• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.337-342
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• 2009

Present studies were conducted to evaluate the effects of medium strength(MS and Hyponex), carbon sources and their concentrations, agar concentrations, and inoculation amounts on prothallus propagation of Pterdium aquilinum var. latiusculum(Desv.) Underw. ex Hell in vitro. The optimum MS medium strength for prothallus propagation was 2MS concentration. Phosphate source was most effective for prothallus growth of P. aquilinum var. latisculum. The addition of 1% sucrose or glucose to MS medium promoted prothallus multiplication. Growth of prothallus was not affected by agar concentration. Propagation of homogenized prothallus was vigorous even in liquid medium. Chopped gametophytes(100 and 200 mg) were inoculated on 250 ml ${\Delta}$flask with 100 mL of 2MS concentration medium and suspension culture was done at 100 rpm for 22 days. After 20 days, prothallus multiplication slowed down, so 100 mg of chopped prothalli is recommended for initial inoculation, since initial amount of inoculum did not affect subsequent prothallus multiplication. Consequently after 20 days of suspension culture, prothallus should be subcultured or transplanted outside of growing vessels.

• Journal of Environmental Impact Assessment
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• v.24 no.1
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• pp.51-65
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• 2015

The flora distributed around Mt. Hwangseok were summarized as 495 taxa including 97 families, 295 genera, 394 species, 4 subspecies, 80 varieties and 17 forms. In the component ratio of taxa, pteridophyta was 4.2%, 1.2% of gymnospermae, 78.8% of dicotyledonae and 15.8% of monocotyledonae. The rare plants were 9 taxa including Aconitum chiisanense, Eranthis stellata, Aristolochia contorta, Viola albida and so forth. The Korean endemic plants were 13 taxa including Populus tomentiglandulos, Pseudostellaria coreana, Aconitum pseudolaeve, Thalictrum actaefolium var. brevistylum and so forth. The specific plants by floristic region were 16 taxa including Cimicifuga heracleifolia, Sanguisorba longifolia, Symplocarpus renifolius, Betula davurica and so forth. The plants adaptable to climate change were 11 taxa including Aconitum pseudolaeve, Ajuga spectabilis, Carex okamotoi, Rhynchosia volubilis and so forth. The naturalized plants were 20 taxa including Fallopia convolvulus, Rumex crispus, Phytolacca amrericana, Robinia pseudoacacia and so forth. The invasive alien plant was Aster pilosus. The emergence of local ecologically important species, set the correct range for the core and buffer zone, transition zone, coded will be necessary, the distribution of the specialty plant areas and rare plants, an area management plan should be organized by nature. In addition, a variety of assessment techniques and accurate data to establish the validity of the core area is also set to acquire.

• Journal of fish pathology
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• v.19 no.3
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• pp.267-276
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• 2006

Seventy-five streptococci were isolated from diseased olive flounder, Paralichthys olivaceus in Jeju. Their drug susceptibility and transferable multiple drug resistance were characterized. All isolates were resistant to flumequine (AR) and oxolinic acid (OA) and 26 isolates (34.7%) showed 4～6 multiple resistance of ampicillin (ABPC), AR, doxycycline (DOXY), erythromycin (EM), norfloxacin(NOR), OA and oxytetracycline (OTC) in various combinations. pST9 of a transferable R plasmid was detected from a multiple drug resistance strain, Streptococcus sp., ST9 originated from diseased flounder in Jeju, previously. We performed DNA hybridization to know the distribution of plasmid with the same DNA structure as pST9 in streptococci. Thirteen out of 60 isolates analyzed were positive in colony DNA hybridization and the part of bacteria isolated from raw meal was also hybridized with pST9. It suggested that raw meal is one of the origin of the resistance plasmid and R plasmid with DNA structure differing from pST9 is also involving in multiple drug resistance of the streptococci. In conjugation experiment, we found transferable R plasmid carrying OTC, DOXY and/or EM resistance determinant in the 13 resistance strains. all of the streptococci carrying the transferable R plasmid were similar in RAPD patterns. However, pST -type R plasmid was rare in S. iniae most frequently appearing in flounder farm.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.6
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• pp.2629-2635
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• 2011

