficiency (FE) and protein efficiency ratio (PER) of fish fed diet 3, 4 and 5 were significantly lower (P< 0.05) than those of fish fed the control ($100\%$ WFM),while that of fish fed diet 2 was not significantly different diet(P>0.05) from those of fish fed the control diet. WG, FE and PER of fish fed diet 4 were significantly higher (P<0.05) than those of fish fed diet 3, but lower than those of fish fed the control diet. Therefore, these results indicated that FMA protein could substitute WFM protein up to $12.5\%$ without 2 EAAs supplementation in Korean rockfish. And, dietary supplementation of 2 EAAs could be benetficial when FMA is utilized as a fish meal replacer in juvenile Korean rockfish.

• The Journal of Fisheries Business Administration
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• v.35 no.2
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• pp.91-115
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• 2004

The main purpose of this study is to analyze the cooperative squid fisheries problems surrounding the $E128^{\circ}$, established by the 1965 Korea - Japan Fisheries Agreement which banned Korean large trawlers' fishing east to the $E128^{\circ}$ In fact, the moratorium was put on by Japans request. However, such issue did not occur until the filefish stock in the southern Korean sea, which was a major target fish species of the large trawl fisheries, The filefish stock collapsed completely around 1991 and at the same time most of bottom fish stocks in the East China Sea began to show a symptom of over - exploitation. Thus, the off - shore large trawlers learned to have a little opportunity of finding out alternative fish stocks as well as fishing grounds. Fortunately, at that time squid resource stock and consumption were on the increasing trend. The large trawl fisheries were able to economically exploit squid stock east to the $E128^{\circ}$ through cooperative fishing with squid angling light boats in the East and East - South Sea, even though such cooperative fishing activities violate the existing fishery laws apparently. Some important reasons that the large trawlers have continued the cooperative fishing seem to be because (ⅰ) squid resource stock has been on the increasing state over time, (ⅱ) the trawl fisheries have made a significant contribution to meeting domestic and export demands and stabilizing squid prices, and (ⅲ) they have kept domestic squid market from foreign competition. However, the new Korea - Japan fisheries agreement in 1998 provided a momentum of questioning the effectiveness of the $E128^{\circ}$ by the squid - related fisheries other than the squid angling. Serious conflicts between squid - related fisheries began to emerge and to be much intensified. Squid angling industries in the East opposed to large trawlers's efforts to formalize such illegal cooperative squid fishing activities. Their main argument was that such formalizing would definitely make the East coast squid prices lower and in turn their business performance would be worse off. The results of quantitative analysis suggest that the trawlers' massive landing may have a significant influence on lowering the east coast squid prices. Now, an important issue that the squid - related fisheries and the government are facing is to solve such complex squid fishing problems through a multi - participatory negotiation process, including price stabilization, total allowable catch level and its operation schemes, $E128^{\circ}$ rearrangement, and so on.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.3
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• pp.207-212
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• 1986

Heavy metal contents and residues level of organochlorine pesticides were analyzed for commercial salted and fermented sea-food which bought from pusan area. Heavy metal contents ranged from 0.11 to 0.46 ppm of mercury, trace to 1.1 ppm of lead, 0.01 to 0.34 ppm of cadmium, 0.4 to 5.98 ppm of cupper, 0.16 to 4.5 ppm of zinc, trace to 0.49 ppm of manganese and trace to 1.35 ppm of arsenic. Residue level of organochlorine pesticides in salted and fermented sea-foods ranged from 0.3 ppb to 1.825 ppb of total BHC Organochlorine pesticides of DDE, dieldrine, pp'-DDD and pp'-DDT were detected a small amount from Ge jeot and Myeol-Chi jeot. In conclusion. the heavy metal and levels of organochlorine pesticides residues were safety to eat the salted and fermented sea foods.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.4
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• pp.606-612
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• 2011

The purpose of this study was to investigate certain heavy metals (Hg, Pb, Cd, Cu) in 144 samples of cephalopods, to identify weekly heavy metal intakes and to evaluate potential health risks. The average concentrations in the arm, expressed in mg/kg, were: Hg 0.017 (less than the limit of detection (LOD) to 0.059), Pb 0.024 (less than the LOD to 0.092), Cd 0.030 (less than the LOD to 0.627) and Cu 2.536 (less than the LOD to 5.837). The average concentration in the internal organs, expressed in mg/kg, were: Hg 0.063 (from 0.008 to 0.543), Pb 0.579 (less than the LOD to 2.344), Cd 15.200 (from 0.654 to 75.29) and Cu 201.706 (from 2.412 to 856.4). Heavy metal concentrations were higher in the organs than in the arm. Of the four heavy metals, the ratio of internal organs to arm was highest for cadmium. The weekly intakes of Hg, Pb, Cd and Cu from Octopus minor were 0.2%, 0.08%, 0.20% and 0.00%, respectively from the arm, and 1.0%, 0.96%, 92.28% and 0.05% from the internal organs as compared to PTWI (provisional tolerable weekly intakes) established by the FAO/WHO Expert Committee for Food Safety Evaluation.

