• Title/Summary/Keyword: 양자수율

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Increased biomass and enhanced tolerance to salt stress in Chinese cabbage overexpressing Arabidopsis H+-PPase (AVP1) (애기장대 H+-PPase(AVP1) 과발현 배추에서 바이오매스 증가와 내염성 향상)

  • Park, Mehea;Won, Hee-Yeun;Kim, Chang Kil;Han, Jeung-Sul
    • Journal of Plant Biotechnology
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    • v.39 no.4
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    • pp.253-260
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    • 2012
  • On the basis of the reported agriculturally valuable phenotypes resulted from ectopic overexpression of Arabidopsis vacuolar $H^+$-PPase (AVP1), we generated the Chinese cabbage lines expressing AVP1 which then subjected to salt stress to determine the AVP1 expression if it consistently confers the capability for increasing biomass and enhancing tolerance to salinity in other species. Collectively, here we demonstrate that the transgenic young plants show more vigorous growth and higher tolerance to salt stress than wild-type ones. Increased biomass phenotype by AVP1 expression was supported by comparing fresh and dry weights of transgenic and wild type plants grown under normal condition, while higher salt tolerance trait was confirmed by tracing the kinetics of photosystem II quantum yield and DAB-staining under gradually intensified salt stress induced by MS salt or NaCl, followed by normal condition.

Aqueous Photolysis of the Organophosphorus Insecticide Flupyrazofos (유기인계 살충제 Flupyrazofos의 수중 광분해)

  • Kim, Kyun;Kim, Yong-Hwa;Lee, Jae-Koo;Jeong, Yun-Ju
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.130-135
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    • 2000
  • Photodegradation rate of flupyrazofos in aqueous solution were measured under various test conditions mainly following the guidelines of U.S. EPA and OECD. It was observed that the flupyrazofos was degraded by simple hydrolysis but not degraded by light in pure water. Using acetone as a known photosensitizer, the minimal concentration of acetone needed to photo-degrade the flupyrazofos in % and molar terms were 0.047% and 0.006, respectively. When treated with acetone, it was also found that the ringlet oxygen is a very effective photo oxidant in the degradation of flupyrazofos, but the effect of hydroxyl radical was not observed at the treatment level of hydroxyl radical, isopropylbenzene. In an actinometer experiment, quantum yield of flupyrazofos (0.4 ppm with 2% acetone) was $17.66{\times}10^{-5}$ and degradation rate and half-life were 0.038/hr and 18.2 hours, respectively.

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A Study of the Photoelectrochemical Effects of Squaraine Aggregate in Monolayer (스쿠알렌 집합체의 광전기화학적 효과에 관한 연구)

  • Young Soon Kim;Kock-Yee Law;David G. Whitten
    • Journal of the Korean Chemical Society
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    • v.37 no.7
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    • pp.642-647
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    • 1993
  • Monolayers of pure surfactant squaraine, DSSQ(4-distearyl amino phenyl-4'-dimethylaminophenylsquaraine), were deposited on $SnO_2 $ electrodes by the Langmuir-Blodgett film technique. The DSSQ film exhibits ${\lambda}_{max}$ at ∼530 nm. The absorption is significantly red-shifted from the solution of DSSQ (633 nm in chloroform), suggesting that the squaraine chromophores form aggregates in the LB film. The photogeneration of the squaraine aggregates is studied by measuring the photocurrents in photoelectrochemical cells consisting of the squaraine of the aggregates is found to parallel its absorption spectrum and quantum efficiency as high as 0.3% has been observed. While the photocurrent was attenuated exponentially when stearic acid layers (up to 8 layers) are inserted between the squaraine layer and the electrode, it is nearly extinguished when the squaraine layer is over-coated with 2 layers of stearic acid. A model for the observation is proposed and the roles of the electrolytes and oxygen on the photogeneration process will be discussed.

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The Effects of Air Temperature and Age on Soundness of Abies koreana Seeding (대기 온도와 연령이 구상나무 유묘의 건전도에 미치는 영향)