This study carried out questionnaire survey and experiment on oral hygiene effects according to the actual condition of using oral care products and the amount of toothpaste used targeting 40 freshmen and sophomores in dental hygienics in some universities from November 8, 2010 to November 26. The collected data was analyzed by using SPSS WIN 15.0 program. The following conclusions were obtained. 1. The result of actual condition of toothbrushing is as follows. As the answer of a question about whether or not toothbrushing after every meal at ordinary times, the largest was 47.5% of being average. The occasion of toothbrushing before going to bed at ordinary times was the largest with 47.5%. The daily toothbrushing frequency was 3 times mostly and the general toothbrushing method at ordinary times was a rolling method. The frequency of exchanging toothbrushes for the past 1 year was 3 times, which is the largest with 45.0%. 2. As a result of the actual condition of using oral care products, the majority of students don't use electric toothbrush, interdental brush, or mouth-rinse except dental floss. Accordingly, there need to be education and promotion with diverse methods so that a patient oneself can prevent oral disease by selecting and using oral care products that are proper for each individual. Also, it was indicated that there is no big difference in oral hygiene effects depending on the amount of toothpaste used. Thus, to avoid wasting toothpaste, we need to educate and promote to use the amount of toothpaste under 0.3g.

• Journal of the Korean Institute of Landscape Architecture
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• v.32 no.6 s.107
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• pp.103-108
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• 2005

It was aimed to promote as a material for interior landscape by validating Rumohra aristata, in an indoor environment, especially irrigation interval, medium composition and drainage at indoor. 1. The result of physico-chemical analysis of medium composition showed that porosity, pH and Ex-Ca, Ex-Mg and Ex-K were high with peatmoss: vermiculite: perlite(1:1:1) and water contents, organic matter content, total nitrogen and cation exchange capacity were high with sand: leaf mold(1:1). 2. Growth and indoor adaptability of Rumohra aristata were better with irrigation at 2 interval per week than irrigation at 7 interval per week regardless of drainage. 3. In the case of medium composition, of growth and indoor adaptability were higher with sud: leaf mold (1:1) than peatmoss: vermiculite: perlite(1:1:1). 4. Fronds fresh weight and dry weight decreased when irrigation interval increased and were higher with sand: leaf mold(1:1) than peatmoss: vermiculite: perlite(1:1:1) treatment.

• Korean Journal of Plant Taxonomy
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• v.37 no.4
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• pp.545-563
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• 2007

The investigated vascular plants of Dok-do island were confirmed that consisted of 46 species, 1 subspecies, 1 varieties in total of 48 taxa growing spontaneously. 1 taxa of Pteridophyta, 36 taxa of Dicotyledon and 11 taxa of Monocotyledon identified. In 48 identified taxa in this study, total 13 taxa included Orobanche coerulescens Stephan, Fallopia sachalinensis (F. Schmidt) Rouse Decr., Lonicera insularis Nakai identified according to the Korean plant taxa for environmental assessment. Planting species were about 10 taxa included Pinus thunbergii Parl., Hibiscus syriacus L. and etc. Four species confirmed in this study such as between Chenopodium virgatum Thunb. and Chenopodium album L., Echinochloa crusgalli (L.) P.Beauv. and Echinochloa crusgalli var. oryzicola (Vasinger) Ohwi. were doubt on identification in taxonomic entity due to morphological characteristics. The dispersion type of Do-kdo plants were anemochore 31 taxa, zoodchore 8 taxa, hydrochore 2 taxa and the others by artificial means.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.1
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• pp.91-101
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• 2012

This study's purpose is to research the antioxidant power of Calaguala (Polypodium leucotomos, PL) as an oral supplement: Calaguala, a native of Central America, is a fern which belongs to the family polypodiaceae protecting the skin from the ultraviolet and active oxygen free radicals. The total 14 volunteers, each female 7 & male 7, were divided into 2 groups, then exposed to UV for 30 min after taking Calaguala and placebo. The total antioxidant capacity (TAC) was analyzed by taking the blood samples from anticubital vein 3 hours after UV exposure. First, Calaguala siginifcantly decreased the concentration of active oxygen free radicals 4 weeks later. The concentration of active oxygen free radical in PL group declined more than placebo group, when measured 30 min and 3 hours after UV exposure. Second, Calaguala did not significantly increase TAC 4 weeks later, but significantly decreased TAC 3 hours after exposure, when compared with placebo group. Now days, the photo allergy and photo aging caused by air pollution and ozone destruction have drastically increased, and thus the amount of UV radiation reaching the earth's surface increased. The increase of active free oxygen radicals resulted in accelerating the diseases and aging. The first superficial protecting protection by reflection & dispersion and the second body internal protection by taking oral photo antioxidant such as Calaguala should be continuously researched.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.201-211
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• 2007