• Journal of Aquaculture
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• v.10 no.4
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• pp.417-424
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• 1997

This study was carried out to investigate the effects of experimental formulated diets, commercial diets and natural diet(dried Undaria) on growth and body composition of Haliotis discus hannai. Experimental diets was formulated to provide 34.1% protein and 7.5% lipid, and prepared by different $CaCl_2$ concentration (5, 10 and 15%) and dipping time (10, 20 and 60 second) in solution. The juvenile abalone average weighing 0.3g were distributed in a flow-through aquarium system using a cmpletely randomized desing with 13 diets and 3 replictes per diet (50 abalone/tank), and fed diets for 17 weeks. The abalone fed the time nine experimental formulated diets and two commercial diets showed better growth performance than that of natural diets. No significant differences were found among abalone fed the experimental diets and commercial diets in survival rate, weight gain, soft body weight and soft body composition (P>0.05).

• Korea journal of population studies
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• v.13 no.2
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• pp.19-47
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• 1990

1720년 경상도 단성진 진내면 소재의 390가구를 대상으로 1970년대 초 케임브리지 대학의 역사인구학 연구소에서 개발한 Laslett-Hammel가구 형태 분석법을 적용하여 본 결과 표9에서 제시된바와 같이 전체가구의 48.9%가 핵가족의 형태이며, 42.1 퍼센트가 대가족으로 밝혀지고 있다. 물론 도표3에서 지적되고 있듯이 5세이하의 인구에 대한 자료가 미비한 상태라 호적 자료만으로 당시의 가구 형태에 대한 정확한 분석이 어렵다고 하드라도 이제까지 일반적으로 믿어져 왔던 대가족 형태의 이조 중기의 종가제도나 일반 서민들의 가구 형태 및 유아가구에 대한 보다 구체적인 연구가 호적 자료에 대한 면밀한 분석으로 가능해질것으로 믿어진다. 역촌, 즉 하류계층의 사람들이 주로 많이 주거하였던 단성진 진내면의 전체 조사 대상 가구의 절반이 핵가족의 형태로 보이고 있는것은 당연한 것으로 받아질수 있겠으나 표6에서 나타내고 있듯이 상류계층에 속하는 양소계층의 경우도 평균 가구 규격 16명중에서 약 13명이 율거유아로 실제 가구의 크기는 가족 구성원수 4명을 넘지않는 핵가족의 형태였을 가능성이 짙음을 알수 있다. 한가지 특이할만한 사항은 상류,중류,그리고 하류 계층 다같이 평균 가족 구성원수가 4명을 넘지 않는다는 점이다. 단성진이 독촌들로 구성되어 있는 만큼 앞으로 상류계층이 밀집되어 있었던 경북 월성군의 양동 지역 향방 자료나 호적 초안자료를 단성진의 자료와 비교 분석함으로써 이조 중기의 가구 형태에 대한 보다 신뢰성 있는 결과를 도출해 낼수 이ㅛ는것이다. 단성진의 자료는 이조 중기의 유아 인구와 유아 가구 형태에 대한 연구에는 귀중한 자료로 앞으로 역사인구학의 방법론 개발에 큰 기여를 할것으로 기대된다.의 핑거를 식별, 추적할 수 있었다.는 경도방향의 해구수가 많았으며, 특히 8월은 1년중 경도방향의 분산이 가장 컸고, 어장중심은 5월에는 3888해구, 6월에는 3884해구, 7월에는 4078해구, 8월에는 4154해구, 9월에는 4146해구, 10월에는 4044해구였다. 3. 어획수온과 어획적수온은 5월에는 $14.0~18.5^{\circ}C,$ $15.0~16.0^{\circ}C,$ 6월에는 $13.5~18.5^{\circ}C,$ $14.5~16.0^{\circ}C,$ 7월에는 $14.0~20.0^{\circ}C,$ $14.5^{\circ}C,$ $19.0^{\circ}C,$ 8월에는 $16.0~21.5^{\circ}C,$ $18.0~20.0^{\circ}C,$ 9월에는 $14.5~22.0^{\circ}C,$ $17.0~18.5^{\circ}C,$ 10월 $14.0~18.0^{\circ}C,$ $16.0~17.0^{\circ}C였다.$ 4. 평균CPUE는 5월에는 3.2kg/sheet, 6월에는 4.5kg/sheet, 7월에는 4.3kg/sheet, 8월에는 5.1kg/sheet, 9월에는 6.4kg/sheet, 10월에는 5.8kg/sheet였다. 5. 한국정부의 1990년 북태평양 오징어 어업감시계획과 실제의 어장형성범위를 비교하면 5월에는 어장이 형성된 21개 해구 가운데 12개, 6월에는 24개 가운데 7개, 7월에는 25개 가운데 4개 해구에서

• Korean Journal of Food Science and Technology
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• v.44 no.1
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• pp.1-7
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• 2012

There have been few studies related to ELISA for mackerel. In this study we developed a sandwich ELISA for mackerel in processed foods using rabbit polyclonal antibodies against mackerel parvalbumin, the major allergen of mackerel and heat-stable protein. The parvalbumin was purified by ammonium sulfate precipitation and Sephadex G-50 column chromatography. From the standard ELISA curves, the detection limit of parvalbumin was 3 ng/mL and the detection range of mackerel was 5-5,000 ${mu}g/mL$. We further investigated the cross-reactivity of the antibodies toward mackerel, mackerel pike, salmon, flatfish, armorclad rockfish, cod fish, squid, shrimp, blue crab, and lobster. The antibodies were specific for mackerel only. The mean assay recoveries in cooked cream soup, baby food, sausage, and sauce spiked with 0.01 to 0.3% mackerel were 104, 101, 54, and 0%, respectively. In sample tests of 16 commercial items, the qualitative coincidence ratio of assay result and indication was 75%.