• Proceedings of the Korea Information Processing Society Conference
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• 2018.10a
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• pp.603-605
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• 2018

최근 대두되고 있는 딥 러닝은 학습을 통해 사물이나 데이터를 군집화하거나 분류하는 데 사용하는 기술이다. 본 논문은 딥 러닝에 활용하기 위해 개발된 오픈소스 소프트웨어인 텐서플로 Inception V3을 사용해 연구를 진행했다. 딥 러닝을 활용한 씨앗 발아 확인 시스템은 기존의 영상 처리를 활용한 시스템에서 고안했으며, 씨앗 발아 여부의 정확성이 떨어지는 단점을 개선하고, 모든 종자들의 발아 여부를 확인할 수 있도록 구현해 사용자가 효과적으로 연구를 수행할 수 있도록 하는 목적에 있다.

• Proceedings of the Korea Information Processing Society Conference
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• 2018.05a
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• pp.212-214
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• 2018

식물 연구 분야에서 진행하는 씨앗 발아 확인실험에 대한 사용자의 편의성을 증대시키기 위한 씨앗발아 확인 시스템을 구현하는데 목적이 있다. 본 시스템을 구현 하여 기존 수작업으로 진행하던 실험을 자동화하여 인력 및 시간 소모를 줄여 작업 능률 개선 측면에서 기대효과가 있다.

• Applied Biological Chemistry
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• v.39 no.1
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• pp.25-31
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• 1996

Transgenic tobacco plants expressing calmodulin derivative($lys{\rightarrow}ile$ 115 calmodulin) and hygromycin resistance genes or hygromycin resistance gene alone(control) were generated by Agrobacterium-mediated DNA transfer. Seeds obtained from the transgenic plants($F_o$) were tested for resistance to hygromycin and the expected 3 : 1 ratio was observed. The expression of calmodulin derivative in the tobacco plants was identified by a combined method of Western blot and Chemiluminescence. The effects of surface sterilizers on the germiation of seeds from transgenic tobacco plants were tested in Murashige and Skoog agar medium. Seeds obtained from transgenic tobacoo plants expressing the calmodulin derivative showed no fungi contamination with normal germination by treating with sterilized water alone or sodium hypochlorite(2% effective chlorine). In contrast, seeds from the control transgenic tobacco plants showed severe contamination with fungi by treating with sterilized water alone and showed no contamination with normal germination by treating with sodium hypochlorite(2% chlorine). The effects of calcium concentration on the germination of seeds from transgenic tobacco plants were tested in Murashige and Skoog agar medium. Seeds obtained from transgenic tobacco plants expressing the calmodulin derivative showed better germination frequency than that of the control transgenic tobacco seeds in the medium containing 30 mM $CaCl_2$. The data raise the possibility that the expression of calmodulin derivative gene in tobacco plants could increase adaptability of the seeds to environmental stresses.

• Journal of Ginseng Research
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• v.33 no.2
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• pp.139-142
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• 2009

The effect of calcium cyanamide (China-made) soil fumigation on the growth of the ginseng pathogen Rhizoctonia, Pythium), soil microbes, and seed germination of lettuce and radish was investigated. At twice the recommended level (2S0-ppm $CaCN_2$), the growth of Rhizoctonia and Pythium, and the seed germination, were not inhibited. Rather, the effective level was 10,000 ppm. The powder form was more effective than the granular form in inhibiting pathogen growth and seed germination. The lettuce seed was also more sensitive than the radish seed. Calcium cyanamide appearedto decrease the fungi population and to increase Actinomycetes in the soil.

• Journal of Life Science
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• v.31 no.8
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• pp.778-785
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• 2021

The germination of cucumber seeds begins with the degradation of reserved oil to fatty acids within the lipid body, which are then further metabolized to acyl-CoA. The acyl-CoA moves from the lipid body to the glyoxysome following β-oxidation for the production of acetyl-CoA. As an initial carbon source supplier, acetyl-CoA is an essential molecule in the glyoxylate cycle within the glyoxysome, which produces the metabolic intermediates of citrate and malate, among others. The glyoxylate cycle is a necessary metabolic pathway for oil seed plant germination because it produces the metabolic intermediates for the tricarboxylic acid (TCA) cycle and for gluconeogenesis, such as the oxaloacetate, which moves to the cytosol for the initiation of gluconeogenesis by phophoenolpyruvate carboxykinase (PEPCK). Following reserved oil mobilization, the production and transport of various metabolic intermediates are involved in the coordinated operation and activation of multiple metabolic pathways to supply directly usable carbohydrate in the form of glucose. Furthermore, corresponding gene expression regulation compatibly transforms the microbody to glyoxysome, which contains the organelle-specific malate synthase (MS) and isocitrate lyase (ICL) enzymes during oil seed germination. Together with glyoxylate cycle, carnitine, which mediates the supplementary route of the acetyl-CoA transport mechanism via the mitochondrial BOU (A BOUT DE SOUFFLE) system, possibly plays a secondary role in lipid metabolism for enhanced plant development.