• 생약학회지
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• 제51권4호
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• pp.332-339
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• 2020

Lentinus edodes (LE) is the first medicinal macrofungus to enter the realm of modern biotechnology. Lentinus edodes is well-known to have anti-tumor, anti-inflammatory, and immunomodulatory effects. In this study, osteoporosis was induced by ovariectomy in female rats, and the prevention and treatment efficacy of the climacteric disease the postmenopausal type I pattern was examined using the experimental substance. Female rats were either sham-operated (sham; n = 5) or surgically ovariectomized (OVX; five animals per group) and then administered to OVX control, raloxifene hydrochloride 1 mg/kg/day, or LE (20 and 200 mg/kg/day) for 12 weeks. LE treatment suppressed the ovariectomy-induced reduction of bone mineral density in the entire tibia as well as its metaphysis with a decrease of serum osteocalcin and interleukin-6 levels. In addition, LE is suggested to elevate the serum levels of progesterone hormones and prevent bone loss in ovariectomized rats. In conclusion, LE 200 mg/kg/day may have inhibitory effects on osteoporosis in OVX rats.

• 생약학회지
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• 제52권3호
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• pp.192-201
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• 2021

Dangguisu-san (DGSS) is widely known traditional herbal medicinal formula in Korea for treatment of traumatic injury by traffic accident, ecchymosis, abdominal distension and anti-thrombosis of blood. This study was conducted to develop the simultaneous analyze method using high performance liquid chromatography (HPLC) and examine the effect of anti-inflammatory activity of DGSS-dry extract (DGSS-DE) and DGSS-mix extract powder (DGSS-MEP). Physicochemical characteristics of DGSS-DE and DGSS-MEP showed that there is no significant difference in pH, titratable acidity, total soluble solid content and browning degree except for color value (L, a, b). 15 functional constituents of DGSS were identified and the correlation coefficient values of DGSS-DE and DGSS-MEP were conformed 0.950. Also, DGSS-DE and DGSS-MEP significantly decreased the secretion of nitric oxide (NO), prostaglandin E₂ (PGE₂), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) through inhibited expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, IL-6, and TNF-α. From these result, DGSS-MEP showed similar chemical composition and anti-inflammatory effect to DGSS-DE. Therefore, DGSS-DE and DGSS-MEP may be useful as potential source of drug to prevent inflammation.

• 한국산업안전학회:학술대회논문집
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• 한국안전학회 2000년도 춘계 학술논문발표회 논문집
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• pp.223-228
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• 2000

지진격리교량은 강진지역의 지반운동과 구조물의 거동에 근거한 내진설계 개념을 바탕으로 설계되었다. 그러나 지반운동의 특성과 구조물의 거동에 있어서 한반도와 같은 중약진 지역과 지각변동운동이 활발한 강진지역은 큰 차이를 보이고 있다. 따라서 중약진 지역의 내진설계에서는 지반운동의 특성이 반영되어야 할 필요가 있으며, 특히, 지진격리교량의 경우에는 교각의 연성 등 중약진 지역의 구조물의 거동특성이 반영되어야 한다. (중략)

• 한국약용작물학회지
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• 제28권6호
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• pp.471-480
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• 2020

Background: The roots of Cirsium japonicum var. ussuriense (RCJ) have been used as traditional medicine in Korea for hematuria and hematemesis. These extracts exert anti-oxidative and anti-inflammatory effects by scavenging for free radical and regulating the inflammatory response. However, the effect of RCJ on rheumatoid arthritis (RA) has not been elucidated. Thus, we evaluated the water extract of RCJ (WRCJ) using type II collagen-induced RA models. Methods and Results: RA was induced by immunization with type II collagen. All experimental materials were orally administered daily for three weeks. The positive control group was administered with 0.2 mg/kg methotrexate (n = 7), while the experimental group was administered with WRCJ (100 or 500 mg/kg, n = 7). Serum levels of TNF-alpha, Interleukin 6 (IL-6), and type II collagen IgG (CII) were measured using ELISA. Administration of 500 mg/kg WRCJ decreased the levels of TNF-alpha, IL-6, and CII. Moreover, WRCJ treatment diminished swelling of hind legs and infiltration of inflammatory cells in RA models' synovial membrane. Conclusions: These results indicate that WRCJ could improve RA, reduce inflammatory indicators and synovial inflammation. However, further experiments are required to determine how WRCJ can influence the signal transduction pathway in RA.

• 한국지진공학회논문집
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• 제10권3호
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• pp.57-64
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• 2006

현행 약진지역의 내진설계기준은 주로 강진지역에서의 연구결과에 근거하고 있다. 하지만, 약진지역의 경우 지진하중보다는 중력하중이나 풍하중에 의해 구조설계가 지배되므로 구조물의 초과강도가 강진지역의 경우보다 증가하게 된다. 따라서 약진지역에 적합한 내진설계기준을 마련하기 위해서는 강진지역에 적용되는 반응수정계수를 약진지역에 그대로 적용할 수 있는지에 대한 검증이 필요하다. 본 연구에서는 건축구조물에 대한 소성해석을 통해 그 연성도와 초과강도를 산정하고 이에 근거하여 현행 반응수정계수의 적절성 여부를 검토하였다. 강진, 중진, 약진지역 등에서의 초과강도와 연성요구도를 비교하기 위하여 UBC-97에 근거하여 설계된 예제구조물을 선정하여 해석을 수행하였다. 해석결과에 의하면 약진지역의 초과강도가 강진지역보다 크기 때문에 동일한 반응수정계수에 대한 약진지역의 연성요구도는 강진지역에서보다 적게 된다. 따라서 동일한 반응수정계수를 이용하여 설계된 약진지역 구조물의 경우 접합부에서의 소성회전각 요구량을 강진지역의 경우에 비하여 상대적으로 저감시킬 수 있을 것이다.

