• Applied Microscopy
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• v.31 no.2
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• pp.167-173
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• 2001

This study, the ultrastructure of radial glial cells in the fetuses (the 60th, 90th, 105th 120th of gestation) and neonate brains of korean native goat were investigated by immunohistochemical method and transmisson electron microscopy. 1. In the 60th day of gestation, mitochondria and many glycogen body were observed in the radial glial cells. 2. In the 90th day of gestation, mitochondria, many glycogen body and rough endoplasmic reticulum were observed. 3. In the 95th day of gestation, Golgi's apparatus was found. 4. In the 120th day of gestation, Endfeets of radial glial cell attached vessel wall were observed.

• Journal of Conservation Science
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• v.34 no.5
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• pp.397-405
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• 2018

Currently, there are very few studies on Korean wall paintings. Therefore, this study discusses the current conditions of wooden paintings and the characteristics of the adhesive agent in the painting layer separation. Korean land pine was chosen as the support, while white oysters shells, orpiment, red ocher, Noerok, and azurite were used as pigments. With four adhesive agents, including animal glue, Gelidium, methyl cellulose, and PVAc (caparol binder), a comparative experiment was conducted, by dividing them into two concentrations, of 0.5% and 1.5%. The temperature, humidity, and ultraviolet rays, which are contributing environmental factors in cultural assets after fixing, were artificially investigated. After deterioration, observed color difference, fixing, and the surface. Results showed that the animal glue strongly fixing all the colored layers compared to the other adhesives; however, azurite had a partial change when used outdoors. With Gelidium, which functioned similar to animal glue, the azurite was affected by the ultraviolet rays; nevertheless, despite the variations in temperature and humidity, it had the best gripping force compared to the other adhesive agents. Methyl cellulose was glossy at a high concentration, and was relatively strong against rapid changes in temperature and humidity. PVAc significantly reduced the binding force, compared to other adhesive agents.

• Korean Journal of Environmental Agriculture
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• v.26 no.2
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• pp.149-158
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• 2007

The objective of this study were to offer a basis data for application of management of forest resources through grasping of species composition by layer of forest vegetation in the Mt. Geumwon recreational forest. The tree and subtree layer consisted of 27 and 44 species in Mt. Geumwon recreational forest respectively. The importance value of Quercus mongolica (57.1) and Q. serrata (40.4) were highest in the tree layer and that of Acer pseudo-sieboldianum (43.4) was highest in the subtree layer, respectively. The shrub and herb layer was composed of 71 and 69 species, respectively. The value of species diversity ranged from 1.0391 to 1.5403. Evenness ranged from 0.7259 to 0.8320. Dominance was 0.2741 at tree layer, 0.2440 at subtree layer, 0.1680 at shrub layer, and 0.1702 at herb layer, which showed that Mt. Geumwon recreational forest is dominated by a large number of species at all layer. The result of forest vegetation in Mt. Geumwon recreational forest may be utilized as a basic data for application of reasonable ecological management of forest resources.

• Journal of agriculture & life science
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• v.46 no.5
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• pp.1-10
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• 2012

This study analyzed on the characteristics of vegetation structure, species composition and DBH class distribution in order to conservation and effective management for Taxus cuspidata community in Mt. Seorak, Mt. Balwang, Mt. Taebaek, and Mt. Odae. The vegetation in upper, subtree and shrub layer was consist of 11, 22, 33 species in Mt. Seorak, 15, 21, 33 species in Mt. Balwang, 10, 23, 36 species in Mt. Taebaek, and 14, 30, 32 species in Mt. Odae. As a result of importance value at all study sites, T. cuspidata and Abies nephrolepis in upper layer, T. cuspidata, A. nephrolepis and Acer komarovii in subtree layer, and Tripterygium regelii in shrub layer were high, respectively. Species diversity in upper and subtree layer at all study sited were ranged 0.834~1.234 and 1.125~1.329, respectively. According to the DBH class of major three species, T. cuspidata in Mt. Odae site showed a reverse J-shaped curve, which was estimated that T. cuspidata community of this site might be maintained continuously as a stable state.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2015.11a
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• pp.222-223
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• 2015

주석 폐자원에서 전해정련을 통해 고순도 주석을 회수하고자 전해정련에 대한 연구를 수행하였다. 실험결과 전해정련으로는 Pb와 Sn을 분리 회수가 어려우나 전해액의 주석 농도를 25g/L이상으로 유지하고, 황산 첨가를 통하여 전해 주석의 Pb함량 제어가 가능함을 알 수 있었다. 전해를 위한 인가전압은 0.2V에서 고순도의 주석을 얻을 수 있었고, 안정적인 전해정련 공정을 수행하기 위해서는 첨가제로 베타나프톨과 아교가 필요하며 각각 0.1%, 1%가 적정 조건임을 알 수 있었다.

