• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.1
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• pp.1-6
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• 2008

The changes in osmolality and the gene expression profiles of prolactin (PRL) and growth hormone (GH) with rapid changes in salinity were compared in the eel (Anguilla japonica), crucian carp (Carassius carassius), and masu salmon (Oncorhynchus masou). Fish stocked in freshwater (FW) were abruptly transferred to experimental tanks containing FW, 50% seawater (50% SW), or 100% SW (SW). Blood samples and pituitary glands were collected 2 and 24 hrs after the exposure. No mortality was observed in SW eel (n=6), whereas all of the crucian carp (n=6) and two masu salmon (n=6) exposed to SW died after land 24 hrs, respectively. The PRL mRNA levels of the eel and masu salmon decreased in 50% SW and SW compared to those of the fish kept in FW after 24 hrs, whereas the PRL levels of crucian carp were higher in 50% SW than in FW. Unlike the PRL mRNA levels, the GH mRNA levels of the eel did not differ significantly among three different salinities, while the GH mRNA levels of crucian carp and masu salmon increased significantly in 50% SW and SW after 24 hrs. The serum osmolalities increased marginally in the eel and masu salmon in 50% SW at 24 hrs (19% and 9%, respectively), whereas those of crucian carp increased abruptly in 50% SW (50% increase). These results suggest that the synthesis of PRL and GH is important in relation to the osmoregulatory system with environmental changes in salinity.

• 한국해양학회지
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• v.30 no.4
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• pp.262-270
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• 1995

A comparative study to understand the changes in physical and sedimentological natures was carried out in the Keum Estuary before and after the gate-close of Keum River weir. After closing of weir-gate maximum tidal current speed decreased about 30∼40% compared with that of the previous gate-opening period. Water masses also represent vertical stratifications both on water salinity and water transparency. The Keum Estuary seems to be changed from the well-mixed type estuary during the gate-opening period to the "partially-mixed type" and/or "salt-wedge type" estuary after the closing of weir-gate. The concentrations of suspended matter range 10∼100 mg/l in surface waters after the gate-close of Keum River Weir, representing about 1/4 to 1/3 decrease than those during the gate-close of Keum River Weir, representing about 1/4 to 1/3 decrease than those during the gate0opening period. Such decrease of suspended mater appears to be due to the decrease in the resuspension of bottom sediments, and also due to the vertical stratification of water masses that prevented the upward diffusion of turbid bottom waters. It is, therefore, expected that the depositional environment of Keum Estuary has been changing into the low energy conditions after the closing of weir gate, resulting in the rapid deposition of fine suspended matters within the Keum Estuary.

• 한국해양학회지
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• v.26 no.2
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• pp.155-168
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• 1991

In order to study the community structure and distribution patterns of primary producers and their relations to abiotic environmental factors, phytoplankton and environmental parameter were determined during the five times from October, 1989 to July. 1990. 159 taxa of phytoplankton have been identified and the most important species group is diatom which contributes to 70.4% of the total number of species. Ratios of phytoplankton group (neritic species, fresh water species, oceanic species) clustered by habitats were 52%, 18%, 12%, respectively, suggesting study area be the typical estuarine environment which is strongly affected by both fresh water discharge and sea water penetration. Species composition could be governed by the variations of salinity. the standing stocks of primary producer in the study area vary with space and time, showing close correlation with water transparency, and exhibit relatively larger than those of other coastal regions in the Yellow Sea. Spectransies diversity also showed large variation with space and time. According to the cluster analysis, the study area could be divided into two regions (inner part and outer part): in fall and winter, outer area affected by the oceanic water, but in spring and summer, inner estuarine area heavily influenced by fresh water.

• The Korean Journal of Zoology
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• v.38 no.4
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• pp.570-579
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• 1995

Ultrastructural and histochemical changes of mucous cells In the freshwater and seawater-adapted guppy (Poecflia reticulatus) gills were observed by the light, scanning-and transmisslon~lectron microscopes. The mucous cells were usually located in the epithelium of primary lameilne projected from the gill arch. The rough endoplasmic retIculum and Golgi complex were hIghly developed In immature mucous cells. The mature mucous cells were nearly filled with the mucous granules. In the freshwater guppy, the histochemical properties of the mucous cells were a mixture of the neutral mucin, sialomucin and sulfomucin. When guppy was adapted to the seawater, the content of acid glycoproteins (slalomucln and sulfomucin) was decreased. In addition, the number of mucous cells in the seawater-adapted group was less than a third of those in the freshwater one. These results suggest that the seawater-adapted guppy would react to the changed osmotic stress of the seawater. And also, the environmental change by the increased salt concentraion might lead to reduce the chance of infections.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.1-5
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• 2009

This study was to investigate the habitat conditions and growth of right eye flounder (Kareius bicoloratus) in the Saemangeum Reseorvoir during 1995$\sim$2007. The planktonic larvae of K. bicoloratus appeared in early March, and then the juveniles started the benthic life in the tide-pools inside the reservoir, being 1.5$\sim$2.5cm in total length in early April and 6.0$\sim$7.2cm in mid-July. This species was not collected in the tide-pools after July, indicating that the juveniles began to move to outer seas for growing. When the tide barrier of Saemangeum was constructed, the juveniles of K. bicoloratus decreased as the tidal pools were covered continuously, then they could not be collected after 2005. Thus, it is concluded that the environmental change has taken important influence on distribution and spawning of K. bicoloratus since the reservoir was constructed.

