• 보건세계
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• v.41 no.9 s.457
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• pp.20-23
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• 1994

• Biomedical Science Letters
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• v.6 no.2
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• pp.141-149
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• 2000

The etiologic agents of haemorrhagic fever with renal syndrome (HFRS) in Korea are Hantaan and Seoul virus in the genus Hantavirus, family Bunyaviridae. Antibody titers of sera from HFRS patients against Hantaan virus were measured by immunofluorescent antibody technique (IFAT), enzyme-linked immunosorbent assay (ELISA), high density composite particle agglutination (HDPA) and plaque reduction neutralization test (PRNI). PRNT and nested reverse transcriptase polymerase chain reaction (nested RT-PCR) was used for serotypic differentiation of Hantaviruses against Hantaan and Seoul virus. Eight doubtful HFRS patients showed higher fluorescent, IgG ELISA, agglutination and neutralizing antibody titer by IFAT, ELISA IgG, HDPA and PRNT, respectively Five out of them showed high IgM antibody titer by IgM capture ELISA against Hantaan virus, remarkably. Fifteen HFRS patients showed higher fluorescent antibody titer by IFAT. In PRNT, 12 out of them showed high neutralizing antibody titer against HTNV, 2 against SEOV and 1 against both viruses. In nested RT-PCR using serotype specific-primer, 3 out of them showed positive against HTNV and 1 against SEOV.

• Journal of Yeungnam Medical Science
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• v.15 no.1
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• pp.1-11
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• 1998

• 보건세계
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• v.51 no.10 s.578
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• pp.22-24
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• 2004

무더운 여름 뒤에 오는 쾌적한 가을철에는 전염성 질환이 줄어들고 건강 유지가 비교적 쉽다. 그러나 야외 나들이 기회가 많아져 뱀이나 벌, 해충에 물리는 일이 많고 마른풀이나 잡초를 통해 전염되는 신증후(유행성)출혈열, 쯔쯔가무시증, 렙토스피라증 같은 `급성 열성 풍토병`이 가을철에 드물지 않게 생긴다. 가을철에 주의해야 할 질환과 치료법을 알아본다.

• The Journal of the Korean life insurance medical association
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• v.4 no.1
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• pp.16-23
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• 1987

• Journal of Yeungnam Medical Science
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• v.25 no.1
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• pp.41-49
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• 2008

Background : The central physiological derangement of hemorrhagic fever with renal syndrome (HFRS) caused by hantaan virus (HTNV) is a vascular dysfunction, manifested by hemorrhage, impaired vascular tone and increased vascular permeability. Platelet activating factor (PAF), whose actions are mediated through a specific receptor, is a potent bioactive lipid. PAF has diverse biological functions in the vascular system, such as increasing vascular permeability, adhesion of leukocytes to the endothelium and reduction of cardiac output, which result in hypotension and shock. The goal of the present study was to investigate whether PAF is involved in the pathogenesis of HFRS. For this purpose, we evaluated the effect of HTNV on the expression of PAF receptor (PAF-R) and on the activity of PAF-acetylhydrolase (PAF-AH) instead of PAF because PAF is rapidly degraded by PAF-AH in vivo. Materials and methods : To evaluate the expression of PAF-R, we performed reverse-transcription PCR, western blot and FACS analyses using HTNV-infected human umbilical vein endothelial cells (HUVECs) and non-infected (control) HUVECs. In addition, we measured the activity of plasma PAF-AH in HFRS patients and normal healthy persons. Results : The mRNA and protein expression of PAF-R was increased in HTNV-infected HUVECs compared with control HUVECs at 2 and 3 days post-infection (d.p.i.). FACS analysis showed that HTNV induced the surface expression of PAF-R in HUVECs from 2 d.p.i. The activity of plasma PAF-AH was 2.5-fold lower in HFRS patients than in normal healthy persons. Conclusion : Increased PAF-R expression by HTNV might increase the responsiveness to PAF in endothelial cells. Reduced PAF-AH activity in the blood of HFRS patients might delay PAF degradation. These results suggest that changes in PAF-R and PAF-AH by HTNV might influence to PAF activity and might be involved in the vascular dysfunction of HFRS.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.124-128
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• 1992

A total of 275 wild hats captured in Korea here examined 011 the possibility that they will harhor and spread the inkctious pathog.cns of Hantaan virus and Rickettsia. They possessed ontihotlies in 3.6'K) blood sera against Hantaan virus by the indirect in~~nunofluoresccnctec hnique and showed relatively high serum titers with a range of 16 to 1014X. whereus 13.7\ulcorner0 of them were positive to Rickettsia. dividing into 3.3% against R. ~.sur,~ugc~rnusllr.Ri.1 k, against R. siht)ric.n tick ~vphus,a nd 4.4% against R. thai tick pphus. respectively. 11 was first confirmed in Korean wild hats that arc infccted with Hantaan virus and Rickettsia agents. and play a role as vector.

