• Journal of Food Hygiene and Safety
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• v.38 no.5
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• pp.279-286
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• 2023

Rapid and accurate detection of pathogenic bacteria is crucial for various applications, including public health and food safety. However, existing bacteria detection techniques have several drawbacks as they are inconvenient and require time-consuming procedures and complex machinery. Recently, the precision and versatility of CRISPR/Cas system has been leveraged to design biosensors that offer a more efficient and accurate approach to bacterial detection compared to the existing techniques. Significant research has been focused on developing biosensors based on the CRISPR/Cas system which has shown promise in efficiently detecting pathogenic bacteria or virus. In this review, we present a biosensor based on the CRISPR/Cas system that has been specifically developed to overcome these limitations and detect different pathogenic bacteria effectively including Vibrio parahaemolyticus, Salmonella, E. coli O157:H7, and Listeria monocytogenes. This biosensor takes advantage of the CRISPR/Cas system's precision and versatility for more efficiently accurately detecting bacteria compared to the previous techniques. The biosensor has potential to enhance public health and ensure food safety as the biosensor's design can revolutionize method of detecting pathogenic bacteria. It provides a rapid and reliable method for identifying harmful bacteria and it can aid in early intervention and preventive measures, mitigating the risk of bacterial outbreaks and their associated consequences. Further research and development in this area will lead to development of even more advanced biosensors capable of detecting an even broader range of bacterial pathogens, thereby significantly benefiting various industries and helping in safeguard human health

• Food Engineering Progress
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• v.15 no.1
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• pp.48-55
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• 2011

In this research, the near-infrared absorption from 1,100-2,300 nm was used to measure the content of capsaicinoids in the red-pepper powder by using the Acousto-optic tunable filters (AOTF) spectrometer with sample plate and sample rotating unit. Non-spicy red-pepper samples from one location (Younggwang-gun. Korea) were mixed with spicy one (var. Chungyang) to make samples separated by particle size (below 0.425 mm, 0.425-0.71 mm, and 0.71- 1.4 mm). The Partial Least Squares Regression (PLSR) model to predict the capsaicinoid content on particle sizes was developed with measured spectra by AOTF spectrometer and used to analyze the amount of capsaicinoids by HPLC. The PLSR Model of red-pepper powder of below 0.425 mm, 0.425-0.71 mm, and 0.71-1.4 mm with cross validation had ${R_V}^2$ = 0.948-0.979 and Standard Error of Prediction (SEP) = 6.56-7.94 mg%. The prediction error of smaller particle size of red-pepper powder was low. The best PLSR model was found in pretreatment of Range Normalization, Standard Normal Variate, and 1st Derivatives of red-pepper powder of below 1.4 mm with cross validation, having ${R_V}^2$ = 0.959 and SEP = 8.82 mg%.

• The Journal of the Korea institute of electronic communication sciences
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• v.16 no.4
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• pp.771-782
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• 2021

Regular or irregular situations such as traffic accidents, damage to road facilities, maintenance or repair work, and vehicle breakdowns occur frequently on highways. It is required to provide traffic services to drivers by promptly recognizing these regular or irregular situations, various techniques have been developed for rapidly collecting data and detecting abnormal traffic conditions to solve the problem. We propose a method that can be used for verification and demonstration of unexpected situation algorithms by establishing a system and developing algorithms for detecting unexpected situations on highways. For the detection of emergencies on expressways, a system was established by defining the expressway contingency and algorithm development, and a test bed was operated to suggest a method that can be used for verification and demonstration of contingency algorithms. In this study, a system was established by defining the unexpected situation and developing an algorithm to detect the unexpected situation on the highway, and a method that can be used verifying and demonstrating unexpected situations. It is expected to secure golden time for the injured by reducing the effectiveness of secondary accidents. Also predictable accidents can be reduced in case of unexpected situations and the detection time of unpredictable accidents.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.6B
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• pp.551-560
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• 2009

This paper describes a newly developed pollutant transport model named ARPTM which was designed to simulate the transport and characteristics of pollutant materials after an accidental spill in upstream of river system up to a given position in the downstream. In particular, the ARPTM incorporated ADCP data to compute longitudinal dispersion coefficient and advection velocity which are necessary to apply one-dimensional advection-dispersion equation. ARPTM was built on top of the geographic information system platforms to take advantage of the technology's capabilities to track geo-referenced processes and visualize the simulated results in conjunction with associated geographic layers such as digital maps. The ARPTM computes travel distance, time, and concentration of the pollutant cloud in the given flow path from the river network, after quickly finding path between the spill of the pollutant material and any concerned points in the downstream. ARPTM is closely connected with a recently developed GIS-based Arc River database that stores inputs and outputs of ARPTM. ARPTM thereby assembles measurements, modeling, and cyberinfrastructure components to create a useful cyber-tool for determining and visualizing the dynamics of the clouds of pollutants while dispersing in space and time. ARPTM is expected to be potentially used for building warning system for the transport of pollutant materials in a large basin.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.12
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• pp.6427-6433
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• 2013

For trains to run safely and quickly, the train should always follow the preceding train at a proper spacing. For this purpose, a certain distance between the stations is set for each block section. For the safe operation of trains in one block section, only one train service for an automatic block system is needed. The existing block system is composed an ABS, which is a linked track circuit and line sideway system through the interlocking system. The interlocking system is being replaced with a domestic electronic interlocking system. On the other hand, the block system still uses the relay format of an analog system, and is independently installed of the line sideway systems. Therefore, the existing block system has many problems in terms of construction and maintenance. In addition, the existing domestic line is used for ABS and LEU , which is installed separately, despite the train being controlled by the information of the same signal at the same location. This is not efficient in terms of each product price and the maintenance costs. This paper introduces an integrated electronic block system and the field test results. The field test was carried out through a periodic inspection performed eight times from January to late August.