The vascular plants at the nature every second year in Wolchulsan National Park was listed 325 taxa: 86 families, 205 genera, 283 species, 1 subspecies, 36 varieties and 5 forms. Based on the list of the rare plants by the Forest Research Institute, 3 taxa were recorded in the studied areas; Lilium callosum (Preservation priority order; No. 191), Viola albida (No. 202), Wikstroemia trichotoma (No. 120) and based on the list of Korean endemic plants, 8 taxa were recorded; Carex okamotoi, Lilium amabile, Carpinus coreana, Clematis trichotoma, Stewartia koreana, Ajuga spectabilis, Weigela subsessilis, Adenophora triphylla var. hirsute. Specific plants by floral region were total 44 taxa; Prunus davidiana, Wistaria floribunda in class IV, 9 taxa (Neolitsea aciculata, Vaccinium bracteatum, Utricularia racemosa, etc.) in class III, 5 taxa (Bupleurum longiradiatum, Ostericum melanotilingia, Cirsium schantarense) in class II, 28 taxa (Polygonatum falcatum, Eurya japonica, Ajuga spectabilis, etc.) in class I. The naturalized plants in this site were 4 families, 6 genera, 9 taxa and naturalization rate was 2.8% of all 325 taxa vascular plants.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.113-124
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• 2007

The vascular plants of the studied area in Keum River lower stream were listed 238 taxa: 74 families, 179 genera, 211 species, 24 varieties and 3 forms. In this, Woody plant were 60 taxa (25.2%), Herb plants were 178 taxa (74.8%) of all 238 taxa vascular plants. Pteridophyta were 4 families, 4 genera, 4 taxa, Gymnospermae were 2 families, 2 genera, 3 taxa and Angiospermae were 68 families, 173 genera, 231 taxa. Based on the list of Korean endemic plant, 2 taxa were recorded; Populus tomentiglandulosa, Forsythia koreana. Specific plant species by floral region were total 7 taxa; Salix siuzevii in class III, 6 taxa (Ainus hirsute, Quercus variabilis, Mallotus japonicus, Plantago camtschatica, Actinostemma lobatum, Sagittaria aginashi) in class I. The naturalized plants in this site were 14 families, 35 genera, 40 species, 2 varieties, 42 taxa and naturalization rate was 17.6% of all 238 taxa vascular plants. Wild plants disturbing ecosystem like Ambrosia artemisiifolia var. etatior have been increasing. So, it needs continuing control and conservation measures on the plant ecosystem.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.54-54
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• 2018

숨은물뱅듸 산림유전자원 보호구역은 해발 950 m 이상 지역의 습지로서 오름으로 둘러싸인 넓은 웅덩이 형태로 환경부 멸종위기 2급 야생식물인 자주땅귀개를 비롯한 다양한 습지 식물과 이를 둘러싸고 있는 산림지역을 포함하여 산림청에서는 산림유전자원 보호구역으로 지정 관리되고 있다. 본 조사는 숨은물뱅듸 산림유전자원 보호구역을 대상으로 식물상을 조사하여 식물종 다양성을 보존하기 위한 기초자료를 만들기 위해 실시하였다. 본 연구는 2018년 7월 24일부터 8월 28일까지 총 4회에 걸쳐 현장조사를 통하여 표본을 채집하고 기록하여 정리하였다. 그 결과, 숨은물뱅듸에 자생중인 식물은 양치식물 8과 11속 17종 17분류군, 나자식물 2과 2속 2종 2분류군, 피자식물 56과 121속 167종 5변종 1품종 173분류군 총66과 134속 186종 5변종 1품종의 총 192분류군이 조사되었다. 이 중 환경부 멸종위기야생식물은 자주땅귀개 1종이 확인되었고, 제주특산식물 6분류군, 한국특산식물 2분류군이 확인되었다. 식물구계학적특정식물은 총37분류군이며 V등급 5분류군, IV등급 5분류군, III등급 12분류군, II등급 5분류군, I 등급 10분류군이 확인되었다. 한국의 적색목록 식물은 위기(EN) 1분류군, 취약(VU) 1분류군, 준위협(NT) 1분류군, 관심대상(LC) 6분류군, 미평가(NE) 3분류군으로 나타났다. 조사된 식물들 대상으로 생활형을 분석해보면, 휴면형은 Ch 47분류군으로 가장 많이 나타났고, G(30분류군), MM(24분류군), HH(23분류군) 순으로 나타났다. 번식형은 R5가 101분류군, 산포기관형은 D4가 84분류군, 생육형은 e가 89분류군으로 가장 많이 나타났다. 반면, 외래식물 1분류군이 출현한 것으로 보아 숨은물뱅되는 아직까지 보전이 잘 되어 있고, 식물종다양성이 우수하며 식물학적으로 가치가 매우 높은 것으로 판단되었다.