• Title/Summary/Keyword: 신해충

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Effect of several new insecticides to the bamboo-shoot cutworm, Bambusiphila vulgaris BUTLER (죽순나방(Bambusiphila vulgaris)에 대한 신살충제의 효과)

  • 윤주경
    • Korean journal of applied entomology
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    • v.2
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    • pp.44-46
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    • 1963
  • 1). To find the effective insecticides and its timing for the control of the bamboo-shoot cutworm, Bambusiphila vulgaris BUTLE R, this experiment was carried out at Tam-Yang in Chula-namdo from May to June in 1963 in Korea. 2). Endrin, Folidol, and Diazinon were more effective than sumithion, Aldrin, and EPN. .B). The best timing of insecticidal spraying was in 5 days after hatching front the eggs. 4). Since this experiment is the result only tried just once, I hope that the same experiment should be repeated several times and consequently effective of killing insects and the best timing for sprinkling insecticides should be also Investigated.

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Qualitative and quantitative PCR detection of insect-resistant genetically modified rice Agb0101 developed in korea (해충저항성 유전자변형 벼 Agb0101에 대한 PCR 검정)

  • Shin, Kong-Sik;Lee, Jin-Hyoung;Lim, Myung-Ho;Woo, Hee-Jong;Qin, Yang;Suh, Seok-Cheol;Kweon, Soon-Jong;Cho, Hyun-Suk
    • Journal of Plant Biotechnology
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    • v.40 no.1
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    • pp.18-26
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    • 2013
  • Genetically modified (GM) rice Agb0101, which expresses the insecticidal toxin modified cry1Ac (mcry1Ac1) gene, was developed by the Rural Development Administration in Korea. To monitor the probable release of Agb0101 in the future, it is necessary to develop a reliable detection method. Here, we developed the PCR detection method for monitoring and tracing of GM rice. The primer pair (RBEgh-1/-2) from a starch branching enzyme (RBE4) gene was designed as an endogenous reference, giving rise to an expected PCR amplicon of 101 bp. For the qualitative PCR detection, construct- and event-specific primers were designed on the basis of integration sequence of T-DNA. Event-specific PCRs amplified specifically 5'- or 3'-junction region spanning the native genome DNA and the integrated gene construct, while none of amplified product was shown on crops, rice varieties, and other insect-resistant transgenic rice lines. The event-specific real-time PCR method was performed using TaqMan probe and plasmid pRBECrR containing both rice endogenous gene RBE4 sequence and 5'-junction sequence as the reference molecule. The absolute limit of quantification (LOQ) of real-time PCR was established with around 10 copies for one plasmid molecule pRBECrR. Thereafter, the different amounts of transgenic rice (1, 3, 5, and 10%, respectively) were quantified by using the established real-time PCR method, with a range below 19.55% of the accuracy expressed as bias, 0.06-0.40 of standard deviation (SD) and 3.80-7.01% of relative standard deviations (RSD), respectively. These results indicate that the qualitative and quantitative PCR methods could be used effectively to detect the event Agb0101 in monitoring and traceability.

Cystic Diseases of the Kidney in Children (소아의 낭포성 신질환)