• Progress in Medical Physics
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• v.3 no.1
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• pp.63-69
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• 1992

Recently, stereotactic radiosurgery plan is required with the information of 3-D image and dose distribution. The purpose of this research is to develop 3-D radiosurgery planning system using personal computer. The procedure of this research is based on three steps. The first step is to input the image information of the patient obtained from CT or MR scan into personal computer through on-line or digitizer. The position and shape of target are also transferred into computer using Angio or CT localization. The second step is to compute dose distribution on image plane, which is transformed into stereotactic frame coordinate. and to optimize dose distribution through the selection of optimal treatment parameters. The third step is to display both isodose distribution and patient image simultaneously using superimpose technique. This prototype of radiosurgery planning system was applied recently for several clinical cases. It was shown that our planning system is fast, accurate and efficient while making it possible to handle various kinds of image modelities such as angio, CT and MRI. It is also possible to develop 3-D planning system in radiation therapy using beam's eye view or CT simulation in future.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.10a
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• pp.441-444
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• 2007

In present, physical distribution industry fields are offering more convenient services using RFID, but there is plenty of room for improvement. And then, utilizing advantages of RFID which quick and simple manages goods and GPS which gets information of position at the present, we implemented a physical distribution management system can manages the information for distribution process of goods easier. We can get much information that the number of loaded goods, the data of goods, the state of distribution, whether or not missing, etc. as attached a RFID reader to the truck. and when truck is moving, we can also obtain much information consumer want that the real time data of position, distribution routes, etc. for loaded goods as received a latitude and longitude from GPS. These information have recorded, managed, and linked Google map, we can grasp the distribution information of goods on World Wide Web service. Because this service is focus on the image not the text can give the information required by the consumer on visual, it is different from the existing service. At this point of time that the RFID and GPS have used in overall industry, If these services have researched and developed with transportation, tour, etc. industry as well as physical distribution, it is possible to utilize more widely.

• Fire Science and Engineering
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• v.29 no.6
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• pp.51-56
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• 2015

In Korea, measures to maintain sustainable fire safety performance for apartment buildings are insufficient in terms of fire-fighting products, skilled personnel, and maintenance status. Also, because of the particular features of a fire compartment, it has structural problems that are very likely to cause damage to human life when a fire occurs. Currently, problems with the fire supervisory system installed in an apartment building cannot be checked in real time, so it is difficult to identify the location of a fire accurately. Protected areas are also not assigned to each household, and residents cannot be clearly informed of the occurrence of a fire. As a consequence, safety evacuation cannot be secured. In addition, it is impossible to test the operation performance for water detectors in sprinkler fire extinguishing systems outside of the household. Therefore, an experiment was conducted to evaluate the performance of a remote fire supervisory system. The results show that the system satisfies all performance requirements. Also, an household alarm system was installed in each household to alert of any occurrence of a fire accurately, and the performance of the alarm system was improved to ensure that residents were quickly evacuated.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.286-290
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• 2008

Many important traits have been tagged allowing plant breeders to apply marker assisted selection (MAS) in rice. PCR itself is simple to set up, and requires little hands-on time. However, a crucial limiting step of MAS programs is the reliable and efficient extraction of DNA which can be performed on thousands of individuals. In this study, We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract DNA from leaf tissues for suitable MAS in rice. We followed the standard 2% CTAB extraction method in all the procedure. In addition we used the 1.2 ml 8-strip tube instead of 1.5 ml E-tubes to fit the 8-multichannel pipette and employ the 96 well plate to use the swing bucket centrifuge. Our modified CTAB DNA extraction method offers several advantages with respect to traditional and simple methods. 1) adult leaf samples collected in paddy field are applicable. 2) 96 leaf samples can be homogenized only one-time by using tungsten carbonate bead and 96well block. 3) semiautomatic loading method using 8-multichannel pipette from DNA extraction to electrophoresis of PCR products. 4) our system can extract about 400 leaf samples per day by only one technicion. Therefore, this method could be useful for marker assisted breeding in rice.

• Smart Media Journal
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• v.11 no.3
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• pp.54-61
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• 2022

With the increase in the types of information systems managed by public institutions and companies, a security certification system is being implemented in Korea to quickly respond to vulnerabilities that may arise due to insufficient security checks. The korea security evaluation system, such as ISMS-P, performs a systematic security evaluation for each category by dividing the categories for technical inspection items. NIST in the United States has developed SCAP that can create security checklists and automate vulnerability checks, and the security checklists used for SCAP can be written in OVAL. Each manufacturer prepares a security check list and shares it through the SCAP community, but it's difficult to use it in Korea because it is not categorized according to the korea security evaluation system. Therefore, in this paper, we present a mechanism to categorize the OVAL definition, which is an inspection item written in OVAL, to apply SCAP to the korea security evaluation system. It was shown that 189 out of 230 items of the Red Hat 8 STIG file could be applied to the korea security evaluation system, and the statistics of the categorized Redhat definition file could be analyzed to confirm the trend of system vulnerabilities by category.