• Journal of Life Science
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• 제27권4호
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• pp.430-434
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• 2017

Essential oils are fragrant oils extracted from the leaves, stems, peels, petals and roots of aromatic plants cultivated by natural means or using organic agricultural techniques. Essential oils have commonly been used as antibacterial and antifungal agents. In the present study, essential oil was extracted from lisianthus (Eustoma grandiflorum [Raf.] Shinn.) and tested for antifungal activities against three eumycetes (Penicillium pinophilum, Chaetomium glogosum and Aspergillus niger). Lisianthus essential oil showed high antifungal activities against three eumycetes, especially against Aspergillus niger, for which the resulting minimum inhibitory concentration (MIC) was 0.005 mg/ml. In addition, the extracted essential oil was shown to have antimicrobial activity against ten intestinal pathogenic bacteria (Escherichia coli, Salmonella typhimurium, Klebsiella pneumonia, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Pseudomonas aeruginosa, Bacillus subtilis, Enterococcus faecalis and Vibrio parahaemolyticus) according to the disc diffusion method and was also shown to exhibit strong antibacterial activity against an additional three pathogenic bacteria (Bacillus subtilis, Listeria monocytogenes and Vibrio parahaemolyticus). These results indicate that lisianthus essential oil could be used as an antibiotic against harmful bacteria that produce intestinal illnesses. From the present study, we suggest that lisianthus extracts can be utilized as potential antifungal and antibacterial agents and for the development of pharmaceutical and cosmetic products.

• Korean Journal of Medicinal Crop Science
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• 제23권3호
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• pp.223-230
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• 2015

To study the effect of crop rotation on the control of ginseng root rot, growth characteristics and root rot ratio of 2-year-old ginseng was investigated after the crops of 18 species were cultured for one year in soil contaminated by the pathogen of root rot. Fusarium solani and Cylindrocarpon destructans were detected by 53.2% and 37.7%, respectively, from infected root of 4-year-old ginseng cultivated in soil occurring the injury by continuous cropping. Content of $NO_3$, Na, and $P_2O_5$ were distinctly changed, while content of pH, Ca, and Mg were slightly changed when whole plant of crops cultured for one year were buried in the ground. All of EC, $NO_3$, $P_2O_5$, and K were distinctly increased in soil cultured sudangrass, peanut, soybean, sunnhemp, and pepper. All of EC, $NO_3$, $P_2O_5$, and K among inorganic component showed negative effect on the growth of ginseng when they were excessively applied on soil. The growth of ginseng was promoted in soil cultivated perilla, sweet potato, sudangrass, and welsh onion, while suppressed in Hwanggi (Astragalus mongholicus), Deodeok (Codonopsis lanceolata) Doraji (Platycodon grandiflorum), Gamcho (Glycyrrhiza uralensis), Soybean. All of chicory, lettuce, radish, sunnhemp, and welsh onion had effective on the inhibition of ginseng root rot, while legume such as soybean, Hwanggi, Gamcho, peanut promoted the incidence of root rot. Though there were no significant correlation, $NO_3$ showed positive correlation, and Na showed negative correlation with the incidence of root rot.

• Culinary science and hospitality research
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• 제19권3호
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• pp.73-86
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• 2013

This study produced hot-air-dried perilla leaf to add Korea's popular perilla leaf to fresh noodles to examine their value as food and get the best fresh noodle recipe by adding 0%, 3%. 6%, 9%, and 12% of perilla leaf to flour. The moisture content of the dough was highest at 34.41% for the control group with 0% perilla leaf, whereas the pH was lowest at 5.59 for the dough with 12% perilla leaf. As a result of WRC analysis of the dough, moisture absorption increased with greater perilla leaf content. The moisture content of fresh noodles was lowest for the control group with 0% perilla leaf, whereas the pH was highest for the control group. The L, a, and b values of the dough were highest for the group with 0% perilla leaf powder. Hardness and cohesiveness were lowest for the 0% group and springiness tended to decrease with increased amounts of hot air-dried perilla leaf. The number of microorganisms decreased significantly with greater perilla leaf content on the third and fourth days. As a result of the sensory test, the 6% dough showed the highest scores for all items including appearance, flavor, color, taste, and texture. Overall acceptability was also highest at 7.20 for the 6% dough and lowest at 3.27 for the 12% dough. Based on the above results, when producing fresh noodles by adding hot-air-dried perilla leaf powder, inhibition of microorganisms improved with greater perilla leaf content for longer storage, but the sensory properties were best when 6% perilla leaf content was added.