  • Seung-Beom Chae;Han-Na Seo;Hyo-In Lim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.50-50
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    • 2020
  • 기후변화의 영향으로 자생지에서 고사하고 있는 구상나무 유묘의 대기온도와 연령에 따라 건전도를 파악하고자 지리산 구상나무 실생묘를 대상으로 엽록소형광을 측정하였다. 2020년 7, 8, 9월에 걸쳐 월별 1회씩 연생별(1, 2, 3, 5, 6년생) 20개체를 대상으로 13시, 19시에 개체당 3회 반복 측정하였다. 측정 당시 평균대기온도는 24.6, 26.9, 20.6℃이었으며 잎을 20분간 광을 차단 후 Fv/Fm값을 측정하였다. Fo는 식물이 암적응 된 상태에서 포화광을 조사하기 직전의 형광 초기값이며, Fm은 식물이 암적응 된 상태에서 포화광을 통해 유도된 최대 형광수치를 의미한다. Fv는 Fm-Fo이며, 광화학 반응의 최대 양자수율로 식물의 잎이 광합성을 수행할 수 있는 잠재력을 의미하고 식물의 광합성 활성과 건전도에 대한 지표로 사용된다. 일반적으로 건강한 잎의 Fv/Fm값이 0.8을 기준으로 낮은 값은 광계II의 반응 중심이 손상을 잎은 경우이거나 스트레스 환경에 있는 경우이다. 13시 측정 결과 8월 측정값 중 1년생과 3년생이 0.8 이하로 측정되었고 그 외에는 전부 0.8 보다 높게 나타났다. 19시 측정 결과 8월에 1, 2, 3년생이 0.8 이하로 측정되었으며, 9월에는 1년생의 경우를 제외하고 모두 0.8 보다 높게 나타났다. 1, 2, 3년생은 8월의 높은 온도에 영향을 받아 고산지대에 자생하는 구상나무의 특성상 고온에 대한 스트레스를 받은 것으로 판단되며, 연령이 비교적 높은 5, 6년생은 온도변화에 비교적 안정적인 것으로 나타났다. 따라서 엽록소형광 측정의 결과를 근거로 3년생 이하의 개체는 온도변화에 민감하게 반응하는 반면, 5년생 이상의 개체는 비교적 안정적으로 건전도가 유지된 것으로 판단되었다.

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Effect of Growth Temperature and MA Storage on Quality and Storability of Red Romaine Baby Leaves (생육온도와 MA저장이 적로메인 상추 어린잎의 품질과 저장성에 미치는 영향)

  • Choi, Dam Hee;Lee, Joo Hwan;Choi, In-Lee;Kang, Ho-Min
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.27 no.3
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    • pp.187-192
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    • 2021
  • This study was conducted to compare the quality of baby leaves grown under several temperature conditions and the storage properties of MA storage for romaine lettuce. It was grown for 5 weeks under an artificial light source (200 µmol·m-2·s-1) in a chamber at 21℃, 28℃, and 35℃. The growth and quality of red romaine lettuce that grown in different temperatures were investigated at the end of cultivation, and the oxygen, carbon dioxide, and ethylene concentrations in the 20,000 cc OTR film and perforated film packed with lettuces were measured for 36 and 12 days, respectively. The red romaine lettuce baby leaf was examined for color, chlorophyll, and visual quality at the end of storage. The maximum quantum yield of baby leaf grown in different temperatures at 7days before the harvest was higher at 21℃ and 28℃ growth temperature treatments. On harvest day, the leaf length measured was longest at 28℃, and the leaf width was wider at 21℃ and 28℃, and the number of leaves was similar to 5-6 at all cultivation temperatures. Leaf weight, root weight, and dry weight were found to be higher at 21℃, and tended to decrease as the cultivation temperature increased. The concentration of ethylene in the film of the MA storage treatments was maintained at 1~2 µL·L-1 until the end of storage in all treatments regardless of the cultivation temperature. Oxygen concentration in the MA treatment used 20,000 OTR film was maintained at around 19.5%, and carbon dioxide concentration around 1% that was satisfied the CA conditions. Both Hunter a* and b* values were generally higher in the MA storage treatment at the end of storage day. The chlorophyll content was decreased as the cultivation temperature increased, and was lower in the MA storage treatment than in the perforated film treatment. Visual quality was 3 points or higher in the MA storage treatment at 21℃ growth treatment, and it was maintained marketability. As the above results, the growth of baby leaves of romaine lettuce was the best at 21℃ treatment, and the lower the cultivation temperature, the longer the shelf life. And it was possible to extend the shelf life by 3 times by showing excellent visual quality at the MA storage treatment that satisfies the carbon dioxide concentration of CA condition until the end of storage day.

Effects of Shading Treatment on Photosynthetic Activity of Acanthopanax senticosus (차광처리가 가시오갈피의 광합성 활성에 미치는 영향)

  • Kim, Pan-Gi;Lee, Kab-Yeon;Hur, Seong-Doo;Kim, Sun-Hee;Lee, Eun-Ju
    • The Korean Journal of Ecology
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    • v.26 no.6
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    • pp.321-326
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    • 2003
  • This study was conducted to investigate and effects of shading treatment on photosynthetic activity of Acanthopanax senticosus. We investigated plant growth, light response curve and A-Ci curve to photosynthesis of A. senticosus at 55%, 75%, and 90% shading treatment. As results, the ratio of above-ground/under-ground biomass was increased at 75% shaded condition and showed highest dry biomass. Under shaded conditions, plants had lower chlorophyll a+b content and a/b ratio and also showed thinner leave. But shaded plants showed higher leaf area and higher total leaf area per a plant. This apparently indicates adapted responses to shaded treatment. Effects of shading treatments on photosynthetic activity were higher in apparent quantum yield, carboxylation efficiency, re-phosphrylation but lower in light compensation point. These results suggested that higher photosynthesis rates in shaded treatments were due to activated carboxylation efficiency. Shading treatment had lower water use efficiency thatn controls but still higher than other tree species.