• Journal of the Korean Society of Food Culture
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• v.11 no.1
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• pp.37-42
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• 1996

We have studied specific properties of magnetic treated water as processing water will make different cookery form the case of piped tap water. The result is as follows; The magnetic treated water both from tap water and pure water became more alkaline than not-magnetic treated water in pH change. As time goes on, magnetic treated or not, pH reduced considerably in piped tap water and increased in pure water. The magnetic treated water showed higher hydration rate than piped tap water in hydration of dried food. According to time, difference of hydration between tap water group and magnetic-treatment water group became significantly. Surface tension of magnetic treated water was slightly lower than that of piped tap water. And it reduced considerably with time. The magnetic treated water showed significantly effective outflow of salt especially in initial phase of soaking in salt-in food.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.82-82
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• 2015

해안 대수층은 해수와 담수가 공존하는 지역으로 상대적으로 밀도가 큰 해수가 대수층의 담수 아래에 쐐기형태로 존재하게 된다. 이러한 쐐기형태의 해수와 담수의 경계면은 압력경도의 평형에 의해 경계면이 유지되며, 해수면 또는 지하수위가 변동할 경우 해수-담수 경계면의 균형이 무너짐과 더불어 압력경도의 평행이 이루어질 때 까지 해수-담수 경계면의 이동이 계속 진행된다. 수위 변화의 주요 원인으로는 지구온난화 및 기후변화로 인한 지속적인 해수면 상승과 도서지역의 인구증가 및 산업화로 인한 무분별한 지하수의 사용 등에 의한 지하수위 저하 등을 꼽을 수 있다. 이와 같은 원인으로 해안 및 도서지역에서는 해안 대수층의 해수침투거리가 증가하여 지하수 이용에 큰 어려움을 겪고 있다. 이에 해안 대수층의 해수침투 범위 및 거리를 추정하기 위한 많은 연구들이 다양한 분야에서 지속해서 이루어지고 있지만, 서로 밀도가 다른 해수와 담수가 공존하는 해안 대수층 내의 수리특성을 명확히 파악하기에는 아직까지 미흡한 점들이 많다. 과거에는 Darcy의 법칙 및 Ghyben-Herzberg 식에 근거한 이론적인 연구들이 주로 이루어졌고, 근래에 현장관측이나 수리모형실험이 국내 외적으로 수행되고 있으나, 모든 영역의 지하수의 특성을 조사하는 것이 사실상 불가능하다. 이에 최근에는 컴퓨터 성능의 비약적인 발전과 더불어 다양한 수치해석방법에 의한 수치모델들이 개발되어 시뮬레이션에 적용되고 있다. 하지만 거의 대부분의 수치모델은 해안 대수층 수리특성을 투수계수에 의존하고 있을 뿐, 대수층 내부의 해수-담수에 의한 밀도류의 유동특성을 전혀 고려하지 못한 채 정수압에 근거한 해수-담수 경계면에 대해 모의하고 있는 정도이다. 따라서 본 연구에서는 해안 대수층 내부의 유동현상을 투수계수에 의존하는 방법에서 탈피하여 대수층 매체의 입경, 공극, 형상 등을 고려할 수 있을 뿐만 아니라, 염분 및 온도차에 의한 밀도류를 해석할 수 있는 강비선형 수치모델을 개발하여 해수침투 현상을 직접 모의한다. 나아가 대부분의 이전 연구들에서 간과하고 있는 해안지역의 대표적 물리력인 파랑과 조석의 영향이 해안 대수층의 해수침투에 미치는 영향, 해안 대수층의 지하수위 및 해수면의 수위차에 의한 해수침투 특성 그리고 이를 제어 할 수 있는 새로운 대응기술을 제안하는 것을 목적으로 한다.