• 한국자원식물학회지
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• 제32권4호
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• pp.282-289
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• 2019

본 연구는 에탄올과 염산으로 유도된 위염모델에 대해 익모초의 물 추출물이 위염예방효과를 나타내는지 알아보기 위해 진행하였고, 안전성 평가를 위해 유전독성평가인 소핵시험을 수행하였다. 익모초 물 추출물은 음성대조군 대비 위에서 분비되는 $PGE_2$의 농도를 증가시켰을 뿐만 아니라 염산과 에탄올 투여에 의한 점막 표피세포 및 선상피세포의 손상과 울혈을 충분히 예방하는 것을 확인하였다. 또한 익모초 물 추출물에 대한 소핵시험을 실시한 결과, 익모초 물 추출물은 소핵을 유발하지 않는 것으로 나타났다. 결과를 종합하였을 때 익모초 물 추출물은 자체적으로 위염 예방효과를 나타냈지만 정확히 어떤 기전을 통해 예방하는지 추가적인 실험이 필요하다고 사료되며, 익모초에 포함된 여러 단일 성분을 이용해 위염 예방평가를 실시하여, 위염에 대한 익모초의 효능과 효율을 높이는 연구가 필요하다고 생각된다.

• 대한본초학회지
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• 제34권6호
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• pp.57-62
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• 2019

Objective : Gastritis is a major complication of gastrointestinal disease. Lonicera japonica is used in folk medicine to treat different diseases such as exopathogenic wind-heat, epidemic febrile diseases, sores, carbuncles and some infectious diseases. Therefore, this study examined the effects of Lonicera japonica water extract (LJE) on HCl/ethano-linduced acute gastric ulceration and anti-oxidants properties. Methods : LC-ESI-IT-TOF MS was employed for rapid identification of major compound from LJE. The antioxidant activities were evaluated through total polyphenol and flavonoid contents and radical scavenging assays and superoxide dismutase (SOD)-like activity. SD rats were randomly divided into five different groups including the normal group, ulcer group, positive group (20 kg/mg of omeprazole, ip), and experimental groups (100 kg/mg and 500 kg/mg of LJE, ip). Results : 4,5-Dicaffeoyl quinic acid, loganic acid, secologanic acid, sweroside, loganin, vogeloside were identified based on the detection of the molecular ion with those of literature data. The LJE was possessed free radical scavenging activities such as DPPH (IC₅₀=189.7 ㎍/㎖), ABTS (IC₅₀=164.5 ㎍/㎖), and SOD-like activity (IC₅₀=405.02 ㎍/㎖). Macroscopic and histological analyses showed LJE treated group were significantly reduced to an extent that it allowed leukocytes penetration of the gastric walls compared with the ulcer group. In addition, an ulcer inhibition rate and prostaglandin E₂ levels were increased in rats treated with LJE. Conclusion : The present study has demonstrated the antioxidantive and gastroprotective effect of LJE, these findings suggested that LJE has the potential for use in treatment of gastric disorders.

• 대한본초학회지
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• 제36권1호
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• pp.67-76
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• 2021

Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development. Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 mg/kg) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment. Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured. Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines. Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.

• 대한본초학회지
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• 제39권2호
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• pp.19-25
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• 2024

Objectives : Jeopgoltang (JGT) is a new Korean herbal medicine formulation that is used to treat bone fractures. Although JGT is frequently used in clinical practice, there is a lack of scientific evidence on its safety. This study aimed to evaluate the preclinical toxicity of JGT using a single oral dose toxicity test in Sprague-Dawley (SD) rats. Methods : Five male and female rats per group were orally administered 1,250, 2,500, or 5,000 mg/kg of JGT after fasting for 12 h. Mortality and changes in clinical signs, body weight, and necropsy findings were monitored for 14 days according to the guidelines of the Korean Ministry of Food and Drug Safety and Organisation for Economic Co-operation and Development (OECD). Results : No significant clinical signs or mortality were observed after a single administration of up to 5,000 mg/kg. In addition, no significant necropsy findings related to JGT administration were observed. Conclusions: In conclusion, these results suggest that approximate Lethal Dose (ALD) of JGT on SD rats is over 5,000 mg/kg.

• 한국지진공학회논문집
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• 제5권2호
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• pp.59-66
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• 2001

구조물 내진설계의 개념은 기존요구조건이라는 조항으로 시방서에 규정되어 있으며 구조물이 지진발생시에 안전성과 경제성을 최대한 확보할 수 있으며 비선형시간 이력해석을 수행하여 자진시의 동적거동을 기술함으로써 확인할 수 있다. 내진설계에 보편적으로 적용하는 응답스펙트럼해석법은 선형해석법으로 구조물의 비선형동적거동의 영향을 거동계수로 반영하므로 파괴메카니즘 및 기본 요구조건의 만족여부를 거동계수를 구하는 과정으로 결정할 수 있다. 이 연구에서는 내진설계방식에 의해 설계된 약진지역에 의한 화학공장건물의 모델인 3차원 철골뼈대구조물을 선정하고 거동계수를 결정하는 과정을 수행하여 지진시의 동적거동을 확인하였다. 이 연구의 결과, 현 시방서의 응답스펙트럼해석법에 적용되는 거동계수는 강진지역의 구조물의 경우 기능성 및 안정성 한계를 제시하지만 약진지역 구조물의 경우는 실제 동적거동과 무관하다는것과 약진 지역에 위치한 구조물의 내진설계에는 시방서가 제시한 내진설계방식을 적용하는 것이 주요한 사항임을 확인하였다.