Differences in Growth and Photosynthesis among Three Half-sib Families of Betula schmidtii in Response to Cd Treatment (카드뮴 처리에 대한 박달나무의 가계간 생장과 광합성 차이)

  • 오창영;이경준;이재천;한심희
    • The Korean Journal of Ecology
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    • v.27 no.3
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    • pp.147-153
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    • 2004
  • The objective of this study was to understand differences among three half-sib families of Betula schmidtii seedlings in the effect of three Cd levels on growth and photosynthesis. One-year-old seedlings of B. schmidtii were treated with 0, 0.4 and 0.8 mM CdSO$_4$ㆍ8/3$H_2O$ for two months. Growth and physiological responses to Cd treatment levels of three families determined using dry weight, relative height growth rate(RHGR), apparent quantum yield and carboxylation efficiency. The B. schmidtii seedlings exposed to 0.4 and 0.8 mM Cd showed statisticall significant decrease in dry weight and RHGR relative to controls. In addition, the growth inhibition of B. schmidtii seedlings was accompanied by a significant decrease in net photosynthesis measured as $CO_2$ assimilation. Apparent quantum yield and carboxylation efficiency were also affected by Cd treatment, undergoing a significant and progressive reduction with increasing Cd concentrations in all families. We also found significant difference among three families of B. schmidtii in growth, biomass and photosynthesis when exposed to Cd stress. Therefore the present study showed that the difference in Cd tolerance among families might be attributed to genetic factor in response to Cd stress.

Oxidation and Removal of NO Emission from Ship Using Hydrogen Peroxide Photolysis (과산화수소 광분해를 이용한 선박 배가스 내 NO 산화흡수에 관한 연구)

  • Lee, Jae-Hwa;Kim, Bong-Jun;Jeon, Soo-Bin;Cho, Joon-Hyung;Kang, Min-Kyoung;Oh, Kwang-Joong
    • Clean Technology
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    • v.23 no.3
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    • pp.294-301
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    • 2017
  • Air pollution associated with the $NO_x$ emission from the ship engines is becoming one of the major environmental concerns these days. As the regulations on ship pollutants are strengthened, the wet absorption method, for controlling complex pollutants in a confined space, has the advantage of simultaneously removing various pollutants, but the low solubility of nitrogen monoxide is drawback. In this study, for improving existing denitrification scrubber system, NO oxidation process by hydroxyl radical produced from irradiating UV light on $H_2O_2$ is suggested and the $H_2O_2$ decomposition rates and hydroxyl radical quantum yields were measured to find the optimum condition of $H_2O_2$ photolysis reaction. As a result, the optimum quantum yield and photolysis rate of $H_2O_2$ were 0.8798, $0.6mol\;h^{-1}$ at 8 W, 2 M condition, and oxidation efficiency of 1000 ppm NO gas was 40%. In batch system, NO removal efficiency has a range of 65.0 ~ 67.3% according to input gas concentration of 100 ~ 1500 ppm. This results indicate that the scrubber system using hydrogen peroxide photolysis can be applied as air pollution prevention facility of ship engines.

Physiological responses to drought stress of transgenic Chinese cabbage expressing Arabidopsis H+-pyrophosphatase (애기장대 H+-pyrophosphatase 발현 형질전환 배추의 건조스트레스에 대한 생리적 반응)

  • Jeong, Mihye;Kang, In-Kyu;Kim, Chang Kil;Park, Kyung Il;Choi, Cheol;Han, Jeung-Sul
    • Journal of Plant Biotechnology
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    • v.40 no.3
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    • pp.156-162
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    • 2013
  • Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis $H^+$-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and $H_2O_2-3,3^{\prime}$-diaminobenzidine content when compared to wild type plants.

Efficient Triplet-triplet Annihilation-based Upconversion in Vegetable Oils (식물성 오일에서 구현되는 삼중항-삼중항 소멸법에 의한 Upconversion 분석)

  • Shin, Sung Ju;Choe, Hyun Seok;Park, Eun-Kyoung;Kyu, Hyun;Han, Sangil;Kim, Jae Hyuk
    • Applied Chemistry for Engineering
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    • v.27 no.6
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    • pp.639-645
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    • 2016
  • We herein report efficient triplet-triplet annihilation upconversion (TTA-UC) achieved in various non-toxic and non-volatile vegetable oils as a UC media using platinum-octaethylporphyrin (PtOEP) and 9,10-diphenylanthracene (DPA) as a sensitizer and acceptor, respectively. Green-to-blue UC was readily achieved from PtOEP/DPA solution in vegetable oils with the quantum yield of 8% without any deoxygenation process. The UC efficiency was found to be significantly dependent on the contents of unsaturated hydrocarbon in vegetable oils and viscosity of the solution, as well. Though the Stern-volmer constant and quantum yield in vegetable oils were measured to be lower than those measured in the deaerated organic solvent, the quenching efficiency was still high enough to be 93%. In the sunflower oil, the UC threshold intensity ($I_{th}$) was approx. $100mW/cm^2$, which is far larger than the sunlight intensity, but we believe that the UC achieved in non-toxic and air-saturated media was still highly applicable to nontraditional visualization techniques such as bioimaging.