  • Lee Ji-Suk;Rho Kwang-Sik;Kim Ji-Hong;Lee Jae-Seung;Kim Pyung-Kil
    • Childhood Kidney Diseases
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    • v.1 no.2
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    • pp.144-150
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    • 1997
  • Purpose : The kidney is one of the most common sites of cyst formation. Cystic diseases of the kidney are a diverse group of clinicopathologic entities and variable prognosis. They span a wide range of both age of presentation and severity of the renal disease. And many of them are systemic disorders, sharing similar process of cyst formation in other organs. Recently, development of imaging studies has been contributing widely to the diagnosis of the diseases. Treatment, however, is not established satisfactorily. We performed this study to evaluate the occurrence and treatment of cystic diseases of the kidney. Methods : We reviewed retrospectively the medical records of 44 patients with cystic diseases of the kidney in the Department of Pediatrics, during last 11 years. Results : In the 44 patients with cystic diseases of the kidney, 31 patients(71%) had multicystic dysplastic kidney and 11(35%) of them received nephrectomy due to differentiation from neoplasms or severe abdominal distension. Seven patients(16%) had polycystic kidney disease, and all of them were infantile type. Five patients(11%) were diagnosed as having a simple renal cysts. Progression to renal failure was noted in none of the cases. In 14(32%) out of total 44 patients, the diagnosis was made in neonatal or infantile pelted. Conclusion : The incidence of cystic diseases of the kidney appeared very low, but further investigation on their pathogenesis, classification, and indication of treatment is needed.

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A Study on the Manufacturing Method of Natural Stretch Yarns using Nylon 66 (Nylon 66를 이용한 Natural Stretch Yarns 제조방법에 관한 연구)

  • Park, Seong-Woo;Seo, Mal-Yong;Hong, Sang-Ki;Choi, Hae-Chung;Choi, Bo-Yun
    • Proceedings of the Korean Society of Dyers and Finishers Conference
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    • 2011.03a
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    • pp.96-96
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    • 2011
  • 본 연구에서는 스포츠 캐주얼 웨어(스노우보드복, 골프복, 등산복 등)로 사용되는 신축성 소재의 개발로 Stretch성을 부여하기 위하여 Nylon 66 POY 소재의 가연가공 기술을 통하여 Mechanical Stretch 성능이 발현되는 소재를 개발하고자 한다. 섬유소재에 일반적으로 신축성을 부여하기 위해서는 Spandex(Polyurethane, PU)를 방적공정에서 복합 제조하는 Core Yarn과 장섬유에 Spandex를 피복하는 Covering Yarn(직물용) 형태가 있는데, Spandex로 인한 신축성은 200~800% 발현되나 PU를 사용함에 따라서 공정추가에 대한 원가상승, 균제도 불량, 몰림현상 및 PU 수축에 의한 중량감이 있으며, 또한 PU에 의한 견뢰도불량, 피복사의 벗겨짐성 등 외관상 트러블 발생빈도가 높다. 이에 따라 Spandex를 사용하지 않고 단일소재로서 사가공기술에 의해 Stretch성이 부여되는 소재를 개발하고 이의 기술을 상용화 하고자 한다. Nylon 66 POY 소재를 Disc 가연기, Pin 가연기 등의 설비를 이용하여 70d급 원사의 가연가공 공정을 진행하였다. 그리고 개발된 원사의 섬도(d), 강도(g/d), 신도(%), 크림프율(%)의 물성 시험을 통하여 Nylon66의 공정상 변화인자에 따른 기본 물성 및 Stretch 발현성을 분석하여 최적의 설비 및 공정조건을 확립하였다.

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Analysis of junction between T-DNA and plant genome in insect resistance GM Chinese cabbage (해충저항성 GM 배추에서 T-DNA와 식물체 게놈의 인접 부위 분석)

  • Lim, Sun-Hyung;Park, Seung-Hye;Kim, Jung-Hwan;Kim, Na-Young;Won, So-Youn;Lee, Si-Myung;Shin, Kong-Sik;Woo, Hee-Jong;Kim, Dong-Hern;Cho, Hyun-Suk
    • Journal of Plant Biotechnology
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    • v.35 no.2
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    • pp.101-108
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    • 2008
  • The Agrobacterium-mediated transformation has been successfully used method to introduce foreign genes into some monocotyledonous as well as a large number of dicotyledonous plants genome, We developed transgenic Chinese cabbage plants with insect-resistance gene, modified CryIAc, by Agrobacterium-transformation and confirmed transgene copy number by Southern blot analysis. We confirmed that twenty-nine out of 46 transgenic Chinese cabbage plants have single copy of CryIAc. To obtain the sequences information on the transferred DNA (T-DNA) integration into plant genome, we analyzed left border (LB) flanking sequences by genome walking (GW) PCR method. Out of 46 transgenic Chinese cabbage plants examined, 37 carried the vector backbone sequences. This result indicates that the transfer of the vector backbone from the binary vectors resulted mainly from inefficient termination of LB site. Analysis of T-DNA LB flanking region of 9 transgenic Chinese cabbage plants without vector backbone revealed that all LB ends were not conserved and nucleotides up to 36bp from the LB cleavage site were deleted.

Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice (벼물바구미 (Lissorhoptrus oryzophilus) 내충성 GM 벼에서 T-DNA와 게놈의 인접부위 분석)

  • Lee, Jin-Hyoung;Shin, Kong-Sik;Suh, Seok-Cheol;Rhim, Seong-Lyul;Lim, Myung-Ho;Woo, Hee-Jong;Qin, Yang;Kweon, Soon-Jong;Park, Soon-Ki
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.127-133
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    • 2014
  • Four transgenic rice lines harboring insect-resistant gene cry3A showed ideal field performances characterized by high considerable resistance to rice water weevil (Lissorhoptrus oryzophilus Kuschel). In this study, we estimated the insertion number of foreign genes, and analyzed the flanking sequences of T-DNAs in rice genome. As a result, T-DNA of BT12R1 line was inserted in exon region of rice chromosome 10. Two copies of T-DNAs were inserted in line BT12R2. BT12R3 line was analyzed at only left border flanking sequence. BT12R4 line was confirmed one copy of foreign gene insertion at the position 24,516,607 ~ 24,516,636 of rice chromosome 5, accompanied by a deletion of 30 bp known genomic sequences. This intergenic position was confirmed none of expressed gene and any deletion/addition of T-DNA sequence. In conclusion, these molecular data of rice water weevil resistant Bt rice would be used to conduct the biosafety and environment risk assessment for GM crop commercialization.

Effectiveness of an Environment-friendly Fumigator for Microorganisms that Can Cause Biological Damage to Records in Japan, Malaysia, and Oman (일본, 말레이시아, 오만의 기록물에서 분리한 유해미생물에 대한 친환경 기록물 소독장비 효과검증)

  • Kim, Min Ji;Lee, Eon Sook;Choi, Young Nam;Choi, Young Sin
    • Journal of Korean Society of Archives and Records Management
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    • v.15 no.2
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    • pp.165-179
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    • 2015
  • Archives have historical and academic values. That is why the study of preservation conditions and the environment for the effective management of various records progress. However, materials such as paper and adhesives will inevitably cause biological damages. These damages spread into the inner side of archives, which would make it impossible to recover. For example, archival records from Malaysia and Oman are easily exposed to biological damage because of these countries' hot and humid climate. As such, once records are damaged, disinfection operations are customary in these areas. Methyl bromide (MeBr) and ethylene oxide (EtO) were used in the disinfection process as they showed good insecticidal effect. However their antibacterial effect are negligible. As such, the need for an environmental-friendly fumigator was evident. An environmental-friendly fumigator is designed to improve storage stability. Such equipment is expected to contribute to prevention of damages to cultural heritages through its use of vaporization technology, maintenance of a disinfection space (chamber), and self-generation of nitrogen.