• Journal of Aquaculture
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• v.21 no.4
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• pp.309-316
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• 2008

Effects of the various dietary additives on growth and tolerance of abalone Haliotis discus hannai to the stresses were determined in the 16-week feeding trial. Seventy juvenile (an initial body weight of 4.2 g) abalone per container were randomly distributed into 21, 50 L plastic rectangular containers each. The six kinds of experimental diets were prepared: control (CON) with no additive, by-product of green tea (BPG), extract of figs (EF), extract of green tea (EG), commercially available product of Hearok (PH), and Haematococcus (HC). In addition, dry sea tangle (ST) was prepared to compare the efficiency of the experimental diets. Fishmeal, soybean meal and shrimp head meal were used as the protein source, and dextrin, sea tangle powder and wheat flour, and soybean oil and fish oil were used as the carbohydrate and lipid sources, respectively in the experimental diets. The experimental diets were fed to abalone once a day at a satiation level with a little leftover. The feeding trial lasted for 16 weeks. At the end of the 16-week feeding trial, abalone was exposed to the different types of stresses (air exposure, and sudden changes of rearing temperature and salinity). Survival of abalone fed the sea tangle was highest. However, weight gain of abalone fed the EF, EG and PH diets was significantly (P<0.05) higher than that of abalone fed the BPG diet or dry sea tangle. Shell length of abalone fed the all experimental diets was significantly (P<0.05) higher than that of abalone fed the dry sea tangle. Accumulated mortality of abalone fed the sea tangle was low when exposed to the different types of stresses. Also, relatively low mortality was achieved in abalone fed the HC and EF diets. In considering these results, it can be concluded that the various sources of additives is effective to improve production of abalone, and Haematococcus and extract of figs can be considered as dietary additives to improve resistance of abalone against the different types of stresses.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.3
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• pp.105-114
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• 1974

Identification and change of microflora during the fermentation of anchovy Engraulis japonica, under the halophilic circumstance were investigated. The change of salinity and pH in meat and juice which decide the environment for microorganism and decomposition of nitrogenous compound which functions as a nutrient source were also discussed by measuring the content of total-N, amino-N, nonprotein-N, TMA and VBN, The fresh anchovy was mixed with rock salt (20 percent w/w) and stocked for six months. Through the fermentation lag phase of viable cells extended for 20 days that was obviously larger compared with other circumstances, hereafter increased to reach the maximum value of $5\times10^4$ total count per gram at 35 day stock. The stationary phase proceeded for 25 days. 540 strains were isolated and among them 11 genus of bacteria, 3 genus of yeasts, were identified and other 2 yeast strains of unidentified. At the initial stage of fermentation, Pseudomonas, and Helobacterium prevalently grew, at the middle stage, they disappeared rapidly and Pediococcus and yeasts completely dominated, where they are assumed to get directly involved with fermentation of fish, The PH value tended to decrease in the progress of fermentation and at 100 day stock it showed the minimum value of 5.5 to 5.6 in both meat and juice. The highest salinity of meat decreased to 18 percent, while in juice it decreased to 28 percent since 50 days stock. The content of total-N in meat gradually decreased to 2.8 percent, while in juice it increased to 2.3 percent at 100 day stock, However nonprotein-N was 1.8 percent and amino-N was 1.1 Percent. Since 100 days stock, the increasing rate of amino-M is too low it could be judged to entered the final stage of fermentation, In the first 20 days stock, the increase of VBN and TMA can be explained by the growth of putrefactive bacteria such as pseudomonas on the meat before salts penetrate into the fish meat, while reincrement after 100 days stock, is explained by decomposition of free amino acid due to the reactions of bacteria and enzymes.

• Journal of the Korean Society for Marine Environment & Energy
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• v.18 no.2
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• pp.51-63
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• 2015

In order to investigation long-term variations of water qualities in the Saemangeum Salt-Water Lake formed after the sea-dike construction, the survey has carried out over 40 time from 2002 to 2010. The decreased salinity in surface water immediately after the dike construction has maintained on equal terms for years. After the dike construction, the early concentration of SPM in surface water has decreased but then it showed the tendency to move up and down due to the changes of water level in the lake. The elevated concentration of Chl-a in surface water initially after the dike construction was kept at the same conditions for years. The concentration of DIN in surface water has not changed before and shortly after the dike construction. However, the concentration of $NH_4-N$ in surface water has increased steadily after the dike construction. Consequently the concentration of DIN in the lake water after years has raised compared to pre-dike construction. The reduced concentration of DIP in surface water soon after the dike construction has increased after years as well as $NH_4-N$ due to the accumulation of organic matter to inside lake. Unlike with the unvaried $NO_3-N$, the concentration of DISi in surface water after the dike construction has immediately increased and maintained the enhanced level indicating the supply from other sources except the freshwater. Since the dike construction, the spatial characteristics of water quality was divided river sides and rest of the lake markedly. Stratification of river sides was more strong than the dike sides. In the warm seasons, hypoxia causing the release of nutrients and metals from sediment was observed downward about 1 m from surface of river sides. We strongly suggest to make some urgent measure to prevent low dissolved oxygen condition in the bottom layer of the river sides.