• The Journal of Korean Society of Virology
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• v.26 no.1
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• pp.77-90
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• 1996

Nucleocapsid protein (NP)which exists in the particle of hantavirus and surrounds the viral RNA genome is one of the major structural proteins and plays role of antigen to elicit the antibody detected predorminantly right after infection of the virus in the patients of hemorragic fever with renal syndrome (HFRS)or experimental animals. NP is important target antigen in serological diagnostic system of HFRS utilizing whole antigens from the native virus particle, such as IFA, ELISA and Western blotting. Therefore, the preparation of this protein in the level of higher quantity and purity is desirasble for developed dianosis of the disease. The purpose of this study is the cloning of NP gene which exists in the S genome segment of Maaji (MAA) virus and expression of the gene to obtain qualified, genetically engineered NP to be utilized as an immunodiagnostic antigen. First of all, for the purpose of amplifing the MAA-NP gene by PCR, the specific primers were built from the known nucleotide sequence of Hantaan viral NP gene. The viral cDNA of the NP gene was synthesized by using the primers and RNase $H^-$ AMV reverse transcriptase. Thereafter, using this cDNA as a template, the NP gene was amplified specifically by Taq DNA polymrerase. The pT7blue (R)T-overhang vector systems were used for cloning of the amplified NP gene. The expression system was consisted of BL21 (DE3)pLysS and pET16b as a host and a plasmid repectively. Into Ndel site of pET16b, NP gene was ligated with cohesive end for the expression. Insertion of NP gene in the plasmid was confirmed by PCR and mini prep methods. For expression, IPTG was used and the expressed protein was characterized by Western blotting. The MAA-NP was expressed as the form of inclusion body (insoluble fraction)and the protein purified by affinity and metal chealating columns reacted specifically with the sera from patients of HFRS as to be tested by ELISA and Western blotting.

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.205-214
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• 1996

한탄바이러스의 내피 단백질 (N)은 이 바이러스에 대한 중요한 항원으로 작용하지만 신증후출혈열 예방과 관련된 작용은 명확히 알려져 있지 않다. 본 연구는 이러한 내피 단백질이 한탄바이러스에 대한 중화 항체를 유도할 수 있는가 하는 관점에서 수행되었다. 한탄바이러스의 내피 단백질을 대장균에서 용해된 형태로 발현하고 이를 단클론 항체를 이용한 면역친화 컬럼으로 분리 정제하였다. 정제된 내피 단백질을 기니픽에 면역하여 항혈청을 얻고 이것의 한탄바이러스에 대한 중화능력을 중화항체 플락 감소법 (plaque reduction neutralization test)을 이용하여 조사한 결과 최고 1:160의 중화능이 있음을 관찰하였다. 이는 한탄바이러스의 내피 단백질이 중화 항체를 유도할 수 있는 epitopes을 가지고 있음을 의리하며 이러한 생각은 본 연구에서 수행한 면역침강법과 N 단백질에 대한 단클론항체를 이용한 면역친화법을 통한 한탄바이러스의 정제 실험 결과에서도 뒷받침되고 있다.

• Journal of agricultural medicine and community health
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• v.19 no.2
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• pp.119-128
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• 1994

This study was performed on 302 cases of leptospirosis, tsutsugamushi disease and hemorrhagic fever with renal syndrome(HFRS) which occurred in Chonnam(Do) in 1991 through 1993. The results were as follows : 1. 81.8% of the patients with Leptospirosis and 54.5% of the HFRS patients were men while 61.4% of the patients with tsutsugamushi disease were women. 2. Most patients lived in rural areas(Gun), their educational level was elementary School or lower and their occupations were either farmer or jobless. 3. Peak ages were 40s for leptospirosis(36.4%) and 50s for tsutsugamushi disease and HFRS(32.9% and 36.4% respectively). 4. The high incidence areas of tsutsugamushi disease were northern, eastern and a diagonal line, from northeast to southwest, of the Chonnam area, and these are consistent with a mountainous district. 5. In monthly distribution leptospirosis was higher in Sep. and Oct., and tsutsugamushi disease and HFRS were higher in Oct. and Nov. 6. The first case is occurring and the last case shows up later than in past years.