Drone-based smart quarantine performance research (드론 기반 스마트 방재 방안 연구)

  • Yoo, Soonduck
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.2
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    • pp.437-447
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    • 2020
  • The purpose of this study is to research the countermeasures and expected effects through the use of drones in the field of disaster prevention as a drone-based smart quarantine performance method. The environmental, market, and technological approaches to the review of the current quarantine performance task and its countermeasures are as follows. First, in terms of the environment, the effectiveness of the quarantine performance business using drone-based control is to broaden the utilization of forest, bird flu, livestock, facility areas, mosquito larvae, pests, and to simplify and provide various effective prevention systems such as AI and cholera. Second, in terms of market, the standardization of livestock and livestock quarantine laws and regulations according to the use of disinfection and quarantine missions using domestic standardized drones through the introduction of new technologies in the quarantine method, shared growth of related industries and discovery of new markets, and animal disease prevention It brings about the effect of annual budget savings. Third, the technical aspects are (1) on-site application of disinfection and prevention using multi-drone, a new form of animal disease prevention, (2) innovation in the drone industry software field, and (3) diversification of the industry with an integrated drone control / control system applicable to various markets. (4) Big data drone moving path 3D spatial information analysis precise drone traffic information ensures high flight safety, (5) Multiple drones can simultaneously auto-operate and fly, enabling low-cost, high-efficiency system deployment, (6) High precision that this was considered due to the increase in drone users by sector due to the necessity of airplane technology. This study was prepared based on literature surveys and expert opinions, and the future research field needs to prove its effectiveness based on empirical data on drone-based services. The expected effect of this study is to contribute to the active use of drones for disaster prevention work and to establish policies related to them.

Biochemical Adaptation of the Oriental Tobacco Budworm, Helicoverpa assulta, to Host-plant Defensive Compounds (기주식물 방어물질에 대한 담배나방의 생화학적 적응)

  • Ahn, Seung-Joon
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.143-154
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    • 2022
  • Plant secondary metabolites play an important role in insect-plant interactions. Herbivorous insects have various strategies to cope with the plant defensive compounds. Polyphagous insects feed on a wide variety of plant species, and their detoxification mechanisms are more complex since they tend to respond to a large array of different plant-derived chemicals. Alternatively, oligophagous insects specialize on only a few related plant species and may be expected to have a more efficient form of adaptation. This adaptation could involve either the production of large quantities of enzymes to detoxify their defensive compounds or the sequestration of the compounds or their metabolites. The oriental tobacco budworm, Helicoverpa assulta, is a specialist herbivore, feeding on a few plants of Solanaceae, such as tobacco and hot pepper. Understanding its host-plant adaptation not provides an important insight on physiology, ecology and evolution of specialist herbivores, but also gives a clue to develop management strategies of the pest species such as H. assulta. This paper briefly reviews the specialist, H. assulta, focusing on its host range, larval associations with the host plants, and detoxification mechanisms to nicotine and capsaicin, two characteristic defensive compounds derived from its two major host plants, tobacco and hot pepper, respectively. It summarizes the relevant research over the last half century and provides a future perspective on this subject.

2DEG Transport Analysis in AlGaAs/GaAs Interface by MONTE-CARLO Method (MONTE-CARLO 방법에 의한 AlGaAs/GaAs 계면의 전자 전달특성 분석)

  • Nam, Seung-Hun;Jung, Hak-Ki;Kim, Bong-Ryul
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.2
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    • pp.94-101
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    • 1989
  • Transport properties of 2DEG at AlGaAs/GaAs interface such as average electron energy, flight distance, each valley occupancy ratio, average electron velocity for various fields are investigated by MONTE-CARLO method. As the electric field increases, more electrons transit drastically from (000) valley to (000) upper valley. This phenomenon shows the nonstationary effect such as velocity overshoot. The duration of the transient decreases from about 1.4 psec for electric field E = 7KV/cm to about 0.7 psec for 12KV/cm. The average electron velocity during transient transport in 2DEG is about 8 times the steady-state velocity for E = 12KV/cm at room temperature. In comparison with bulk GaAs the peak velocity in the 2DEG is higher than that in even pure bulk GaAs at electric field E = 7 KV/cm. On the basis of the fact that the electrons in the 2DEG have larger peak velocity and shorter transient time of velocity than those in the bulk GaAs, it is suggested that the device with 2DEG may obtain higher mobility than that with bulk GaAs.

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