• Korean Journal of Agricultural Science
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• 제28권2호
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• pp.99-107
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• 2001

It is difficult to install a ventilation window on the roof of single span greenhouses of arch shape. Investigation on the roof ventilation structures for those greenhouses was conducted. In small greenhouses with spans of 5 to 8 m, circular or chimney type ridge vents made of plastic were employed. In large greenhouses with spans of 12 to 18 m, even span roll-up ridge vents made of steel pipe were employed. The effect of roof ventilation was evaluated by comparative experiments between greenhouse installing ridge vents and having controlled side vents only. Roof ventilation contributed greatly to restraint of temperature rise and maintenance of uniform temperature distribution in greenhouses. And ventilation efficiency was analyzed by experiments on the opening and closing operation of the ridge and side vent. There were no temperature differences according to opening and closing sequence of ventilation window. But for greenhouse temperature control by ventilation, it is desirable to open side vents after ridge vents and to close ridge vents after side vents.

• Journal of the Society of Cosmetic Scientists of Korea
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• 제42권3호
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• pp.227-233
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• 2016

This study was designed to analyze the chemical compositions of 'Setoka' branch essential oils (SEBO) and to test their biological activities. 'Setoka' is a Citrus species widely cultivated in Jeju Island. At the present, 'Setoka' branches produced by thinning process were mostly discarded as a waste. Therefore, utilization of this branch waste has received much attention. 'Setoka' branch essential oils (SBEO) were prepared by treatment of its ethanol extracts with jojoba oil. SBEO were chemically analyzed using gas chromatograph-mass spectrometry (GC-MS), and following components were identified; ethyl linoleate (64.14%), ethyl palmitate (16.50%), neophytadiene (11.06%) and beta-citronellol (5.09%). The anti-inflammatory activity in the SBEO was examined using RAW 264.7 murine macrophage cells stimulated with LPS. As a result, the SBEO inhibited nitric oxide (NO) productions with a dose-dependent manner. In addition, SBEO showed good anti-microbial activities against drug-susceptible and -resistant skin pathogens such as Staphylococcus aureus and Propionibacterium acnes, which are acne-causing bacteria. Based on these results, we suggest that SBEO has the possibility for use as an anti-inflammatory and anti-microbial agent in cosmetic applications.

• Korean journal of applied entomology
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• 제26권2호
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• pp.89-97
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• 1987

Antagonistic microorganisms from rhizosphere soil were isolated, identified, and applied successfully as the biocontrol agents of damping-off caused by Rhizoctonia spp. Rhizosphere antagonists isolated from rhizosphere soil were identified as Trichoderma viride, T. harzianum, T. hamatum, T. polysporum, Gliocladium sp., Pseudomonas fluorescence, P. stutzeri, P. cepacia, Enterobacter sp., Serratia sp. and Erwinia herbicola. Of these, the most promising ones in vitro were T. virdie, T. harzianum, Gliocladium sp., Serratia sp., P. stutzeri, and P. cepacia. These above six antagonists were efficient in reducing disease incidence to $40{\sim}70%$ when the reselected rhizosphere antagonists preparations were applied to the soil at $10^6$ propagules per gram. Among six antagonists, T. viride was the most promising biocontrol agents against R. solani isolates in soil. The suppressive effect was more evident in steam-sterilized soil than in non-sterilized field soil.

• Asian Journal of Turfgrass Science
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• 제15권4호
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• pp.169-179
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• 2001

This study was conducted to determine the effect of root zone cooling on growth and quality of turfgrasses including Kentucky bluegrass (Poa pratensis L.‘Nuglade’), perennial ryegrass (Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb.‘Pixie’), and Japanese lawngrass (Zoysia japonica Steud.) in simulated athletic field during summer season in Korea. Mineral contents in clippings of turfgrasses grown at different soil mixtures and temperatures were also analyzed. Root zone cooling (approximately 4~6$^{\circ}C$ lower than that of untreated-control) resulted in good uniformity, little disease incidence and higher level of chlorophyll contents in cool-season turfgrasses. The effectiveness of root zone cooling in protecting disease incidence from high temperature stress was the most manifest in perennial ryegrass compared to others. Fresh clipping weight in treatment of root zone cooling was increased approximately 2 times in Kentucky bluegrass and perennial ryegrass, and 2.5 times in tall fescue compared to those of control. There was higher growth rate in a soil mixture composed of 80% peat moss ＋10% sand ＋10% soil (v/v/v) than in that of 80% pea moss ＋20% sand (v/v), Mineral contents of N, P, K, Ca, and Mg in clippings of three species of cool-season turfgrasses were significantly increased in treat-ment of root zone cooling but this was not found in Japanese lawngrass. Results showed that root zone cooling has a benefit in keeping good quality and growth of cool-season turfgrasses in sports field under supraoptimal ambient temperature during summer season.

