• Proceedings of the KSME Conference
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• 2001.06b
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• pp.670-675
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• 2001

This paper is to propose image segmentation method based on chamfer algorithm. First, we get original image from CCD camera and transform it into gray image. Second, we extract maximum gray value of background and reconstruct and eliminate the background using surface fitting method and bilinear interpolation. Third, we subtract the reconstructed background from gray image to remove noises in gray image. Fourth, we transform the subtracted image into binary image using Otsu's optimal thresholding method. Fifth, we use morphological filters such as areaopen, opening, filling filter etc. to remove noises and isolated points. Sixth, we use chamfer distance or Euclidean distance to this filtered image. Finally, we use watershed algorithm and count microorganisms in image by labeling. To prove the effectiveness, we apply the proposed algorithm to one of Ammonia-oxidizing bacteria, Acinetobacter sp. It is shown that both Euclidean algorithm and chamfer algorithm show over-segmentation. But Chamfer algorithm shows less over-segmentation than Euclidean algorithm.