• Korean Journal of Environment and Ecology
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• v.9 no.2
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• pp.221-231
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• 1996

The planting plan for the establishment of Eco-bridge were suggested to restore the ecosystem where has been ecologically isolated by rood construction in Chimgogae of Odaesan National Park. Eco-bridge contributes as a corridors to the faunal movement. To survey the vegetation, 16 plots within 6 sites were selected and 10 by 10 meters in size set up. In the results of Chingogae's vegetation analysis, succession trends was from Quercus mongolica to Carpinus laxiflora, Carpinus cordata, Fraxinus rhynchophylla, Tilia amurensis and so on. It turned out that the nearer sites had high similariyt and vegetation continuity obviously in the similarity index analysis. In the analysis of the number of species and individuals, final step of planting plan needs about 15 species and 10~12 trees, 17~18 subtrees and 100 shrubs in size 100m$^{2}$. Planting species was selected from adjacent sites. The plant species suited for the eco-bridge establishment in this area can be suggested such as Carpinus cordata, Cornus controversa, Q. mongolica etc. and 12 subtree and shrub such as Syringa reticulata var. mandshurica, Sambucus williacsii var. coreaca, Acer pseudosieboldianum and so on.

• Korean Journal of Microbiology
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• v.46 no.1
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• pp.93-95
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• 2010

White rot fungi which have lignin degrading enzymes show high degrading activity to diverse recalcitrant compounds such as polycyclic aromatic compounds, dyes, explosives and endocrine disrupting chemicals. We have examined decolorizing activity of dyes by Phlebia tremellosa and two transformants which had genetically transformed using laccase or manganese peroxidase (MnP) gene. In case of methyl green, wild type strain showed 50% decolorization while laccase transformant (TF2-1) and MnP transformant (T5) showed more than 90% decolorization on day 3. Remazol brilliant blue R(RBBR) was decolorized up to 85% by two transformants while the wild type showed 67% decolorization on day 3. Transformants TF2-1 and T5 both showed increased laccase and MnP activity respectively during the whole growing phase.

• The Journal of the Korea Contents Association
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• v.17 no.4
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• pp.520-531
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• 2017

The purpose of this study was to test the validity and reliability of the Korean version of the Tuberculosis Interview Instrument (K-TII). The instrument was first translated into Korean, back-translated to English, and reviewed. After the translation process, the instrument was revised from 52 items to 37 items by researchers and an expert according to the systematic review. In addition, these items were reduced to 32 items after the content validity test was done by 9 experts. As a result of pilot study conducted in 20 early childhood teachers, 32 items were selected for the preliminary instrument. Data collected from 289 early childhood teachers were used to test the validity and reliability of the preliminary instrument. The result of factor analysis showed that the instrument categorized into 7 factors explaining 71% of the total variance and 27 items were retained for the final instrument. The result of Cronbach's ${\alpha}$ coefficient was .88. In conclusion, the K-TII may be a suitable instrument for assessing tuberculosis prevention behaviors in early childhood teachers in Korea.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.3
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• pp.285-296
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• 1997

Injury to brain transforms resting astrocytes to their reactive form, the hallmark of which is an increase in glial fibrillary acidic protein (GFAP), the major intermediate filament protein of their cell type. The overall glial response after brain injury is referred to as reactive gliosis. Glial-neuronal interaction is important for neuronal migration, neurite outgrowth and axonal guidance during ontogenic development. Although much attention has been given to glial regulation of neuronal development and regeneration, evidences also suggest a neuronal influence on glial cell differentiation, maturation and function. The aim of the present study was to analyze the effects of glial-hippocampal neuronal co-culture on GFAP expression in the co-cultured astrocytes. The following antibodies were used for double immunostaining chemistry; mouse monoclonal antibodies for confirm neuronal cells, rabbit anti GFAP antibodies for confirm astrocytes. Primary cultured astrocytes showed the typical flat polygonal morphology in culture and expressed strong GFAP and vimentin. Co-cultured hippocampal neurons on astrocytes had phase bright cell body and well branched neurites. About half of co-cultured astrocytes expressed negative or weak GFAP and vimentin. After 2 hour glutamate (0.5 mM) exposure of glial-neuronal co-culture, neuronal cells lost their neurites and most of astrocytes expressed strong CFAE and vimentin. In Western blot analysis, total GFAP and vimentin contents in co-cultured astrocytes were lower than those of primary cultured astrocytes. After glutamate exposure of glial-neuronal co-culture, GFAP and vimentin contents in astrocytes were increased to the level of primary cultured astrocytes. These results suggest that neuronal cell decrease GFAP expression in co-cultured astrocytes and hippocampal neuronal-glial co-culture can be used as a reactive gliosis model in vitro for studying GFAP expression of astrocytes.

• Korean Journal of Microbiology
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• v.48 no.3
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• pp.225-227
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• 2012

Lignin degrading enzymes from white rot fungi show broad substrate specificities, and therefore they can degrade variety of recalcitrant compounds. We have used three different protocols for the generation of immobilized laccase and manganese peroxidase crude enzymes from the genetically transformed strains of Merulius tremellosus Fr. These immobilized enzymes were used in the decolorization of Remazol Brilliant Blue R (RBBR), and they showed about 75% decolorization rates during the 48 h reactions. Although the decolorization efficiency decreased by 10-15% after a repeated use of the immobilized enzymes, these can be reused in various degrading reactions.