• The Korean Journal of Mycology
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• 제46권1호
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• pp.58-68
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• 2018

Sunn hemp (Crotalaria juncea) is used as a nitrogen-fixing green manure in Korea to improve soil quality, reduce soil erosion, and suppress weeds and nematodes. In 2014, wilting sunn hemp plants were observed in green manure-cultivated fields in Wanju, Korea. Leaves of the infected plants began yellowing, starting with the lower leaves, eventually leading to their death. Moreover, a number of dark perithecia were observed on the wilting stems. Six isolates were obtained from these perithecia by single spore isolation. Based on their morphological characteristics, the isolates were identified as Fusarium udum (teleomorph: Gibberella indica). Macroconidia were slightly curved with almost hooked apical cell, and microconidia were formed on false heads by monophialides. Chlamydospores were produced abundantly in the hyphae, either singly or in clusters. To confirm the identification, multilocus sequence analysis was conducted using translation elongation factor 1 alpha (TEF), calmodulin (CAL), and histone 3 (HIS3). The sequences of TEF, CAL, and HIS3 showed 94.4~96.2%, 99.7%, and 99.6~99.8% similarity to the reference sequences of F. udum in NCBI GenBank, respectively. Pathogenicity was tested on sunn hemp and two soybean cultivars using the inoculation method of soil drenching with spore suspension. The wilting symptoms were observed only in sunn hemp and one cultivar of soybean (cv. Teagwang) after 14~21 days of inoculation. This is the first report of wilt disease in sunn hemp caused by Fusarium udum in Korea.

• Korean Journal of Medicinal Crop Science
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• 제2권1호
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• pp.20-25
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• 1994

This study was carried out to investigate the effect of temperature and daylength on the bolting of Angelica gigas Nakai for decreasing the rate of bolting.Seedlings were transplanted on early December to the PVC pot(1 /1000) in the greenhouse con­trolled the temperature of 15, 20 and $25^{\circ}C$, and the daylength of 8, 12, and 16hrs. Growth of aerial parts, bolting rate and root characteristics of A. gigas were investigated. Seedlings of A. gigas untreated with low temperature were nomally emerged, bolted and flowered in maturing stage. In this results, it was assumed that A. gigas was low sensitive to low temperature. The early growth stage was good at $20^{\circ}C\;and\;25^{\circ}C$, but the late growth was effective at $15^{\circ}C\;and\;20^{\circ}C$. The bolting rate was the highest at $20^{\circ}C$ while the root weight was the lowest. Although the bolting rate was high at $15^{\circ}C$, the yield was increased by the delay of bolting and flowering stage. The bolting rate was increased in the longer daylength and showed more sensitive to the daylength condition than to the temperature. Thus, A. gigas cultivated in gully field with cool temperature or under the shading light could be reduced could the bolting rate.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제42권2호
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• pp.166-172
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• 1997

This experiment was carried out to clarify the effect of the deep water irrigation on dry-seeded rice cultivation at the three different water managements-deep continuous flooding(DCF), water saving irrigation(WSI), ordinary irrigation(OI). The highest tillering numbers per $m^2$ of rice were 551, 466 and 455 in OI, WSI and DCF, respectively. The tillering number of rice plants were significantly reduced in DCF. Heading date was delayed and the total chlorophyll content in leaf after heading was higher in DCF than those in other irrigation methods. For the characteristics associated with lodging, the culm length in DCF was slightly elongated and the diameter of culm in DCF was thicker than that in WSI and OI. The breaking weight and bending moment in DCF also were higher than those in others. As the result, although the culm length in DCF was long, the lodging index was comparatively low. The panicle length in DCF was longer than in OI and WSI. The spikelet number per $m^2$ and 1,000-grain weight were the most in WSI, while panicle number, ripened grain ratio and grain weight were not significantly different. Longer panicle length and more spikelet number resulted in higher yielding capacity in DCF.